公开(公告)号：WO2004111244A2

公开(公告)日：2004-12-23

申请号：PCT/CA2004/000896

申请日：2004-06-17

Applicant: SEMBIOSYS GENETICS INC. ,  MOLONEY, Maurice, M. ,  BOOTHE, Joseph ,  KEON, Richard ,  NYKIFORUK, Cory ,  VAN ROOIJEN, Gijs

Inventor： MOLONEY, Maurice, M. ,  BOOTHE, Joseph ,  KEON, Richard ,  NYKIFORUK, Cory ,  VAN ROOIJEN, Gijs

IPC: C12N15/82

CPC classification number: C07K14/62 ,  C12N15/8257

Abstract: Methods for the production of insulin in plants are described. In one embodiment, the present invention provides a method for the expression of insulin in plants comprising: (a) providing a chimeric nucleic acid construct comprising in the 5’ supl; to 3’ direction of transcription as operably linked components: (i) a nucleic acid sequence capable of controlling expression in plant seed cells; and (ii) a nucleic acid sequence encoding an insulin polypeptide; (b) introducing the chimeric nucleic acid construct into a plant cell; and (c) growing the plant cell into a mature plant capable of setting seed wherein the seed expresses insulin.

Abstract translation: 描述了在植物中产生胰岛素的方法。 在一个实施方案中，本发明提供了一种用于在植物中表达胰岛素的方法，包括：（a）提供嵌合的核酸构建体，其包含在5' 至3'转录方向作为可操作连接的组分：（i）能够控制植物种子细胞中表达的核酸序列; 和（ii）编码胰岛素多肽的核酸序列; （b）将嵌合核酸构建体引入植物细胞; 和（c）将植物细胞生长成能种植种子的成熟植物，其中种子表达胰岛素。

公开(公告)号：WO1998027115A1

公开(公告)日：1998-06-25

申请号：PCT/CA1997000951

申请日：1997-12-05

Applicant: SEMBIOSYS GENETICS INC. ,  MOLONEY, Maurice ,  BOOTHE, Joseph ,  VAN ROOIJEN, Gijs

Inventor： SEMBIOSYS GENETICS INC.

IPC: C07K14/415

CPC classification number: C07H21/00 ,  B01D15/08 ,  B01D15/3804 ,  B01D15/3809 ,  C01G11/003 ,  C07K1/22 ,  C07K14/415 ,  C07K16/065 ,  C08B1/00 ,  C12N9/6429 ,  C12Y304/21005

Abstract: A method for the separation of a target molecule from a mixture is described. The method employs oil bodies and their associated proteins as affinity matrices for the selective, non-covalent binding of desired target molecules. The oil body proteins may be genetically fused to a ligand having specificity for the desired target molecule. Native oil body proteins can also be used in conjunction with an oil body protein specific ligand such as an antibody or an oil body binding protein. The method allows the separation and recovery of the desired target molecules due to the difference in densities between oil bodies and aqueous solutions.

Abstract translation: 描述了从混合物中分离靶分子的方法。 该方法使用油体及其相关蛋白作为所需靶分子的选择性，非共价结合的亲和基质。 油体蛋白质可以遗传融合到对所需靶分子具有特异性的配体。 天然油体蛋白质也可以与油体蛋白质特异性配体如抗体或油体结合蛋白结合使用。 由于油体和水溶液之间的密度差异，该方法允许分离和回收所需的目标分子。

公开(公告)号：EP1212438B1

公开(公告)日：2006-05-17

申请号：EP00954241.6

申请日：2000-08-25

Applicant: SemBioSys Genetics Inc. ,  COMMONWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH ORGANISATION

Inventor： CHAUDHARY, Sarita ,  VAN ROOIJEN, Gijs ,  MOLONEY, Maurice, M. ,  SINGH, Surinder

IPC: C12N15/82 ,  A01H5/10 ,  A01H5/00 ,  C12N15/29

CPC classification number: C07K14/415 ,  C12N15/8234

Abstract: Novel methods for the expression of non-native genes in flax seeds and the seeds of other plant species are provided. The methods involve the use of seed-specific promoters obtained from flax. Additionally provided are novel flax seed-specific promoters, chimeric nucleic acid constructs comprising novel flax seed-specific promoters, transgenic plant cells, transgenic plants and transgenic plant seeds containing novel flax seed-specific promoters. The promoters and methods are useful, for example, for altering the seed oil and protein composition in flax seed or other plant seeds.

公开(公告)号：EP0636179B1

公开(公告)日：2004-09-22

申请号：EP92908088.5

申请日：1992-04-15

Applicant: SemBioSys Genetics Inc.

Inventor： MOLONEY, Maurice, M.

IPC: C12N15/29 ,  C12N15/62 ,  C12N15/82 ,  C12P21/02 ,  C12N5/10 ,  A01H5/00

CPC classification number: C07K14/415 ,  C07K14/61 ,  C07K14/8139 ,  C07K2319/02 ,  C07K2319/50 ,  C07K2319/61 ,  C12N9/6483 ,  C12N15/8234 ,  C12N15/8241 ,  C12N15/8242 ,  C12N15/8257 ,  C12Y304/23004

Abstract: Methods and compositions for use therein are described for expressing a polypeptide of interest in a seed cell as a fusion protein with an oil body protein. By this means, the fusion protein is targeted to the oil bodies of a seed cell. The oil body is easily separated from other cellular material following lysis of the seed cell, for example by using the partitioning/surface properties of the oil body. The fusion protein may be isolated for example by affinity chromatography using antibodies directed to the oil body protein. Where desired, the polypeptide of interest can be recovered by treatment of the fusion protein with for example a protease capable of recognizing a proteolytic recognition site in the oil body protein proximal to the N-terminus of the polypeptide of interest.

公开(公告)号：EP1346056A1

公开(公告)日：2003-09-24

申请号：EP01992353.1

申请日：2001-12-19

Applicant: SemBioSys Genetics Inc. ,  Syngenta Participations AG

Inventor： VAN ROOIJEN, Gijs ,  DECKERS, Harm ,  HEIFETZ, Peter, Bernard ,  BRIGGS, Steven, P. ,  DALMIA, Bipin, Kumar ,  DEL VAL, Gregg ,  ZAPLACHINSKI, Steve ,  MOLONEY, Maurice

IPC: C12N15/79

CPC classification number: C12N15/8258 ,  C12N15/8257

Abstract: Improved methods for the production of multimeric-protein-complexes, such as redox proteins and immunoglobins, in association with oil bodies are described. The redox protein is enzymatically active when prepared in association with the oil bodies. Also provided are related nucleic acids, proteins, cells, plants, and compositions.

公开(公告)号：CA2720756C

公开(公告)日：2016-07-19

申请号：CA2720756

申请日：2009-04-10

Applicant: SEMBIOSYS GENETICS INC

Inventor： GUTH JACOB ,  BOOTHE JOSEPH ,  BEAZER MITCH ,  SHAFER KENT

IPC: A61K47/10 ,  A61K8/34 ,  A61K9/127 ,  A61K47/42 ,  A61P17/00 ,  A61Q19/00

Abstract: The physical stability of oleosomes is preserved in a composition by introducing a multihydric alcohol and an acid that can reduce the pH of the composition of less than 6. The resultant composition can be useful in the manufacture of cosmetic, food, and pharmaceutical products, among others.

公开(公告)号：CY1107375T1

公开(公告)日：2012-12-19

申请号：CY081100361

申请日：2008-03-31

Applicant: SEMBIOSYS GENETICS INC

Inventor： DECKERS HARM M ,  VAN ROOIJEN GIJS ,  BOOTHE JOSEPH ,  GOLL JANIS ,  MOLONEY MAURICE ,  MAHMOUD SOHEIL SAYED

IPC: A23D7/00 ,  A23K1/16 ,  A23D7/02 ,  A23G3/00 ,  A23G3/34 ,  A23G3/40 ,  A23G3/48 ,  A23G4/00 ,  A23G4/06 ,  A23G9/32 ,  A23G9/42 ,  A23K1/165 ,  A23K1/18 ,  A23L1/00 ,  A23L9/10 ,  A23L11/00 ,  A23L23/00 ,  A23L27/18 ,  A23L27/60 ,  A23L29/10 ,  A23P1/08 ,  A61K8/00 ,  A61K8/06 ,  A61K8/18 ,  A61K8/64 ,  A61K8/73 ,  A61K8/92 ,  A61K8/96 ,  A61K8/97 ,  A61K9/00 ,  A61K9/107 ,  A61K36/00 ,  A61K36/185 ,  A61K36/23 ,  A61K36/28 ,  A61K36/286 ,  A61K36/31 ,  A61K36/42 ,  A61K36/47 ,  A61K36/48 ,  A61K36/889 ,  A61K36/899 ,  A61K38/27 ,  A61K45/00 ,  A61K47/44 ,  A61K49/00 ,  A61Q1/00 ,  A61Q1/02 ,  A61Q1/04 ,  A61Q1/06 ,  A61Q1/08 ,  A61Q1/10 ,  A61Q1/12 ,  A61Q5/00 ,  A61Q11/00 ,  A61Q13/00 ,  A61Q17/04 ,  A61Q19/00 ,  A61Q19/10 ,  C09D5/02 ,  C09K23/00 ,  C11B1/00 ,  C11B1/04 ,  C11B1/10 ,  C11B3/00 ,  C11B3/16 ,  C11B5/00 ,  C11C3/00 ,  D01F13/00

Abstract: Ηπαρούσαεφεύρεσηπαρέχεινέουςσχηματισμούςγαλακτωμάτωνοιοποίοιπεριλαμβάνουνσώματαελαίου. Ηεφεύρεσηπαρέχειεπίσηςμιαμέθοδογιατηνπαρασκευήτωνγαλακτωμάτωνκαιτηχρήσητωνγαλακτωμάτωνσεδιάφορεςοικιακέςκαιβιομηχανικέςσυνθέσεις. Ταγαλακτώματαταιριάζουνιδιαίτεραγιατηνπαρασκευήπροϊόντωντροφίμων, προϊόντωνπροσωπικήςφροντίδας, φαρμακευτικώνπροϊόντων, καιβιομηχανικώνπροϊόντων.

公开(公告)号：AU2004247762B2

公开(公告)日：2010-05-27

申请号：AU2004247762

申请日：2004-06-17

Applicant: SEMBIOSYS GENETICS INC

Inventor： BOOTHE JOSEPH ,  NYKIFORUK CORY ,  MOLONEY MAURICE M ,  KEON RICHARD ,  ROOIJEN GIJS VAN

IPC: C07K14/62 ,  A01H1/00 ,  C12N15/82

Abstract: Methods for the production of insulin in plants are described. In one embodiment, the present invention provides a method for the expression of insulin in plants comprising: (a) providing a chimeric nucleic acid construct comprising in the 5' to 3' direction of transcription as operably linked components: (i) a nucleic acid sequence capable of controlling expression in plant seed cells; and (ii) a nucleic acid sequence encoding an insulin polypeptide; (b) introducing the chimeric nucleic acid construct into a plant cell; and (c) growing the plant cell into a mature plant capable of setting seed wherein the seed expresses insulin.

公开(公告)号：AU2009234384A1

公开(公告)日：2009-10-15

申请号：AU2009234384

申请日：2009-04-10

Applicant: SEMBIOSYS GENETICS INC

Inventor： GUTH JACOB ,  FERGUSON JOHN

IPC: A61K8/34 ,  A61K9/127 ,  A61K47/10 ,  A61K47/34 ,  A61P17/00 ,  A61Q19/00

Abstract: The release rate of an active agent from oleosomes can be modulated by formulation of the oleosomes with a release control agent, such as a multihydric alcohol. In this context, oleosomes containing an active agent may be used in the preparation of a variety of formulations, including formulations for topical use.

公开(公告)号：AT405660T

公开(公告)日：2008-09-15

申请号：AT04737835

申请日：2004-06-17

Applicant: SEMBIOSYS GENETICS INC

Inventor： MOLONEY MAURICE ,  BOOTHE JOSEPH ,  KEON RICHARD ,  NYKIFORUK CORY ,  VAN ROOIJEN GIJS

IPC: C12N15/82 ,  A01H1/00 ,  A01H5/00 ,  C07K14/62

Abstract: Methods for the production of insulin in plants are described. In one embodiment, the present invention provides a method for the expression of insulin in plants comprising: (a) providing a chimeric nucleic acid construct comprising in the 5' to 3' direction of transcription as operably linked components: (i) a nucleic acid sequence capable of controlling expression in plant seed cells; and (ii) a nucleic acid sequence encoding an insulin polypeptide; (b) introducing the chimeric nucleic acid construct into a plant cell; and (c) growing the plant cell into a mature plant capable of setting seed wherein the seed expresses insulin.