公开(公告)号：EP2569453A2

公开(公告)日：2013-03-20

申请号：EP11781412.9

申请日：2011-05-16

Applicant: Fluidigm Corporation

Inventor： MAY, Andrew ,  MIR, Alain ,  RAMAKRISHNAN, Ramesh ,  ZIMMERMANN, Bernhard

IPC: C12Q1/68 ,  C12N15/10

CPC classification number: C12P19/34 ,  C07H21/00 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q2600/118 ,  C12Q2565/113 ,  C12Q2527/125 ,  C12Q2525/204 ,  C12Q2525/307 ,  C12Q2525/15 ,  C12Q2521/507

Abstract: The present invention provides methods for selectively enriching a biological sample for short nucleic acids, such as fetal DNA in a maternal sample or apoptic DNA in a biological sample from a cancer patient and for subsequently analyzing the short nucleic acids for genotype, mutation, and/or aneuploidy.

公开(公告)号：EP3285062B1

公开(公告)日：2019-08-07

申请号：EP17180284.6

申请日：2013-02-28

Applicant: Fluidigm Corporation

Inventor： FOWLER, Brian ,  KIMBALL, Jake ,  MAUNG, Myo Thu ,  MAY, Andrew ,  NORRIS, Michael C. ,  TOPPANI, Dominique G. ,  UNGER, Marc A. ,  WANG, Jing ,  WEST, Jason A. A.

IPC: G01N15/10

公开(公告)号：EP2820394A1

公开(公告)日：2015-01-07

申请号：EP13754093.6

申请日：2013-02-28

Applicant: Fluidigm Corporation

Inventor： FOWLER, Brian ,  KIMBALL, Jake ,  MAUNG, Myo Thu ,  MAY, Andrew ,  NORRIS, Michael C. ,  TOPPANI, Dominique G. ,  UNGER, Marc A. ,  WANG, Jing ,  WEST, Jason A. A.

IPC: G01N15/10

CPC classification number: G01N1/28 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0668 ,  B01L2300/0864 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0487 ,  B01L2400/086 ,  B01L2400/088 ,  C12P19/34 ,  C12Q1/6813 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q1/6869 ,  G01N1/34 ,  G01N15/1484 ,  C12Q2565/629

Abstract: Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing. Reaction products may be harvested and/or further analyzed in some cases.

公开(公告)号：EP2710172A2

公开(公告)日：2014-03-26

申请号：EP12789957.3

申请日：2012-05-21

Applicant: Fluidigm Corporation

Inventor： ANDERSON, Megan ,  CHEN, Peilin ,  FOWLER, Brian ,  JONES, Robert C. ,  KAPER, Fiona ,  LEBOFSKY, Ronald ,  MAY, Andrew

IPC: C40B20/00 ,  C40B50/06 ,  C40B40/06 ,  C40B30/04 ,  C12P19/34 ,  C12Q1/68

CPC classification number: C12Q1/6855 ,  C12N15/10 ,  C12N15/1065 ,  C12N15/65 ,  C12N15/66 ,  C12Q1/686 ,  C12Q1/6874

Abstract: Described herein are methods useful for incorporating one or more adaptors and/or nucleotide tag(s) and/or barcode nucleotide sequence(s) one, or typically more, target nucleotide sequences. In particular embodiments, nucleic acid fragments having adaptors, e.g., suitable for use in high-throughput DNA sequencing are generated. In other embodiments, information about a reaction mixture is encoded into a reaction product. Also described herein are methods and kits useful for amplifying one or more target nucleic acids in preparation for applications such as bidirectional nucleic acid sequencing. In particular embodiments, methods of the invention entail additionally carrying out bidirectional DNA sequencing. Also described herein are methods for encoding and detecting and/or quantifying alleles by primer extension.

Abstract translation: 本文描述的方法可用于掺入一个或多个衔接子和/或核苷酸标签和/或条形码核苷酸序列，一个或多个靶核苷酸序列。 在具体实施方案中，产生具有适配器（例如适合用于高通量DNA测序）的核酸片段。 在其它实施方案中，关于反应混合物的信息被编码成反应产物。 本文还描述了可用于扩增一种或多种靶核酸以准备应用如双向核酸测序的方法和试剂盒。 在具体实施方案中，本发明的方法还需要进行双向DNA测序。 本文还描述了通过引物延伸来编码和检测和/或定量等位基因的方法。

公开(公告)号：EP2414547B1

公开(公告)日：2014-03-12

申请号：EP10759511.8

申请日：2010-04-02

Applicant: Fluidigm Corporation

Inventor： MAY, Andrew ,  CHEN, Peilin ,  WANG, Jun ,  KAPER, Fiona ,  ANDERSON, Megan

IPC: C12Q1/68

CPC classification number: C12Q1/6806 ,  B01L3/50273 ,  B01L3/502738 ,  B01L7/52 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2400/0487 ,  B01L2400/0655 ,  C12Q1/686 ,  C12Q2525/155 ,  C12Q2525/161 ,  C12Q2527/143 ,  C12Q2535/122 ,  C12Q2549/119 ,  C12Q2563/179 ,  C12Q2565/629

公开(公告)号：EP1938101A2

公开(公告)日：2008-07-02

申请号：EP06803816.5

申请日：2006-09-13

Applicant: Fluidigm Corporation

Inventor： NASSEF, Hany, Ramez ,  CHOU, Hou-pu ,  LUCERO, Michael ,  MAY, Andrew ,  YOKOBATA, Kathy

IPC: G01N33/543 ,  B01L3/00

CPC classification number: G01N33/54366 ,  B01J2219/00286 ,  B01J2219/00317 ,  B01J2219/00389 ,  B01J2219/00398 ,  B01L3/5025 ,  B01L3/502715 ,  B01L3/502738 ,  B01L2300/0816 ,  B01L2300/0874 ,  B01L2300/123 ,  B01L2400/0487 ,  B01L2400/0655 ,  F16K99/0001 ,  F16K99/0015 ,  F16K99/0051 ,  F16K99/0059 ,  F16K2099/0074 ,  F16K2099/008 ,  F16K2099/0084 ,  G01N33/5302

Abstract: A microfluidic device adapted to perform many simultaneous binding assays including but not limited to immunological experiments, such as ELISA assays, with minimal cross-talk between primary and secondary antibodies.