公开(公告)号：US09890404B2

公开(公告)日：2018-02-13

申请号：US13992242

申请日：2011-12-08

Applicant: Hyang Choi ,  Kyoung Min Lee ,  Min Sun Kang ,  Sung Hoo Jhon ,  Hye Won Um ,  Su Jin Choi ,  Han Won Lee ,  Soo An Shin

Inventor： Hyang Choi ,  Kyoung Min Lee ,  Min Sun Kang ,  Sung Hoo Jhon ,  Hye Won Um ,  Su Jin Choi ,  Han Won Lee ,  Soo An Shin

IPC: C12P13/00 ,  C12N15/77 ,  C07K14/34 ,  C12N9/02 ,  C12N9/10 ,  C12N9/12 ,  C12N9/88 ,  C12R1/15

CPC classification number: C12P13/001 ,  C07K14/34 ,  C12N9/0008 ,  C12N9/1018 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/1217 ,  C12N9/88 ,  C12R1/15 ,  C12Y102/01038 ,  C12Y201/03003 ,  C12Y203/01035 ,  C12Y206/01013 ,  C12Y207/02008 ,  C12Y401/01017

Abstract: The present invention relates to a putrescine-producing microorganism and a method for producing putrescine using the same. To be more specific, the present invention is directed to a microorganism given the ability to produce putrescine which is generated by blocking a biosynthetic pathway from ornithine to arginine, increasing the intracellular level of glutamate, enhancing the biosynthetic pathway of ornithine from glutamate, and introducing extracellular ornithine decarboxylase; and a method for producing putrescine by using the microorganism.

公开(公告)号：US20170088858A1

公开(公告)日：2017-03-30

申请号：US15298760

申请日：2016-10-20

Applicant: The Trustees of the University of Pennsylvania

Inventor： Guangping Gao ,  James M. Wilson ,  Mauricio R. Alvira

IPC: C12N15/86 ,  A61K9/00 ,  A61K38/48 ,  C12N7/00 ,  C12N9/64

CPC classification number: C12N15/86 ,  A61K9/0019 ,  A61K35/761 ,  A61K38/177 ,  A61K38/45 ,  A61K38/4846 ,  A61K48/00 ,  C07K14/005 ,  C07K14/705 ,  C07K14/755 ,  C12N7/00 ,  C12N9/1018 ,  C12N9/644 ,  C12N15/8645 ,  C12N2750/14122 ,  C12N2750/14143 ,  C12N2750/14152 ,  C12N2830/008 ,  C12N2830/48 ,  C12N2830/85 ,  C12Y201/03003 ,  C12Y304/21021 ,  C12Y304/21022

Abstract: Sequences of a serotype 8 adeno-associated virus and vectors and host cells containing these sequences are provided. Also described are methods of using such host cells and vectors in production of rAAV particles.

公开(公告)号：US09587250B2

公开(公告)日：2017-03-07

申请号：US14598477

申请日：2015-01-16

Applicant: The Trustees of the University of Pennsylvania

Inventor： Guangping Gao ,  James M. Wilson ,  Mauricio R. Alvira

IPC: C12N15/86 ,  C07K14/005 ,  C07K14/755 ,  C12N7/00 ,  A61K35/761 ,  A61K38/17 ,  A61K38/45 ,  A61K38/48 ,  C07K14/705 ,  C12N9/10 ,  C12N9/64 ,  A61K48/00

CPC classification number: C12N15/86 ,  A61K9/0019 ,  A61K35/761 ,  A61K38/177 ,  A61K38/45 ,  A61K38/4846 ,  A61K48/00 ,  C07K14/005 ,  C07K14/705 ,  C07K14/755 ,  C12N7/00 ,  C12N9/1018 ,  C12N9/644 ,  C12N2750/14122 ,  C12N2750/14143 ,  C12N2750/14152 ,  C12N2830/008 ,  C12N2830/48 ,  C12N2830/85 ,  C12Y201/03003 ,  C12Y304/21021 ,  C12Y304/21022

Abstract: Sequences of a serotype 8 adeno-associated virus and vectors and host cells containing these sequences are provided. Also described are methods of using such host cells and vectors in production of rAAV particles.

Abstract translation: 提供血清型8腺相关病毒和含有这些序列的载体和宿主细胞的序列。 还描述了在生产rAAV颗粒中使用这种宿主细胞和载体的方法。

公开(公告)号：US20130344545A1

公开(公告)日：2013-12-26

申请号：US13992241

申请日：2011-12-08

Applicant: Hyang Choi ,  Kyoung Min Lee ,  Min Sun Kang ,  Sung Hoo Jhon ,  Hye Won Um ,  Su Jin Choi ,  Han Won Lee ,  Soo An Shin

Inventor： Hyang Choi ,  Kyoung Min Lee ,  Min Sun Kang ,  Sung Hoo Jhon ,  Hye Won Um ,  Su Jin Choi ,  Han Won Lee ,  Soo An Shin

IPC: C12P13/10

CPC classification number: C12P13/10 ,  C07K14/34 ,  C12N9/0008 ,  C12N9/1018 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/1217 ,  C12R1/15 ,  C12Y102/01038 ,  C12Y201/03003 ,  C12Y203/01035 ,  C12Y206/01013 ,  C12Y207/02008

Abstract: The present invention relates to a microorganism having an improved ornithine-producing ability, in which the biosynthetic pathway of arginine form ornithine is blocked, the intracellular glutamate level is increased, and the biosynthetic pathway of ornithine from glutamate is enhanced, and a method for producing ornithine using the microorganism.

Abstract translation: 本发明涉及具有改善的鸟氨酸生产能力的微生物，其中精氨酸形成鸟氨酸的生物合成途径被阻断，细胞内谷氨酸水平提高，鸟氨酸从谷氨酸生物合成途径增强， 鸟氨酸使用微生物。

公开(公告)号：US11975110B2

公开(公告)日：2024-05-07

申请号：US16966214

申请日：2019-02-01

Applicant: Translate Bio, Inc.

Inventor： Frank DeRosa ,  Michael Heartlein ,  Shrirang Karve ,  Zarna Patel

IPC: C08G63/685 ,  A61K9/00 ,  A61K9/14 ,  A61K9/51 ,  A61K38/17 ,  A61K38/45 ,  A61K48/00 ,  C07D319/12 ,  C08G63/78 ,  C12N15/113

CPC classification number: A61K9/5153 ,  A61K9/0019 ,  A61K9/14 ,  A61K9/5123 ,  A61K9/5146 ,  A61K38/1709 ,  A61K38/45 ,  C07D319/12 ,  C08G63/6852 ,  C08G63/78 ,  C12Y201/03003 ,  C12N15/113 ,  C12N2310/14 ,  C12N2310/141 ,  C12N2320/32

Abstract: Disclosed are cationic polymers comprising monomers such as those described in Formula (I). Such polymers can be useful for the preparation of therapeutic compositions [e.g., compositions comprising nucleic acids such as m RNA). Additionally, therapeutic compositions comprising these cationic, biodegradable polymers can have improved properties and reduced toxicity.

公开(公告)号：US20180187222A1

公开(公告)日：2018-07-05

申请号：US15739699

申请日：2016-03-29

Applicant: CJ Cheiljedang Corporation

Inventor： Hee Kyoung JUNG ,  Hye Won UM ,  Hong Xian LI ,  Su Jin PARK ,  Young Lyeol YANG ,  Kyoung Min LEE ,  Hyo Hyoung LEE

IPC: C12P13/10 ,  C12P13/00 ,  C12N9/10 ,  C12N9/88

CPC classification number: C12P13/10 ,  C12N9/1018 ,  C12N9/1025 ,  C12N9/88 ,  C12P13/001 ,  C12Y201/03003 ,  C12Y203/03001 ,  C12Y401/01017

Abstract: The present invention relates to a recombinant microorganism for producing putrescine or ornithine, and a method for producing putrescine or ornithine using the same. Specifically, the present invention relates to a microorganism of the genus Corynebacterium capable of producing putrescine or ornithine, in which an activity of the transcriptional regulator of sugar metabolism (SugR) is weakened, an activity of the citrate synthase (GltA) is enhanced, or both are applied; and a method for producing putrescine or ornithine using the same.

公开(公告)号：US20180105850A1

公开(公告)日：2018-04-19

申请号：US15556737

申请日：2016-03-10

Applicant: BIOPETROLIA AB

Inventor： Jiufu Qin ,  Anastasia Krivoruchko ,  Florian David ,  Bo Jiang ,  Jens Nielsen

IPC: C12P13/10 ,  C12N9/10 ,  C12N9/78 ,  C12N9/02 ,  C12N9/12 ,  C12N9/88 ,  C12P13/00

CPC classification number: C12P13/10 ,  C12N9/0008 ,  C12N9/1018 ,  C12N9/1096 ,  C12N9/1217 ,  C12N9/78 ,  C12N9/88 ,  C12N15/52 ,  C12P13/001 ,  C12Y102/01038 ,  C12Y201/03003 ,  C12Y206/01011 ,  C12Y207/02008 ,  C12Y305/03001 ,  C12Y401/01017 ,  C12Y403/02001

Abstract: The present invention relates to the provision of genetically modified microbial cells, such as yeast cells with an improved ability for producing L-ornithine and its derivatives. Overproduction of L-ornithine is obtained in the first place by the down-regulation or attenuation of specially selected genes, wherein said genes encode enzymes involved in the L-ornithine consumption and/or degradation pathways. Further L-ornithine production ability is improved by down-regulation, attenuation, deletion or overexpression of specially selected genes, wherein said genes encode enzymes and/or proteins involved in the L-ornithine ‘acetylated derivatives cycle’, L-glutamate synthesis pathways, subcellular trafficking, TCA cycle, pyruvate carboxylation pathway, respiratory electron-transport chain, and the carbon substrates' assimilation machinery. The invention additionally provides a method to produce L-ornithine with said modified eukaryotic cells.

公开(公告)号：US20170314041A1

公开(公告)日：2017-11-02

申请号：US15482431

申请日：2017-04-07

Applicant: RaNA Therapeutics, Inc.

Inventor： Frank DeRosa ,  Michael Heartlein ,  Daniel Crawford ,  Shrirang Karve

IPC: C12N15/85 ,  C12N9/10 ,  C12N9/02 ,  A61K38/18 ,  C07K14/505 ,  A61K48/00 ,  A61K38/45 ,  A61K38/44 ,  C07K14/705 ,  A61K38/17

CPC classification number: C12N15/85 ,  A61K31/7115 ,  A61K31/7125 ,  A61K38/177 ,  A61K38/1816 ,  A61K38/44 ,  A61K38/45 ,  A61K48/005 ,  C07H21/02 ,  C07K14/505 ,  C07K14/705 ,  C12N9/0071 ,  C12N9/1018 ,  C12N15/67 ,  C12N2830/50 ,  C12P21/00 ,  C12Y114/16001 ,  C12Y201/03003

Abstract: The present invention provides, among other things, multimeric coding nucleic acids that exhibit superior stability for in vivo and in vitro use. In some embodiments, a multimeric coding nucleic acid (MCNA) comprises two or more encoding polynucleotides linked via 3′ ends such that the multimeric coding nucleic acid compound comprises two or more 5′ ends.

公开(公告)号：US20160201088A1

公开(公告)日：2016-07-14

申请号：US15084615

申请日：2016-03-30

Applicant: The Trustees of the University of Pennsylvania

Inventor： Guangping Gao ,  James M. Wilsom ,  Mauricio R. Alvira

IPC: C12N15/86 ,  C12N7/00 ,  A61K38/48 ,  C12N9/64

CPC classification number: C12N15/86 ,  A61K9/0019 ,  A61K35/761 ,  A61K38/177 ,  A61K38/45 ,  A61K38/4846 ,  A61K48/00 ,  C07K14/005 ,  C07K14/705 ,  C07K14/755 ,  C12N7/00 ,  C12N9/1018 ,  C12N9/644 ,  C12N2750/14122 ,  C12N2750/14143 ,  C12N2750/14152 ,  C12N2830/008 ,  C12N2830/48 ,  C12N2830/85 ,  C12Y201/03003 ,  C12Y304/21021 ,  C12Y304/21022

Abstract: Sequences of a serotype 8 adeno-associated virus and vectors and host cells containing these sequences are provided. Also described are methods of using such host cells and vectors in production of rAAV particles.

公开(公告)号：US20160101160A1

公开(公告)日：2016-04-14

申请号：US14714871

申请日：2015-05-18

Applicant: Shire Human Genetic Therapies, Inc.

Inventor： Braydon Charles Guild ,  Michael Heartlein ,  Frank DeRosa

IPC: A61K38/47 ,  A61K48/00 ,  A61K38/27 ,  A61K38/18 ,  A61K38/45

CPC classification number: A61K38/47 ,  A61K38/1816 ,  A61K38/27 ,  A61K38/45 ,  A61K48/005 ,  C07K14/505 ,  C07K14/61 ,  C12N9/1018 ,  C12N9/2465 ,  C12N15/67 ,  C12Y201/03003 ,  C12Y302/01022

Abstract: Compositions for modulating the expression of a protein in a target cell comprising at least one RNA molecule which comprises at least one modification 5 conferring stability to the RNA, as well as related methods, are disclosed.

Abstract translation: 用于调节包含至少一个RNA分子的靶细胞中蛋白质表达的组合物，其包含至少一个赋予RNA稳定性的修饰物5以及相关方法。