公开(公告)号：CA2862731A1

公开(公告)日：2013-08-08

申请号：CA2862731

申请日：2013-01-29

Applicant: BASF SE

Inventor： KUPPERS TOBIAS ,  STEFFEN VICTORIA ,  EICHSTADT RENEE CHARLOTT ,  EVERS STEFAN ,  MAURER KARL-HEINZ ,  BONGAERTS JOHANNES ,  HELLMUTH HENDRIK ,  WEBER THOMAS ,  O'CONNELL TIMOTHY

IPC: C12P21/02 ,  C12N1/21 ,  C12N9/14 ,  C12N15/00 ,  C12N15/55 ,  C12N15/75 ,  C12P1/04

Abstract: The aim of the invention is to increase the product yield of protein in a microbial fermentation process. Said aim is achieved by means of a method in which an expression construct is introduced into a microorganism of the species Bacillus pumilus, which expression construct comprises a promoter and a nucleic acid that codes for the protein, and the protein is expressed in said expression construct.

公开(公告)号：CA2835746A1

公开(公告)日：2012-12-06

申请号：CA2835746

申请日：2012-05-25

Applicant: BASF SE

Inventor： DEGERING CHRISTIAN ,  EGGERT THORSTEN ,  EVERS STEFAN ,  MAURER KARL-HEINZ ,  BONGAERTS JOHANNES

IPC: C12N15/00

Abstract: The aim of the invention is to improve the secretion of a protein from a host cell in order to increase the product yield of protein in a fermentation process. This is achieved by an expression vector comprising a) a promoter sequence and b) a nucleic acid sequence that codes for a protein. The protein comprises a signal peptide and an additional amino acid sequence, and the signal peptide comprises an amino acid sequence that is at least 80% identical to the amino acid sequence specified in SEQ ID NO. 2, at least 80% identical to the amino acid sequence specified in SEQ ID NO. 4, at least 80% identical to the amino acid sequence specified in SEQ ID NO. 6, or the signal peptide comprises an amino acid sequence that is structurally homologous to at least one of said sequences.

公开(公告)号：CA2831473A1

公开(公告)日：2012-10-18

申请号：CA2831473

申请日：2012-04-05

Applicant: BASF SE

Inventor： MAKSYM LUKAS ,  KNAB RAMONA ,  EVERS STEFAN ,  MAURER KARL-HEINZ ,  BONGAERTS JOHANNES

IPC: C12N15/67 ,  C12N1/00

Abstract: The aim of the invention is to increase the product yield of protein in microbial fermentation. Said aim is achieved by a method in which, in addition to a first expression construct coding for the protein, a second expression construct is introduced in a microorganism, which second expression construct codes for an auxiliary protease that differs from the protein, is proteolytically active and comprises an amino acid sequence, which is at least 50% identical to the amino acid sequence provided in SEQ ID NO. 1.