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    POLYMER MICROFILTRATION DEVICES, METHODS OF MANUFACTURING THE SAME AND THE USES OF THE MICROFILTRATION DEVICES
    32.
    发明申请
    POLYMER MICROFILTRATION DEVICES, METHODS OF MANUFACTURING THE SAME AND THE USES OF THE MICROFILTRATION DEVICES 审中-公开
    聚合物微滤装置，其制造方法和微滤装置的用途

    公开(公告)号：WO2013078409A2

    公开(公告)日：2013-05-30

    申请号：PCT/US2012/066390

    申请日：2012-11-21

    Applicant: CREATV MICROTECH, INC.

    Inventor： TANG, Cha-Mei ,  ZHANG, Yunqi

    IPC: B01D25/02

    CPC classification number: G01N33/5005 ,  B01D29/00 ,  B01D39/16 ,  B01D39/1692 ,  B01L3/50 ,  B01L3/502 ,  B01L3/5635 ,  B01L9/00 ,  B01L2300/0681 ,  G01N1/4005 ,  G01N1/4077 ,  G01N2001/4088 ,  Y10T29/49826 ,  Y10T29/49963

    Abstract: A microfilter comprising a polymer layer formed from epoxy-based photo-definable dry film, and a plurality of apertures each extending through the polymer layer. A microfilter comprising two or more polymer layers formed from epoxy-based photo-definable dry film, and a plurality of apertures or open areas each extending through the polymer layer. A method of forming a microfilter is also disclosed. The method includes providing a first layer of epoxy-based photo-definable dry film disposed on a substrate, exposing the first layer to energy through a mask to form a pattern, defined by the mask, in the first layer of dry film, forming, from the exposed first layer of dry film, a polymer layer having a plurality of apertures extending therethrough, the plurality of apertures having a distribution defined by the pattern, and removing the polymer layer from the substrate. Unique filter holder designs and methods appropriate to hold microfilters to collect the rare cells and allow performing assays in the filter holder are provided. The invention also describes the use of the microfilter and filter holder to collect rare cells from body fluids and perform assays. Rare cells collected on the microfilter in accordance with embodiments of the present invention can be used for medical and biological research applications.

    Abstract translation: 一种包含由环氧基可光定影干膜形成的聚合物层的微过滤器，以及遍及聚合物层的多个孔。 一种包含由环氧基可光限制的干膜形成的两个或更多个聚合物层的微过滤器，以及各自延伸穿过聚合物层的多个孔或开口区域。 还公开了一种形成微过滤器的方法。 该方法包括提供设置在基底上的第一层环氧基光可定义干膜，将第一层暴露于能量通过掩模，以在第一层干膜中形成由掩模限定的图案， 从曝光的第一层干膜，具有延伸穿过其中的多个孔的聚合物层，所述多个孔具有由图案限定的分布，以及从基底去除聚合物层。 提供了独特的过滤器支架设计和适用于容纳微过滤器以收集稀有细胞并允许在过滤器保持器中进行测定的方法。 本发明还描述了微过滤器和过滤器保持器从体液中收集稀有细胞并进行测定的用途。 根据本发明的实施方案收集在微过滤器上的稀有细胞可用于医学和生物研究应用。

    ZAP-70 DETECTION IN CHRONIC LYMPHOCYTIC LEUKEMIA
    33.
    发明申请
    ZAP-70 DETECTION IN CHRONIC LYMPHOCYTIC LEUKEMIA 审中-公开
    ZAP-70检测慢性LYMPHOCYTIC LEUKEMIA

    公开(公告)号：WO2013067397A2

    公开(公告)日：2013-05-10

    申请号：PCT/US2012/063383

    申请日：2012-11-02

    Applicant: CREATV MICROTECH, INC.

    Inventor： TANG, Cha-Mei ,  ZHU, Peixuan

    CPC classification number: G01N33/57407 ,  G01N33/57426 ,  G01N33/582 ,  G01N2800/56

    Abstract: Detection of ZAP-70 expression provides important information about disease progression and overall survival in patients with chronic lymphocytic leukemia (CLL). The invention provides methods for diagnosing CLL in a subject, as well as methods for clearly distinguishing CLL patients with aggressive form of the disease. A consistent number of B cells from patient blood is isolated and lysed to release all of the intracellular ZAP-70 protein. The released ZAP-70 protein is subsequently extracted by immunomagnetic separation followed by detection with fluorescence immunosandwich assay. The ZAP-70 fluorescence signal is measured with Signalyte TM -II spectrofluorometer. The VeriZAP TM assay is a simple, reliable, and reproducible method for quantitative detection of ZAP-70 in patient leukemic cells, and can be used as a prognostic test to distinguish indolent versus aggressive CLL patients.

    Abstract translation: ZAP-70表达检测提供了有关慢性淋巴细胞性白血病（CLL）患者疾病进展和总体生存的重要信息。 本发明提供了用于诊断受试者中CLL的方法，以及用于清楚地区分CLL患者与侵袭性疾病形式的方法。 分离来自患者血液的一致数量的B细胞并裂解以释放所有细胞内ZAP-70蛋白。 随后通过免疫磁性分离提取释放的ZAP-70蛋白，随后用荧光免疫分析法检测。 用SignalyteTM-II分光光度计测量ZAP-70荧光信号。 VeriZAPTM测定法是用于定量检测患者白血病细胞中ZAP-70的简单，可靠和可重复的方法，可用作预后评估以区分无痛与侵袭性CLL患者。

    SENSITIVE EMISSION LIGHT GATHERING AND FLOW THROUGH DETECTION SYSTEM
    34.
    发明申请
    SENSITIVE EMISSION LIGHT GATHERING AND FLOW THROUGH DETECTION SYSTEM 审中-公开
    通过检测系统的敏感发射光通量和流量

    公开(公告)号：WO2008143725A3

    公开(公告)日：2009-02-12

    申请号：PCT/US2008001910

    申请日：2008-02-13

    Applicant: CREATV MICROTECH INC ,  TANG CHA-MEI ,  AMSTUTZ III PLATTE

    Inventor： TANG CHA-MEI ,  AMSTUTZ III PLATTE

    IPC: G02B6/00

    CPC classification number: G01N21/0303 ,  G01N21/255 ,  G01N21/645

    Abstract: A luminometer is provided comprising a flow through waveguide and one or more detectors. The flow through waveguide has at least two openings and the sample is free to enter from one opening and exit from the other. The flow through waveguide can be made of material that guides emission light to a bottom end of the flow through waveguide. One or more detectors may be provided which detect the emission light coming out of the bottom of the flow through waveguide.

    Abstract translation: 提供了一种发光计，其包括流过波导管和一个或多个检测器。 通过波导的流动具有至少两个开口，并且样品自由地从一个开口进入并从另一个开口排出。 通过波导的流动可以由将发射光引导到通过波导的流动的底端的材料制成。 可以提供一个或多个检测器，其检测从通过波导的流的底部出射的发射光。

    INTEGRATED MULTISTEP BIOSENSOR
    40.
    发明申请
    INTEGRATED MULTISTEP BIOSENSOR 审中-公开
    集成多媒体生物传感器

    公开(公告)号：WO2006025866A3

    公开(公告)日：2006-11-16

    申请号：PCT/US2005007122

    申请日：2005-03-04

    Applicant: CREATV MICROTECH INC ,  TANG CHA-MEI

    Inventor： TANG CHA-MEI

    IPC: C12M1/34 ,  C12M3/00

    CPC classification number: G01N33/54373 ,  B82Y15/00 ,  B82Y30/00

    Abstract: The invention provides an integrated biosensor. The integrated bioprocessor consists of an integrated capture chamber having an analyte recognition coating and a structure supporting analyte detection, analyte growth and target nucleic acid detection. The integrated capture chamber can consist of a waveguide, a capillary tube, a mixing flow chamber or an integrated combination thereof. The integrated capture chamber also can contain an antibody or other recognition species as an analyte recognition coating, an illumination source, a radiation detector, a microfluidics handling system, a second chamber for target nucleic acid detection or a combination thereof.

    Abstract translation: 本发明提供一种集成的生物传感器。 集成的生物处理器由具有分析物识别涂层和支持分析物检测，分析物生长和靶核酸检测的结构的集成捕获室组成。 集成捕获室可以由波导，毛细管，混合流动室或其一体化组合组成。 集成捕获室还可以包含抗体或其他识别物质作为分析物识别涂层，照射源，放射线检测器，微流体处理系统，用于靶核酸检测的第二室或其组合。

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