Abstract:
A biological fluid sample analysis chamber and a method for analyzing a biological fluid sample is provided. The chamber includes a first chamber panel, a second chamber panel, and a plurality of beads disposed between the first chamber panel and the second chamber panel, which beads are configured to not reflect light incident to the beads in an amount that appreciably interferes with a photometric analysis of the biologic fluid.
Abstract:
A clam-shell luminometer that, when closed, completely encloses an assay reaction mixture-containing reaction vessel and some portion of a reaction carousel or ring. The luminometer includes first and second portions that are coupled to each other, a photomultiplier tube, and plural fiber optic bundles that are optically coupled to the photomultiplier tube. First ends of the fiber optic bundles are disposed adjacent to the reaction vessel in the second portion so that the fiber optic bundles completely surround the perimeter or periphery of the reaction vessel.
Abstract:
An annular optical device (100) includes an annular meso-optic (1) including an annulus (11) centered about an axis of revolution (A) and a secondary optical structure (2) substantially coaxial within the annulus (11). The secondary optical structure (2) and the annular meso-optic (1) are separated by a media (12) including a media refractive index that is lower than the refractive index of the secondary optical structure. The secondary optical structure (2) holds a specimen to be radiated by impinging electromagnetic radiation. Scattered radiation from the secondary optical structure (2) and within the annulus (11) of the annular meso-optic (1) is allowed into the annular meso-optic (1) if an angle of incidence of the scattered radiation exceeds a predetermined incidence threshold. The annular meso-optic (1) re-directs the scattered radiation to comprise re-directed radiation that is substantially parallel to the axis of revolution (A).
Abstract:
A fluorescence imaging system having an enclosure having an optical excitation and detection system and features designed to suppress or reduce background fluorescence. In certain aspects, all or a portion of the interior walls has a material finish and texture that provides a surface that absorbs at least a portion of any impinging excitation light and which has low auto-fluorescing properties. In certain aspects, a baffle structure is provided on the interior of the structure and is configured to mask portions of the interior and reduce the opening through which light impinges on the detector. In certain aspects, a platform having an optically transparent window is located in the interior of the housing structure for holding a sample for excitation by excitation light from an excitation source, wherein a light-trap structure is positioned or located on an opposite side of the platform relative to the excitation source and configured to receive and contain a substantial portion of any scattered or transmitted excitation light.
Abstract:
A solid phase extraction (SPE) cartridge for use in a fluorimetric assay comprises an elongate container (10) of a material permeable to fluorescent radiation, having an open inlet end and an open outlet end. For assay of a compound of interest, a solid adsorbent stationary phase (11) is provided in the container to adsorb the target compound from a mobile phase. To reduce background fluorescence that may interfere with the assay, at least one region (12,13) that is is at leastpartially impermeable to fluorescent radiation, for example by absorbence, scattering or refracting, is provided in or on the container wall, suitably on the outlet side and/or the inlet side of the anticipated location of a band or layer (14) of a compound of interest adsorbed in the stationary phase during an SPE procedure.
Abstract:
The invention relates to a microelectronic sensor device and a method for making optical examinations at a carrier (11), e.g. for the detection of magnetic particles (1) at a contact surface (12) of the carrier (11) by frustrated total internal reflection (FTIR). A light source (21), particularly a laser light source, with a laser modulator (22) are used for emitting an input light beam (L1) into the carrier (11) which is modulated such that optical interferences with reflections (L1') of the input light beam (L1) from the entrance window (14) or other components of the carrier (11) are reduced/minimized. This can for example be achieved by a pulsed on/off modulation in which the first relaxation minimum of a currently emitted pulse (PN) coincides in the light source (21) with the first relaxation maximum of a reflected pulse (PN-1'). By reducing the effect of interferences, the setup is less prone to disturbances from dimensional variations that are e.g. induced by thermal extension.
Abstract:
This publication discloses an apparatus and method for measuring a gas content. The apparatus includes a light transmitter (1), by means of which coherent light can be sent to the measurement object (3), a receiver (2), by means of which light that has passed through the measurement object (3) can be detected, and optical means (4, 5, 6), by means of which the light intensity of the light transmitter (1) can be aimed at the receiver (2). According to the invention, the optical means include a lens element (4), the optical axis of which is arranged essentially obliquely relative to the longitudinal axis of the measurement object (3), so that the angles of the normals of the optical boundaries relative to the measuring signal are set obliquely. The lens element (4) is both a refracting and a reflecting element, and separates the measurement object (3) from the means (1, 2, 5)
Abstract:
A flow cell (10) for transporting fluid in a radiant energy field includes a cell body (12) having a tube (20) extending therethrough including a radiant energy blocking portion integral therewith. In a particular embodiment, the cell body (12) includes one or more end caps (14, 16) having a protrusion (40) may be inserted into the tube (20) to create a fluid seal, the end caps (14, 16) including open channels for transporting fluid (28, 32) and radiant energy (26, 30) therethrough, and the tube (20) in the cell body (12) includes an efficient radiant energy transmission lining that is spaced from the end cap protrusions (40), thereby forming a gap volume in the flow cell open channel (18), which gap volume may be calibrated such that radiant energy losses may be standardized in respective flow cells transporting fluids having various indices of refraction.