Abstract:
The present invention relates to a method for extracting chlorophyll from green tea and, more specifically, to a method for extracting chlorophyll from green tea, which separates a purified form of chlorophyll from green tea in order to perform bioactive consideration of the chlorophyll separated from the green tea and to grasp characteristics regarding the use of these dye-based compounds, and tests antioxidant activity and anticancer activity of each of the separated dye-based compounds, checking whether the dye-based compounds have antioxidant and anticancer activity. According to the present invention, the method can obtain the possibility of proposing a new source of an anticancer composition while determining a new bioactive function of green tea and the effect of proposing a new function of green tea as an anticancer composition.
Abstract:
PURPOSE: A method using near infrared spectroscopy for simultaneously measuring the individual content of theanine, gava, and glutamic acid of green tea is provided to measure the individual content of theanine, gava, and glutamic acid of green tea which are effective for a tea plant breeding program simultaneously and accurately in a short time with a simple method by applying the near infrared spectroscopy. CONSTITUTION: A method using near infrared spectroscopy for simultaneously measuring the individual content of theanine, gava, and glutamic acid of green tea is as follows. Near-infrared rays are irradiated to a green tea powdered sample so that visible-near infrared absorption spectra related to the theanine, the gava, and the glutamic acid of the green tea is measured. The measured visible-near infrared absorption spectra related to the theanine, the gava, and the glutamic acid of the green tea are sorted into a visible-near infrared absorption spectrum for correcting the measurement of the content of the theanine, the gava, and the glutamic acid and a visible-near infrared absorption spectrum for verification. A far-infrared spectrum of the sorted visible-near infrared absorption spectrum for correction and a derived function thereof are obtained and a statistical analysis is performed so that each measuring formula of the theanine, the gava, and the glutamic acid is developed. The suitability of the corrected and cross-verified measuring formulas is verified after correcting and cross-verifying the measuring formulas by using the visible-near infrared absorption spectrum for correction so that an optimal measuring formula is selected.