公开(公告)号：US09920350B2

公开(公告)日：2018-03-20

申请号：US14368711

申请日：2012-12-19

Applicant: 3M INNOVATIVE PROPERTIES COMPANY

Inventor： Chirstine A. Binsfeld ,  Patrick A. Mach ,  Mara S. Celt ,  Adam J. Stanenas

IPC: C12Q1/10 ,  C12Q1/04

CPC classification number: C12Q1/10 ,  C12Q1/045 ,  G01N2333/255 ,  Y02A50/451

Abstract: A composition is provided for detecting a Salmonella microorganism in sample. The composition comprises at least one first selective agent that inhibits the growth of Gram-positive microorganisms, a first differential indicator system comprising at least one first differential indicator compound that is converted to a first detectable product by a Salmonella microorganism, and a second differential indicator system comprising a second differential indicator compound that is converted by urease enzyme activity to a second detectable product. Optionally, the composition may comprise a third differential indicator system comprising a third differential indicator compound that is converted by a β-galactosidase enzyme activity to a third detectable product. Methods of using the composition to detect a Salmonella microorganism are also provided.