公开(公告)号：KR101676609B1

公开(公告)日：2016-11-17

申请号：KR1020140134679

申请日：2014-10-07

Applicant: 차의과학대학교 산학협력단

Inventor： 이수홍 ,  아쉬라프사짜드 ,  박세아 ,  차병현 ,  안종찬 ,  변성은

IPC: C12N5/10 ,  C12N5/071

Abstract: 본발명은 Rheb 단백질을코딩하는유전자로연골세포를형질전환시키는단계를포함하는, 연골세포의노화또는탈분화억제방법; 및 Rheb 단백질을코딩하는유전자로형질전환된노화또는탈분화가억제된연골세포를제공한다. 본발명에따른노화또는탈분화억제방법은연골세포의단층배양에서나타나는노화및 탈분화를억제함으로써, 정상적증식능및 표현형을갖는연골세포를대량으로배양할수 있다. 또한, 본발명에따른방법은자가연골조직에서의연골세포의낮은회수율을개선할수 있어, 세포치료용연골세포제조에유용하게활용될수 있다.

公开(公告)号：KR101287861B1

公开(公告)日：2013-07-18

申请号：KR1020110015369

申请日：2011-02-22

Applicant: 차의과학대학교 산학협력단

Inventor： 이수홍 ,  강선웅 ,  김재환 ,  한인보 ,  신동아

IPC: C12N5/077 ,  C12N5/0775 ,  C12N5/02 ,  C12N15/09

Abstract: 본 발명은 (a) TGF-β 수용체를 코딩하는 유전자로 지방-유래 중간엽 줄기세포를 형질전환하는 단계; 및 (b) 단계(a)에서 얻어진 형질전환된 중간엽 줄기세포를 TGF-β를 포함하는 연골세포 분화용 배지 중에서 배양하는 단계를 포함하는, 지방-유래 중간엽 줄기세포의 연골 세포로의 분화방법을 제공한다. 또한, 본 발명은 중간엽 줄기세포의 TGF-β 수용체의 발현량을 측정하는 것을 포함하는, 중간엽 줄기세포의 연골세포로의 분화능의 분석방법을 제공한다.

公开(公告)号：KR1020120096150A

公开(公告)日：2012-08-30

申请号：KR1020110015369

申请日：2011-02-22

Applicant: 차의과학대학교 산학협력단

Inventor： 이수홍 ,  강선웅 ,  김재환 ,  한인보 ,  신동아

IPC: C12N5/077 ,  C12N5/0775 ,  C12N5/02 ,  C12N15/09

Abstract: PURPOSE: An analytical method of mesenchyme stem cell to chondrocyte and differentiation method of the lipid- originated mesenchyme stem cell to the chondrocyte are provided to effectively analyze differentiation of mesenchyme stem cell to chondrocyte by measuring expression amount of TGF- β receptor of the mesenchyme stem cells. CONSTITUTION: A differentiation method to chondrocyte of lipid-originated mesenchyme stem cell comprises the following steps: transforming the lipid-originated mesenchyme stem cell into the gene coding the TGF- β receptor; and cultivating the TGF- β the transformed mesenchyme stem cell in the culture medium for the chondrogenesis. The transformation is processed by electroporation. The medium for differentiating the chondrogenesis is a DMEM medium which includes TGF-β, dexamethasone, ascorbic acid, insulin, transferrin and selenium salt. The culture medium for cartilage differentiation includes 1-100 ng/ml of TGF-β, 10-1,000 nanoM of dexamethasone, 10~500 nanoM of ascorbic acid, 1-1000 ng/ml of insulin, 50-5000 micro gram/ml of ES and 100-3000 ng/ml of selenium salt. The analytical method of differentiation from mesenchyme stem cell to the chondrocyte is performed by measuring expression ratio of the TGF- β receptor of the mesenchyme stem cell.

Abstract translation: 目的：提供间质干细胞与软骨细胞的分析方法和脂质起始间质干细胞向软骨细胞分化的方法，通过测量间充质干细胞中TGF-β受体的表达量，有效分析间充质干细胞向软骨细胞的分化 细胞。 构成：脂质起始间质干细胞软骨细胞的分化方法包括以下步骤：将脂质起始的间质干细胞转化为编码TGF-β受体的基因; 并在培养基中培养TGF-β转化的间质干细胞用于软骨形成。 转化通过电穿孔处理。 用于分化软骨形成的介质是包含TGF-β，地塞米松，抗坏血酸，胰岛素，转铁蛋白和硒盐的DMEM培养基。 用于软骨分化的培养基包括1-100ng / ml的TGF-β，10-1,000mg的地塞米松，10〜500nm的抗坏血酸，1-1000ng / ml的胰岛素，50-5000μg/ ml的 ES和100-3000ng / ml硒盐。 通过测量间充质干细胞的TGF-β受体的表达比例，从间质干细胞向软骨细胞分化的分析方法。

公开(公告)号：KR1020120090483A

公开(公告)日：2012-08-17

申请号：KR1020110010937

申请日：2011-02-08

Applicant: 차의과학대학교 산학협력단

Inventor： 이수홍 ,  차병현 ,  김진수 ,  박상규 ,  한인보 ,  신동아

IPC: C12N5/077 ,  C12N5/074 ,  C12N5/02

Abstract: PURPOSE: A method for differentiating adult stem cells to chodrocytes or osteocytes using tauroursodeoxycholic acid is provided to selectively suppress differentiation into adipocytes. CONSTITUTION: A method for differentiating adult stem cells to chodrocytes comprises a step of culturing adult stem cells in a medium for differentiation into chondrocytes containing tauroursodeoxycholic acid. The adult stem cells are adipose-derived stem cells. The medium is a DMEM medium containing tauroursodeoxycholic acid, TGF-1 beta, dexamethasone, ascorbic acid, insulin, transferring, and selenium.

Abstract translation: 目的：提供使用牛磺熊去氧胆酸将成体干细胞分化为胆汁细胞或骨细胞的方法，以选择性抑制脂肪细胞的分化。 构成：用于将成体干细胞分化为chodrocyte的方法包括在培养基中培养成体干细胞以分化成含有葡萄糖脱氧胆酸的软骨细胞的步骤。 成体干细胞是脂肪来源的干细胞。 培养基是含有牛磺熊去氧胆酸，TGF-β1，地塞米松，抗坏血酸，胰岛素，转移和硒的DMEM培养基。

公开(公告)号：KR1020110092508A

公开(公告)日：2011-08-18

申请号：KR1020100011966

申请日：2010-02-09

Applicant: 차의과학대학교 산학협력단

Inventor： 이수홍 ,  강선웅 ,  김진수 ,  차병현

IPC: A61L27/14 ,  A61L27/38 ,  A61L27/56 ,  C12N11/02

CPC classification number: A61L27/56 ,  A61L27/58 ,  A61L27/14 ,  A61L27/38 ,  A61L27/3804 ,  A61L27/3895 ,  C12N11/02

Abstract: PURPOSE: A manufacturing method of a supporter for tissue regeneration is provided to substitute alkylcarbonyl group for the hydrogen of a terminal hydroxy group. CONSTITUTION: A manufacturing method of a supporter for tissue regeneration includes a step of performing a thermal process on a reformed biodegradable polymer that is obtained by substituting alkylcarbonyl group for the hydrogen of a terminal hydroxy group of a biodegradable polymer. The biodegradable polymer is selected from a group consisting of a copolymer of polylactide, polyglycolide, poiycaprolactone, lactide, and glycolide, a copolymer of lactide and carprolactone, and a copolymer of glycolide and carprolactone.

Abstract translation: 目的：提供用于组织再生的支持体的制造方法，以取代烷基羰基作为末端羟基的氢。 构成：用于组织再生的支持体的制造方法包括对通过用生物降解性聚合物的末端羟基的氢代替烷基羰基而获得的重整生物可降解聚合物进行热处理的步骤。 可生物降解聚合物选自聚丙交酯，聚乙交酯，聚己内酯，丙交酯和乙交酯的共聚物，丙交酯和己内酯的共聚物，以及乙交酯和己内酯的共聚物。

公开(公告)号：KR100744445B1

公开(公告)日：2007-08-01

申请号：KR1020060049546

申请日：2006-06-01

Applicant: (주) 차바이오텍 ,  차의과학대학교 산학협력단

Inventor： 정형민 ,  이수홍 ,  김시내 ,  김민정

IPC: C12N5/071 ,  C12N5/00

Abstract: A method for culturing human embryonic stem cells is provided to maintain interaction between undifferentiated human embryonic stem cells and feeder cells attached to a porous membrane, and inhibit contamination caused by enzyme treatment and feeder cells by separating only cultured stem cells from the porous membrane without enzyme treatment. The human embryonic stem cells are cultured in a medium containing the porous membrane having feeder cells in the bottom which is made of polyethylene terephthalate, polyethersulfone, polyvinylidene fluoride, cellulose, nylon, polyethylene, polypropylene, polycarbonate, polyurethane, polyacrylate, polycaprolactone and copolymers thereof and has pore diameter of 0.1-2.5 mum, wherein the feeder cell is mouse fibroblast, mouse embryonic fibroblast, human embryonic fibroblast, human bone marrow cell or adult epidermal cell.

Abstract translation: 提供培养人胚胎干细胞的方法，以保持未分化的人胚胎干细胞和附着于多孔膜的饲养细胞之间的相互作用，并且通过仅从没有酶的多孔膜分离培养的干细胞来抑制由酶处理和饲养细胞引起的污染 治疗。 将人胚胎干细胞在由聚对苯二甲酸乙二醇酯，聚醚砜，聚偏二氟乙烯，纤维素，尼龙，聚乙烯，聚丙烯，聚碳酸酯，聚氨酯，聚丙烯酸酯，聚己内酯及其共聚物制成的底部具有饲养细胞的多孔膜的培养基中培养 孔径为0.1-2.5μm，其中饲养细胞为小鼠成纤维细胞，小鼠胚胎成纤维细胞，人胚胎成纤维细胞，人骨髓细胞或成人表皮细胞。