METHODS OF DETECTING MICROORGANISMS AND KITS THEREFORE
    38.
    发明公开
    METHODS OF DETECTING MICROORGANISMS AND KITS THEREFORE 有权
    方法用于检测微生物和试剂盒THEREFOR

    公开(公告)号:EP2516666A1

    公开(公告)日:2012-10-31

    申请号:EP10798676.2

    申请日:2010-12-17

    CPC classification number: G01N21/643 C12Q1/04 C12Q1/06

    Abstract: A system for detecting microorganisms in a test sample is provided that includes a fluorescently detectable product having both acidic and basic species, wherein the fluorescently detectable product is the reaction product of (a) an enzyme substrate that comprises an enzymatically hydrolysable group and a fluorescent group and (b) a test sample comprising a microorganism having an enzyme that hydrolyzes the hydrolysable group from the fluorescent group of the enzyme substrate. The fluorescently detectable product has an excitation isosbestic point Exlambdaiso where the absorbance of the acid species is the same as the absorbance of the basic species. The system also includes a first light source having a wavelength of Exlambdaiso for irradiating the fluorescently detectable product, The fluorescently detectable product emits light at a wavelength of Emlambda1, and the quantity of light emitted at the wavelength Emlambda1 is substantially constant across a pH range of 2.5 to 8.0.

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