公开(公告)号：JP2006246491A

公开(公告)日：2006-09-14

申请号：JP2006065589

申请日：2006-03-10

Applicant: Olympus Corp ,  オリンパス株式会社

Inventor： KOBAYASHI SHIGERU ,  MATSUO YUICHIRO

IPC: H04N5/232 ,  G01N21/84 ,  G02B21/06 ,  G02B23/24 ,  H04N5/225 ,  H04N7/18

Abstract: PROBLEM TO BE SOLVED: To provide a video microscope in which image data for one frame are repeatedly read from a memory and displayed on a display device as a still picture and light source feeding power is controlled to extinct a light source during this display period.
SOLUTION: In the video microscope, an image of an observation object illuminated with illumination light generated by a light source 10 is captured via an optical magnification system 3, converted into a video signal by an imaging unit and displayed on a display device 9. The video microscope comprises: a video switching means 85 for switching a video signal to be inputted to the display device 9 between an imaging unit 4 and a D/A converting means 82; a still picture generating means 74 for giving a command to a memory control means 81 so as to repeatedly read image data for one frame from a memory 83 and controlling the video switching means 85 so as to input the video signal from the D/A converting means 82 to the display device 9; and a light source control means 11 for controlling light source feeding power so as to extinct or turn off the light source 10 while the still picture is displayed on the display device 9.
COPYRIGHT: (C)2006,JPO&NCIPI

Abstract translation: 要解决的问题：提供一种视频显微镜，其中一帧的图像数据从存储器反复读取并作为静止图像显示在显示装置上，并且在此期间控制光源馈送功率以消除光源 展示期。 解决方案：在视频显微镜中，用光源10产生的用照明光照亮的观察对象的图像经由光学放大系统3拍摄，并由成像单元转换为视频信号，并显示在显示装置 视频显微镜包括：视频切换装置85，用于切换要在输入到成像单元4和D / A转换装置82之间的显示装置9的视频信号; 用于向存储器控制装置81发出命令以从存储器83反复读取一帧的图像数据并控制视频切换装置85以便输入来自D / A转换器的视频信号的静止图像发生装置74 装置82到显示装置9; 以及光源控制装置11，用于在静止图像显示在显示装置9上时控制光源馈送功率以消除或关闭光源10.（C）2006年，JPO和NCIPI

公开(公告)号：JP2004138898A

公开(公告)日：2004-05-13

申请号：JP2002304674

申请日：2002-10-18

Applicant: Olympus Corp ,  オリンパス株式会社

Inventor： MATSUO YUICHIRO

IPC: G02B21/36

Abstract: PROBLEM TO BE SOLVED: To provide an optical apparatus capable of efficiently initializing a plurality of optical elements in a short time.
SOLUTION: In the optical apparatus having a plurality of optical elements and provided with stepping motors for the respective optical elements, the optical elements are driven by the stepping motors by making a combination answering the purpose and also can be moved to a specified position as initializing action. The apparatus has a current value storage means 384 for storing the rated current value of every stepping motor to drive the optical element. By referring to the contents of the storage means 384 by the initializing action, the combination of the stepping motors which can be driven simultaneously within the current capacity of a power source is decided by an arithmetic processing means 383, and the optical elements are successively driven to the initializing position by the stepping motors decided to be combined.
COPYRIGHT: (C)2004,JPO

公开(公告)号：JP2011027542A

公开(公告)日：2011-02-10

申请号：JP2009173472

申请日：2009-07-24

Applicant: Olympus Corp ,  オリンパス株式会社

Inventor： TAKAGI KOSUKE ,  AMAKAWA GENTA ,  SAITO KEI ,  MATSUO YUICHIRO

IPC: G01N21/64 ,  G06T1/00

CPC classification number: G06T7/0012 ,  G06K9/00147 ,  G06T2207/30024

Abstract: PROBLEM TO BE SOLVED: To provide a cell image analyzing apparatus which can appropriately automatically classify various cells under a high cell-density condition such as neuron astrocyte of nerve cells.
SOLUTION: To provide the cell image analyzing apparatus with a computer, for classifying cells using a plurality of channels of fluorescence cell images on a specimen which contains the plurality of kinds of cells and is stained with specific fluorochromes in accordance with the kinds of cells. The cell image analyzing apparatus has an image analysis software which makes the computer function as a region delimiting means 1a for delimiting cell nucleus regions and cytoplasm regions in each channel of fluorescence cell images; a morphologic characteristic detecting means 1b for detecting a morphologic characteristic on the cell nucleus regions or the cytoplasm regions delimited via the region delimiting means 1a; and a cell classifying means 1c for classifying the cells in accordance with the morphologic characteristic on the cell nucleus regions or the cytoplasm regions detected via the morphologic characteristic detecting means 1b.
COPYRIGHT: (C)2011,JPO&INPIT

Abstract translation: 解决问题的方案：提供一种细胞图像分析装置，能够适当地自动分类神经细胞的神经元星形细胞等高细胞密度条件下的各种细胞。 解决方案：为了向细胞图像分析装置提供计算机，用于在包含多种细胞的样本上使用多个荧光细胞图像的通道对细胞进行分类，并且根据种类被特定的荧光染料染色 的细胞。 细胞图像分析装置具有图像分析软件，其使计算机作为区域限定装置1a，用于限定荧光细胞图像的每个通道中的细胞核区域和细胞质区域; 用于检测经由区域限定装置1a限定的细胞核区域或细胞质区域的形态特征的形态特征检测装置1b; 以及细胞分类手段1c，用于根据通过形态特征检测装置1b检测的细胞核区域或细胞质区域的形态特征对细胞进行分类。 版权所有（C）2011，JPO＆INPIT

公开(公告)号：JP2010268723A

公开(公告)日：2010-12-02

申请号：JP2009123286

申请日：2009-05-21

Applicant: Olympus Corp ,  オリンパス株式会社

Inventor： KOBAYASHI TAMIYO ,  MATSUO YUICHIRO

IPC: C12Q1/02 ,  C12M1/34 ,  G01N21/78 ,  G01N33/48

Abstract: PROBLEM TO BE SOLVED: To provide a cell-screening method by which measuring target cells in a well can be efficiently detected, to provide a controlling soft wear, a cell-screening apparatus, and an image-analyzing apparatus usable in the method, and to provide a cell-screening system.
SOLUTION: In the cell-screening method, the cell-screening apparatus scans each well of a multiwell plate storing cells in prescribed order of the wells, and takes pictures of the whole region of the scanning well by dividing the whole region into a plurality of shots, and the image-analyzing apparatus analyzes the images for each taken shot through the cell-screening apparatus. The image-taking and image analysis for the well are finished and the scan to the following well is started when a target number of the cells of the detection targets are detected from the image of each shot in the scanning well.
COPYRIGHT: (C)2011,JPO&INPIT

Abstract translation: 解决问题的方案为了提供可以有效地检测孔中的测定目标细胞，提供控制软磨损的细胞筛选方法，细胞筛选装置和可用于图像分析装置的图像分析装置 方法，并提供细胞筛选系统。 解决方案：在细胞筛选方法中，细胞筛选装置以规定的数量顺序扫描存储细胞的多孔板的每个孔，并通过将整个区域分成全部区域来拍摄扫描井的整个区域 多个拍摄，并且图像分析装置通过细胞筛选装置分析每次拍摄的图像。 当从扫描井中的每个镜头的图像中检测到目标数目的检测目标时，开始对井的图像拍摄和图像分析，并开始到下一个井的扫描。 版权所有（C）2011，JPO＆INPIT

公开(公告)号：JP2010213582A

公开(公告)日：2010-09-30

申请号：JP2009061134

申请日：2009-03-13

Applicant: Olympus Corp ,  オリンパス株式会社

Inventor： TAKAGI KOSUKE ,  MATSUO YUICHIRO ,  KOBAYASHI TAMIYO ,  YONEKAWA HIROYUKI

IPC: C12M1/34 ,  C12Q1/02 ,  G01N21/01

CPC classification number: C12M41/36

Abstract: PROBLEM TO BE SOLVED: To provide a device for lighting cultured cells which have a simple optical constitution, quickly extracts steric information of three-dimensionally formed cultured cells in excellent quantitativity, and to provide a method for lighting cultured cells.
SOLUTION: In an apparatus for measuring cultured cells which is equipped with an image acquisition for acquiring images of cultured cells and image analysis means for performing a prescribed analysis of the images acquired through the image acquisition device, the device for lighting cultured cells is used for lighting three-dimensionally formed cultured cells, and has a light source 8 for lighting the cultured cells 3 three-dimensionally formed on a culture dish 1 arranged on an image acquisition position by the image acquisition device with scattered light from a slanting direction.
COPYRIGHT: (C)2010,JPO&INPIT

Abstract translation: 解决问题的方案为了提供具有简单的光学构成的培养细胞的照明装置，能够迅速地提取具有良好的定量性的三维培养细胞的空间信息，提供培养细胞的照明方法。 解决方案：在装备有用于获取培养细胞图像的图像采集的培养细胞测量装置和用于对通过图像获取装置获取的图像进行规定分析的图像分析装置中，用于照射培养细胞的装置 用于照明三维形成的培养细胞，并且具有光源8，其用于照射由图像获取装置布置在图像获取位置上的培养皿1上三维地形成的培养细胞3，其具有来自倾斜方向的散射光 。 版权所有（C）2010，JPO＆INPIT

公开(公告)号：JP2009186291A

公开(公告)日：2009-08-20

申请号：JP2008025777

申请日：2008-02-05

Applicant: Olympus Corp ,  オリンパス株式会社

Inventor： KOBAYASHI TAMIYO ,  TAKAGI KOSUKE ,  MATSUO YUICHIRO

IPC: G01N21/64 ,  G01N33/48 ,  G06T1/00

CPC classification number: G06K9/00127 ,  G01N21/6458 ,  G06T7/0012 ,  G06T2207/10056 ,  G06T2207/10064 ,  G06T2207/30024

Abstract: PROBLEM TO BE SOLVED: To provide a cell image analyzer or a method for determining existence of a membrane translocation reaction quickly with excellent reproducibility by using a fluorescence microscope.
SOLUTION: This cell image analyzer for determining existence of the membrane translocation reaction generated in a cell by imparting an optional stimulus to the cell includes a means for acquiring a fluorescence microscopic image after imparting the stimulus to the cell as image data, a means for determining a domain where the cell exists in a cell fluorescent image, a means for determining a contour line of the cell having the width of a prescribed number of pixels from the fringe of the domain where the cell exists, and a means for determining existence of the membrane translocation reaction based on a brightness value on the contour line of the cell and a brightness value on the inside of the contour line.
COPYRIGHT: (C)2009,JPO&INPIT

Abstract translation: 要解决的问题：提供一种细胞图像分析仪或通过使用荧光显微镜以优异的再现性快速确定膜易位反应的存在的方法。 解决方案：用于确定细胞中产生的膜易位反应的存在的细胞图像分析仪通过赋予细胞任选的刺激包括用于在将细胞作为图像数据赋予刺激之后获取荧光显微镜图像的方法， 用于确定细胞存在于细胞荧光图像中的细胞域的装置，用于从细胞存在的结构域的边缘确定具有规定数目的像素的宽度的细胞的轮廓线的装置，以及用于确定 基于细胞轮廓线上的亮度值和轮廓线内侧的亮度值，存在膜易位反应。 版权所有（C）2009，JPO＆INPIT

公开(公告)号：JP2009151023A

公开(公告)日：2009-07-09

申请号：JP2007327789

申请日：2007-12-19

Applicant: Olympus Corp ,  オリンパス株式会社

Inventor： TSUTSUI AKIRA ,  MATSUO YUICHIRO ,  HARADA MITSUO ,  SHIMADA YOSHIHIRO

IPC: G02B21/06 ,  F21V9/40 ,  G01N21/64 ,  H01L33/00 ,  H01L33/50 ,  H01L33/58 ,  H01L33/60

CPC classification number: G01N21/6428 ,  A61B5/0059 ,  G01N21/6458 ,  G01N2021/6419 ,  G01N2021/6484 ,  G01N2201/0624 ,  G01N2201/0627 ,  G02B21/06

Abstract: PROBLEM TO BE SOLVED: To provide an illumination apparatus for the cellular analysis apparatus in which the loss of light is kept to a minimum, the amount of light of the LED light sources is automatically maintained constant, and changes of the location, shape, and intensity of fluorescent light of a substance for its object can be quantitatively obtained. SOLUTION: The illumination apparatus for the cellular analysis apparatus includes: a plurality of LEDs 11R, 11G, 11B of different center emission wavelengths; a photodetector 12; a path sharing means for introducing light emitted from the plurality of LEDs into the common optical path; a light-introducing device located on the common optical path to introduce part of the light emitted from the plurality of LEDs passing through the common optical path into the photodetector; a feedback controller 15 controlling turning-on states of the LEDs over preset states in accordance with the amount of light emitted from the plurality of LEDs detected by the photodetector; and an illumination light supplying device 16 which is located on the common optical path and supplies light which is emitted from the plurality of LEDs to pass through the common optical path and is not introduced into the photodetector through the light-introducing device, as illumination light for a cellular analysis. COPYRIGHT: (C)2009,JPO&INPIT

Abstract translation: 要解决的问题：为了提供将光损失保持在最小值的细胞分析装置的照明装置，LED光源的光量自动保持恒定，并且位置的变化， 可以定量地获得物体的荧光的形状和强度。 解决方案：用于细胞分析装置的照明装置包括：具有不同中心发射波长的多个LED 11R，11G，11B; 光电检测器12; 用于将从所述多个LED发射的光引入所述公共光路中的路径共享装置; 位于公共光路上的光引入装置，用于将从通过公共光路的多个LED发射的光的一部分引入光电检测器; 反馈控制器15，根据由光检测器检测到的多个LED发出的光量，控制LED的预置状态的接通状态; 以及照明光供给装置16，其位于公共光路上，并且供给从多个LED射出的光，以通过公共光路，并且不通过光导入装置引入到光检测器中作为照明光 用于细胞分析。 版权所有（C）2009，JPO＆INPIT

公开(公告)号：JP2009064398A

公开(公告)日：2009-03-26

申请号：JP2007234077

申请日：2007-09-10

Applicant: Olympus Corp ,  オリンパス株式会社

Inventor： TAKAGI KOSUKE ,  MATSUO YUICHIRO ,  SHIMADA YOSHIHIRO

IPC: G06T1/00 ,  G01N33/48

Abstract: PROBLEM TO BE SOLVED: To provide a cell analysis method, apparatus and program that can increase the accuracy of evaluation of drug reactions of cells.
SOLUTION: The cell analysis method includes an imaging step of imaging each cell group cultured under a different condition, a luminance measurement step of measuring the luminance of each cell from the cell image acquired in the imaging step, a distribution characteristic creation step of creating distribution characteristics of the luminance data under each condition, and a normalization step of normalizing the distribution characteristics created under each condition.
COPYRIGHT: (C)2009,JPO&INPIT

Abstract translation: 要解决的问题：提供可以提高细胞药物反应评价的准确性的细胞分析方法，装置和程序。 解决方案：细胞分析方法包括对在不同条件下培养的每个细胞群进行成像的成像步骤，从在成像步骤中获取的细胞图像测量每个细胞的亮度的亮度测量步骤，分布特征产生步骤 在每个条件下产生亮度数据的分布特性，以及规范化在每个条件下产生的分布特性的归一化步骤。 版权所有（C）2009，JPO＆INPIT

公开(公告)号：JP2009003286A

公开(公告)日：2009-01-08

申请号：JP2007165553

申请日：2007-06-22

Applicant: Olympus Corp ,  オリンパス株式会社

Inventor： TAKAGI KOSUKE ,  MATSUO YUICHIRO

IPC: G02B21/24

CPC classification number: G02B21/365

Abstract: PROBLEM TO BE SOLVED: To provide an electric microscope system by which the burden of the screen operation of an observer is relieved as far as possible and operation conditions are efficiently and quickly set to make a microscope operable, and to provide electric microscope control software.
SOLUTION: The electric microscope system includes: an electric microscope section which has an illumination optical system, an electric stage, an image forming optical system having an objective lens and an image forming lens and an imaging means; a housing; a controller which has a picture display device and an arithmetic processing section; and software which displays the operation conditions setting picture of the electric microscope section on the picture display device and controls the operation of the electric microscope section on the basis of set conditions. In the electric microscope system, the operation conditions setting picture has a setting section 31a for exposure conditions by the imaging means, a setting section 32a for focusing conditions by the objective lens, a switching setting section 33a for the objective lens, a setting section 34a for the wavelength and brightness of illumination light, a setting section 35b for an imaging range, a display section 36 for a picked-up image and an operation instruction section 37 for the electric microscope section, in one picture.
COPYRIGHT: (C)2009,JPO&INPIT

Abstract translation: 要解决的问题：提供一种电动显微镜系统，其中观察者的屏幕操作的负担尽可能地减轻，并且操作条件被有效且快速地设置为使显微镜可操作，并且提供电显微镜 控制软件。 电动显微镜系统包括：具有照明光学系统，电动台，具有物镜的成像光学系统和成像透镜和成像装置的电子显微镜部分; 房屋; 控制器，具有图像显示装置和运算处理部; 以及在图像显示装置上显示电子显微镜部分的操作条件设置图片并基于设定条件控制电动显微镜部分的操作的软件。 在电动显微镜系统中，操作条件设定画面具有用于成像装置的曝光条件的设置部分31a，用于物镜聚焦的设置部分32a，用于物镜的切换设置部分33a，设置部分34a 对于照明光的波长和亮度，用于成像范围的设置部分35b，用于拾取图像的显示部分36和用于电显微镜部分的操作指令部分37。 版权所有（C）2009，JPO＆INPIT

公开(公告)号：JP2008043244A

公开(公告)日：2008-02-28

申请号：JP2006221109

申请日：2006-08-14

Applicant: Olympus Corp ,  オリンパス株式会社

Inventor： TAKAGI KOSUKE ,  MATSUO YUICHIRO ,  SHIMADA YOSHIHIRO

IPC: C12M1/34 ,  C12Q1/02 ,  G01N21/64

CPC classification number: G01N21/6486 ,  G01N21/6458 ,  G01N2021/6482

Abstract: PROBLEM TO BE SOLVED: To acquire the clear image of small-diameter projection part and to improve an analysis accuracy without using a complicated dyeing method.
SOLUTION: The apparatus 1 for automatic cell analysis is equipped with an imaging device 4 for imaging fluorescence emitted from cells S and acquiring cell images, an exposure change means 5 for changing exposure conditions in the acquisition of cell images by the imaging device 4 and a treatment means 5 for analyzing the cells S on the basis of each of a plurality of acquired cell images under the changed exposure conditions.
COPYRIGHT: (C)2008,JPO&INPIT

Abstract translation: 要解决的问题：为了获得小直径投影部分的清晰图像，并且在不使用复杂的染色方法的情况下提高分析精度。 解决方案：用于自动细胞分析的装置1配备有用于成像装置4，用于对从小区S发射的荧光进行成像并获取细胞图像;曝光改变装置5，用于改变成像装置获取细胞图像中的曝光条件 以及处理装置5，用于基于改变的曝光条件下的多个获取的细胞图像中的每一个来分析细胞S. 版权所有（C）2008，JPO＆INPIT