公开(公告)号：US08460879B2

公开(公告)日：2013-06-11

申请号：US11707385

申请日：2007-02-16

Applicant: David R. Walt ,  David M. Rissin

Inventor： David R. Walt ,  David M. Rissin

IPC: G01N33/53 ,  G01N33/569 ,  C12Q1/00 ,  C12Q1/68

CPC classification number: G01N33/581 ,  C12Q1/6837 ,  G01N33/543 ,  G01N33/54346 ,  G01N33/54366 ,  G01N33/54386 ,  G01N2333/938 ,  C12Q2527/143

Abstract: Arrays of single molecules and methods of producing an array of single molecules are described. Arrays with defined volumes between 10 attoliters and 50 picoliters enable single molecule detection and quantitation.

Abstract translation: 描述单分子阵列和产生单分子阵列的方法。 在10个载体和50个皮升之间具有定义体积的数组使得能够进行单分子检测和定量。

公开(公告)号：US08460878B2

公开(公告)日：2013-06-11

申请号：US11707383

申请日：2007-02-16

Applicant: David R. Walt ,  David M. Rissin

Inventor： David R. Walt ,  David M. Rissin

IPC: G01N33/53 ,  G01N33/569 ,  C12Q1/00 ,  C12Q1/68

CPC classification number: G01N33/581 ,  C12Q1/6837 ,  G01N33/543 ,  G01N33/54346 ,  G01N33/54366 ,  G01N33/54386 ,  G01N2333/938 ,  C12Q2527/143

Abstract: Arrays of single cells and methods of producing an array of single cells are described. Arrays with defined volumes between 10 attoliters and 50 picoliters enable single cell capture, detection and quantitation.

Abstract translation: 描述单细胞阵列和产生单细胞阵列的方法。 具有10个载体和50个皮升的定义体积的数组可实现单细胞捕获，检测和定量。

公开(公告)号：US08338776B2

公开(公告)日：2012-12-25

申请号：US12664340

申请日：2008-06-25

Applicant: David R. Walt ,  Alexei R. Faustov

Inventor： David R. Walt ,  Alexei R. Faustov

IPC: H01S1/00

CPC classification number: G21K1/006 ,  G02B17/0615 ,  G02B17/0636

Abstract: Methods and devices are provided for the trapping, including optical trapping; analysis; and selective manipulation of particles on an optical array. A device parcels a light source into many points of light transmitted through a microlens optical array and an Offner relay to an objective, where particles may be trapped. Preferably the individual points of light are individually controllable through a light controlling device. Optical properties of the particles may be determined by interrogation with light focused through the optical array. The particles may be manipulated by immobilizing or releasing specific particles, separating types of particles, etc.

Abstract translation: 提供了捕获的方法和装置，包括光学捕获; 分析; 以及选择性地操纵光学阵列上的颗粒。 器件将光源包裹到通过微透镜光学阵列传输的许多光点中，并将光源继电器包裹到物镜，在该物镜上可能会捕获颗粒。 优选地，各个光点可以通过光控制装置单独控制。 颗粒的光学性质可以通过光聚焦的光学阵列询问来确定。 可以通过固定或释放特定颗粒，分离颗粒等来操纵颗粒。

公开(公告)号：US08236574B2

公开(公告)日：2012-08-07

申请号：US12731130

申请日：2010-03-24

Applicant: David C. Duffy ,  David M. Rissin ,  David R. Walt ,  David Fournier ,  Cheuk Kan

Inventor： David C. Duffy ,  David M. Rissin ,  David R. Walt ,  David Fournier ,  Cheuk Kan

IPC: G01N33/543

CPC classification number: G01N33/54393 ,  G01N33/54306 ,  G01N33/54313

Abstract: The present invention relates to systems and methods for detecting analyte molecules or particles in a fluid sample and in some cases, determining a measure of the concentration of the molecules or particles in the fluid sample. Methods of the present invention may comprise immobilizing a plurality of analyte molecules or particles with respect to a plurality of capture objects. At least a portion of the plurality of capture objects may be spatially separated into a plurality of locations. A measure of the concentration of analyte molecules in a fluid sample may be determined, at least in part, on the number of reaction vessels comprising an analyte molecule immobilized with respect to a capture object. In some cases, the assay may additionally comprise steps including binding ligands, precursor labeling agents, and/or enzymatic components.

Abstract translation: 本发明涉及用于检测流体样品中的分析物分子或颗粒的系统和方法，并且在某些情况下，确定流体样品中分子或颗粒的浓度的量度。 本发明的方法可以包括相对于多个捕获物体固定多个分析物分子或颗粒。 多个捕获对象的至少一部分可以在空间上分离成多个位置。 可以至少部分地确定包含相对于捕获对象固定的分析物分子的反应容器的数量，来测量流体样品中分析物分子的浓度。 在一些情况下，该测定可另外包括包括结合配体，前体标记试剂和/或酶组分的步骤。

公开(公告)号：US08222047B2

公开(公告)日：2012-07-17

申请号：US12236486

申请日：2008-09-23

Applicant: David C. Duffy ,  Evan Ferrell ,  Jeffrey D. Randall ,  David M. Rissin ,  David R. Walt

Inventor： David C. Duffy ,  Evan Ferrell ,  Jeffrey D. Randall ,  David M. Rissin ,  David R. Walt

IPC: G01N33/543

CPC classification number: G01N33/54313 ,  C12Q1/6825 ,  G01N33/54326 ,  G01N33/54386

Abstract: The present invention relates to systems and methods for detecting analyte molecules or particles in a fluid sample and in some cases, determining a measure of the concentration of the molecules or particles in the fluid sample. Methods of the present invention may comprise immobilizing a plurality of analyte molecules or particles to form a plurality of complexes, releasing at least a portion of some of the plurality of complexes, determining at least a portion of the plurality of complexes released, and determining a measure of the concentration of the analyte molecules or particles in a fluid sample.

Abstract translation: 本发明涉及用于检测流体样品中的分析物分子或颗粒的系统和方法，并且在某些情况下，确定流体样品中分子或颗粒的浓度的量度。 本发明的方法可以包括固定多个分析物分子或颗粒以形成多个复合物，释放所述多个复合物中的至少一部分，确定所述多个复合物的至少一部分被释放，并且确定 测量流体样品中分析物分子或颗粒的浓度。

公开(公告)号：US08206917B2

公开(公告)日：2012-06-26

申请号：US13159302

申请日：2011-06-13

Applicant: Mark S. Chee ,  David R. Walt

Inventor： Mark S. Chee ,  David R. Walt

IPC: C12Q1/68 ,  C12P19/34

CPC classification number: C12Q1/6874 ,  B01J2219/005 ,  B01J2219/00572 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00702 ,  B01J2219/00722 ,  C07B2200/11 ,  C07H21/00 ,  C12Q1/6837 ,  C40B20/04 ,  C40B40/06 ,  C40B70/00 ,  C12Q2563/161 ,  C12Q2563/185 ,  C12Q2525/179 ,  C12Q2563/155 ,  C12Q2565/514

Abstract: Methods disclosed herein relate to identification of nucleotides in a nucleotide sequence.

Abstract translation: 本文公开的方法涉及鉴定核苷酸序列中的核苷酸。

公开(公告)号：US08189191B2

公开(公告)日：2012-05-29

申请号：US12239628

申请日：2008-09-26

Applicant: Michael R. Webb ,  Christopher Lafratta ,  David R. Walt

Inventor： Michael R. Webb ,  Christopher Lafratta ,  David R. Walt

IPC: G01J3/28

CPC classification number: G01J3/18 ,  G01J3/02 ,  G01J3/0208 ,  G01J3/0229 ,  G01J3/0237 ,  G01J3/0264 ,  G01J3/14 ,  G01J3/32 ,  G02B21/06 ,  G02B21/367

Abstract: Disclosed herein are systems that include: (a) an objective lens system configured to collect light from a sample; (b) a first aperture positioned to allow a portion of the collected light received from the objective lens system to pass as input light; (c) a first lens positioned to transmit the input light received from the first aperture; (d) a dispersive element configured to spatially disperse the input light received from the first lens in a first plane; (e) a second lens positioned to transmit the spatially dispersed light; (f) a second aperture positioned to allow a portion of the spatially dispersed light received from the second lens to pass as detection light; and (g) a detector positioned to receive the detection light and configured to form at least one image of the sample.

Abstract translation: 这里公开的系统包括：（a）被配置为收集来自样品的光的物镜系统; （b）第一孔，定位成允许从物镜系统接收的一部分收集的光作为输入光通过; （c）第一透镜，定位成透射从第一孔接收的输入光; （d）分散元件，被配置为在第一平面中将从第一透镜接收的输入光空间分散; （e）定位成透射空间分散的光的第二透镜; （f）第二孔，定位成允许从第二透镜接收的空间分散的光的一部分作为检测光通过; 和（g）定位成接收检测光并被配置成形成样品的至少一个图像的检测器。

公开(公告)号：US20110251105A1

公开(公告)日：2011-10-13

申请号：US13108713

申请日：2011-05-16

Applicant: David R. Walt ,  Karri Lynn Michael-Ballard

Inventor： David R. Walt ,  Karri Lynn Michael-Ballard

IPC: C40B40/00

CPC classification number: G01N15/1456 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00459 ,  B01J2219/00466 ,  B01J2219/005 ,  B01J2219/00524 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00704 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/0074 ,  B82Y30/00 ,  C12Q1/6837 ,  C40B40/06 ,  C40B40/10 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/6456 ,  G01N21/7703 ,  G01N21/78 ,  G01N33/543 ,  G01N33/54313 ,  G01N2015/1438 ,  G01N2021/6441 ,  G01N2021/6484 ,  G01N2035/0097 ,  G01N2201/0826 ,  Y02A50/55 ,  Y10S435/808 ,  Y10S436/805

Abstract: A microsphere-based analytic chemistry system and method for making the same is disclosed in which microspheres or particles carrying bioactive agents may be combined randomly or in ordered fashion and dispersed on a substrate to form an array while maintaining the ability to identify the location of bioactive agents and particles within the array using an optically interrogatable, optical signature encoding scheme. A wide variety of modified substrates may be employed which provide either discrete or non-discrete sites for accommodating the microspheres in either random or patterned distributions. The substrates may be constructed from a variety of materials to form either two-dimensional or three-dimensional configurations. In a preferred embodiment, a modified fiber optic bundle or array is employed as a substrate to produce a high density array. The disclosed system and method have utility for detecting target analytes and screening large libraries of bioactive agents.

Abstract translation: 公开了一种基于微球的分析化学体系及其制备方法，其中携带生物活性剂的微球或颗粒可以随机或以有序的方式组合并分散在基质上以形成阵列，同时保持识别生物活性物质的位置的能力 阵列内的代理和粒子，使用光学可询问的光学签名编码方案。 可以使用各种各样的改性底物，其提供用于以随机或图案分布容纳微球体的离散或非离散位点。 衬底可以由各种材料构成以形成二维或三维构型。 在优选的实施方案中，采用改性的光纤束或阵列作为基底以产生高密度阵列。 所公开的系统和方法具有检测目标分析物和筛选大型生物活性剂库的效用。

公开(公告)号：US20110195852A1

公开(公告)日：2011-08-11

申请号：US12675686

申请日：2007-08-30

Applicant: David R. Walt ,  David M. Rissin

Inventor： David R. Walt ,  David M. Rissin

IPC: C40B30/04

CPC classification number: C12Q1/40 ,  G01N33/54366 ,  G01N33/6854 ,  G01N2333/522 ,  G01N2333/938

Abstract: Disclosed is a method for measuring the concentration of an analyte or analytes in a solution. Although the methods can be conducted using a number of different assay formats, in one embodiment, the assays are conducted in reaction vessels defined, at least in part, by the distal ends of fiber optic strands.

Abstract translation: 公开了一种用于测量溶液中分析物或分析物的浓度的方法。 尽管可以使用许多不同测定形式进行该方法，但是在一个实施方案中，测定在至少部分由纤维光纤束的远端限定的反应容器中进行。

公开(公告)号：US20110089315A1

公开(公告)日：2011-04-21

申请号：US12664340

申请日：2008-06-25

Applicant: David R. Walt ,  Alexei R. Faustov

Inventor： David R. Walt ,  Alexei R. Faustov

IPC: H01S1/00

CPC classification number: G21K1/006 ,  G02B17/0615 ,  G02B17/0636

Abstract: Methods and devices are provided for the trapping, including optical trapping; analysis; and selective manipulation of particles on an optical array. A device parcels a light source into many points of light transmitted through a microlens optical array and an Offner relay to an objective, where particles may be trapped. Preferably the individual points of light are individually controllable through a light controlling device. Optical properties of the particles may be determined by interrogation with light focused through the optical array. The particles may be manipulated by immobilizing or releasing specific particles, separating types of particles, etc.

Abstract translation: 提供了捕获的方法和装置，包括光学捕获; 分析; 以及选择性地操纵光学阵列上的颗粒。 器件将光源包裹到通过微透镜光学阵列传输的许多光点中，并将光源继电器包裹到物镜，在该物镜上可能会捕获颗粒。 优选地，各个光点可以通过光控制装置单独控制。 颗粒的光学性质可以通过光聚焦的光学阵列询问来确定。 可以通过固定或释放特定颗粒，分离颗粒等来操纵颗粒。