Abstract:
PURPOSE: Provided are a method for the fermentative preparation of L-amino acids and nucleotide sequences coding for the accDA gene by using Coryneform bacteria. CONSTITUTION: Cloned corynebacterium glutamicum DNA is replicable in coryneform microorganisms and contains at least nucleotide sequence coding accDNA gene of SEQ ID NO:1. Coryneform microorganisms transformed with one or more copies of the cloned corynebacterium glutamicum DNA. The shuttle vector pZ1accDA is contained in corynebacterium glutamicum (DSM 12785). The process for producing L-amino acids, comprises the step of culturing a coryneform bacterium that overexpresses the accDA gene.
Abstract translation:目的:提供使用棒状细菌发酵制备L-氨基酸和编码accDA基因的核苷酸序列的方法。 构成:克隆的谷氨酸棒状杆菌DNA在棒状细菌中是可复制的,并且至少含有编码SEQ ID NO:1的cDNA序列的核苷酸序列。 用克隆的谷氨酸棒杆菌DNA的一个或多个拷贝转化的棒状细菌。 穿梭载体pZ1accDA含有谷氨酸棒杆菌(DSM 12785)。 生产L-氨基酸的方法包括培养过表达accDA基因的棒状细菌的步骤。