Host-associated DNA sequences, primers, and probes for PCR-based identification of dog fecal pollution sources

    公开(公告)号:US09816141B2

    公开(公告)日:2017-11-14

    申请号:US14231296

    申请日:2014-03-31

    CPC classification number: C12Q1/689

    Abstract: A method for detecting dog-fecal contamination in a sample, comprising assaying the sample using a nucleotide sequence based genetic assay which comprises contacting the sample with at least one nucleic acid molecule having the nucleic acid sequence shown in SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, or 11, the nucleic acid sequence being capable of binding to a nucleic acid sequence in the sample, and detecting binding of the nucleic acid molecule to the nucleic acid sequence in the sample, wherein a presence of binding is indicative of the presence of dog-fecal contamination in the sample; the nucleic acid molecules; and a kit comprising at least two of the above-described nucleic acid molecules.

    Multi-filter chemical speciation sampler and virtual impaction particle separation inlet therefore

    公开(公告)号:US09718017B2

    公开(公告)日:2017-08-01

    申请号:US14034165

    申请日:2013-09-23

    Inventor: Paul A. Solomon

    CPC classification number: B01D45/08 G01N1/2202 G01N2001/2223

    Abstract: A multi-filter chemical speciation sampler and a virtual impaction particle separation inlet therefore are provided. The inlet includes a housing having a bottom, a collection tube that extends through the bottom, and collection apertures formed in the bottom, arranged around the collection tube; a first plate disposed on top of the housing, having acceleration nozzles disposed at the perimeter thereof; a second plate disposed in the housing below the first plate, having a central aperture and separation apertures disposed around the central aperture. The sampler includes: an inlet; a virtual impaction separator to further fractionate the PM into a course fraction and a fine fraction; a first separation assembly to divide the course fraction into coarse aliquots, comprising first filters to collect the coarse aliquots; a second separation assembly to divide the fine fraction into fine aliquots, comprising second filters to collect the fine aliquots.

    FLOW IMAGING AND MONITORING FOR SYNCHRONIZED MANAGEMENT OF WIDE AREA DRAINAGE
    35.
    发明申请
    FLOW IMAGING AND MONITORING FOR SYNCHRONIZED MANAGEMENT OF WIDE AREA DRAINAGE 有权
    流域成像和监测用于大面积排水的同步管理

    公开(公告)号:US20150090049A1

    公开(公告)日:2015-04-02

    申请号:US14281757

    申请日:2014-05-19

    Abstract: A flow imaging and monitoring system for synchronized management of wide area drainage that includes an interposer for supporting monitoring and management equipment in a manhole, a module for illuminating water flowing in pipes at the base of the manhole, a module for monitoring responses to reflected light, a sealed and rechargeable battery pack, and a data analysis and management system to interpret data streams in real time. The interposer can be adjusted to fit the diameter of the manhole and can be adjusted to be placed under the manhole cover. The module for illuminating the flowing water can be adjusted to generate various frequencies. The support structures for the modules can be adjusted for varying pitch, roll and yaw with respect to the manhole. The data analysis and management system is supported by cloud computing.

    Abstract translation: 一种用于广域排水同步管理的流量成像和监测系统,包括用于在人孔中支持监测和管理设备的插入件,用于照射在井底的管道中流动的水的模块,用于监测对反射光的响应的模块 ,密封可充电电池组,以及实时解读数据流的数据分析和管理系统。 可以调节插入器以适应人孔的直径,并可以调整放置在人孔盖下方。 用于照亮流水的模块可以调节以产生各种频率。 可以调节模块的支撑结构,以相对于人孔改变俯仰,滚动和偏航。 云计算支持数据分析和管理系统。

    RETENTION LID FOR STABILIZING POROUS MEMBRANE INSERTS DURING MANIPULATION

    公开(公告)号:US20240050953A1

    公开(公告)日:2024-02-15

    申请号:US17888153

    申请日:2022-08-15

    Abstract: A lid and a method of testing a test substance in a porous membrane insert (PMI) are dis closed, the lid including a body, sidewalls positioned along an outer perimeter of the body and configured to be seated on a shoulder of a multi-well cell culture plate, and at least one hole through the body and/or at least one knockout, wherein the lid is configured to provide the at least one hole over a corresponding well of the multi-well cell culture plate which contains the porous membrane insert. The method is a method of testing a test substance in a PMI, the method includes the steps of providing a multi-well plate with PMIs, providing the lid, removing the cover from the multi-well plate, adding the test substance, placing the lid on the multi-well cell culture plate, and conducting a manipulation of the test substance.

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