Abstract:
PURPOSE: A microsatellite marker for distinguishing foreign species of Fusarium graminearum and a distinguishing method using the same are provided to quickly and accurately distinguish species and toxin of a red mold pathogen. CONSTITUTION: A primer, FgmsTG49, of sequence numbers 1 and 2 is used for specifically amplifying a microsatellite of red mold pathogen for distinguishing foreign strain, domestic strain, and mixed strain thereof among Fusarium graminearum. A method for distinguishing the foreign species among Fusarium graminearum comprises: a step of amplifying a gene using the primer; and a step of identifying the microsatellite of the amplified gene. A kit for distinguishing and diagnosing the foreign species contains the primer and a reagent for amplification.
Abstract:
PURPOSE: A microsatellite marker for distinguishing species according to the type of toxin generated from red mold pathogen and a method for distinguishing species of pathogen using the same are provided to quickly and accurately distinguish main species and toxin by analyzing PCR using FgmsGT28. CONSTITUTION: A primer, FgmsGT28, of sequence numbers 1 and 2 is able to specifically amplify a microsatellite of red mold pathogen to distinguish Fusarium graminearum which produces deoxynivalenol(DON) and Fusarium asiaticum which produces nivalenol(NIV) by PCR(Polymerization Chain Reaction). A method for distinguishing F. graminearum and F. asiaticum comprises: a step of amplifying a gene using the primer by PCR; and a step of identifying a microsatellite site of the amplified gene. A kit for diagnosing and distinguishing species of red mold pathogen contains the primer and a reagent for amplification.
Abstract:
PURPOSE: A novel plant constitutive promoter is provided to introduce expression of herbicide resistant gene and reporter gene and to develop a transgenic plant. CONSTITUTION: A plant constitutive promoter contains partial or whole base of sequence number 1. The constitutive promoter is derived from Arabidopsis thaliana. The plant constitutive promoter UK2 is produced by amplifying nucleotides of sequence numbers 6 and 7 through PCR. A recombinant expression vector pBGWFS7-PAtUK2(deposit number: KACC 95085P) contains the plant constitutive promoter UK2. A method for constitutively expressing a target protein in a plant body comprises: a step of preparing the plant expression vector with the constitutive promoter UK2; a step of transforming plant carrier with the plant expression vector; and a step of introducing transgenic plant carrier into a plant body.
Abstract:
PURPOSE: A novel plant constitutive promoter is provided to express a foreign gene regardless of expression place and to induce constitutive expression of herbicide resistant gene and reporter gene. CONSTITUTION: A plant constitutive promoter UK1 contains a partial or whole base of sequence of sequence number 1. The constitutive promoter is derived from Arabidopsis thaliana. The constitutive promoter UK1 is obtained through PCR of oligonucleotide of sequence numbers 6 and 7. A recombinant expression vector contains the constitutive promoter. The recombinant expression vector is pBGWFS7-PAtUK1(deposit number: KACC 95084P). A transgenic plant is transformed the recombinant expression vector containing the constitutive promoter.
Abstract:
PURPOSE: A root-specific expression promoter is provided to produce a useful protein and a functional material of regulating metabolism. CONSTITUTION: A root-specific promoter(PAtF10Ext) comprises a gene sequence of the sequence number 1 (SEQ ID NO:1). A root-specific promoter(PAtF10Ext) a promoter of gene with partial homology with extension-like protein having ample proline isolated from Arabidopsis thaliana. A primer for amplifying the root-specific promoter comprises an oligonucleotide of the sequence number 2 and oligonucleotide of the sequence number 3. A plant which is transformed by the promoter includes Arabidopsis thaliana and similar dicotyledoneae.
Abstract translation:目的:提供根特异性表达启动子以产生有用的蛋白质和调节代谢的功能材料。 构成:根特异性启动子(PAtF10Ext)包含序列号1(SEQ ID NO:1)的基因序列。 根特异性启动子(PAtF10Ext)与具有从拟南芥分离的大量脯氨酸的延伸样蛋白具有部分同源性的基因启动子。 用于扩增根特异性启动子的引物包含序列号2的寡核苷酸和序列号3的寡核苷酸。由启动子转化的植物包括拟南芥和类似的双子叶植物。
Abstract:
본 발명은 유전자변형 벼의 정성 및 정량분석 방법에 관한 것으로써, 보다 상세하게는 유전자변형농산물에 대한 검정방법으로 벼 염색체 DNA로부터 핵산중합효소연쇄반응(PCR) 방법에 의해 벼 내재유전자와 외래도입유전자를 특이적으로 검출함으로써 유전자변형 벼를 검사할 수 있는 방법으로 내재유전자 및 목적유전자 전체 또는 일부와 다른 발현조절부위의 DNA 영역의 전부 또는 일부를 포함한 영역을 증폭하도록 하는 프라이머 세트를 사용함으로써 다양한 계통의 재조합유전자를 판별하여 검출할 수 있음을 특징으로 하는 방법을 제공한다. 본 발명의 유전자변형 벼 검정용 PCR 프라이머, 프로브, 표준플라스미드는 GMO 검사키트로 상업화할 수 있으며, 유통 중인 유전자변형 벼의 정성 및 정량분석뿐만이 아니라 동형접합계통(homozygote line) 벼의 선별에 이용될 수 있다. 유전자변형 벼, PCR, 실시간 PCR, 특이 프라이머, 프로브, GMO 검정, 동형접합체(homozygote), 내재유전자, 도입유전자