公开(公告)号：EP3254080A1

公开(公告)日：2017-12-13

申请号：EP16706289.2

申请日：2016-02-05

Applicant: Life Technologies Corporation

Inventor： WESSEL, Thomas ,  CHU, Yong ,  FREUDENTHAL, Jacob ,  WOO, David

IPC: G01N21/27 ,  G01N21/64 ,  C12Q1/68 ,  G06F19/26

CPC classification number: G01N21/6428 ,  G01N21/274 ,  G01N21/6452 ,  G01N21/6456 ,  G01N2021/6439 ,  G06F19/24

Abstract: In one exemplary embodiment, a method for validating an instrument is provided. The method includes receiving amplification data from a validation plate to generate a plurality of amplification curves. The validation plate includes a sample of a first quantity and a second quantity, and each amplification curve includes an exponential region. The method further includes determining a set of fluorescence thresholds based on the exponential regions of the plurality of amplification curves and determining, for each fluorescence threshold of the set, a first set of cycle threshold (Ct) values of amplification curves generated from the samples of the first quantity and a second set of Ct values of amplification curves generated from the samples of the second quantity. The method includes calculating if the first and second quantities are sufficiently distinguishable based on Ct values at each of the plurality of fluorescence thresholds.

公开(公告)号：EP2895978A1

公开(公告)日：2015-07-22

申请号：EP13773921.5

申请日：2013-09-13

Applicant: Life Technologies Corporation

Inventor： MAJUMDAR, Nivedita Sumi ,  WESSEL, Thomas ,  WOO, David C. ,  SIKORA, Marcin ,  JANAWAY, Gordon ,  RAIZADA, Shweta ,  LANKESTER, Joanna ,  MARKS, Jeffrey ,  WESSEL, Daniel

IPC: G06F19/24

CPC classification number: G06F19/20 ,  C12Q1/686 ,  G06F19/24

Abstract: A computer-implemented method for designing a digital PCR (dPCR) experiment is provided. The method includes receiving, from a user, a selection of optimization type. The optimization type may be maximizing the dynamic range, minimizing the number of substrates including reaction sites needed for the experiment, determining a dilution factor, or determining the lower limit of detection, for example. The method further includes receiving, from the user, a precision measure for an experiment, and a minimum concentration of a target in a reaction site for the experiment. The method also includes determining a set of dPCR experiment design factors for the experiment based on the optimization type. The set of dPCR experiment design factors is then displayed to the user.

公开(公告)号：EP2780853A2

公开(公告)日：2014-09-24

申请号：EP12798036.5

申请日：2012-09-28

Applicant: Life Technologies Corporation

Inventor： WEISS, Howard ,  VAN HELEN, Eric ,  CHO, Jamie ,  WESSEL, Thomas ,  MORRISON, Thomas B.

IPC: G06F19/24

CPC classification number: C12Q1/6851 ,  G06F19/24 ,  G06F19/26

Abstract: According to one exemplary embodiment, a method for providing a amplification quality metric to a user is provided. The method includes receiving amplification data from an amplification of a sample to generate an amplification curve. The amplification curve includes an exponential region and a transition region. The method further includes determining a first value of the transition region and determining a second value of the transition region. The first value is the beginning of the transition region and the second value is the end of the transition region. Next, the amplification quality metric is calculated based on at least the first value and the second value. Then, the amplification quality metric is displayed to the user.

公开(公告)号：EP2761585A1

公开(公告)日：2014-08-06

申请号：EP12784376.1

申请日：2012-09-30

Applicant: Life Technologies Corporation

Inventor： FREUDENTHAL, Jacob K. ,  MARKS, Jeffrey A. ,  CHENG, Francis T. ,  WESSEL, Thomas

IPC: G06T5/00 ,  G06T5/50

CPC classification number: G06T7/0012 ,  G06T5/008 ,  G06T5/50 ,  G06T2207/10024 ,  G06T2207/10064 ,  G06T2207/10144 ,  G06T2207/20021 ,  G06T2207/20216 ,  G06T2207/30072

Abstract: A method for calibrating a biological instrument is provided. The method comprises the steps of acquiring an image of at least one biological sample array, determining a first region of interest within the image, wherein the first region of interest comprises a first plurality of locations on the at least one biological array; and identifying within the first region of interest, a plurality of image elements associated with each of the first plurality of locations on the at least one biological array.

Abstract translation: 提供了一种用于校准生物仪器的方法。 该方法包括以下步骤：获取至少一个生物样本阵列的图像，确定图像内的第一感兴趣区域，其中所述第一感兴趣区域包括所述至少一个生物阵列上的第一多个位置; 以及在所述第一感兴趣区域内识别与所述至少一个生物阵列上的所述第一多个位置中的每一个相关联的多个图像元素。