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31.发明专利
公开(公告)号：JP2010145216A
公开(公告)日：2010-07-01
申请号：JP2008322199
申请日：2008-12-18
Inventor： YAMADA SEIJI , GOTO YOSHIO , TOKITA YUICHI
IPC: G01N27/327 , C12M1/40 , G01N27/416
CPC classification number: C07K17/14 , B82Y10/00 , G01N33/5438 , G01N2333/80 , G01N2610/00 , H01L51/0093 , H01L51/4206 , Y02E10/549 , Y02P70/521
Abstract: PROBLEM TO BE SOLVED: To provide a protein-immobilized electrode enabling stable use over a long period wherein highly stable cytochrome c552 is immobilized on a chemically stable gold electrode while the electron transport capability of the cytochrome is maintained, and a manufacturing method therefor. SOLUTION: A self-organizing monomolecular film 12 is formed on the gold electrode 11 using a hydrophobic thiol and a hydrophilic thiol. A protein-immobilized electrode wherein cytochrome c552 13 is immobilized on the gold electrode 11 with the self-organizing monomolecular film 12 interposed therebetween is produced by immersing, in a cytochrome c552 solution, the gold electrode 11 on which the self-organizing monomolecular film 12 is formed. COPYRIGHT: (C)2010,JPO&INPIT
Abstract translation: 待解决的问题：为了提供能够在长时间内稳定使用的蛋白质固定化电极，其中高度稳定的细胞色素c552固定在化学稳定的金电极上，同时保持细胞色素的电子传输能力，并且制造方法 为此。 解决方案：使用疏水性硫醇和亲水硫醇在金电极11上形成自组织单分子膜12。 通过在细胞色素c552溶液中浸渍自组织单分子膜12的金电极11，生成将细胞色素c552 13固定在金电极11上并插入自组织单分子膜12的蛋白质固定化电极 形成了。 版权所有（C）2010，JPO＆INPIT
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公开(公告)号：JP2009272179A
公开(公告)日：2009-11-19
申请号：JP2008122574
申请日：2008-05-08
Inventor： NAKAGAWA TAKAAKI , KUMITA HIDEYUKI , GOTO YOSHIO , SAKAI HIDEKI , TSUNODA MASAYA
CPC classification number: H01M8/16 , C12N9/0004 , H01M4/9008 , Y02E60/527
Abstract: PROBLEM TO BE SOLVED: To provide a new enzyme electrode that attains high output.
SOLUTION: There is provided an enzyme electrode that is an electrode, in which an oxidation-reduction reaction progresses using an enzyme as a catalyst. The enzyme electrode is configured to fix an enzyme modified so as to improve the affinity and/or a reaction rate with a reactive substrate or an electron transfer mediator, by adding or inserting at least one or more codons, coding a prescribed amino-acid residue, to a base sequence that codes the enzyme. The enzyme electrode is also configured for advancing an oxidation-reduction reaction on an electrode at an efficient rate, thereby realizing high output of electrical energy which can be obtained. For this reason, the enzyme electrode can be used suitably in all types of fuel cells, biosensors, and electronic apparatuses.
COPYRIGHT: (C)2010,JPO&INPITAbstract translation: 要解决的问题：提供一种达到高产量的新型酶电极。 解决方案：提供了酶电极，其是使用酶作为催化剂进行氧化还原反应的电极。 酶电极被配置为固定经修饰的酶，以通过加入或插入至少一个或多个密码子来改善与反应性底物或电子转移介质的亲和力和/或反应速率，编码规定的氨基酸残基 涉及编码酶的碱基序列。 酶电极也被配置为以有效的速率在电极上进行氧化还原反应，从而实现能够获得的高的电能输出。 因此，酶电极可以适用于所有类型的燃料电池，生物传感器和电子设备。 版权所有（C）2010，JPO＆INPIT
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公开(公告)号：JP2008289419A
公开(公告)日：2008-12-04
申请号：JP2007138643
申请日：2007-05-25
Inventor： TOKITA YUICHI , GOTO YOSHIO , SUGIYAMA HIROKI
CPC classification number: C12N9/0036 , C12Q1/32
Abstract: PROBLEM TO BE SOLVED: To provide a variant diaphorase whose heat resistance level is at a prescribed level or more.
SOLUTION: The variant diaphorase has a smaller potential energy than that of a wild type diaphorase at the formation of a complex with a coenzyme flavin mononucleotide. A method for screening the variant diaphorase having a smaller potential energy than that of a wild type diaphorase at the formation of a complex with a coenzyme flavin mononucleotide by molecular dynamics simulation is disclosed.
COPYRIGHT: (C)2009,JPO&INPITAbstract translation: 要解决的问题：提供耐热水平在规定水平以上的变形型心肌黄酶。 解决方案：与辅酶黄素单核苷酸形成复合物时，变体心肌黄蛋白酶具有比野生型心肌黄酶更小的势能。 公开了一种通过分子动力学模拟筛选与辅酶黄素单核苷酸形成复合物时具有比野生型心肌黄酶更小的势能的变体心肌黄酶的方法。 版权所有（C）2009，JPO＆INPIT
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公开(公告)号：JP2008289398A
公开(公告)日：2008-12-04
申请号：JP2007136820
申请日：2007-05-23
Inventor： TOKITA YUICHI , GOTO YOSHIO , SUGIYAMA HIROKI
CPC classification number: C12Q1/32 , C07K2299/00 , C12N9/0036
Abstract: PROBLEM TO BE SOLVED: To provide a variant diaphorase whose activity level is equal to or higher than a prescribed level.
SOLUTION: There provided is a variant diaphorase forming a three-dimensional structure in which a distance between tryptophane at the 60th site from the N-terminal and the first site imino nitrogen of a coenzyme flavin mononucleotide in a three-dimensional structure on the formation of a complex with the coenzyme flavin mononucleotide is larger than the distance in a three-dimensional structure by a wild type diaphorase. A method for screening the variant diaphorase forming a three-dimensional structure in which a distance between tryptophane at the 60th site from the N-terminal and the first site imino nitrogen of the coenzyme flavin mononucleotide in a three-dimensional structure on the formation of complex with the coenzyme flavin mononucleotide by a molecular dynamics simulation is larger than the distance in a three-dimensional structure by a wild type diaphorase.
COPYRIGHT: (C)2009,JPO&INPITAbstract translation: 要解决的问题：提供其活性水平等于或高于规定水平的变体心肌黄酶。 解决方案：提供了形成三维结构的变体心肌黄酶，其中在N-末端的第60位点的色氨酸与三维结构中辅酶黄素单核苷酸的第一位点亚氨基氮之间的距离在 与辅酶黄素单核苷酸的复合物的形成比野生型心肌黄蛋白酶在三维结构中的距离大。 筛选形成三维结构的变体心肌黄酶的方法，其中在N-末端的第60位点的色氨酸与辅酶黄素单核苷酸的第一位点亚氨基氮在三维结构中形成复合物的距离 通过分子动力学模拟，辅酶黄素单核苷酸大于野生型心肌黄蛋白酶在三维结构中的距离。 版权所有（C）2009，JPO＆INPIT
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公开(公告)号：JP2008161178A
公开(公告)日：2008-07-17
申请号：JP2007160964
申请日：2007-06-19
Inventor： KUMITA HIDEYUKI , TOKITA YUICHI , GOTO YOSHIO
CPC classification number: C12N9/001 , C12Y103/03005
Abstract: PROBLEM TO BE SOLVED: To provide a variant type bilirubin oxidase having at least a predetermined level of enzymic activity of bilirubin oxidase and its thermal resistance. SOLUTION: The variant type heat resistant bilirubin oxidase has an amino acid sequence of a wild type bilirubin oxidase originated from imperfect fungi, Myrothecium verrucaria, in which at least an amino acid residue is deleted, substituted, added or inserted so as to enhance the heat resistance. The variant type heat resistant bilirubin oxidase has denaturation temperature T m , for example, not lower than 72°C. COPYRIGHT: (C)2008,JPO&INPIT
Abstract translation: 要解决的问题：提供具有至少预定水平的胆红素氧化酶的酶活性及其耐热性的变体型胆红素氧化酶。 解决方案：变异型耐热性胆红素氧化酶具有源自不完全真菌的野生型胆红素氧化酶的氨基酸序列，其中至少一个氨基酸残基被缺失，取代，添加或插入，从而 提高耐热性。 变异型耐热性胆红素氧化酶具有变性温度T
m ，例如不低于72℃。 版权所有（C）2008，JPO＆INPIT -
Patent Agency Ranking36.发明专利Fuel cell and its manufacturing method, negative electrode for fuel cell and its manufacturing method, electronic equipment, electrode reaction utilization device and its manufacturing method, electrode for electrode reaction utilization device and its manufacturing method, high output agent, and mediator diffusion accelerator 审中-公开燃料电池及其制造方法，用于燃料电池的负极及其制造方法，电子设备，电极反应使用装置及其制造方法，电极反应用电极及其制造方法，高输出剂和中和剂扩散加速器
公开(公告)号：JP2007257983A
公开(公告)日：2007-10-04
申请号：JP2006080305
申请日：2006-03-23
Inventor： HATAKEYAMA TSUKASA , NAKAGAWA TAKAAKI , SAKAI HIDEKI , SATO ATSUSHI , TOKITA YUICHI , GOTO YOSHIO
CPC classification number: Y02E60/527 , Y02P70/56
Abstract: PROBLEM TO BE SOLVED: To provide a fuel cell capable of generating high output and its manufacturing method. SOLUTION: In the fuel cell having structure facing a negative electrode 1 and a positive electrode 2 through a proton conductor 3, and immobilizing enzyme, coenzyme, and mediator in the negative electrode 1, a high output agent comprising phospholipid such as dimyristoyl phosphatidylcholine (DMPC) or its derivative, or a polymer of them in addition to the enzyme, the coenzyme, and the mediator, or a mediator diffusion accelerator is immobilized to the negative electrode 1. As the mediator, a compound having quinone skeleton, 2-amino-1,4-naphtoquinone (ANQ) for example is used. COPYRIGHT: (C)2008,JPO&INPIT
Abstract translation: 要解决的问题：提供能够产生高输出的燃料电池及其制造方法。 解决方案：在具有面向负极1和通过质子导体3的正极2的结构的燃料电池中，并且在负极1中固定酶，辅酶和介体，包含磷脂的高输出剂如二肉豆蔻酰 磷脂酰胆碱（DMPC）或其衍生物或它们的除了酶，辅酶和介体或介体扩散促进剂的聚合物固定在负极1.作为介体，具有醌骨架的化合物2 - 氨基-1,4-萘醌（ANQ）。 版权所有（C）2008，JPO＆INPIT
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37.发明专利Microbial fuel cell, fuel and microbe for the fuel cell, and bioreactor and biosensor 审中-公开燃料电池，燃料和微生物燃料电池和生物制药和生物传感器的微生物燃料电池
公开(公告)号：JP2012190787A
公开(公告)日：2012-10-04
申请号：JP2012031405
申请日：2012-02-16
Inventor： SAKAI HIDEKI , MATSUMOTO RYUHEI , FUJITA SHUJI , GOTO YOSHIO , TOKITA YUICHI
CPC classification number: H01M8/16 , C12N9/0006 , C12N15/63 , Y02E60/527
Abstract: PROBLEM TO BE SOLVED: To provide a technique for increasing an output of a microbial fuel cell.SOLUTION: There is provided a microbial fuel cell using a polyol, such as glycerol, as fuel, and microbes, in which an enzyme catalyzing an oxidation-reduction reaction is introduced by genetic modification, on the negative electrode side. In the microbial fuel cell, a high output can be obtained by maintaining the microbes, in which an enzyme, such as diaphorase, catalyzing an oxidation-reduction reaction is introduced by genetic modification, on the negative electrode side, thereby accelerating a rate of the reaction.
Abstract translation: 要解决的问题：提供一种用于增加微生物燃料电池的输出的技术。 解决方案：在负极侧，提供使用多元醇，例如甘油作为燃料的微生物燃料电池和通过遗传修饰引入氧化还原反应的酶的微生物。 在微生物燃料电池中，通过维持在负极侧通过遗传修饰引入氧化还原反应的催化氧化还原反应的酶如氧化还原反应的微生物，可以获得高产量，从而加速 反应。 版权所有（C）2013，JPO＆INPIT
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38.发明专利Photoelectric conversion element, manufacturing method of photoelectric conversion element, solid state image sensor, manufacturing method of solid state image sensor, electronic apparatus, photoconductor, manufacturing method of photoconductor and multilayer transparent photoelectric conversion element 审中-公开光电转换元件，光电转换元件的制造方法，固态图像传感器，固态图像传感器的制造方法，电子设备，光电子器件，光电转换器和多层透明光电转换元件的制造方法
公开(公告)号：JP2012186320A
公开(公告)日：2012-09-27
申请号：JP2011048510
申请日：2011-03-07
Inventor： LUO WEI , TOKITA YUICHI , GOTO YOSHIO , YAMADA SEIJI , NAKAMARU AKIRA
IPC: H01L51/42 , H01L27/146
CPC classification number: H01L51/0093 , B82Y10/00 , H01L51/447 , Y02E10/549 , Y02P70/521
Abstract: PROBLEM TO BE SOLVED: To provide a photoconductor which can prevent the carriers excited by light from disappearing by recombination while enhancing photoelectric conversion efficiency, and to provide a photoelectric conversion element using the photoconductor.SOLUTION: The photoconductor is formed of a composite of a conductive polymer and/or a polymer semiconductor 11, and one or a plurality of proteins 12 containing at least one dye 12a having a long life excited state. The dye 12a becomes a luminescence center. The conductive polymer and/or a polymer semiconductor 11 is formed in network. The conductive polymer and/or a polymer semiconductor 11 and the protein 12 are bonded by non-covalent bond or covalent bond. A photoelectric conversion element is configured by connecting first and second electrodes electrically to different parts of the conductive polymer and/or a polymer semiconductor 11 in network of the photoconductor.
Abstract translation: 要解决的问题：提供一种光电导体，其可以防止由光激发的载流子在增强光电转换效率的同时消失，并提供使用光电导体的光电转换元件。 光电导体由导电聚合物和/或聚合物半导体11和包含至少一种具有长寿命激发态的染料12a的一种或多种蛋白质12的复合材料形成。 染料12a成为发光中心。 导电聚合物和/或聚合物半导体11形成在网络中。 导电聚合物和/或聚合物半导体11和蛋白质12通过非共价键或共价键键合。 光电转换元件通过将第一和第二电极电连接到光电导体网络中的导电聚合物和/或聚合物半导体11的不同部分而构成。 版权所有（C）2012，JPO＆INPIT
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39.发明专利
公开(公告)号：JP2011049435A
公开(公告)日：2011-03-10
申请号：JP2009197975
申请日：2009-08-28
Inventor： YAMADA SEIJI , LUO WEI , GOTO YOSHIO , TOKITA YUICHI
CPC classification number: H01L51/4253 , B82Y10/00 , H01L27/14601 , H01L31/101 , H01L51/0093 , Y02E10/549
Abstract: PROBLEM TO BE SOLVED: To provide a novel protein which has extremely high stability to the irradiation with light and has a long-lasting photoelectric conversion function, and also to provide a protein photoelectric conversion element which is stably used over a long period of time using the same. SOLUTION: Iron, which is a metal located at the center of a heme of horse heart cytochrome c, is substituted by tin to produce tin-substituted horse heart cytochrome c. Iron, which is a metal located at the center of a heme of bovine heart cytochrome c, is substituted by tin to produce tin-substituted bovine heart cytochrome c. A protein 22 comprising the tin-substituted horse heart cytochrome c or the tin-substituted bovine heart cytochrome c is immobilized on an electrode 21 to produce a protein-immobilized electrode. A protein photoelectric conversion element is formed by using the protein-immobilized electrode. COPYRIGHT: (C)2011,JPO&INPIT
Abstract translation: 要解决的问题：提供一种对光照射具有极高稳定性并具有持久的光电转换功能的新型蛋白质，并且还提供长期稳定使用的蛋白质光电转换元件 的时间使用相同。
解决方案：铁是位于马心细胞色素c血红素中心的金属，被锡取代，产生锡取代的马心细胞色素c。 位于牛心脏细胞色素c血红素中心的金属铁被锡取代，产生锡取代的牛心脏细胞色素c。 包含锡取代的马心脏细胞色素c或锡取代的牛心脏细胞色素c的蛋白质22被固定在电极21上以产生蛋白质固定的电极。 通过使用蛋白质固定化电极形成蛋白质光电转换元件。 版权所有（C）2011，JPO＆INPIT
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公开(公告)号：JP2009044997A
公开(公告)日：2009-03-05
申请号：JP2007213512
申请日：2007-08-20
Inventor： SUGIYAMA HIROKI , GOTO YOSHIO , TOKITA YUICHI
CPC classification number: C12N9/96 , C12N9/001 , H01M4/9008 , H01M8/16 , Y02E60/527
Abstract: PROBLEM TO BE SOLVED: To provide a new method for coating BOD (bilirubin oxidase).
SOLUTION: Provided is the BOD coating method for carrying out a polymerizable functional group introducing step of introducing the polymerizable functional group into the surface of the BOD molecule, and a copolymerizing step of initiating copolymerization of the polymerizable functional group with a polymerizable monomer at ≤17°C. The stability to heat and organic solvents can be improved while suppressing deterioration of the enzyme activity of the coated BOD by using the method for coating. Furthermore, the BOD coated by using the method can suitably be used for an enzyme electrode of a fuel cell.
COPYRIGHT: (C)2009,JPO&INPITAbstract translation: 要解决的问题：提供一种涂覆BOD（胆红素氧化酶）的新方法。 解决方案：提供了将可聚合官能团引入到BOD分子的表面中的可聚合官能团引入步骤的BOD涂覆方法，以及引发可聚合官能团与可聚合单体的共聚的共聚步骤 在≤17℃。 通过使用涂布方法，能够抑制涂布的BOD的酶活性的劣化，可以提高对热和有机溶剂的稳定性。 此外，通过使用该方法涂布的BOD可以适当地用于燃料电池的酶电极。 版权所有（C）2009，JPO＆INPIT
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