公开(公告)号：EP3254083A1

公开(公告)日：2017-12-13

申请号：EP16708006.8

申请日：2016-02-05

Applicant: Life Technologies Corporation

Inventor： CHU, Yong ,  FREUDENTHAL, Jacob ,  MARKS, Jeffrey

IPC: G01N21/27 ,  G01N21/64 ,  C12Q1/68

CPC classification number: C12Q1/686 ,  G01N21/274 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/6456 ,  G01N2021/6421 ,  G01N2021/6441 ,  G01N2201/127

Abstract: Some embodiments describe a computer-implemented method for calibrating a fluorescent dye. The method can comprise imaging a sample holder, loaded into an instrument, at more than one channel. The sample holder can comprise a plurality of reaction sites and more than one dye type, with each dye occupying more than one reaction site. The method can further comprise identifying a peak channel for each dye on the sample holder, normalizing each channel to the peak channel for each dye, and producing a dye matrix that can comprise a set of dye reference values.

公开(公告)号：EP3254079A2

公开(公告)日：2017-12-13

申请号：EP16705012.9

申请日：2016-02-05

Applicant: Life Technologies Corporation

Inventor： CHU, Yong ,  MARKS, Jeffrey ,  FREUDENTHAL, Jacob ,  WESSEL, Thomas ,  WOO, David

IPC: G01N21/27 ,  G01N21/64 ,  C12Q1/68

CPC classification number: G01N21/6428 ,  C12Q1/6851 ,  C12Q1/686 ,  G01N21/274 ,  G01N21/278 ,  G01N21/6452 ,  G01N21/6456 ,  G01N2021/6439 ,  G01N2021/6471 ,  G01N2201/127 ,  G01N2201/13 ,  G01N2333/922

Abstract: In one exemplary embodiment, a method for calibrating an instrument is provided. The instrument includes an optical system capable of imaging florescence emission from a plurality of reaction sites. The method includes performing a region-of-interest (ROI) calibration to determine reaction site positions in an image. The method further includes performing a pure dye calibration to determine the contribution of a fluorescent dye used in each reaction site by comparing a raw spectrum of the fluorescent dye to a pure spectrum calibration data of the fluorescent dye. The method further includes performing an instrument normalization calibration to determine a filter normalization factor. The method includes performing an RNase P validation to validate the instrument is capable of distinguishing between two different quantities of sample.

公开(公告)号：EP2761277A1

公开(公告)日：2014-08-06

申请号：EP12783710.2

申请日：2012-09-28

Applicant: Life Technologies Corporation

Inventor： MAHER, Kevin ,  CHEN, Mingsong ,  FREUDENTHAL, Jacob ,  BOO, Kuan, Moon ,  LAU, Soo, Yong ,  FORTESCUE, David ,  SHEN, Ming ,  SOH, Woon Liang, Terence

IPC: G01N21/64

CPC classification number: G01N21/6486 ,  G01N21/6428 ,  G01N21/6452 ,  G01N2021/6471 ,  G01N2021/6478 ,  G01N2201/062

Abstract: An instrument for processing and/or measuring a biological process contains an excitation source, a sample holder, an optical sensor, an excitation optical system, and an emission optical system. The sample holder is configured to receive a plurality of biological samples. The optical sensor is configured to receive an emission from the biological samples. The excitation optical system is disposed along an excitation optical path and is configured to direct the electromagnetic radiation from the excitation source to the biological samples. The emission optical system is disposed along an emission optical path and is configured to direct electromagnetic emissions from the biological samples to the optical sensor. The instrument further contains a plurality of filter assemblies configured to be interchangeably located along at least one of the optical paths. The plurality of filter components includes a first filter assembly characterized by a first optical power and a first filter having a first filter function, the first filter function characterized by at least one of a first low-pass wavelength or a first high-pass wavelength. The second filter assembly is characterized by a second optical power and a second filter having a second filter function, the second filter function comprising at least one of a second low-pass wavelength that is different than the first low-pass wavelength or a second high-pass wavelength that is different than the first high-pass wavelength.

Abstract translation: 用于处理和/或测量生物过程的仪器包含激发源，样品架，光学传感器，激发光学系统和发射光学系统。 样品架配置成接收多个生物样品。 光学传感器被配置为接收来自生物样品的发射。 激发光学系统沿着激发光学路径设置并且被配置为将来自激发源的电磁辐射引导到生物样品。 发射光学系统沿着发射光路设置并且被配置为将来自生物样品的电磁辐射引导到光学传感器。 仪器还包括多个过滤器组件，其被配置为沿着至少一个光路可互换地定位。 多个滤波器部件包括第一滤波器组件，其特征在于第一光功率和具有第一滤波器功能的第一滤波器，第一滤波器功能的特征在于第一低通波长或第一高通波长中的至少一个。 第二滤波器组件的特征在于具有第二滤波器功能的第二光功率和第二滤波器，第二滤波器功能包括与第一低通波长不同的第二低通波长或第二高通波长中的至少一个 通过波长不同于第一高通波长。