公开(公告)号：CA1014366A

公开(公告)日：1977-07-26

申请号：CA211485

申请日：1974-10-16

Applicant: BLOCK ENGINEERING

Inventor： HIRSCHFELD TOMAS

IPC: G01N15/02 ,  G01N21/64 ,  G01N33/49 ,  G06F17/18 ,  G06M11/00

Abstract: 1481451 Measuring the mass and distribution of particles BLOCK ENG Inc 16 Sept 1974 [23 Oct 1973] 40233/74 Heading G1A In an apparatus for measuring the mass and distribution of particles, e.g. viruses, in a sample 20 that may be treated chemically so that they fluoresce, the light emanating from the particles after they have been subjected to radiation from a laser source 22 is received by a photo-multiplier 30. The light reaching the photo-multiplier passes through a spatial filter 38, e.g. a lire screen, microscope type viewing optics 24 and a filter 26 which is restricted to the fluorescent wavelengths. The detector's output is passed via a D.C. filter 32 and a R.M.S. converter 34 to provide steady state signals representative of "T", the average total D.C. signal in the detector output, and "R", the root mean square value of the oscillations in the detector output. These signals are then processed as shown to supply two display devices representing the statistical particle distribution N=T 2 /(R 2 -T), or the mass M of the dye bound to each particle M = (R 2 -T)/KT. An alternative similiar digital signal processing system Fig. 2 (not shown), is disclosed.

公开(公告)号：CA1002343A

公开(公告)日：1976-12-28

申请号：CA200800

申请日：1974-05-24

Applicant: BLOCK ENGINEERING

Inventor： HIRSCHFELD TOMAS

IPC: G01N33/48 ,  G01N15/02 ,  G01N21/64

Abstract: Free floating viruses are detected and sized by a method which combines fluorescent staining with the observation of a modulation of the fluorescence intensity by Brownian motion of the particles in combination with a spatial filter. Intensity modulation and fluorescence data provides a set of descriptors useful in distinguishing the type of virus involved, particularly if also combined with data regarding the scattering of light by the particles. The method and apparatus are also usable in connection with detection and classification of other biological particles having the same order of magnitude of size as viruses.

公开(公告)号：AU6072373A

公开(公告)日：1975-03-27

申请号：AU6072373

申请日：1973-09-26

Applicant: BLOCK ENGINEERING

Inventor： HIRSCHFELD TOMAS

IPC: G01N33/48 ,  G01N15/14 ,  G01N21/26 ,  G01N33/16

公开(公告)号：DE2426519A1

公开(公告)日：1975-02-20

申请号：DE2426519

申请日：1974-05-31

Applicant: BLOCK ENGINEERING

Inventor： HIRSCHFELD TOMAS

IPC: G01N33/536 ,  G01N33/533 ,  G01N33/16

公开(公告)号：CA1084732A

公开(公告)日：1980-09-02

申请号：CA246754

申请日：1976-02-27

Applicant: BLOCK ENGINEERING

Inventor： HIRSCHFELD TOMAS ,  BROWN DAVID C A G

IPC: G01N30/32 ,  G01N31/08

Abstract: An improved high resolution chromatograph device operable at column pressures ranging between one and fifty atmospheres absolute measured at the column exit, means for closely regulating the column carrier gas velocity including intermittent stop flow operation, and sample collection chamber means to facilitate contamination free handling and concentration of collected pure sample during spectral or other analysis of eluted samples. Velocity and stop flow control without loss of chromatographic resolution permits matching operation time of chromatograph to that of spectral or other analysis devices.

公开(公告)号：CA1041422A

公开(公告)日：1978-10-31

申请号：CA200645

申请日：1974-05-23

Applicant: BLOCK ENGINEERING

Inventor： HIRSCHFELD TOMAS

IPC: G01N33/536 ,  G01N33/533 ,  G01N31/22

Abstract: Dye loading in specific antibody fluorescence is enhanced by grouping dye molecules on a carrier backbone polymer having reactive sites along its length and a reactive end site and bonding the end site to the antibody to be dye tagged. The reactive end site of the carrier molecule is chemically protected while bonding dye molecules along the length of the carrier molecule and the chemical protection is stripped in preparation for antibody tagging.

公开(公告)号：CA1015975A

公开(公告)日：1977-08-23

申请号：CA215298

申请日：1974-12-05

Applicant: BLOCK ENGINEERING

Inventor： HIRSCHFELD TOMAS

IPC: G01N33/48 ,  G01N21/64 ,  G01N21/77 ,  G01N33/00

Abstract: 1484580 Spectrographic analysis BLOCK ENG Inc 4 Dec 1974 [19 Dec 1973] 45169/75 Divided out of 1484579 Heading G2J A biological fluorescent dye mixture especially for use in the process disclosed in parent Specification comprises acronol phloxine and either berberine sulphate or astrazone orange.

公开(公告)号：DE2557419A1

公开(公告)日：1976-06-24

申请号：DE2557419

申请日：1975-12-19

Applicant: BLOCK ENGINEERING

Inventor： HIRSCHFELD TOMAS ,  EATON DIANE

IPC: G01N33/536 ,  C08G73/04 ,  G01N33/533 ,  G01N33/16

公开(公告)号：CA989199A

公开(公告)日：1976-05-18

申请号：CA231856

申请日：1975-07-21

Applicant: BLOCK ENGINEERING

Inventor： HIRSCHFELD TOMAS

公开(公告)号：CA988319A

公开(公告)日：1976-05-04

申请号：CA183106

申请日：1973-10-11

Applicant: BLOCK ENGINEERING

Inventor： HIRSCHFELD TOMAS