公开(公告)号：US20170176429A1

公开(公告)日：2017-06-22

申请号：US15415276

申请日：2017-01-25

Applicant: Raindance Technologies, Inc.

Inventor： Michael L. Samuels ,  Darren Roy Link

IPC: G01N33/543 ,  C12Q1/68

CPC classification number: G01N33/54366 ,  B01F13/0062 ,  B01F13/0071 ,  C12Q1/6874 ,  C40B40/00 ,  C40B40/04 ,  C40B70/00 ,  G01N33/532

Abstract: The invention generally relates to performing sandwich assays in droplets. In certain embodiments, the invention provides methods for detecting a target analyte that involve forming a compartmentalized portion of fluid including a portion of a sample suspected of containing a target analyte and a sample identifier, a first binding agent having a target identifier, and a second binding agent specific to the target analyte under conditions that produce a complex of the first and second binding agents with the target analyte, separating the complexes, and detecting the complexes, thereby detecting the target analyte.

公开(公告)号：US20170131279A1

公开(公告)日：2017-05-11

申请号：US15415290

申请日：2017-01-25

Applicant: Raindance Technologies, Inc.

Inventor： Darren Roy Link ,  Michael L. Samuels

IPC: G01N33/573 ,  G01N33/542 ,  C12Q1/68 ,  G01N33/58

CPC classification number: G01N33/573 ,  B01F3/0807 ,  B01F5/0646 ,  B01F5/0653 ,  B01F13/0062 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00351 ,  B01J2219/00418 ,  B01J2219/00479 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00592 ,  B01J2219/00599 ,  B01J2219/0065 ,  B01J2219/00657 ,  B01J2219/00664 ,  B01J2219/00702 ,  B01J2219/0072 ,  B01J2219/00722 ,  B01J2219/0074 ,  B01J2219/00743 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/027 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0654 ,  B01L2300/0681 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/0415 ,  B01L2400/0487 ,  C12Q1/25 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/6827 ,  C40B40/04 ,  C40B50/08 ,  C40B60/10 ,  G01N21/6428 ,  G01N21/6445 ,  G01N33/5008 ,  G01N33/542 ,  G01N33/582 ,  G01N2500/00

Abstract: The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the subset. The amount of target can be determined based on the detected number.

公开(公告)号：US20170128943A1

公开(公告)日：2017-05-11

申请号：US15415156

申请日：2017-01-25

Applicant: Raindance Technologies, Inc.

Inventor： Seth Fraden ,  Hakim Boukellal ,  Yanwei Jia ,  Seila Selimovic ,  Amy Rowat ,  Jeremy Agresti ,  David A. Weitz

IPC: B01L3/00 ,  G01N1/28 ,  G01N15/14 ,  F17D1/12 ,  G01N15/02

CPC classification number: B01L3/502784 ,  B01L3/502746 ,  B01L2200/0673 ,  B01L2300/0861 ,  B01L2300/087 ,  B01L2300/0877 ,  B01L2400/0487 ,  B01L2400/0688 ,  B01L2400/0694 ,  B01L2400/082 ,  F17D1/12 ,  G01N1/28 ,  G01N15/0272 ,  G01N15/1484 ,  G01N2015/0092 ,  Y10T137/0324 ,  Y10T137/0391 ,  Y10T137/0396 ,  Y10T137/2082 ,  Y10T137/218 ,  Y10T436/2575

Abstract: Microfluidic structures and methods for manipulating fluids, fluid components, and reactions are provided. In one aspect, such structures and methods can allow production of droplets of a precise volume, which can be stored/maintained at precise regions of the device. In another aspect, microfluidic structures and methods described herein are designed for containing and positioning components in an arrangement such that the components can be manipulated and then tracked even after manipulation. For example, cells may be constrained in an arrangement in microfluidic structures described herein to facilitate tracking during their growth and/or after they multiply.

公开(公告)号：US20170002400A1

公开(公告)日：2017-01-05

申请号：US15189672

申请日：2016-06-22

Applicant: Raindance Technologies, Inc.

Inventor： John Brian Hutchison ,  Jeffrey Charles Olson ,  Darren Roy Link

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  C12Q2565/629

Abstract: The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, a method for determining the nucleic acid make-up of a sample is provided.

Abstract translation: 本发明一般涉及基于液滴的数字PCR和使用其进行目标核酸分析的方法。 在某些实施方案中，提供了用于测定样品的核酸组成的方法。

公开(公告)号：US20160289670A1

公开(公告)日：2016-10-06

申请号：US14874553

申请日：2015-10-05

Applicant: Raindance Technologies, Inc.

Inventor： Michael Samuels ,  Jeffrey Charles Olson ,  Andrew Watson ,  Keith Brown ,  Darren R. Link

IPC: C12N15/10 ,  G01N33/543

CPC classification number: C12N15/1075 ,  C12Q1/6874 ,  C40B50/08 ,  G01N33/5436 ,  G01N33/58

Abstract: The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.

公开(公告)号：US09410151B2

公开(公告)日：2016-08-09

申请号：US14226482

申请日：2014-03-26

Applicant: Raindance Technologies, Inc.

Inventor： Darren R. Link ,  Laurent Boitard ,  Jeffrey Branciforte ,  Yves Charles ,  Gilbert Feke ,  John Q. Lu ,  David Marran ,  Ahmadali Tabatabai ,  Michael Weiner ,  Wolfgang Hinz ,  Jonathan M. Rothberg

IPC: C12Q1/68 ,  C12N15/10 ,  B01F13/00 ,  B01J19/00 ,  B82Y5/00 ,  B82Y30/00 ,  C07K1/04 ,  C40B30/04 ,  C40B40/04 ,  C40B50/08 ,  C40B60/08 ,  C40B60/12 ,  C40B70/00 ,  G01N33/536 ,  G01N33/68 ,  G01N33/543 ,  C12M1/34 ,  B01L3/00 ,  B01F3/08 ,  B01L7/00

CPC classification number: C12N15/1075 ,  B01F3/0807 ,  B01F13/0071 ,  B01F13/0076 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00459 ,  B01J2219/00466 ,  B01J2219/00468 ,  B01J2219/00479 ,  B01J2219/005 ,  B01J2219/00576 ,  B01J2219/00599 ,  B01J2219/00702 ,  B01J2219/0072 ,  B01J2219/00743 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502746 ,  B01L3/502761 ,  B01L3/502784 ,  B01L3/502792 ,  B01L3/565 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/0621 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0896 ,  B01L2400/0415 ,  B01L2400/0424 ,  B01L2400/0487 ,  B01L2400/084 ,  B82Y5/00 ,  B82Y30/00 ,  C07K1/047 ,  C12Q1/6804 ,  C12Q1/6816 ,  C12Q1/6825 ,  C12Q1/6834 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q2525/161 ,  C12Q2561/119 ,  C12Q2563/103 ,  C12Q2563/107 ,  C12Q2563/159 ,  C12Q2565/537 ,  C12Q2565/629 ,  C12Q2600/16 ,  C40B30/04 ,  C40B40/04 ,  C40B50/08 ,  C40B60/08 ,  C40B60/12 ,  C40B70/00 ,  G01N21/6428 ,  G01N21/6445 ,  G01N21/6458 ,  G01N33/536 ,  G01N33/542 ,  G01N33/543 ,  G01N33/6845 ,  G01N33/6854 ,  G01N2500/10

Abstract: The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays and combinatorial chemistry. The invention provides for aqueous based emulsions containing uniquely labeled cells, enzymes, nucleic acids, etc., wherein the emulsions further comprise primers, labels, probes, and other reactants. An oil based carrier-fluid envelopes the emulsion library on a microfluidic device, such that a continuous channel provides for flow of the immiscible fluids, to accomplish pooling, coalescing, mixing, sorting, detection, etc., of the emulsion library.

Abstract translation: 本发明提供了可用于进行高通量筛选测定和组合化学的新型微流体装置和方法。 本发明提供了含有唯一标记的细胞，酶，核酸等的含水基乳液，其中乳液还包含引物，标记，探针和其它反应物。 油基载体流体在微流体装置上包封乳剂库，使得连续通道提供不混溶流体的流动，以实现乳液库的汇集，聚结，混合，分选，检测等。

公开(公告)号：US09341594B2

公开(公告)日：2016-05-17

申请号：US14511892

申请日：2014-10-10

Applicant: Raindance Technologies, Inc.

Inventor： Benjamin J. Miller ,  Brian Hutchison ,  Andrew Wilson ,  Jonathan William Larson ,  Qun Zhong ,  Yevgeny Yurkovetsky ,  Darren R. Link ,  Mark Weary

IPC: B01L3/02 ,  G01N27/447 ,  B01L3/00 ,  F17D3/01 ,  G01N21/03

CPC classification number: G01N1/38 ,  B01L3/0241 ,  B01L3/502715 ,  B01L3/502784 ,  B01L2200/061 ,  B01L2200/0673 ,  B01L2200/143 ,  B01L2300/0877 ,  F16K99/0001 ,  F16K2099/0084 ,  F17D3/01 ,  G01N27/44791 ,  G01N2021/0353 ,  Y10T137/2185 ,  Y10T137/7761 ,  Y10T137/8158 ,  Y10T137/8593 ,  Y10T436/118339

Abstract: The invention provides methods for assessing one or more predetermined characteristics or properties of a microfluidic droplet within a microfluidic channel, and regulating one or more fluid flow rates within that channel to selectively alter the predetermined microdroplet characteristic or property using a feedback control.

公开(公告)号：US09273308B2

公开(公告)日：2016-03-01

申请号：US13629227

申请日：2012-09-27

Applicant: RainDance Technologies, Inc.

Inventor： Darren R. Link ,  Michael Weiner ,  David Marran ,  Jonathan M. Rothberg

IPC: G01N1/10 ,  B01L3/00 ,  B01L3/02 ,  C12N15/10 ,  B01F3/08 ,  B01F13/00 ,  B01J19/00 ,  B03C5/00 ,  B03C5/02 ,  C12Q1/00 ,  C12Q1/68 ,  G01N15/14 ,  G01N21/05 ,  G01N21/64 ,  G01N35/08 ,  G01N27/327 ,  B01L7/00 ,  B01L9/00 ,  G01N35/00 ,  G01N21/03

CPC classification number: B01F3/0807 ,  B01F13/0062 ,  B01J19/0093 ,  B01L3/502715 ,  B01L3/502746 ,  B01L3/502784 ,  B01L3/565 ,  B01L7/525 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/165 ,  B01L2400/0415 ,  B01L2400/0424 ,  B01L2400/0487 ,  B01L2400/086 ,  B03C5/005 ,  B03C5/026 ,  C12N15/1068 ,  C12N15/1086 ,  C12Q1/00 ,  C12Q1/6806 ,  C12Q1/6837 ,  C12Q1/6844 ,  C12Q1/6855 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2565/628 ,  C12Q2565/629 ,  G01N15/147 ,  G01N21/05 ,  G01N21/64 ,  G01N27/3275 ,  G01N35/08 ,  G01N2021/0346 ,  G01N2035/00237 ,  G01N2035/00326 ,  G01N2201/024 ,  Y10T137/87571 ,  Y10T137/87619 ,  Y10T137/87652 ,  C12Q2525/191 ,  C12Q2563/159 ,  C12Q2535/101 ,  C12Q2563/149 ,  C12Q2531/113

Abstract: The present invention provides novel microfluidic substrates and methods that are useful for performing biological, chemical and diagnostic assays. The substrates can include a plurality of electrically addressable, channel bearing fluidic modules integrally arranged such that a continuous channel is provided for flow of immiscible fluids.

Abstract translation: 本发明提供了可用于进行生物学，化学和诊断测定的新型微流体底物和方法。 基板可以包括多个可电寻址的通道支承流体模块，其一体地布置成使得提供用于不混溶流体流动的连续通道。

公开(公告)号：US09228229B2

公开(公告)日：2016-01-05

申请号：US13796901

申请日：2013-03-12

Applicant: Raindance Technologies, Inc.

Inventor： Jeffrey Olson ,  Darren R. Link

IPC: C12P19/34 ,  C12Q1/68

CPC classification number: C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/686 ,  C12Q1/6883 ,  C12Q1/6886 ,  C12Q2600/172 ,  C12Q2563/159 ,  C12Q2565/629 ,  C12Q2537/143 ,  C12Q2531/113 ,  C12Q2535/131

Abstract: The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.

公开(公告)号：US20140323317A1

公开(公告)日：2014-10-30

申请号：US14248991

申请日：2014-04-09

Applicant: Raindance Technologies, Inc.

Inventor： Darren R. Link ,  Laurent Boitard ,  Jeffrey Branciforte ,  Yves Charles ,  Gilbert Feke ,  John Q. Lu ,  David Marran ,  Ahmadali Tabatabai ,  Michael Weiner ,  Wolfgang Hinz ,  Jonathan M. Rothberg

IPC: C12N15/10 ,  C12Q1/68

CPC classification number: C12N15/1075 ,  B01F3/0807 ,  B01F13/0071 ,  B01F13/0076 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00459 ,  B01J2219/00466 ,  B01J2219/00468 ,  B01J2219/00479 ,  B01J2219/005 ,  B01J2219/00576 ,  B01J2219/00599 ,  B01J2219/00702 ,  B01J2219/0072 ,  B01J2219/00743 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502746 ,  B01L3/502761 ,  B01L3/502784 ,  B01L3/502792 ,  B01L3/565 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/0621 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0896 ,  B01L2400/0415 ,  B01L2400/0424 ,  B01L2400/0487 ,  B01L2400/084 ,  B82Y5/00 ,  B82Y30/00 ,  C07K1/047 ,  C12Q1/6804 ,  C12Q1/6816 ,  C12Q1/6825 ,  C12Q1/6834 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q2525/161 ,  C12Q2561/119 ,  C12Q2563/103 ,  C12Q2563/107 ,  C12Q2563/159 ,  C12Q2565/537 ,  C12Q2565/629 ,  C12Q2600/16 ,  C40B30/04 ,  C40B40/04 ,  C40B50/08 ,  C40B60/08 ,  C40B60/12 ,  C40B70/00 ,  G01N21/6428 ,  G01N21/6445 ,  G01N21/6458 ,  G01N33/536 ,  G01N33/542 ,  G01N33/543 ,  G01N33/6845 ,  G01N33/6854 ,  G01N2500/10

Abstract: The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays and combinatorial chemistry. The invention provides for aqueous based emulsions containing uniquely labeled cells, enzymes, nucleic acids, etc., wherein the emulsions further comprise primers, labels, probes, and other reactants. An oil based carrier-fluid envelopes the emulsion library on a microfluidic device, such that a continuous channel provides for flow of the immiscible fluids, to accomplish pooling, coalescing, mixing, sorting, detection, etc., of the emulsion library.

Abstract translation: 本发明提供了可用于进行高通量筛选测定和组合化学的新型微流体装置和方法。 本发明提供了含有唯一标记的细胞，酶，核酸等的含水基乳液，其中乳液还包含引物，标记，探针和其它反应物。 油基载体流体在微流体装置上包封乳剂库，使得连续通道提供不混溶流体的流动，以实现乳液库的汇集，聚结，混合，分选，检测等。