公开(公告)号：US20130264229A1

公开(公告)日：2013-10-10

申请号：US13863922

申请日：2013-04-16

Applicant: Jeong-Min Lee ,  Seong-Jae Lee

Inventor： Jeong-Min Lee ,  Seong-Jae Lee

IPC: B65D51/28 ,  B65D25/08

CPC classification number: B65D51/2835 ,  B65D5/748 ,  B65D25/08 ,  B65D25/085 ,  B65D47/063 ,  B65D47/123 ,  B65D51/2807 ,  B65D75/5861

Abstract: A cap with a storage space for containing a secondary material includes a storage portion having a storage space and provided with a seal plate formed on a lower end of the storage space, an outer cap foldably formed on the storage portion and coupled to a spouting portion, a spouting member inserted in the spouting portion, and an operational member for breaking the seal plate when the spouting member is operation.

Abstract translation: 具有用于容纳二次材料的存储空间的盖包括具有存储空间并具有形成在存储空间的下端上的密封板的存储部分，可折叠地形成在存储部分上并连接到喷射部分的外盖 插入到喷射部分中的喷射构件，以及当喷射构件操作时用于破坏密封板的操作构件。

公开(公告)号：US08413802B2

公开(公告)日：2013-04-09

申请号：US12520415

申请日：2006-12-13

Applicant: Jeong-Min Lee ,  Seong-Jae Lee

Inventor： Jeong-Min Lee ,  Seong-Jae Lee

IPC: B65D25/08

CPC classification number: B65D51/2864 ,  B65D25/082 ,  B65D51/2835 ,  B65D81/3222

Abstract: The present invention relates to a cap assembly mounted on a discharging portion of a container for containing an ingredient different from that in accommodated in a container, which comprise a cap body having a housing formed with a chamber for storage of a secondary ingredient, a closing element provided at a lower end opening of the housing for opening the lower end opening under pressure so as to open the lower end opening.

Abstract translation: 本发明涉及一种安装在容器的排出部分上的盖组件，该容器包含与容纳在容器中的成分不同的成分，该容器包括盖主体，该主体具有形成有用于存储次要成分的室， 设置在壳体的下端开口处，用于在压力下打开下端开口以打开下端开口。

公开(公告)号：US20120279939A1

公开(公告)日：2012-11-08

申请号：US13517353

申请日：2008-12-18

Applicant: Jeong-Min Lee ,  Seong-Jae Lee

Inventor： Jeong-Min Lee ,  Seong-Jae Lee

IPC: B65D51/00

CPC classification number: B65D81/3211 ,  B65D51/28 ,  B65D51/2807 ,  B65D2251/0025 ,  B65D2251/0093 ,  B65D2251/08

Abstract: A bottle cap which is combined with a beverage container in the direction of discharge or drinking, comprised of; a main body combined with the container; a storage space inside the main body which contains additives, and; filter holes formed above and below the storage space to keep the additives within the storage space; and the additives in the storage space is soluble in the liquid in the container and discharged through a spout above, and the flow path of the liquid is characterized by being configured to lead the liquid to the storage space of the additives to facilitate dissolution of the additives.

Abstract translation: 一个与排放或饮用方向的饮料容器组合的瓶盖，包括： 与容器结合的主体; 主体内容纳有添加剂的储存空间; 过滤孔形成在储存空间的上方和下方，以将添加剂保持在储存空间内; 并且储存空间中的添加剂溶解在容器中的液体中并通过上面的喷口排出，并且液体的流动路径的特征在于将液体引导到添加剂的储存空间以便于溶解 添加剂。

公开(公告)号：US08215505B2

公开(公告)日：2012-07-10

申请号：US10592028

申请日：2005-03-08

Applicant: Jeong-Min Lee ,  Seong-Jae Lee

Inventor： Jeong-Min Lee ,  Seong-Jae Lee

IPC: B65D47/00

CPC classification number: B65D51/2878 ,  B65D51/2864 ,  Y10S215/08

Abstract: A cap assembly that can be associated with a container storing a primary material includes a lid fixed on a top of the container and having an exhausting portion projected upward, a cap main body detachably coupled to the exhausting portion of the lid and having a storage tube extending downward to define a storage chamber for storing a secondary material, and an inner cap body detachably coupled to the storage tube.

Abstract translation: 可以与容纳主要材料的容器相关联的盖组件包括固定在容器的顶部上并具有向上突出的排出部分的盖，可拆卸地联接到盖的排出部分的盖主体，并具有储存管 向下延伸以限定用于存储次级材料的储存室，以及可拆卸地联接到储存管的内盖主体。

公开(公告)号：US20110098197A1

公开(公告)日：2011-04-28

申请号：US12988722

申请日：2008-05-27

Applicant: Bong Hyun Chung ,  Yong Won Jung ,  Jeong Min Lee

Inventor： Bong Hyun Chung ,  Yong Won Jung ,  Jeong Min Lee

IPC: C40B30/04 ,  C07K19/00 ,  C12N9/96 ,  C07K1/00 ,  C40B40/10 ,  C40B50/00

CPC classification number: C07K14/315 ,  G01N33/531 ,  G01N33/533

Abstract: The present invention relates to a protein G variant comprising a mutated Fc binding domain, which is prepared by substituting cysteine for specific residues of the Fc-binding domain of protein G, and a method for preparing the same. Further, the present invention relates to a protein G variant comprising a cysteine mutated Fc binding domain that is site-selectively modified with a UV cross-linker. Further, the present invention relates to a method for UV cross-linking the protein G variant with antibody. The present invention relates to a protein G variant-antibody conjugate that is prepared by the above method. Further, the present invention provides a method for screening or analyzing antigens using the conjugate. Furthermore, the present invention provides a biochip or biosensor fabricated by linking the protein G variant to the surface of a solid support, and a method for fabricating the same. In addition, the present invention provides a method for immobilizing antibodies and analyzing antigens using the biochip or biosensor.

Abstract translation: 本发明涉及包含通过用半胱氨酸代替蛋白G的Fc结合结构域的特异性残基制备的突变Fc结合结构域的蛋白G变体及其制备方法。 此外，本发明涉及包含用UV交联剂位点选择性修饰的半胱氨酸突变的Fc结合结构域的蛋白G变体。 此外，本发明涉及一种用抗体将蛋白G变体UV交联的方法。 本发明涉及通过上述方法制备的蛋白G变体 - 抗体缀合物。 此外，本发明提供了使用该缀合物筛选或分析抗原的方法。 此外，本发明提供了通过将蛋白G变体与固体支持物的表面连接而制造的生物芯片或生物传感器及其制造方法。 此外，本发明提供了使用生物芯片或生物传感器固定抗体和分析抗原的方法。

公开(公告)号：US20100209945A1

公开(公告)日：2010-08-19

申请号：US12679982

申请日：2007-10-18

Applicant: Sang Jeon Chung ,  Bong Hyun Chung ,  Yongwon Jung ,  Hyo Jin Kang ,  Jeong Min Lee

Inventor： Sang Jeon Chung ,  Bong Hyun Chung ,  Yongwon Jung ,  Hyo Jin Kang ,  Jeong Min Lee

IPC: G01N33/53 ,  C07K7/08 ,  C07K7/06 ,  G01N1/31

CPC classification number: C07K17/00 ,  C07K7/02 ,  C07K7/08 ,  G01N33/54353 ,  G01N33/54393

Abstract: The present invention relates to a method for preparing an protein monolayer using a peptide hybrid for protein immobilization, more precisely a peptide hybrid for protein immobilization which has improved solubility by introducing a PEG linker and a proper reaction group to the oligopeptide having specific affinity to selected types of proteins and is designed to provide enough space between solid substrates and proteins immobilized, whereby various solid substrates treated by the hybrid catch specific proteins effectively on. The peptide hybrid for protein immobilization of the present invention facilitates the control of orientation of an antibody on various solid surfaces and immobilization of various antibodies of different origins or having different isotypes with different affinity. Therefore, the surface treatment technique using the peptide hybrid of the invention can be effectively used for the production of various immunosensors and immune chips.

Abstract translation: 本发明涉及一种使用肽杂交体制备蛋白质单层的方法，更准确地说是用于蛋白质固定的肽杂合物，其通过将PEG接头和适当的反应基团引入具有对所选择的特异性亲和力的寡肽而具有改善的溶解性 蛋白质类型被设计为在固体底物和固定的蛋白质之间提供足够的空间，由此通过混合物处理的各种固体底物有效捕获特异性蛋白质。 用于本发明的蛋白质固定的肽杂合物有助于控制抗体在各种固体表面上的取向，并且固定不同来源的各种抗体或具有不同亲和力的不同同种型。 因此，使用本发明的肽杂交体的表面处理技术可以有效地用于生产各种免疫传感器和免疫芯片。

公开(公告)号：US20100009450A1

公开(公告)日：2010-01-14

申请号：US12559345

申请日：2009-09-14

Applicant: Seung-Goo Lee ,  Jae-Jun Song ,  Jeong-Min Lee ,  Jae-Seok Ha

Inventor： Seung-Goo Lee ,  Jae-Jun Song ,  Jeong-Min Lee ,  Jae-Seok Ha

IPC: C12N15/63

CPC classification number: C12N15/70

Abstract: A method for cloning and expressing a target gene by homologous recombination, and more particularly a method for cloning and expressing a target gene by homologous recombination, wherein a host cell transformed with a recombinant vector and a plasmid containing a recombinase system is introduced with a linear DNA fragment comprising a target gene and a sequence having homology to the recombinant vector. Because complicated genetic engineering steps, such as the restriction enzyme treatment and ligation of a vector and a target gene, are not required, the cloning of a gene can be performed without needing a high degree of skill, and enzyme cost can be reduced. The inventive method can be effectively used for the massive, high-speed cloning and protein expression of genes, and the disclosed pRMT-iTGR system can be used as an analytical means for improving high-efficiency recombinase.

Abstract translation: 通过同源重组克隆和表达靶基因的方法，更具体地，通过同源重组克隆和表达靶基因的方法，其中用重组载体转化的宿主细胞和含有重组酶系统的质粒被引入线性 包含靶基因的DNA片段和与重组载体具有同源性的序列。 因为不需要复杂的基因工程步骤，例如限制酶处理和载体和靶基因的连接，所以可以在不需要高技能的情况下进行基因的克隆，并且可以降低酶成本。 本发明的方法可以有效地用于基因的大量高速克隆和蛋白质表达，并且所公开的pRMT-iTGR系统可以用作改进高效重组酶的分析手段。

公开(公告)号：US20090166533A1

公开(公告)日：2009-07-02

申请号：US12345250

申请日：2008-12-29

Applicant: Myoung Choul CHOI ,  Yeon Suk CHOI ,  Jeong Min LEE ,  Seung Yong KIM ,  Dong Lak KIM ,  Hyun Sik KIM ,  Jong Shin YOO ,  Stefan Karl-Heinz STAHL

Inventor： Myoung Choul CHOI ,  Yeon Suk CHOI ,  Jeong Min LEE ,  Seung Yong KIM ,  Dong Lak KIM ,  Hyun Sik KIM ,  Jong Shin YOO ,  Stefan Karl-Heinz STAHL

IPC: B01D59/44

CPC classification number: H01J49/38

Abstract: A Fourier transform ion cyclotron resonance mass spectrometer (FT-ICR MS) is provided. A preamplifier is installed as nearest to an ion cyclotron resonance (ICR) trap as possible at a detector part in the mass spectrometer, and thermal noise generated at the preamplifier is minimized by means of a cryo-cooling system to increase a signal-to-noise ratio of ion detection signals such that an ultra-low amount of specimen can be detected, which was impossible in the related art.

Abstract translation: 提供傅里叶变换离子回旋共振质谱仪（FT-ICR MS）。 在质谱仪的检测器部分，尽可能将前置放大器安装到离离子回旋共振（ICR）捕获器最近处，并且通过冷冻 - 冷却系统来减小在前置放大器处产生的热噪声，从而增加信号 - 离子检测信号的噪声比，使得可以检测到超低量的样本，这在现有技术中是不可能的。

公开(公告)号：US07354437B2

公开(公告)日：2008-04-08

申请号：US10546050

申请日：2004-02-18

Applicant: Kyong Min Shin ,  Hyo Keun Lim ,  Hyun Chul Rhim ,  Jeong Min Lee

Inventor： Kyong Min Shin ,  Hyo Keun Lim ,  Hyun Chul Rhim ,  Jeong Min Lee

IPC: A61B18/18

CPC classification number: A61N1/06 ,  A61B18/1477 ,  A61B18/1815 ,  A61B2018/00011 ,  A61B2018/00023 ,  A61B2018/00577 ,  A61B2018/1475 ,  A61B2218/002

Abstract: The object of the present invention is to provide an electrode device for high frequency thermotherapy, including a main casing, an electrode needle, a first engaging part, and a guide unit coupled to the engaging part to be longitudinally moved relative to the electrode needle. The guide unit has a guide tube to receive therein the electrode needle to expose a tip of the electrode needle to the outside of an end of the guide tube. Thus, the exposed length of the tip of the electrode needle is varied according to a position that the guide unit is coupled to the engaging part. The electrode device may further include a three-way valve -provided between the main casing and the guide unit to supply a liquid medicine or water t a desired part of a patient body.

Abstract translation: 本发明的目的是提供一种用于高频热疗的电极装置，包括主壳体，电极针，第一接合部分和与相对于电极针纵向移动的接合部分的引导单元。 引导单元具有导管，用于在其中容纳电极针以将电极针的尖端暴露于引导管的端部的外侧。 因此，电极针的尖端的暴露长度根据引导单元联接到接合部的位置而变化。 电极装置还可以包括在主壳体和引导单元之间提供的三通阀，以向患者身体的期望部分供应液体药物或水。

公开(公告)号：US20070099890A1

公开(公告)日：2007-05-03

申请号：US10562125

申请日：2004-06-25

Applicant: Sung-gyu Kim ,  Jeong-min Lee ,  Jong-ok Jeun ,  Sang-kwon Sohn ,  Kyung-il Chot ,  Joong-hyup Kim ,  Ghil-soo Nam

Inventor： Sung-gyu Kim ,  Jeong-min Lee ,  Jong-ok Jeun ,  Sang-kwon Sohn ,  Kyung-il Chot ,  Joong-hyup Kim ,  Ghil-soo Nam

IPC: A61K31/545 ,  C07D501/14

CPC classification number: C07D501/00 ,  Y02P20/55

Abstract: Disclosed herein are novel cephalosporin compounds, pharmaceutically acceptable salts thereof, and a method for preparing the compounds. The compounds show superior antibacterial activity against a wide variety of gram-positive bacteria, including methicillin-resistant Staphylococcus aureus (MRSA) strain. Accordingly, the compounds can be effectively used as antibiotics.

Abstract translation: 本文公开了新型头孢菌素化合物及其药学上可接受的盐，以及制备该化合物的方法。 该化合物对各种革兰氏阳性细菌，包括耐甲氧西林金黄色葡萄球菌（MRSA）菌株显示出优异的抗菌活性。 因此，化合物可以有效地用作抗生素。