公开(公告)号：KR1020040051383A

公开(公告)日：2004-06-18

申请号：KR1020020079309

申请日：2002-12-12

Applicant: 대한민국(농촌진흥청장)

Inventor： 홍연규 ,  조재민 ,  송석보 ,  현종내 ,  이봉춘 ,  김순철

IPC: A01N63/00

Abstract: PURPOSE: Provided is a method for manufacturing mycelia suspension having herbicidal effect on white clover(trifolium repens) by using BWC98-105(Sclerotinia sp.), thereby withering the subterranean stem and stem of the white clover continuously, and inhibiting the generation thereof. CONSTITUTION: A method for manufacturing mycelia suspension having herbicidal effect on white clover(trifolium repens) by using BWC98-105(Sclerotinia sp.) comprises the steps of: culturing BWC98-105 strain in PD broth medium, at 28 deg.C and 150rpm; forming semi-solid mycelia; growing the mycelia to aerial mycelia through static culture and forming sclerotium thereon; and forming mycelia suspension by pulverizing the mycelia of BWC98-105 strain.

Abstract translation: 目的：提供通过使用BWC98-105（核盘菌属）制备白三叶草（三叶草）具有除草效果的菌丝体悬浮液的方法，从而连续地枯萎白三叶草的地下茎和茎，并抑制其生成。 构成：通过使用BWC98-105（核盘菌属）制备对白三叶草（三叶草）具有除草效果的菌丝体悬浮液的方法包括以下步骤：在PD肉汤培养基中，在28℃和150rpm下培养BWC98-105菌株 ; 形成半固体菌丝体; 通过静态培养将菌丝体生长成空肠菌丝体，并在其上形成菌核; 并通过粉碎BWC98-105菌株的菌丝体形成菌丝体悬浮液。