公开(公告)号：US06741730B2

公开(公告)日：2004-05-25

申请号：US10307712

申请日：2002-12-02

Applicant: J. Richard Rahn ,  Alan C. Nelson

Inventor： J. Richard Rahn ,  Alan C. Nelson

IPC: G06K900

CPC classification number: G06T11/006 ,  G01N23/046 ,  G01N2223/419 ,  G06T2211/421

Abstract: Two or more two-dimensional Fourier transforms are acquired from different perspectives of a three-dimensional object region. A three-dimensional Fourier transform is then constructed using tomographic methods, permitting the application of image analysis algorithms analogous to those used for two-dimensional images.

Abstract translation: 从三维物体区域的不同角度获取两个以上的二维傅立叶变换。 然后使用断层摄影方法构建三维傅里叶变换，允许应用与用于二维图像的图像分析算法类似的图像分析算法。

公开(公告)号：US06591003B2

公开(公告)日：2003-07-08

申请号：US10260908

申请日：2002-09-30

Applicant: Chee-Wui Chu ,  Alan C. Nelson

Inventor： Chee-Wui Chu ,  Alan C. Nelson

IPC: G06K900

CPC classification number: G01N23/046 ,  G01N15/147 ,  G01N21/05 ,  G01N21/4795 ,  G01N2223/419 ,  G06T11/006

Abstract: Three dimensional reconstruction of an object of interest moving at a constant velocity. The object of interest is centered. The object of interest is imaged with optical point sources located at multiple projection angles around the object of interest, in cooperation with opposing time delay and integration (TDI) image sensors located at a distance from the objects of interest such that there is no focal plane within the objects of interest during imaging. Each of the TDI sensors has a line transfer rate synchronized to the constant velocity of the objects of interest.

公开(公告)号：US5402460A

公开(公告)日：1995-03-28

申请号：US100778

申请日：1993-08-02

Applicant: Roger H. Johnson ,  Alan C. Nelson ,  Robert M. Fisher

Inventor： Roger H. Johnson ,  Alan C. Nelson ,  Robert M. Fisher

IPC: G01N23/04 ,  G21K7/00 ,  G01N23/087 ,  A61B6/03 ,  G01N23/083

CPC classification number: G01N23/046 ,  G21K7/00 ,  A61B6/583 ,  G01N2223/419 ,  Y10S378/901

Abstract: A microtomographic system (10) for generating high-resolution, three dimensional images of a specimen (16) is disclosed. The microtomograph system includes an x-ray generator (12) that produces an x-ray beam (14), a specimen holder (18) that holds the specimen in the beam, and an x-ray detector (20) that measures the attenuation of the beam through the specimen. Two projections of each view of the specimen are made with this microtomographic system. Each projection is made with a different intensity x-ray beam. After the projections of one view of the specimen are made, the specimen is rotated on the specimen holder and another set of projections are made. The projections of each view of the specimen are analyzed together to provide a quantitative indication of the phase fraction of the material comprising the specimen. The projections of the different views are combined to provide a three-dimensional image of the specimen.

Abstract translation: 公开了一种用于产生样本（16）的高分辨率三维图像的微图系统（10）。 显微图像系统包括产生X射线束（14）的X射线发生器（12），将样本保持在束中的样本保持器（18）和测量衰减的X射线检测器（20） 的光束通过样品。 用该微图系统制作样本的每个视图的两个突出部分。 每个投影都由不同强度的X射线束制成。 在制作样本的一个视图的突起之后，将样本在样本保持器上旋转，并且制作另一组突起。 一起分析样品的每个视图的突起，以提供包含样品的材料的相分数的定量指示。 组合不同视图的投影以提供样本的三维图像。

公开(公告)号：US08143600B2

公开(公告)日：2012-03-27

申请号：US12032959

申请日：2008-02-18

Applicant: Eric J. Seibel ,  Alan C. Nelson ,  Mark E. Fauver ,  J. Richard Rahn

Inventor： Eric J. Seibel ,  Alan C. Nelson ,  Mark E. Fauver ,  J. Richard Rahn

IPC: G01N21/64

CPC classification number: G01N21/4795 ,  G01N15/1436 ,  G01N2015/1445 ,  G01N2021/1787 ,  G06T11/006

Abstract: A method for 3D imaging of cells in an optical tomography system includes moving a biological object relatively to a microscope objective to present varying angles of view. The biological object is illuminated with radiation having a spectral bandwidth limited to wavelengths between 150 nm and 390 nm. Radiation transmitted through the biological object and the microscope objective is sensed with a camera from a plurality of differing view angles. A plurality of pseudoprojections of the biological object from the sensed radiation is formed and the plurality of pseudoprojections is reconstructed to form a 3D image of the cell.

Abstract translation: 用于光学断层摄影系统中的细胞的3D成像的方法包括相对于显微镜物镜移动生物体以呈现不同的视角。 用具有限制在150nm和390nm之间的波长的光谱带宽的辐射照射该生物物体。 利用摄像机从多个不同的视角检测透过生物物体和显微镜物镜的辐射。 形成来自感测辐射的生物物体的多个伪投影，并重建多个伪投影以形成该细胞的3D图像。

公开(公告)号：US20090208072A1

公开(公告)日：2009-08-20

申请号：US12032959

申请日：2008-02-18

Applicant: Eric J. Seibel ,  Alan C. Nelson ,  Mark E. Fauver ,  J. Richard Rahn

Inventor： Eric J. Seibel ,  Alan C. Nelson ,  Mark E. Fauver ,  J. Richard Rahn

IPC: G06K9/00

CPC classification number: G01N21/4795 ,  G01N15/1436 ,  G01N2015/1445 ,  G01N2021/1787 ,  G06T11/006

Abstract: A method for 3D imaging of cells in an optical tomography system includes moving a biological object relatively to a microscope objective to present varying angles of view. The biological object is illuminated with radiation having a spectral bandwidth limited to wavelengths between 150 nm and 390 nm. Radiation transmitted through the biological object and the microscope objective is sensed with a camera from a plurality of differing view angles. A plurality of pseudoprojections of the biological object from the sensed radiation is formed and the plurality of pseudoprojections is reconstructed to form a 3D image of the cell.

Abstract translation: 用于光学断层摄影系统中的细胞的3D成像的方法包括相对于显微镜物镜移动生物体以呈现不同的视角。 用具有限制在150nm和390nm之间的波长的光谱带宽的辐射照射该生物物体。 利用摄像机从多个不同的视角检测透过生物物体和显微镜物镜的辐射。 形成来自感测辐射的生物物体的多个伪投影，并重建多个伪投影以形成该细胞的3D图像。

公开(公告)号：US07569789B2

公开(公告)日：2009-08-04

申请号：US11377032

申请日：2006-03-16

Applicant: Jon W. Hayenga ,  Alan C. Nelson

Inventor： Jon W. Hayenga ,  Alan C. Nelson

IPC: B07C5/00

CPC classification number: B01L3/502761 ,  B01L3/0293 ,  B01L3/502776 ,  B01L3/502784 ,  B01L2200/027 ,  B01L2200/0636 ,  B01L2300/0864 ,  B01L2400/0487 ,  G01N15/1459 ,  G01N15/1463 ,  G01N2015/149

Abstract: An apparatus and method for sorting particles in a laminar flow microfluidic channel includes a cantilevered coaxial flow injector in a microfluidic device, the cantilevered coaxial flow injector including an elongated cantilever element integrated into the microfluidic device. A coaxial channel runs through the elongated cantilever element, where coaxial channel is sized to pass particles of a predetermined size. An actuator is coupled to the elongated cantilever element, for actuating said elongated cantilever element.

Abstract translation: 用于在层流微流体通道中分选颗粒的装置和方法包括微流体装置中的悬臂同轴流注射器，所述悬臂同轴流动注射器包括集成到微流体装置中的细长悬臂元件。 同轴通道穿过细长的悬臂元件，其中同轴通道的尺寸适于通过预定尺寸的颗粒。 致动器联接到细长悬臂元件，用于致动所述细长悬臂元件。

公开(公告)号：US06519355B2

公开(公告)日：2003-02-11

申请号：US10055066

申请日：2002-01-22

Applicant: Alan C. Nelson

Inventor： Alan C. Nelson

IPC: G06K900

CPC classification number: G06K9/00134 ,  G01N15/147 ,  G01N15/1475 ,  G01N21/05 ,  G01N23/046 ,  G01N2223/419 ,  G01N2223/612 ,  G06T7/0012 ,  G06T2207/10101 ,  G06T2207/30024

Abstract: A system and method for rapidly detecting cells of interest using multi-dimensional, highly quantitative, nuclear and cytoplasmic densitometric features (NDFs and CDFs) includes a flow optical tomography (FOT) instrument capable of generating various optical projection images (or shadowgrams) containing accurate density information from a cell, a computer and software to analyze and reconstruct the projection images into a multi-dimensional data set, and automated feature collection and object classifiers. The system and method are particularly useful in the early detection of cancers such as lung cancer using a bronchial specimen from sputum or cheek scrapings and cervical/ovarian cancer using a cervical scraping, and the system can be used to detect rare cells in specimens including blood.

公开(公告)号：US06252979B1

公开(公告)日：2001-06-26

申请号：US08485182

申请日：1995-06-07

Applicant: Shih-Jong J. Lee ,  Alan C. Nelson ,  Carl E. Youngmann

Inventor： Shih-Jong J. Lee ,  Alan C. Nelson ,  Carl E. Youngmann

IPC: G06K900

CPC classification number: G06K9/00127

Abstract: An interactive process for sorting biological specimens includes the steps of processing a biological specimen to provide an analysis score, sorting the biological specimens according to the analysis score into categories including: clearly normal, interactive review, and microscopy review, and selecting a field-of-view (FOV) for the biological specimens sorted into the interactive review category.

Abstract translation: 用于分选生物标本的交互过程包括处理生物样本以提供分析得分的步骤，根据分析分数将生物样本分类为包括：明显正常的，互动的评论和显微镜检查，以及选择一个 将视野（FOV）作为生物标本分为互动评价类别。

公开(公告)号：US6122397A

公开(公告)日：2000-09-19

申请号：US888115

申请日：1997-07-03

Applicant: Shih-Jong J. Lee ,  Alan C. Nelson

Inventor： Shih-Jong J. Lee ,  Alan C. Nelson

IPC: G01B11/30 ,  G01M11/00 ,  G01N21/956 ,  G01N21/958 ,  G06T1/00 ,  G06T7/00 ,  H01L21/66 ,  G06K9/00

CPC classification number: G06T7/001 ,  G06T2207/30121 ,  G06T2207/30148

Abstract: Image primitive based maskless semiconductor wafer and liquid crystal display panel inspection by the characterization of wafer patterns. Potential defects are detected as exceptions to the rules of general semiconductor surface pattern structure. The wafer or liquid crystal display, lcd, structure is encoded into multiple profiles of a set of primitive characterization modules. Primitive profiles are correlated with potential defects along with aligned pattern images for surface component to surface component, lcd active matrix element to lcd active matrix element, comparison and further refines the results using data from multiple surface components or lcd active matrix elements. Multiple stage defect classification is applied to the potential defects to reject false defects. Multiple layer correlation and automatic learning enhance and tailor detection rules during a ramp-up stage. There is a dramatic reduction of false and nuisance defects and a high sensitivity to critical defects. The highly robust method is not sensitive to factors such as metal grain structure and imperfect alignment. Automatic learning tailors the inspection system for a specific semiconductor surface design and manufacturing process.

Abstract translation: 基于图像原始的无掩模半导体晶片和液晶显示面板通过晶圆图案的表征进行检查。 潜在的缺陷被检测为一般半导体表面图案结构规则的例外。 晶片或液晶显示器，lcd，结构被编码成一组原始表征模块的多个轮廓。 原始曲线与潜在的缺陷以及表面成分到表面分量，lcd有源矩阵元素到lcd有源矩阵元素的对齐图案图像相关，并且使用来自多个表面分量或lcd有源矩阵元素的数据进一步优化结果。 多级缺陷分类应用于潜在的缺陷以排除假缺陷。 多层相关和自动学习在加速阶段提高和定制检测规则。 虚假和滋扰缺陷大大减少，对关键缺陷有很高的敏感性。 高度鲁棒的方法对金属晶粒结构和不完全对准等因素不敏感。 自动学习为特定的半导体表面设计和制造过程定制了检测系统。

公开(公告)号：US4720633A

公开(公告)日：1988-01-19

申请号：US820537

申请日：1986-01-17

Applicant: Alan C. Nelson

Inventor： Alan C. Nelson

IPC: H01J37/20 ,  G01N23/00

CPC classification number: H01J37/20 ,  H01J2237/2002 ,  H01J2237/2608

Abstract: A scanning electron microscope useful for obtaining microscopic data or images of wet specimens is provided with comprises an electron source capable of emitting a beam of electrons; an electron optical vacuum column with means for focussing the beam of electrons; means for scanning the focussed beam of electrons across a specimen; a differentially pumped aperture column attached to the electron optical vacuum column and having at least two walls perpendicular to the sides of the differentially pumped aperture column defining a suitable series of pressure gradients, each wall having an aperture aligned to permit the beam of electrons to pass through said differentially pumped aperture column; a specimen chamber which may be maintained at normal atmospheric pressure; a specimen mount; means of preventing the buildup of negative charge on the surface of the specimen; and a detector and image recording system.

Abstract translation: 提供了用于获得湿样品的微观数据或图像的扫描电子显微镜，其包括能够发射电子束的电子源; 具有用于聚焦电子束的装置的电子光学真空柱; 用于扫描聚焦的电子束穿过样本的装置; 连接到电子光学真空塔的差分泵浦孔径柱，并且具有至少两个垂直于差分泵浦孔径柱的侧壁的壁，其限定了适当的一系列压力梯度，每个壁具有孔径对准以允许电子束通过 通过所述差分泵浦孔径柱; 可以保持在正常大气压下的试样室; 样品座 防止样品表面积极负电荷的手段; 以及检测器和图像记录系统。