公开(公告)号：WO1986006385A1

公开(公告)日：1986-11-06

申请号：PCT/US1986000723

申请日：1986-04-09

Applicant: AMGEN

Inventor： AMGEN ,  ARAKAWA, Tsutomu

IPC: C07K15/26

CPC classification number: C07K1/1136 ,  C07K14/57 ,  Y10S930/142

Abstract: A composition of matter consisting of recombinant human immune interferon having a near-UV circular dichroic spectrum in aqueous solution at neutral pH with positive bands at about 259 nm, 266 nm, 280 nm, and 287 nm, and with shoulders at about 270 nm and 292 nm. Also disclosed is a method for purifying human immune interferon in which proper refolding of the interferon is accomplished by unfolding in a denaturant, such as urea, dilution in ammonium acetate to approximately 0.18 mg/ml of interferon (or less), and dialysis of the solution. The properly folded, purified product which results has a four- to eight-fold greater activity than the aggregate which otherwise results.

公开(公告)号：WO1984004330A1

公开(公告)日：1984-11-08

申请号：PCT/US1984000601

申请日：1984-04-18

Applicant: AMGEN

Inventor： AMGEN ,  BITTER, Grant, A.

IPC: C12P21/02

CPC classification number: C12N15/625 ,  C07K14/675 ,  C07K2319/02 ,  C07K2319/036 ,  C07K2319/75 ,  C12N15/81

Abstract: Recombinant methods and materials for use in securing production of exogenous (e.g., mammalian) polypeptides in yeast cells wherein hybrid precursor peptides susceptible to intracellular processing are formed and such processing results in secretion of desired polypeptides. In a presently preferred form, the invention provides transformation vectors with DNA sequences coding for yeast synthesis of hybrid precursor polypeptides comprising both an endogenous yeast polypeptide sequence (e.g., that of a precursor polypeptide associated with yeast-secreted mating factor alpha and an exogenous polypeptide sequence (e.g., human beta-endorphin). Transformation of yeast cells with such DNA vectors results in secretion of desired exogenous polypeptide (e.g., substances displaying one or more of the biological properties of beta-endorphin).