Abstract:
PURPOSE: A method for screening a material which prevents gray hair using a transgenic cell line and a vitiligo model mouse(C57bl/6-Mitfmi-vit) and a composition containing the same are provided to simultaneously recognize MITF, tyrosinase, and TRP2 changes in gene, protein, and melanin levels. CONSTITUTION: A melan-a melanocyte cell line tyrosinase-GLuc(KCLRF-BP-00163) is prepared by transforming with an expression vector, pTyrosinase-GLuc. A method for screening a material which prevents gray hair comprises: a step of treating a candidate material which prevents gray hair to the transgenic melanocyte cell line; a step of measuring the production quantity of GLuc existing in a medium; a step of applying the candidate material onto the back of a vitiligo mouse; and a step of measuring the melanin quantity of newly grown hair from the back of vitiligo mouse.
Abstract:
A white hair suppressing composition and a kit for melanogenesis promotion of hair are provided to promote melanogenesis of hair using GDNF protein or gene ciphering protein. A white hair suppressing composition comprises a neurotrophic factor receptor genesis growth factor as an active ingredient. The white hair suppressing composition promotes melanogenesis of hair by promoting expression of microphthalmia-associated transcription factor. The white hair suppressing composition is coated scalp or hair. A kit for melanogenesis promotion of hair comprises gene ciphering the neurotrophic factor receptor genesis growth factor or protein.
Abstract:
본발명은녹차다당체의제조방법에관한것으로, 보다상세하게는 a) 용매를이용하여녹차분말에서엽록소및 저분자량의폴리페놀을제거하는단계; b) 상기 a)단계의녹차잔사로부터수용성활성성분을열수추출하는단계; 및 c) 상기 b)단계의열수추출물로부터한외여과및 에탄올침전반응으로녹차다당체를분리하는단계를포함하는것을특징으로하는녹차다당체의제조방법에관한것이다. 본발명에따라제조한녹차다당체를함유하는화장료조성물은우수한피부미백, 보습및 주름개선효과를나타낸다.