公开(公告)号：EP3228715B1

公开(公告)日：2019-08-14

申请号：EP17160771.6

申请日：2013-02-08

Applicant: Life Technologies Corporation

Inventor： MENCHEN, Steven ,  BLANCHARD, Alan ,  ANDRUZZI, Luisa ,  KHAN, Shaheer ,  GREMYACHINSKIY, Dmitriy ,  LUI, Alfred ,  STOLARCZYK, Craig ,  SOKOLSKY, Tanya

IPC: C12Q1/68

公开(公告)号：EP2812446A1

公开(公告)日：2014-12-17

申请号：EP13705357.5

申请日：2013-02-08

Applicant: Life Technologies Corporation

Inventor： MENCHEN, Steven ,  BLANCHARD, Alan ,  ANDRUZZI, Luisa ,  KHAN, Shaheer ,  GREMYACHINSKIY, Dmitriy ,  LUI, Alfred ,  STOLARCZYK, Craig ,  SOKOLSKY, Tanya

IPC: C12Q1/68

CPC classification number: C12Q1/6876 ,  C08F8/40 ,  C12Q1/6834 ,  C12Q2523/101 ,  C12Q2563/149 ,  C12Q2565/119

Abstract: A method of conjugating a substrate includes exchanging a counter ion associated with a biomolecule with a lipophilic counter ion to form a biomolecule complex, dispersing the biomolecule complex in a nonaqueous solvent, and coupling the biomolecule complex to a substrate in the presence of the nonaqueous solvent. The substrate or solid support is a polymeric particle, the biomolecule to be immobilized on the particle is an oligonucleotide, the lipophilic counter ion is a tetrabutylammonium ion and the nonaqueous solvent is N- methylpyrrolidone (NMP). The oligonucleotide coated particles are used in nucleic acid isolation or in nucleic acid sequencing reactions.

公开(公告)号：EP2812360A2

公开(公告)日：2014-12-17

申请号：EP13706818.5

申请日：2013-02-08

Applicant: Life Technologies Corporation

Inventor： FONNUM, Geir ,  MODAHL, Grete ,  KJUS, Nini ,  MOLTEBERG, Astrid Evenroed ,  TRAN, Diem ,  AASERUD, Jo ,  GOKMEN, M. Talha ,  MENCHEN, Steven ,  FULLER, Carl ,  ANDRUZZI, Luisa ,  HINZ, Wolfgang ,  GREMYACHINSKIY, Dmitriy ,  LIGHT, David ,  MASTROIANNI, Alexander ,  ROSENBLUM, Barnett ,  KHAN, Shaheer ,  STOLARCZYK, Craig ,  SOKOLSKY, Tanya

IPC: C08F2/16 ,  C08F220/54 ,  C08F8/12 ,  C08F222/38 ,  C12Q1/68 ,  C08F2/20 ,  C08F2/26 ,  C08F230/08 ,  C08F257/02 ,  C08F220/58 ,  C08F220/60 ,  C08F8/34 ,  C08F130/08 ,  C07F7/18

CPC classification number: C08F130/08 ,  C07F7/1852 ,  C07F7/188 ,  C08F2/20 ,  C08F2/26 ,  C08F6/24 ,  C08F8/12 ,  C08F8/32 ,  C08F8/34 ,  C08F220/58 ,  C08F222/385 ,  C08F230/08 ,  C08F2800/20 ,  C08J9/20 ,  C08J2343/04 ,  C12P19/34 ,  C12Q1/686 ,  C12Q1/6874 ,  G01N27/4145 ,  Y10T428/2982 ,  C08F257/02 ,  C08F2222/1013 ,  C08F212/36 ,  C08F220/60 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2565/543

Abstract: A method of forming a particle includes, in a disperse phase within an aqueous suspension, polymerizing a plurality of mer units of a hydrophilic monomer having a hydrophobic protection group, thereby forming a polymeric particle including a plurality of the hydrophobic protection groups. The method further includes converting the polymeric particle to a hydrophilic particle.