公开(公告)号：JP2012001462A

公开(公告)日：2012-01-05

申请号：JP2010136376

申请日：2010-06-15

Applicant: Kaneka Corp ,  株式会社カネカ

Inventor： OKUBO TAKAHIRO ,  SHIBATA TAKAO ,  HONDA TATSUYA

IPC: C07K14/31 ,  C07K1/22 ,  C07K16/00 ,  C07K17/14

Abstract: PROBLEM TO BE SOLVED: To provide a column with which an antibody is efficiently purified, and which is simply and conveniently produced.SOLUTION: There are disclosed: an inorganic monolith to which a protein (for example, protein A) having antibody binding affinity is fixed; a method for producing an inorganic monolith with a protein fixed thereto via an amino group or a thiol group, by bringing an inorganic monolith, with an epoxy group introduced thereinto, into contact with a solution of the protein; an adsorbing body composed of the inorganic monolith; and an affinity chromatograph for antibody purification, which is equipped with the adsorbing body and which has high adsorption performance at high linear velocity.

Abstract translation: 待解决的问题：提供一种抗体有效纯化的柱，其简单而方便地制备。 解决方案：公开了固定具有抗体结合亲和力的蛋白质（例如蛋白A）的无机整料; 通过使其中引入了环氧基的无机整料与蛋白质溶液接触来制备具有通过氨基或硫醇基固定的蛋白质的无机整料的方法; 由无机整料组成的吸附体; 和用于抗体纯化的亲和色谱仪，其配备有吸附体，并且在高线速度下具有高吸附性能。 版权所有（C）2012，JPO＆INPIT

公开(公告)号：JP2011252723A

公开(公告)日：2011-12-15

申请号：JP2010125092

申请日：2010-05-31

Applicant: Kaneka Corp ,  Shimadzu Corp ,  株式会社カネカ ,  株式会社島津製作所

Inventor： OKUBO TAKAHIRO ,  TONE KOJI ,  SHIBATA TAKAO ,  HORIIKE SHIGEYOSHI ,  WATABE YOSHIYUKI

IPC: G01N30/88 ,  B01J20/26

Abstract: PROBLEM TO BE SOLVED: To provide an organic polymer monolith separation medium into which a long chain acyl group with excellent reverse-phase separation property has been introduced.SOLUTION: The separation medium is constituted of an organic polymer monolith having an 8-24C acyl group. The organic polymer monolith preferably has at least one group selected from the following formula 1, formula 2, and formula 3, and the average carbon number of the acyl group is, for example, 16-20. -C(OR)-C(OH)-...(1), -C(OR)-C(OR)-...(2), -C(OR)-C(A)-...(3) (In the formulas, Rand Rrepresent 8-24C acyl group same as or different from each other. A represents an ammonia residue or a primary amine residue.)

Abstract translation: 待解决的问题：提供一种有机聚合物整料分离介质，其中引入了具有优异反相分离性能的长链酰基。 解决方案：分离介质由具有8-24C酰基的有机聚合物整料构成。 有机聚合物整料优选具有选自下式1，式2和式3中的至少一个基团，酰基的平均碳数为例如16-20。 -C（OR 1 ）-C（OH）-...（1），-C（OR 1 ） - C（OR 2 ）-...（2），-C（OR 1 ） - C（A） （3）（式中，R 1 和R 2 表示与之相同或不同的8-24C酰基 A代表氨残基或伯胺残基。）版权所有（C）2012，JPO＆INPIT