公开(公告)号：KR100536917B1

公开(公告)日：2005-12-16

申请号：KR1020020050910

申请日：2002-08-27

Applicant: 경북대학교 산학협력단

Inventor： 김종국 ,  이진형 ,  김정민 ,  이상한 ,  신기선 ,  신용규 ,  서석종 ,  황선갑

IPC: C07K14/37

Abstract: 본 발명은 유용 토착 사상균을 포함한 송이버섯 (
Tricholoma matsutake )으로부터 하이드로포빈의 분리 및 정제, 이의 유전자 염기서열 및 이를 포함하는 조성물에 관한 것으로, 본 발명은 유용 토착 사상균을 포함한 송이버섯 유래의 신규한 하이드로포빈 단백질의 분리 정제하는 방법을 제공하는 효과가 있다. 또한, 본 발명은 송이버섯에서 유래한 하이드로포빈 단백질을 코딩하는 유전자 염기서열을 제공하는 효과가 있으며 상기 염기서열은 종래에 알려진 균주들에서 유래한 하이드로포빈의 유전자 염기서열과 비교하여 매우 낮은 염기서열 상동성을 가진다.

公开(公告)号：KR1020050023562A

公开(公告)日：2005-03-10

申请号：KR1020030059893

申请日：2003-08-28

Applicant: 경북대학교 산학협력단

Inventor： 김종국 ,  이진형 ,  김정민 ,  이상한 ,  신기선 ,  김남순

IPC: C12N15/31

CPC classification number: C07K14/37

Abstract: PURPOSE: A substance hydrophobin derived from Tricholoma matsutake and a gene encoding the same are provided, which substance hydrophobin is a low molecular weight protein produced from filamentous fungi, and forms a thin protein membrane on an interfacial surface between liquid and gas phases to covert the surface properties from hydrophobic into hydrophilic or the contrary thereof, so that the substance can be useful in cosmetics and medicines. CONSTITUTION: The substance hydrophobin derived from Tricholoma matsutake has the amino acid sequence set forth in SEQ ID NO:2, wherein the amino acid sequence of hydrophobin has a structure containing 4 loops through the disulfide bonds, and exhibits Basidiomycetes-specific hydrophobic patterns. The gene encoding the substance hydrophobin derived from Tricholoma matsutake of SEQ ID NO:2 has the nucleotide sequence set forth in SEQ ID NO:1.

Abstract translation: 目的：提供一种衍生自马蹄内陷的物质疏水蛋白和编码其的基因，该物质疏水蛋白是由丝状真菌产生的低分子量蛋白质，并且在液相和气相之间的界面上形成薄的蛋白质膜以掩蔽 从疏水性到亲水性或与此相反的表面性质，使得该物质可用于化妆品和药物。 构成：源自马兜铃霉的物质疏水蛋白具有SEQ ID NO：2所示的氨基酸序列，其中疏水蛋白的氨基酸序列具有通过二硫键的4个环的结构，并且表现出担子菌特异性的疏水性模式。 编码源自SEQ ID NO：2的Tricholoma matsutake的物质疏水蛋白的基因具有SEQ ID NO：1所示的核苷酸序列。

公开(公告)号：KR1020040018844A

公开(公告)日：2004-03-04

申请号：KR1020020050910

申请日：2002-08-27

Applicant: 경북대학교 산학협력단

Inventor： 김종국 ,  이진형 ,  김정민 ,  이상한 ,  신기선 ,  신용규 ,  서석종 ,  황선갑

IPC: C07K14/37

CPC classification number: C07K14/37 ,  C12Q1/6895

Abstract: PURPOSE: An isolation and purification method of hydrophobin protein from Tricholoma matsutake including useful tradition filamentous fungi, a gene sequence thereof and a composition containing the protein and gene sequence are provided. The hydrophobin has hydrophilic and hydrophobic converting properties, so that it can be useful in cosmetic industry, brewing industry and medicine industry. CONSTITUTION: An isolation and purification method of hydrophobin protein from Tricholoma matsutake including useful tradition filamentous fungi comprises the steps of: adding 60% ethanol into a fruit body of Tricholoma matsutake, pulverizing it, and centrifuging the pulverized solution; adding distilled water into the supernatant and subjecting the mixture to dialysis for overnight; centrifuging the remaining solution at 4 deg. C and freeze-drying the supernatant; dissolving the freeze-dried substance in 25 ml of 0.05M sodium-phosphate buffer solution(pH 6.0) and 1M sodium solution and mixing them; centrifuging the mixture at room temperature; and washing the precipitated hydrophobin containing portion with 1M sodium buffer solution, 80% ethanol and a mixed solution of chloroform and methanol, and drying it. A gene encoding the hydrophobin protein from Tricholoma matsutake has the nucleotide sequence set forth in SEQ ID NO: 4.

Abstract translation: 目的：提供包括有用的传统丝状真菌，其基因序列和含有蛋白质和基因序列的组合物的来自毛癣菌的疏水蛋白的分离和纯化方法。 疏水蛋白具有亲水和疏水转化性能，因此可用于化妆品工业，酿造工业和医药工业。 构成：包括有用的传统丝状真菌的来自毛癣菌的疏水蛋白的分离和纯化方法包括以下步骤：将60％乙醇加入到毛癣菌的果实体中，粉碎并离心粉碎的溶液; 将蒸馏水加入上清液中并使混合物透析过夜; 将剩余溶液离心4℃。 并冷冻干燥上清液; 将冷冻干燥物质溶于25ml 0.05M磷酸钠缓冲溶液（pH6.0）和1M溶液中并混合; 在室温下离心混合物; 并用1M钠缓冲溶液，80％乙醇和氯仿和甲醇的混合溶液洗涤沉淀的含有疏水蛋白的部分，并干燥。 从Tricholoma matsutake编码疏水蛋白的基因具有SEQ ID NO：4所示的核苷酸序列。