公开(公告)号：JP2007278985A

公开(公告)日：2007-10-25

申请号：JP2006108855

申请日：2006-04-11

Applicant: Hamamatsu Photonics Kk ,  浜松ホトニクス株式会社

Inventor： KATAOKA TAKUJI ,  MATSUBARA MASANORI

IPC: G01N21/64

Abstract: PROBLEM TO BE SOLVED: To provide a light measuring device, light measuring method, and to provide a light measuring program capable of improving the measuring sensitivity with respect to light from a sample. SOLUTION: A fluorescence measuring apparatus 1 for measuring light from the sample held in a well of a microplate 20 comprises an image acquiring section 40 for acquiring a two-dimensional photodetection image, and a data analysis device 50 for analyzing measuring data, including the photodetection image. The data analysis device 50 extracts an analysis region to be analyzed from the measurement region set, in response to the well on the photodetection image, by comparing two-dimensional light intensity distribution in the measurement region with a predetermined light intensity threshold, and performs analysis processings of the sample by using the data of the light intensity inside the analysis region as the analysis data. COPYRIGHT: (C)2008,JPO&INPIT

Abstract translation: 要解决的问题：提供一种光测量装置，光测量方法，并且提供能够提高相对于来自样品的光的测量灵敏度的光测量程序。 解决方案：用于测量保存在微板20的孔中的样品的光的荧光测量装置1包括用于获取二维光电图像的图像获取部分40和用于分析测量数据的数据分析装置50， 包括光电检测图像。 数据分析装置50通过将测量区域中的二维光强度分布与预定的光强度阈值进行比较，通过对光检测图像上的阱进行比较，从测量区域集中提取要分析的分析区域，并进行分析 通过使用分析区域内的光强度的数据作为分析数据来处理样品。 版权所有（C）2008，JPO＆INPIT

公开(公告)号：JP2007256203A

公开(公告)日：2007-10-04

申请号：JP2006083822

申请日：2006-03-24

Applicant: Asahi Breweries Ltd ,  Hamamatsu Photonics Kk ,  National Agriculture & Food Research Organization ,  アサヒビール株式会社 ,  浜松ホトニクス株式会社 ,  独立行政法人農業・食品産業技術総合研究機構

Inventor： HINO AKIHIRO ,  KUSAKABE HIROKO ,  MIZUGUCHI YOSHINORI ,  OZEKI HIDEO ,  KOIKE KAZUYUKI ,  KATAOKA TAKUJI ,  SHINDO YOICHIRO

IPC: G01N1/00 ,  G01N37/00

Abstract: PROBLEM TO BE SOLVED: To provide an analyzer for measuring the response of a cell, with respect to a sample.
SOLUTION: This cell response analyzer 100 is used for measuring the response of the cell 14 with respect to the sample 60a. The analyzer 100 is provided with a flow passage 12 that immobilizes the cell 14, a reservoir part 20 for temporarily retaining the sample 60a and a buffer solution 40a supplied to the flow passage 12, and a measuring instrument 30 for measuring the response of the cell 14, with respect to the sample 60a.
COPYRIGHT: (C)2008,JPO&INPIT

Abstract translation: 要解决的问题：提供一种用于测量细胞相对于样品的响应的分析器。 解决方案：该电池响应分析器100用于测量电池14相对于样品60a的响应。 分析装置100设置有固定电池单元14的流路12，用于临时保持试样60a的储存部20和供给流路12的缓冲溶液40a，以及测定电池的响应的测定装置30 如图14所示，相对于样品60a。 版权所有（C）2008，JPO＆INPIT

公开(公告)号：JP2007298380A

公开(公告)日：2007-11-15

申请号：JP2006126349

申请日：2006-04-28

Applicant: Hamamatsu Photonics Kk ,  浜松ホトニクス株式会社

Inventor： KATAOKA TAKUJI ,  MATSUBARA MASANORI

IPC: G01N21/03 ,  G01N37/00

Abstract: PROBLEM TO BE SOLVED: To provide a sample holding member which enables the efficient measurement of emission under various conditions such as the measurement of multicolor emission or the like.
SOLUTION: The sample holding member 2 is constituted of a microplate 20 having a plurality of wells 21, which respectively hold a sample, arranged thereto in a two-dimensional array state and the filter member 30 provided so as to be fixed at a predetermined position where the emission from the sample held in each of the wells 21 of the microplate 20 transmits. Further, the filter member 30 has a plurality of optical filter parts 31 arranged in a two-dimensional array state so as to correspond to a plurality of the wells 21 of the microplate 20 and a plurality of the optical filter parts 31 respectively have light transmission characteristics with respect to the wavelength corresponding to a wavelength selectivity necessary for measuring emission.
COPYRIGHT: (C)2008,JPO&INPIT

Abstract translation: 要解决的问题：提供一种能够在诸如测量多色发射等的各种条件下有效测量发射的样品保持构件。 解决方案：样品保持构件2由微孔板20构成，微孔板20具有分别保持样品的多个孔21，其以二维排列状态布置在其上，并且过滤构件30设置成固定在 来自保存在微板20的各个孔21中的来自样品的发射的预定位置发送。 此外，过滤器构件30具有多个排列成二维排列状态的滤光器部件31，以与微板20的多个孔21对应，并且多个滤光器部件31分别具有透光性 相对于对应于测量发射所需的波长选择性的波长的特性。 版权所有（C）2008，JPO＆INPIT

公开(公告)号：JP2007278984A

公开(公告)日：2007-10-25

申请号：JP2006108838

申请日：2006-04-11

Applicant: Hamamatsu Photonics Kk ,  浜松ホトニクス株式会社

Inventor： KATAOKA TAKUJI ,  MATSUBARA MASANORI

IPC: G01N21/64 ,  G01J1/42 ,  G01N21/03

Abstract: PROBLEM TO BE SOLVED: To provide a light measuring device, light measurement method, and light measuring program capable of suppressing the influence due to dust or the like. SOLUTION: A fluorescence measurement apparatus 1, for measuring fluorescence from a sample in a held state by a sample holding member such as a microplate 20, comprises an image acquiring section 40 for acquiring two-dimensional fluorescence images by fluorescence emitted from the sample, and a data analysis device 50 for performing analysis processings of measured data, including the fluorescence image. The fluorescence measuring apparatus extracts a measurement excluding region from the measurement region set for the fluorescence image, by comparing the light intensity distribution in the measurement region of a reference image, acquired under predetermined conditions with a light intensity threshold, that is sets as an analysis region, the region obtained by excluding the measurement excluding region from the measurement region, and performs analysis processings of the fluorescence image. COPYRIGHT: (C)2008,JPO&INPIT

Abstract translation: 要解决的问题：提供能够抑制灰尘等的影响的光测量装置，光测量方法和光测量程序。 解决方案：一种荧光测量装置1，用于通过诸如微板20的样品保持部件测量来自保持状态的样品的荧光，包括图像获取部40，用于通过从所述微板20发射的荧光获取二维荧光图像 样本和用于对包括荧光图像在内的测量数据进行分析处理的数据分析装置50。 荧光测量装置通过将在预定条件下获取的参考图像的测量区域中的光强度分布与被设置为分析的光强度阈值进行比较，从针对荧光图像设置的测量区域中提取测量排除区域 区域，通过从测量区域排除测量排除区域而获得的区域，并且执行荧光图像的分析处理。 版权所有（C）2008，JPO＆INPIT

公开(公告)号：JP2003093041A

公开(公告)日：2003-04-02

申请号：JP2001291874

申请日：2001-09-25

Applicant: HAMAMATSU PHOTONICS KK

Inventor： KATAOKA TAKUJI ,  MIZUGUCHI YOSHINORI

IPC: G01N21/64 ,  C12M1/34

Abstract: PROBLEM TO BE SOLVED: To provide a cultured specimen-observing device capable of taking a picture without taking out the cultured specimen from a culturing case. SOLUTION: This cultured specimen-observing device for culturing the bio- specimens housed in multiple culturing instruments 13 in a culturing case 3 and taking pictures is equipped with multiple specimen stands 6 for arranging the multiple culturing instruments 13 and installed in the culturing case 3, a picture-taking unit 14 installed in the culturing case 3, taking the pictures of the culturing instruments 13 and displaying them at the outside of the culturing case 3, transporting means 10, 29 for transporting the picture-taking unit 14 and culturing instruments 13 relatively so as to make an arrangement capable of taking the picture of a desired culturing instrument 13 with the picture-taking unit 14 and a controlling means for controlling the transporting means 10, 29. Thereby, it becomes possible to take the picture of the desired culturing instrument 13 in the cultured case 3 by using the picture-taking unit, and to observe the desired culturing instrument 13 from the outside of the culturing case 3.

公开(公告)号：JP2003093040A

公开(公告)日：2003-04-02

申请号：JP2001291965

申请日：2001-09-25

Applicant: HAMAMATSU PHOTONICS KK

Inventor： KATAOKA TAKUJI ,  MIZUGUCHI YOSHINORI

IPC: C12M1/00 ,  C12Q1/02

Abstract: PROBLEM TO BE SOLVED: To provide a bio-specimen-observing device and a method therefor, capable of sufficiently preventing the cultured bio-specimen from undesirable effects in a clean bench. SOLUTION: The method for observing the bio-specimen comprises a process of culturing bio-specimens contained in multiple number of containers 17 in a cultivator 3, a process of transporting the containers 17 from the cultivator 3 to the clean bench 2, and a process of observing the bio-specimens contained in the containers 17 by using a microscope 16 installed in the clean bench 2, and also comprises a process of surrounding the containers 17 with a culturing case 46 in the clean bench 2 and a process of adjusting the temperature, humidity and carbon dioxide concentration in a space formed between the culturing case 46 and the containers 17. In this case, it is possible to make the temperature, humidity and carbon dioxide concentration in the space formed between the culturing case 46 and the containers 17 same as those in the cultivator 3, and therefore, it becomes possible to observe the bio-specimen contained in the containers 17 in the same state as in the cultivator 3.

公开(公告)号：JP2014153154A

公开(公告)日：2014-08-25

申请号：JP2013022234

申请日：2013-02-07

Applicant: Hamamatsu Photonics Kk ,  浜松ホトニクス株式会社

Inventor： IWAI HIDENAO ,  KATAOKA TAKUJI ,  YAMAMOTO SATOSHI ,  SUGIYAMA NORIKAZU ,  YAMAUCHI TOYOHIKO ,  GOTO KENTARO

IPC: G01N21/47 ,  G01B11/00 ,  G01N21/17 ,  G01P3/36

CPC classification number: G01N33/4833 ,  G01B11/00 ,  G01N21/47 ,  G01N21/49 ,  G01N2021/4707 ,  G01N2021/479

Abstract: PROBLEM TO BE SOLVED: To provide a method and device capable of easily evaluating motion of aggregated cells.SOLUTION: An aggregated cells evaluation device 1 includes a laser beam source 10, a speckle image acquisition part 20, an SC calculation part 30, an evaluation part 40, and a storage part 50. The speckle image acquisition part 20 acquires a two-dimensional speckle image caused by forward scattered light generated in aggregated cells 90 by irradiation of the aggregated cells 90 with laser beam output from the laser beam source 10. The SC calculation part 30 calculates a speckle contrast value Kof a speckle image Iat each time tto obtain a maximum value Kamong speckle contrast values Kto Kand normalizes the speckle contrast value Kat each time tby the maximum value Kto obtain a normalized speckle contrast value K'. The evaluation part 40 evaluates motion of the aggregated cells 90 on the basis of the normalized speckle contrast value K' at each time t.

Abstract translation: 要解决的问题：提供能够容易地评估聚集电池的运动的方法和装置。解决方案：聚集电池评估装置1包括激光束源10，散斑图像获取部20，SC计算部30，评价 部分40和存储部分50.斑点图像获取部分20通过从激光束源10输出的激光束照射聚集单元90，获得由聚集单元90中产生的正向散射光引起的二维散斑图像。 SC计算部30计算每次得到最大值Kamong散斑对比度值Kto K的斑点图像Iat的散斑对比度值K，并且每次以最大值K为标准化散斑对比度值即可得到归一化斑点对比度值K'。 评价部40基于每个时刻t的归一化斑点对比度值K'来评价聚集单元90的运动。

公开(公告)号：JP2013101160A

公开(公告)日：2013-05-23

申请号：JP2013043343

申请日：2013-03-05

Applicant: Hamamatsu Photonics Kk ,  浜松ホトニクス株式会社

Inventor： KATAOKA TAKUJI ,  ITO HIRAYOSHI

IPC: G01N21/64

Abstract: PROBLEM TO BE SOLVED: To provide a light measurement device for reducing a background light noise from a micro-plate.SOLUTION: Disclosed is a light irradiation device for irradiating a micro-plate 20 provided with a plurality of wells for storing a measurement object with irradiation rays of light and a light measurement device including the light irradiation device. The light measurement device includes: a light guide member 61 having a main face 61a on which a plurality of projecting parts 61e with almost the same shapes are formed, a back face 61h being a face which is an opposite side of the main face 61a, and side faces 61b which are almost orthogonal to the main face 61a; and a light source device 62 for making irradiation rays of light 61b incident from the side faces 61b of the light guide member 61 to the light guide member 61. The projecting part 61e has an upper face 61f which is almost in parallel with the main face 61a, and the light guide member 61 can be arranged such that an upper face 61f of the projecting part 61e is brought into contact with a back face 23 of the micro-plate 20. Also, the light guide member 61 can be arranged such that the upper face 61f of the projecting part 61e faces the bottom face of a well 21 of the micro-plate 20.

Abstract translation: 要解决的问题：提供一种用于减小微板背景光噪声的光测量装置。 解决方案：公开了一种光照射装置，用于照射具有多个用于存储照射光线的测量对象的孔的微板20和包括该光照射装置的光测量装置。 光测量装置包括：导光构件61，其具有形成有几乎相同形状的多个突出部61e的主面61a，作为主面61a的相反侧的面的背面61h， 以及与主面61a大致正交的侧面61b; 以及用于使光61b的照射光从导光构件61的侧面61b入射到导光构件61的光源装置62.突出部61e具有与主面大致平行的上面61f 61a和导光构件61可以布置成使得突出部分61e的上表面61f与微板20的背面23接触。另外，导光构件61可以布置成使得 突出部61e的上表面61f面对微板20的孔21的底面。版权所有（C）2013，JPO＆INPIT

公开(公告)号：JP2013003091A

公开(公告)日：2013-01-07

申请号：JP2011137314

申请日：2011-06-21

Applicant: Hamamatsu Photonics Kk ,  浜松ホトニクス株式会社

Inventor： KATAOKA TAKUJI ,  MATSUBARA MASANORI

IPC: G01N21/64 ,  G01N33/48 ,  G01N33/483

CPC classification number: G02B21/365 ,  G01N21/6452 ,  G01N21/6458 ,  G06T7/0016 ,  G06T2207/10016 ,  G06T2207/10056 ,  G06T2207/30024 ,  G06T2207/30242

Abstract: PROBLEM TO BE SOLVED: To specify a pixel whose luminance value chronologically changes, in an image containing light emitted from a sample.SOLUTION: An optical measurement device 1 is used for measuring light from a sample and includes: a motion image obtainment part 40 for obtaining motion image data of a two-dimensional light image containing light from a sample; and an analysis process part 51 for analyzing the motion image data. The analysis process part 51 includes: a luminance value data obtainment part 52a for obtaining luminance value data indicating chronological changes of a luminance value in plural pixels constituting a zone corresponding to plural wells; a luminance value extraction part 52b for extracting a peak value of a luminance value and a bottom value thereof from the luminance value data; and a pixel specifying part 52c for calculating an evaluation value evaluating a state of change in the luminance value on the basis of the peak and bottom values, and specifying a target pixel, being a target of analysis, from among the plural pixels on the basis of repetition of the evaluation value.

Abstract translation: 要解决的问题：在包含从样品发射的光的图像中指定其亮度值按时间顺序改变的像素。 解决方案：光学测量装置1用于测量来自样品的光，并包括：运动图像获取部分40，用于获得包含来自样本的光的二维光图像的运动图像数据; 以及用于分析运动图像数据的分析处理部分51。 分析处理部分51包括：亮度值数据获取部分52a，用于获得指示构成对应于多个井的区域的多个像素的亮度值的时间变化的亮度值数据; 亮度值提取部52b，用于从亮度值数据中提取亮度值的峰值及其底值; 以及像素指定部52c，用于基于峰值和下降值来计算评价亮度变化状况的评价值，并且从基准的多个像素中指定作为分析对象的目标像素 重复评估值。 版权所有（C）2013，JPO＆INPIT

公开(公告)号：JP2007298379A

公开(公告)日：2007-11-15

申请号：JP2006126346

申请日：2006-04-28

Applicant: Hamamatsu Photonics Kk ,  浜松ホトニクス株式会社

Inventor： KATAOKA TAKUJI ,  MATSUBARA MASANORI

IPC: G01N21/76 ,  G01N21/78

Abstract: PROBLEM TO BE SOLVED: To provide a device for measuring light emission capable of efficiently performing the measurement of multicolor emission, and to provide a method of measuring the light emission.
SOLUTION: The device 1 for measuring the emission from the sample held in each of the wells of a microplate 20 includes an image acquiring part 40 for acquiring a two-dimensional emission image, a light guide optical system 41 for guiding the two-dimensional image of the microplate 20 containing the emission from the sample to the image acquirig part 40 and the sheetlike filter member 30 arranged on a light path of the two-dimensional image. In the filter member 30, a plurality of optical filter parts are arranged in a two-dimensional array state so as to correspond to a plurality of the wells of the microplate 20 and constituted of a plurality of filter regions mutually different in light transmission characteristics with respect to a wavelength.
COPYRIGHT: (C)2008,JPO&INPIT

Abstract translation: 要解决的问题：提供一种用于测量能够有效执行多色发射测量的发光的装置，并提供一种测量发光的方法。 解决方案：用于测量保存在微板20的每个孔中的样品的发射的装置1包括用于获取二维发射图像的图像获取部40，用于引导二维发射图像的导光光学系统41 包含从样品发射到图像取出部分40的微板20的三维图像和布置在二维图像的光路上的片状过滤元件30。 在过滤构件30中，多个滤光器部件被布置成二维阵列状态，以对应于微板20的多个孔，并且由光传输特性互不相同的多个滤光器区域构成， 尊重波长。 版权所有（C）2008，JPO＆INPIT