公开(公告)号：KR100380535B1

公开(公告)日：2003-04-16

申请号：KR1020000021864

申请日：2000-04-25

Applicant: 조정일 ,  김형수 ,  강길진

Inventor： 조정일 ,  강길진 ,  김형수

IPC: C12N1/00 ,  C12N1/20

Abstract: PURPOSE: An antagonistic microorganism Bacillus subtilis CP220 is provided, which or 0.1-50% cultured broth of which inhibits growth of aflatoxin-producing fungi, or decomposes aflatoxin. Fermented soybean food and animal feed-stuff containing the Bacillus subtilis CP220 are also provided. CONSTITUTION: A method of isolating and identifying antagonistic microorganism, Bacillus subtilis CP220, from soil reagent or customary fermented soybeans comprises the steps of: mixing the reagent with tris-HCl buffer solution(pH 7.5); inoculating the mixture into a nutrient broth, and culturing at 20-30°C for 20-40minutes; taking some of the cultured solution, and diluting with saline(0.85% NaCl) step by step; smearing each diluted solution on a nutrient agar, culturing at 20-30°C for 3days, and isolating colony; inoculating the Aspergillus flavus and the Aspergillus parasiticus on potato dextrose agar and culturing at 20-30°C for a day; inoculating the antagonistic microorganism at four positions around the culture medium and culturing continuously; selecting the bacillus that form inhibition zone after culturing for 7days; selecting the most superior antagonistic microorganism and identifying it as the Bacillus subtilis CP220.

Abstract translation: 目的：提供一种拮抗微生物枯草芽孢杆菌CP220，其或0.1-50％的培养液抑制黄曲霉毒素产生真菌的生长或分解黄曲霉毒素。 还提供了含有枯草芽孢杆菌CP220的发酵大豆食品和动物饲料。 构成：从土壤试剂或常规发酵大豆分离和鉴定拮抗性微生物枯草芽孢杆菌CP220的方法包括以下步骤：将试剂与tris-HCl缓冲溶液（pH7.5）混合; 将该混合物接种到营养肉汤中，并在20-30℃培养20-40分钟; 取一些培养液，用盐水（0.85％NaCl）逐步稀释; 将每种稀释溶液涂抹在营养琼脂上，在20-30℃培养3天，分离菌落; 将黄曲霉和寄生曲霉接种在马铃薯葡萄糖琼脂上并在20-30℃培养一天; 在培养基周围的四个位置接种拮抗微生物并连续培养; 选择培养7天后形成抑菌圈的芽孢杆菌; 选择最优的拮抗微生物并将其鉴定为枯草芽孢杆菌CP220。

公开(公告)号：KR100319135B1

公开(公告)日：2001-12-29

申请号：KR1019990034872

申请日：1999-08-23

Applicant: 조정일

Inventor： 조정일

IPC: A01N63/02

Abstract: 본발명은식물병해에길항성을지니는미생물을분리하고, 이를제제화시킨식물병해에길항성을지니는미생물제제및 그의제조방법에관한것으로, 더욱상세하게는, 자연에서분리된식물병해에길항성을지니는바실러스속미생물바실러스서브틸리스 CP220(CP220:기탁번호 KCTC-8831P호)를배지에서성장발육시키고, 희석배율약100∼1,000배정도로희석시켜제조된식물병해길항성을지니는미생물제제에관한것이다.

公开(公告)号：KR1020000063185A

公开(公告)日：2000-11-06

申请号：KR1020000021864

申请日：2000-04-25

Applicant: 조정일 ,  김형수 ,  강길진

Inventor： 조정일 ,  강길진 ,  김형수

IPC: C12N1/00 ,  C12N1/20

Abstract: PURPOSE: An antagonistic microorganism Bacillus subtilis CP220 is provided, which or 0.1-50% cultured broth of which inhibits growth of aflatoxin-producing fungi, or decomposes aflatoxin. Fermented soybean food and animal feed-stuff containing the Bacillus subtilis CP220 are also provided. CONSTITUTION: A method of isolating and identifying antagonistic microorganism, Bacillus subtilis CP220, from soil reagent or customary fermented soybeans comprises the steps of: mixing the reagent with tris-HCl buffer solution(pH 7.5); inoculating the mixture into a nutrient broth, and culturing at 20-30°C for 20-40minutes; taking some of the cultured solution, and diluting with saline(0.85% NaCl) step by step; smearing each diluted solution on a nutrient agar, culturing at 20-30°C for 3days, and isolating colony; inoculating the Aspergillus flavus and the Aspergillus parasiticus on potato dextrose agar and culturing at 20-30°C for a day; inoculating the antagonistic microorganism at four positions around the culture medium and culturing continuously; selecting the bacillus that form inhibition zone after culturing for 7days; selecting the most superior antagonistic microorganism and identifying it as the Bacillus subtilis CP220.

Abstract translation: 目的：提供一种拮抗性微生物枯草芽孢杆菌CP220，其中0.1-50％的培养肉汤抑制产生黄曲霉毒素的真菌的生长或分解黄曲霉毒素。 还提供了含有枯草芽孢杆菌CP220的发酵大豆食品和动物饲料。 构成：从土壤试剂或常规发酵大豆中分离鉴定拮抗性微生物枯草芽孢杆菌CP220的方法包括以下步骤：将试剂与Tris-HCl缓冲溶液（pH7.5）混合; 将混合物接种到营养肉汤中，并在20-30℃下培养20-40分钟; 取一些培养液，一步一步用盐水（0.85％NaCl）稀释; 在营养琼脂上涂抹各稀释溶液，在20-30℃培养3天，分离菌落; 在马铃薯葡萄糖琼脂上接种黄曲霉和寄生曲霉，并在20-30℃下培养一天; 在培养基周围的四个位置接种拮抗性微生物并连续培养; 选择培养7天后形成抑制区的杆菌; 选择最优异的拮抗性微生物并将其鉴定为枯草芽孢杆菌CP220。

公开(公告)号：KR3001577560000S

公开(公告)日：1994-12-24

申请号：KR3019930012903

申请日：1993-07-01

Applicant: 조정일

Designer： 조정일

公开(公告)号：KR3000142490000S

公开(公告)日：1976-04-16

申请号：KR3019730001798

申请日：1973-04-11

Applicant: 조정일

Designer： 조정일

公开(公告)号：KR100416180B1

公开(公告)日：2004-01-31

申请号：KR1020000072360

申请日：2000-12-01

Applicant: 조정일 ,  나광출

Inventor： 조정일 ,  나광출

IPC: A23L2/38

Abstract: PURPOSE: Provided is a method for manufacturing an improved functional beverage made of soybean paste by adding chitosan and chitooligosaccharide, for globalization of the traditional fermented food. CONSTITUTION: A method for manufacturing an improved functional beverage made of soybean paste is characterized by the following steps: i) manufacturing a soybean paste by using Bacillus subtilis CHO99 (KCTC-18052P) which does not smell during fermentation; ii) adding 1.0-4.0wt% of water to the soybean paste; iii) liquefying the paste with one enzyme selected from alpha-amylase, beta-amylase, and cellulase and fermenting the liquefied paste with yeast; and iv) adding sweeteners and spices to 3.0-50.0wt% of water.

Abstract translation: 目的：提供一种通过添加壳聚糖和壳寡糖来制造由大豆糊制成的改良功能饮料的方法，用于传统发酵食品的全球化。 构成：制造由大豆糊制成的改进的功能性饮料的方法的特征在于以下步骤：i）通过使用在发酵期间不闻起来的枯草芽孢杆菌CHO99（KCTC-18052P）制造大豆糊; ii）向豆酱中加入1.0-4.0wt％的水; iii）用选自α-淀粉酶，β-淀粉酶和纤维素酶中的一种酶液化糊剂并用酵母发酵液化的糊剂; 和iv）将甜味剂和香料加入到3.0-50.0wt％的水中。

公开(公告)号：KR200257390Y1

公开(公告)日：2001-12-24

申请号：KR2020010027174

申请日：2001-09-05

Applicant: 조정일

Inventor： 조정일

IPC: A44B13/00

Abstract: 본 고안은 의복용 후크에 관한 것으로서 종래에는 암.수후크에 있는 봉접링의 구경이 서로 달라 암.수 후크를 고정시키기 위하여 박음질시 봉접링의 구경에 따라 세팅작업을 달리하여야 하므로 작업시간이 오래걸리는 것은 물론 암후크의 봉접링과 봉접링 사이가 서로 떨어져 있어 강도가 약한 문제점이 있는 것이다.
따라서 본 고안은 한쌍의 봉접링(12a)(12b)과 고리(11)로 암후크(10)를 형성하고, 한쌍의 봉접링(22a)(22b)과 걸림구(21)로 수후크(20)를 구성함에 있어서,철판을 프레싱하여 상기 암후크(10)와 수후크(20)를 형성하되 상기 암후크(10)의 봉접링(12a)(12b)과 고리(11)가 중앙의 보강살(13)에 의해 서로 연결되게 하고, 상기 수후크(20)의 봉접링(22a)(22b)과 걸림구(21)도 중앙 보강살(23)에 의해 서로 일체로 연결되게 형성한 것으로서 이러한 본 고안은 봉접링의 규격이 모두 같으므로 후크를 박음질로 고정시 재봉틀의 세팅작업을 한번만 하면 되므로 작업시간을 단축한 것은 물론 암후크의 고리와 양 봉접링이 모두 보강살로 이어져 있어 그만큼 강도가 향상되어 고리의 형태가 변형되는 것을 효과적으로 방지토록 한 매우 유용한 고안이다.

公开(公告)号：KR1020010096494A

公开(公告)日：2001-11-07

申请号：KR1020000065121

申请日：2000-11-03

Applicant: 조정일

Inventor： 조정일

IPC: C12N1/20

Abstract: PURPOSE: A microbial preparation added to feedstuff of livestock and used for the treatment of excreta of livestock is provided, thereby preventing diarrhea of livestock and decreasing BOD of the excreta of livestock. CONSTITUTION: The microbial preparation added to feedstuff of livestock and used for the treatment of excreta of livestock comprises 10 to 70 wt.% of Azospirillum sp. (KCTC 8989P), 1 to 60 wt.% of Streptococcus thermophilus (KCTC 8988P) and 1 to 60 wt.% of Bacillus subtilis (KCTC 8990P). The yeast and Corynebacterium glutamicum (KCTC 3339) can be further added into the microbial preparation when it is added into livestock feedstuff. The Rhodospirillum rubrum (KCTC-1372), Thiobacillus denitrificans (KCCM 31224) and Nitrosomonas europaea (KCTC 2704) can be further added into the microbial preparation when it is added into the livestock feedstuff.

Abstract translation: 目的：提供添加到牲畜饲料中并用于处理牲畜排泄物的微生物制剂，从而防止家畜腹泻，减少家畜排泄物的BOD。 构成：添加到牲畜饲料中并用于处理牲畜排泄物的微生物制剂包括10至70重量％的Azospirillum sp。 （KCTC 8989P），1至60重量％嗜热链球菌（KCTC 8988P）和1至60重量％的枯草芽孢杆菌（KCTC 8990P）。 当将酵母和谷氨酸棒杆菌（KCTC 3339）加入到家畜饲料中时，可以进一步加入微生物制剂中。 当加入到家畜饲料中时，红霉菌（KCTC-1372），脱硫硫杆菌（KCCM 31224）和亚硝化单胞菌（KCTC 2704）可进一步加入微生物制剂中。

公开(公告)号：KR1020010069777A

公开(公告)日：2001-07-25

申请号：KR1020010025684

申请日：2001-05-11

Applicant: 조정일

Inventor： 조정일

IPC: A23L1/202

CPC classification number: A23L11/20 ,  A23L5/28 ,  A23L33/10 ,  A23V2002/00 ,  A23V2300/31

Abstract: PURPOSE: A method for preparing a heathy food using fermented beans or powdered fermented beans is provided, which can be applied to various foods such as bread, biscuit, cake, or confectionery. CONSTITUTION: A method for preparing a heathy food using fermented beans comprises the steps of: (i) a first step to inoculate Bacillus subtillisCHO99 into beans, ferment for 12-36 hours to prepare odorless fermented beans; (ii) a second step to add sweetening agents, acidulant, and spice and dry for its water content to reach 8-40% in the normal temperature; (iii) a third step to powder dried fermented beans; (iv) a fourth step to mix the fermented bean powder 100wt.% with one or more seaweed powder 5-100wt.% selected from tangleweed, brown seaweed, agar, Hizikia fusiforme, or Enteromorpha, glucose 2-20wt.%, sugar 2-20wt.%, fructose 2-20wt.%, and a small amount of salt, acidulant, and spice, or an alternative step to mix the fermented bean powder 100wt.% with one or more powders 5-100wt.% selected from Jobs-tear powder, corn powder, rice powder, or buckwheat powder instead of seaweed powder; and (v) a fifth step to add other materials as ends, and then knead, and prepare various foods.

Abstract translation: 目的：提供一种使用发酵豆或粉状发酵豆制备健康食品的方法，可用于各种食品如面包，饼干，蛋糕或糖果。 构成：使用发酵豆制备健康食品的方法包括以下步骤：（i）将芽孢杆菌ATCC9999接种到豆中的第一步，发酵12-36小时以制备无味发酵豆; （ii）第二步，在常温下加入甜味剂，酸化剂和香料，使其含水量达到8-40％; （iii）粉末干燥发酵豆的第三步骤; （iv）第四步，将100重量％的发酵豆粉与一种或多种选自缠结草，褐藻，琼脂，丝状丝酵母或蛔虫的海藻粉，5〜100重量％的葡萄糖，2-20重量％的葡萄糖，糖2 -20重量％，果糖2-20重量％，少量盐，酸化剂和香料，或将100重量％的发酵豆粉与一种或多种选自工作的粉末5-100重量％混合的替代步骤 - 粉，玉米粉，米粉或荞麦粉而不是海藻粉; 和（v）添加其他材料作为末端的第五步，然后捏合并制备各种食物。

公开(公告)号：KR2019990033652U

公开(公告)日：1999-08-16

申请号：KR2019990007879

申请日：1999-05-10

Applicant: 조정일

Inventor： 조정일

IPC: E04H13/00

Abstract: 1. 발명이속한기술분야(장례문화) 2. 발명의목적 : 좁은국토를황폐화로잠식시키는것을막고분열되는정신문화를막는다. 3. 발명의구성 : 땅을깊이파고콘크리트및 석고를묽게개어이중으로외벽을기설하여조립식목관을대고석고를묽게개어많은관을성형시켜시신을안치보관하는것임 4. 발명의효과 1) 좁은면적에많은시신을처리하는효과 2) 좁은국토를황폐화로잠식시키는것을막는효과 3) 집중된성묘관례로자연을더럽히지않는통제효과