Abstract:
본 발명은 모낭 유도 능력이 있는 모유두 조직의 제조 방법에 관한 것으로, 구체적으로는, 모낭 유도 능력이 있다고 알려진, 모낭으로부터 분리해낸 모유두 (dermal papilla)와 하부 결직초 (lower dermal sheath) 세포를 일반적으로 알려진 배양 배지를 이용하여 증식시킨 후, 고농도의 아미노산 및 비타민과 성장인자를 함유하는 배지에서 배양함으로써 모유두 조직을 제조하는 방법에 관한 것이다. 본 발명의 모유두 조직의 제조 방법을 이용하면 모낭 유도 능력을 가진 모유두 조직을 생체 외에서 충분한 양으로 제조할 수 있으므로, 본 발명의 방법은 생체 외 모낭의 특성 연구 및 세포 이식을 통한 대머리 치료에 유용하게 사용될 수 있다.
Abstract:
본 발명은 인공장기 제조용 생분해성 콜라겐 필름 및 그의 제조방법에 관한 것으로, 구체적으로 1.0% 미만의 콜라겐으로 콜라겐 스폰지를 제조하는 단계; 상기 콜라겐 스폰지를 글루코사미노글리칸 (glucosaminoglycan, GAG) 용액에 담가 콜라겐 필름을 형성하는 단계; 및 상기 콜라겐 필름을 가교결합시키는 단계에 의해 제조되는 생분해성 콜라겐 필름 및 그의 제조방법에 관한 것이다. 본 발명의 생분해성 콜라겐 필름은 저농도의 콜라겐 용액을 사용하여 물성을 향상시켰으며 천연 고분자만을 이용하여 이식수술시 우려되는 면역반응 (immune response)은 줄이고 생착률은 높여 다양한 인공장기의 제조를 위한 기질로 유용하게 사용될 수 있다.
Abstract:
PURPOSE: Provided are a biodegradable collagen film for manufacturing artificial organs, which is used as a substrate for manufacturing many kinds of artificial organs and has excellent tissue compatibility, and its preparing method. CONSTITUTION: The biodegradable collagen film used for artificial organs is prepared in film type by crosslinking collagens with glucosaminoglycan. The method comprises 3 steps. In the 1st step, less than 1 % collagen solution is lyophilized to prepare a collagen substrate. In the following step, the collagen substrate is immersed in glucosaminoglycan solution to dry at 4-20 deg.deg.C for 24-48 hours and to make a collagen film. In the last step, the collagen film is crosslinked.
Abstract:
PURPOSE: Are provided a biodegradable polymer scaffold for producing artificial organs, which is similar to living tissue and convenient to transport and storage and has excellent tissue compatibility, and a method for producing the same. CONSTITUTION: The biodegradable polymer scaffold is produced by cultivating a cell on biodegradable polymer scaffold, and then removing a cell with only extracellular matrix(ECM) secreted from the cell being remained. The scaffold consists of any one material selected from the group consisting of collagen, chitin, chitosan, alginate, hyaluronic acid, and derivatives thereof, PLGA, PGA, and PLA. The scaffold has a form of yarn, mesh, porous sponge, or complex structure thereof. The cell is selected from the group consisting of fibroblast, keratin cell, keratocyte, chondrocyte, bone cell, muscle cell, liver cell, stem cell, adults cell, and immortalized cell.
Abstract:
PURPOSE: A cooling device having small number of components, free from the disorder, easy to repair and install and having excellent economical efficiency by using air is provided. CONSTITUTION: An air supply piece(10) injecting air to a heat exchanger body(20) injects atmospheric air into a heat exchanger body by the guiding of a hose or a pipe by the forcible air supplier such as a blower(11) or an air compressor. At the inlet hole of the heat exchanger body flows the air supplied from the air supply piece via an inlet pipe(21). At the medial of the heat exchanger body punches a reacting chamber(22) formed in a circular hole having a smooth surface in a diameter to make possible the crossing of the swirling air and the reflected air. The end of the reacting chamber is closed, so the inlet air bumps at the end part when the inlet air swirls strongly by a swirling member(30). The reflected air reflected on the medial is sent to the inlet hole again by passing through the medial of the swirling air. And an exhaust pipe(23) is formed to be communicated with the reacting chamber. Then, the reflecting amount of the reflected air is controlled to be proportional to the swirling amount of the swirling air by regulating the gap of an outlet by using a screw of a regulation valve(50). Thereby the whole cooling efficiency is controlled.
Abstract:
A handle(32) is assembled in an assembling hole(11) on the upper part of a frame(10) molded by a synthetic resin. Many ribs of the broom are linked on the lower part of the frame(10). The frame(10) and ribs are wholly molded by a melted synthetic resin solution. This broom has a long life and a low manufacturing cost.