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公开(公告)号：NO20021906A
公开(公告)日：2002-04-26
申请号：NO20021906
申请日：2002-04-23
Applicant: BASF AG
Inventor： SCHMID ANDREAS , WITHOLD BERNARD , HAUER BERNHARD , BUEHLER BRUNO
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公开(公告)号：NO20021906D0
公开(公告)日：2002-04-23
申请号：NO20021906
申请日：2002-04-23
Applicant: BASF AG
Inventor： SCHMID ANDREAS , WITHOLD BERNARD , HAUER BERNHARD , BUEHLER BRUNO
Abstract: Production of aromatic aldehyde or carboxylic acid (I) by culturing a microorganism that expresses xylene mono-oxygenase (XMO) or alkane mono-oxygenase (AMO) in a medium containing an aromatic substrate (II). Production of aromatic aldehyde or carboxylic acid of formula (I) by culturing a microorganism that expresses xylene mono-oxygenase (XMO) or alkane mono-oxygenase (AMO) in a medium containing an aromatic substrate of formula (II). [Image] Ar : monocyclic aromatic ring, optionally with one or more substituents; R 1>formyl or carboxy; n : 0-15; R 2>vinyl or (CH 2) m-R 3>, also where R 1>= carboxy, R 2>may be (CH 2) n-CHO; m : n+1; and R 3>hydrogen or hydroxy. Independent claims are also included for the following: (a) recombinant microorganism harboring an expression vector that contains, under control of the alk-regulatory system of Pseudomonas oleovoransGpol, the XMO-encoding genes xylM and xylA, or the AMO-encoding genes alkB, alkG and alkT; and (b) expression construct containing the genes specified in (a), operably linked to the alk-regulatory system.
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公开(公告)号：EE05114B1
公开(公告)日：2008-12-15
申请号：EEP200200646
申请日：2001-05-16
Applicant: BASF AG
Inventor： SCHMID ANDREAS , HOLLMANN FRANK , HAUER BERNHARD , ZELINSKI THOMAS
Abstract: A method comprising the indirect electrochemical regeneration of NAD(P)H in enzymatic substrate conversions which are catalyzed by monooxygenases, for example, is described. In particular, a method for the electroenzymatic preparation of 2,3-dihydroxyphenyl derivatives, which is catalyzed by the enzyme 2-hydroxybiphenyl 3-monooxygenase and in which simultaneously the NAD + formed by reductive cleavage of oxygen is electrochemically reduced, is described.
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公开(公告)号：DE50111739D1
公开(公告)日：2007-02-08
申请号：DE50111739
申请日：2001-05-16
Applicant: BASF AG
Inventor： STECKHAN EBERHARD , SCHMID ANDREAS , HOLLMANN FRANK , HAUER BERNHARD , ZELINSKI THOMAS
Abstract: A method comprising the indirect electrochemical regeneration of NAD(P)H in enzymatic substrate conversions which are catalyzed by monooxygenases, for example, is described. In particular, a method for the electroenzymatic preparation of 2,3-dihydroxyphenyl derivatives, which is catalyzed by the enzyme 2-hydroxybiphenyl 3-monooxygenase and in which simultaneously the NAD + formed by reductive cleavage of oxygen is electrochemically reduced, is described.
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公开(公告)号：NO20065545A
公开(公告)日：2006-12-12
申请号：NO20065545
申请日：2006-12-01
Applicant: BASF AG
Inventor： HAUER BERNHARD , SCHMID ANDREAS , HOLLMAN FRANK , HOFSTETTER KARIN , HABICHER TILO
CPC classification number: C12P1/00 , C12N9/0071 , C12P7/00
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Patent Agency Ranking
公开(公告)号：AU2005245554A1
公开(公告)日：2005-12-01
申请号：AU2005245554
申请日：2005-05-11
Applicant: BASF AG
Inventor： HOFSTETTER KARIN , HOLLMANN FRANK , HABICHER TILO , SCHMID ANDREAS , HAUER BERNHARD
Abstract: Process for an enzymatic oxygenation catalyzed by a FAD-dependant monooxygenase and direct electrochemical regeneration of the FAD-dependant monooxygenase
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公开(公告)号：NO20025503L
公开(公告)日：2002-11-15
申请号：NO20025503
申请日：2002-11-15
Applicant: BASF AG
Inventor： SCHMID ANDREAS , HOLLMANN FRANK , HAUER BERNHARD , ZELINSKI THOMAS , STECKHAN EBERHARD
Abstract: A method comprising the indirect electrochemical regeneration of NAD(P)H in enzymatic substrate conversions which are catalyzed by monooxygenases, for example, is described. In particular, a method for the electroenzymatic preparation of 2,3-dihydroxyphenyl derivatives, which is catalyzed by the enzyme 2-hydroxybiphenyl 3-monooxygenase and in which simultaneously the NAD + formed by reductive cleavage of oxygen is electrochemically reduced, is described.
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公开(公告)号：CA2409339A1
公开(公告)日：2002-11-15
申请号：CA2409339
申请日：2001-05-16
Applicant: BASF AG
Inventor： ZELINSKI THOMAS , SCHMID ANDREAS , HOLLMANN FRANK , HAUER BERNHARD , STECKHAN EBERHARD
Abstract: A method comprising the indirect electrochemical regeneration of NAD(P)H in enzymatic substrate conversions which are catalyzed by monooxygenases, for example, is described. In particular, a method for the electroenzymatic preparation of 2,3-dihydroxyphenyl derivatives, which is catalyzed by the enzyme 2-hydroxybiphenyl 3-monooxygenase and in which simultaneously the NAD + formed by reductive cleavage of oxygen is electrochemically reduced, is described.
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公开(公告)号：DE10024314A1
公开(公告)日：2001-11-22
申请号：DE10024314
申请日：2000-05-17
Applicant: BASF AG
Inventor： HAUER BERNHARD , ZELINSKI THOMAS , STECKHAN EBERHARD , HOLLMANN FRANK , SCHMID ANDREAS
IPC: C12M1/40 , C12P1/00 , C12P7/22 , C25B3/25 , C12P13/00 , C12M1/42 , C25B3/04 , C07H19/207 , C07C255/53 , C07C39/08 , C07C39/10 , C07C39/15 , C07C39/28
Abstract: NAD(P)H is regenerated by electrochemical reduction from NAD(P) formed in an oxidative enzymatic reaction of an oxidizable substrate in the presence of NAD(P)H. Regeneration of NAD(P)H from NAD(P) formed in a NAD(P)H-consumed oxidative enzymatic reaction of an oxidizable substrate in the presence of NAD(P)H comprises electrochemical reduction of the NAD(P) .
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公开(公告)号：DE19951768A1
公开(公告)日：2001-05-03
申请号：DE19951768
申请日：1999-10-27
Applicant: BASF AG
Inventor： HAUER BERNHARD , WITHOLT BERNHARD , SCHMID ANDREAS , BUEHLER BRUNO
Abstract: The invention relates to a method for the step-wise oxidation of aromatic compounds to the corresponding aldehyde and/or carboxylic acid derivatives using genetically engineered microorganisms that express xylol monooxygenase or alkane monooxygenase.
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