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    SINGLE-PARTICLE ANALYSIS OF PARTICLE POPULATIONS
    21.
    发明授权
    SINGLE-PARTICLE ANALYSIS OF PARTICLE POPULATIONS 有权
    粒子群的单粒子分析

    公开(公告)号：EP2852682B1

    公开(公告)日：2017-10-04

    申请号：EP13726975.9

    申请日：2013-05-21

    Applicant: Fluidigm Corporation

    Inventor： ANDERSON, Megan ,  CHEN, Peilin ,  FOWLER, Brian ,  KAPER, Fiona ,  LEBOFSKY, Ronald ,  MAY, Andrew

    IPC: C12Q1/68

    CPC classification number: C12Q1/6813 ,  B01D15/08 ,  C12Q1/6806 ,  C12Q2563/159 ,  C12Q2563/179 ,  C12Q2565/629

    Abstract: In certain embodiments, the invention provides methods and devices for assaying single particles in a population of particles, wherein at least two parameters are measured for each particle. One or more parameters can be measured while the particles are in the separate reaction volumes. Alternatively or in addition, one or more parameters can be measured in a later analytic step, e.g., where reactions are carried out in the separate reaction volumes and the reaction products are recovered and analyzed. In particular embodiments, one or more parameter measurements are carried out “in parallel,” i.e., essentially simultaneously in the separate reaction volumes.

    Abstract translation: 在某些实施方案中，本发明提供了用于测定颗粒群中的单个颗粒的方法和装置，其中对于每个颗粒测量至少两个参数。 当粒子处于单独的反应体积中时，可以测量一个或多个参数。 可替代地或另外地，可以在稍后的分析步骤中测量一个或多个参数，例如，在单独的反应体积中进行反应并且回收和分析反应产物。 在特定的实施方案中，“并行”进行一个或多个参数测量，即基本上同时在单独的反应体积中进行。

    MICROFLUIDIC PARTICLE-ANALYSIS SYSTEMS
    25.
    发明公开
    MICROFLUIDIC PARTICLE-ANALYSIS SYSTEMS 审中-公开
    MIKROFLUIDISCHE PARTIKELANALYSESYSTEME

    公开(公告)号：EP1499706A2

    公开(公告)日：2005-01-26

    申请号：EP03721509.2

    申请日：2003-04-01

    Applicant: Fluidigm Corporation

    Inventor： CHOU, Hou-Pu ,  DARIDON, Antoine ,  FARRELL, Kevin ,  FOWLER, Brian ,  HAO, Cunsheng (Casey) ,  JAVADI, Shervin ,  LIAU, Yish-Hann ,  MANGER, Ian, D. ,  NASSEF, Hany, Ramez ,  NORTON, Pierce ,  THRONDSET, William

    IPC: C12M1/34

    CPC classification number: C12M1/34 ,  B01L3/502738 ,  B01L3/502746 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2200/16 ,  B01L2300/06 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0681 ,  B01L2300/0861 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/088 ,  B01L2300/0887 ,  B01L2300/0893 ,  B01L2300/123 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/0481 ,  B01L2400/0622 ,  B01L2400/0655 ,  C12M21/06 ,  C12M23/16 ,  G01N15/1484 ,  G01N33/4833 ,  G01N2015/008 ,  G01N2015/0288 ,  G01N2015/149 ,  G01N2015/1493 ,  G02B21/32

    Abstract: The invention provides systems, including apparatus, methods, and kits, for the microfluidic manipulation and/or detection of particles, such as cells and/or beads. The invention provides systems, including apparatus, methods, and kits, for the microfluidic manipulation and/or analysis of particles, such as cells, viruses, organelles, beads, and/or vesicles. The invention also provides microfluidic mechanisms for carrying out these manipulations and analyses. These mechanisms may enable controlled input, movement/positioning, retention/localization, treatment, measurement, release, and/or output of particles. Furthermore, these mechanisms may be combined in any suitable order and/or employed for any suitable number of times within a system. Accordingly, these combinations may allow particles to be sorted, cultured, mixed, treated, and/or assayed, among others, as single particles, mixed groups of particles, arrays of particles, heterogeneous particle sets, and/or homogeneous particle sets, among others, in series and/or in parallel. In addition, these combinations may enable microfluidic systems to be reused. Furthermore, these combinations may allow the response of particles to treatment to be measured on a shorter time scale than was previously possible. Therefore, systems of the invention may allow a broad range of cell and particle assays, such as drug screens, cell characterizations, research studies, and/or clinical analyses, among others, to be scaled down to microfluidic size. Such scaled-down assays may use less sample and reagent, may be less labor intensive, and/or may be more informative than comparable macrofluidic assays.

    Abstract translation: 本发明提供了用于微流控操作和/或检测诸如细胞和/或珠粒的装置，方法和试剂盒的系统。 本发明提供了用于微粒操作和/或分析颗粒如细胞，病毒，细胞器，珠粒和/或囊泡的装置，方法和试剂盒的系统。 本发明还提供用于进行这些操作和分析的微流体机构。 这些机制可以实现颗粒的控制输入，移动/定位，保留/定位，处理，测量，释放和/或输出。 此外，这些机构可以以任何合适的顺序组合和/或在系统内任何适当次数使用。 因此，这些组合可以允许将颗粒分类，培养，混合，处理和/或测定为单颗粒，颗粒混合组，颗粒阵列，非均匀颗粒组和/或均相颗粒组，其中 其他，串联和/或并行。 此外，这些组合可以使得微流体系统能够重复使用。 此外，这些组合可以允许在比以前可能的更短的时间尺度上测量颗粒对治疗的反应。 因此，本发明的系统可以允许广泛范围的细胞和颗粒测定，例如药物筛选，细胞特征化，研究研究和/或临床分析等，以缩小至微流体大小。 这种按比例缩小的测定可能使用较少的样品和试剂，可能较少劳动密集，和/或可比比较的大流式细胞仪更有信息。

    METHODS, SYSTEMS, AND DEVICES FOR MULTIPLE SINGLE-CELL CAPTURING AND PROCESSING USING MICROFLUIDICS
    26.
    发明公开
    METHODS, SYSTEMS, AND DEVICES FOR MULTIPLE SINGLE-CELL CAPTURING AND PROCESSING USING MICROFLUIDICS 审中-公开

    公开(公告)号：EP3627140A1

    公开(公告)日：2020-03-25

    申请号：EP19189921.0

    申请日：2013-02-28

    Applicant: Fluidigm Corporation

    Inventor： FOWLER, Brian ,  KIMBALL, Jake ,  MAUNG, Myo Thu ,  MAY, Andrew ,  NORRIS, Michael C. ,  TOPPANI, Dominique G. ,  UNGER, Marc A. ,  WANG, Jing ,  WEST, Jason A. A.

    IPC: G01N15/10 ,  C12Q1/6806

    Abstract: Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing. Reaction products may be harvested and/or further analyzed in some cases.

    SINGLE-PARTICLE ANALYSIS OF PARTICLE POPULATIONS
    27.
    发明公开
    SINGLE-PARTICLE ANALYSIS OF PARTICLE POPULATIONS 有权
    EINZELPARTIKELANALYSE VON TEILCHENPOPULATIONEN

    公开(公告)号：EP2852682A2

    公开(公告)日：2015-04-01

    申请号：EP13726975.9

    申请日：2013-05-21

    Applicant: Fluidigm Corporation

    Inventor： ANDERSON, Megan ,  CHEN, Peilin ,  FOWLER, Brian ,  KAPER, Fiona ,  LEBOFSKY, Ronald ,  MAY, Andrew

    IPC: C12Q1/68

    CPC classification number: C12Q1/6813 ,  B01D15/08 ,  C12Q1/6806 ,  C12Q2563/159 ,  C12Q2563/179 ,  C12Q2565/629

    Abstract: In certain embodiments, the invention provides methods and devices for assaying single particles in a population of particles, wherein at least two parameters are measured for each particle. One or more parameters can be measured while the particles are in the separate reaction volumes. Alternatively or in addition, one or more parameters can be measured in a later analytic step, e.g., where reactions are carried out in the separate reaction volumes and the reaction products are recovered and analyzed. In particular embodiments, one or more parameter measurements are carried out “in parallel,” i.e., essentially simultaneously in the separate reaction volumes.

    Abstract translation: 在某些实施方案中，本发明提供了用于测定颗粒群体中的单个颗粒的方法和装置，其中针对每个颗粒测量至少两个参数。 当颗粒处于分开的反应体积中时，可以测量一个或多个参数。 或者或另外，可以在稍后的分析步骤中测量一个或多个参数，例如其中在分开的反应体积中进行反应并回收和分析反应产物。 在具体实施方案中，一个或多个参数测量“并行地进行”，即基本上同时在单独的反应体积中进行。

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