公开(公告)号：JP2014209898A

公开(公告)日：2014-11-13

申请号：JP2013123003

申请日：2013-06-11

Applicant: 東ソー株式会社 ,  Tosoh Corp

Inventor： SATO HIROSHI ,  NOGUCHI ATSUSHI ,  NAKANISHI MUTSUMI ,  MAKINO YURIKO ,  ICHII TAKASHI ,  IDE TERUHIKO

IPC: C12N15/09 ,  C12N1/15 ,  C12N9/10

CPC classification number: C12N9/1276

Abstract: 【課題】熱安定性が向上したトリ骨髄芽細胞腫ウイルス(AMV)逆転写酵素、およびその製造方法を提供すること。【解決の手段】 AMV逆転写酵素を構成するアミノ酸のうち、特定の位置にあるアミノ酸を他のアミノ酸に置換することにより、熱安定性が向上したAMV逆転写酵素を得ることができた。また前記逆転写酵素をコードするポリヌクレオチドを含む発現ベクターで宿主を形質転換して得られる形質転換体を培養することで、前記逆転写酵素を製造することができた。【選択図】図4

Abstract translation: 要解决的问题：提供具有改善的热稳定性的禽成骨细胞瘤病毒（AMV）逆转录酶，并提供其制备方法。解决方案：具有改善的热稳定性的AMV逆转录酶可以通过在 构成AMV逆转录酶的氨基酸与其他氨基酸的特异性位置。 逆转录酶可以通过培养通过用包含编码逆转录酶的多核苷酸的表达载体转化宿主获得的转化体来产生。

公开(公告)号：JP2013146235A

公开(公告)日：2013-08-01

申请号：JP2012009959

申请日：2012-01-20

Applicant: Tosoh Corp ,  東ソー株式会社

Inventor： SATO HIROSHI ,  NOGUCHI JUN ,  UNE KURATO ,  IDE TERUHIKO

IPC: C12N9/12 ,  C12N1/21 ,  C12N15/09 ,  C12R1/19

Abstract: PROBLEM TO BE SOLVED: To provide a host capable of expressing an avian myeloblastoma virus (AMV) reverse transcriptase in a large amount by using a transformant, in producing the AMV reverse transcriptase by using the transformant obtained by transforming the host by using a vector containing an AMV reverse transcriptase gene.SOLUTION: The AMV reverse transcriptase is expressed in a large amount by using either one of strains of the E.coli MV1184 strain, the E.coli W3110 strain, the E.coli GM31 strain, the E.coli HB101 strain and the E.coli JM101 strain as a host.

Abstract translation: 要解决的问题：为了通过使用转化体提供能够大量表达禽成骨细胞瘤病毒（AMV）逆转录酶的宿主，通过使用通过使用通过使用包含以下的载体转化宿主获得的转化体来产生AMV逆转录酶 AMV逆转录酶基因。解决方案：通过使用大肠杆菌MV1184菌株，大肠杆菌W3110菌株，大肠杆菌GM31菌株，大肠杆菌中的任一种菌株，大量表达AMV逆转录酶 HB101菌株和大肠杆菌JM101菌株作为宿主。

公开(公告)号：JP2012139164A

公开(公告)日：2012-07-26

申请号：JP2010294127

申请日：2010-12-28

Applicant: Tosoh Corp ,  東ソー株式会社

Inventor： OE MASATAKE ,  NOGUCHI JUN ,  IDE TERUHIKO

IPC: C12P31/00

Abstract: PROBLEM TO BE SOLVED: To provide an efficient method for producing carotenoid, especially carotenes such as lycopene, etc., by using microorganisms.SOLUTION: The method for producing the carotenoid is provided by using a medium added with calcium compound so that its final concentration becomes ≥3.6 mM and culturing the microorganisms having a carotenoid-producing ability by using the medium.

Abstract translation: 要解决的问题：提供通过使用微生物生产类胡萝卜素，特别是胡萝卜素如番茄红素等的有效方法。 解决方案：通过使用添加有钙化合物的培养基来提供类胡萝卜素的制备方法，使其最终浓度变为≥3.6mM，并通过使用培养基培养具有类胡萝卜素生产能力的微生物。 版权所有（C）2012，JPO＆INPIT

公开(公告)号：JP2012072091A

公开(公告)日：2012-04-12

申请号：JP2010218965

申请日：2010-09-29

Applicant: Tosoh Corp ,  東ソー株式会社

Inventor： TANAKA TORU ,  ASAOKA YOSHIHARU ,  IDE TERUHIKO

IPC: C07K1/22 ,  C07K17/00

Abstract: PROBLEM TO BE SOLVED: To provide a method for purifying an antibody to a high purity by affinity chromatography using, as a ligand, a protein (Fc binding protein) that highly recognizes an Fc region of the antibody.SOLUTION: The method for purifying the antibody comprises steps of: adding a solution containing the antibody to a solid phase on which the Fc binding protein is immobilized, so as to achieve adsorption of the antibody onto the solid phase; and eluting the antibody adsorbed onto the solid phase using an eluate having pH of ≤3.5.

Abstract translation: 要解决的问题：提供一种通过亲和层析纯化抗体的方法，所述亲和层析使用高度识别抗体Fc区的蛋白质（Fc结合蛋白）作为配体。 解决方案：纯化抗体的方法包括以下步骤：将含有抗体的溶液加入其上固定有Fc结合蛋白的固相，以实现抗体吸附在固相上; 并使用pH为≤3.5的洗脱液洗脱吸附在固相上的抗体。 版权所有（C）2012，JPO＆INPIT

公开(公告)号：JP2011072246A

公开(公告)日：2011-04-14

申请号：JP2009226613

申请日：2009-09-30

Applicant: Tosoh Corp ,  東ソー株式会社

Inventor： ASAOKA YOSHIHARU ,  IDE TERUHIKO

IPC: C12N15/09 ,  C12N1/19 ,  C12P21/02 ,  C12R1/645

Abstract: PROBLEM TO BE SOLVED: To provide a plasmid capable of expressing a nearly natural human Fc receptor, FcγRI, using a gene engineering procedure, a eukaryote obtained by transforming the plasmid, and a method for producing human FcγRI by using the eukaryote.
SOLUTION: There are disclosed an expression plasmid including a polynucleotide encoding a soluble human Fc receptor FcγRI, yeast capable of expressing a soluble human Fc receptor FcγRI which is obtained by transforming yeast by introducing the plasmid, and a method for producing human Fc receptor FcγRI using the yeast.
COPYRIGHT: (C)2011,JPO&INPIT

Abstract translation: 待解决的问题：使用基因工程程序提供能够表达几乎天然人Fc受体的FcγRI质粒，通过转化质粒得到的真核生物，以及使用真核生物制备人FcγRI的方法。 解决方案：公开了一种表达质粒，其包含编码可溶性人Fc受体FcγRI的多核苷酸，能够表达可通过引入质粒转化酵母得到的可溶性人Fc受体FcγRI的酵母，以及生产人Fc 受体FcγRI。 版权所有（C）2011，JPO＆INPIT

公开(公告)号：JP2010236975A

公开(公告)日：2010-10-21

申请号：JP2009084083

申请日：2009-03-31

Applicant: Sagami Chemical Research Institute ,  Tosoh Corp ,  公益財団法人相模中央化学研究所 ,  東ソー株式会社

Inventor： WATABE KAZUO ,  IJUIN YASUJI ,  IDE TERUHIKO

IPC: G01N30/88 ,  C08J3/14

Abstract: PROBLEM TO BE SOLVED: To provide a method for producing microbial cellulose particles usable as a separating material for chromatography, and the separating material for chromatography obtained by the producing method. SOLUTION: This method for producing microbial cellulose particles includes processes for: dissolving the microbial cellulose into alkali aqueous solution; granulating the microbial cellulose after adding a dispersion solvent into dissolved liquid; freezing the microbial cellulose by adding the granulated microbial cellulose into a cooled freezing solvent; and cleaning the frozen microbial cellulose with a cleaning solvent. The cellulose particles obtained by the producing method are also provided. COPYRIGHT: (C)2011,JPO&INPIT

Abstract translation: 待解决的问题：提供可用作色谱分离材料的微生物纤维素颗粒的制备方法和通过该制备方法获得的色谱分离材料。 解决方案：这种生产微生物纤维素颗粒的方法包括：将微生物纤维素溶解在碱性水溶液中的方法; 在分散溶剂中加入溶解液后对微生物纤维素进行造粒; 通过将颗粒状微生物纤维素加入到冷冻的冷冻溶剂中来冷冻微生物纤维素; 并用清洁溶剂清洗冷冻的微生物纤维素。 还提供了通过制备方法获得的纤维素颗粒。 版权所有（C）2011，JPO＆INPIT

公开(公告)号：JP2010126436A

公开(公告)日：2010-06-10

申请号：JP2008299094

申请日：2008-11-25

Applicant: Tosoh Corp ,  東ソー株式会社

Inventor： TANAKA TORU ,  IDE TERUHIKO ,  IIDA HIROSHI

IPC: C07K16/00 ,  B01J20/24 ,  C07K1/22 ,  C12N15/09

Abstract: PROBLEM TO BE SOLVED: To provide an adsorbent for purifying an antibody making use of a human Fc receptor FcγRI, and a method of purifying an antibody using the same.
SOLUTION: The adsorbent includes the FcγRI produced by using a CHO cell that is obtained by the transformation of an expression plasmid comprising a DNA sequence encoding a soluble human Fc receptor FcγRI and stably expresses the human Fc receptor FcγRI, with the FcγRI fixed onto a support. The method of purifying an antibody employs the adsorbent.
COPYRIGHT: (C)2010,JPO&INPIT

Abstract translation: 待解决的问题：提供用于纯化使用人Fc受体FcγRI的抗体的吸附剂，以及使用其的纯化抗体的方法。 溶液：吸附剂包括通过使用CHO细胞产生的FcγRI，其通过转化包含编码可溶性人Fc受体FcγRI的DNA序列并稳定表达人Fc受体FcγRI的表达质粒而获得，FcγRI固定 支持。 纯化抗体的方法采用吸附剂。 版权所有（C）2010，JPO＆INPIT

公开(公告)号：JP2006280297A

公开(公告)日：2006-10-19

申请号：JP2005106045

申请日：2005-04-01

Applicant: Tosoh Corp ,  東ソー株式会社

Inventor： IDE TERUHIKO ,  TANAKA TORU

IPC: C12N15/09 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N9/00 ,  C12P23/00

CPC classification number: Y02P20/52

Abstract: PROBLEM TO BE SOLVED: To provide a new geranyl geranyl diphosphate synthetase, and a gene encoding the synthetase. SOLUTION: The protein having a specific amino acid sequence or an amino acid sequence having mutation induced thereto, and having geranyl geranyl diphosphate synthetase activities, the gene encoding the synthetase, the expression vector containing the gene are provided. The method or the like for culturing a host cell transformed by the expression vector, and collecting the geranyl geranyl diphosphate synthetase and carotenoids from the cultured product is also provided. COPYRIGHT: (C)2007,JPO&INPIT

Abstract translation: 要解决的问题：提供新的香叶基香叶基二磷酸合成酶和编码合成酶的基因。 解决方案：提供具有特异性氨基酸序列或具有诱导突变的氨基酸序列并具有香叶基香叶基二磷酸合成酶活性的蛋白质，编码合成酶的基因，含有该基因的表达载体。 还提供了用于培养由表达载体转化的宿主细胞，以及从培养产物收集香叶基香叶基二磷酸合成酶和类胡萝卜素的方法等。 版权所有（C）2007，JPO＆INPIT

公开(公告)号：JP2004217573A

公开(公告)日：2004-08-05

申请号：JP2003007424

申请日：2003-01-15

Applicant: Marine Biotechnol Inst Co Ltd ,  Tosoh Corp ,  東ソー株式会社 ,  株式会社海洋バイオテクノロジー研究所

Inventor： IDE TERUHIKO

IPC: C07K14/005 ,  C07K16/00

Abstract: PROBLEM TO BE SOLVED: To provide a substance high in binding specificity to immunoglobulin and extremely slight in the deterioration of its binding characteristics in undergoing sterilization treatment or during storage.
SOLUTION: The immunoglobulin-binding peptide is represented by the following amino acid sequences(I), (II), (III) or (IV) or the like: (I) Arg-Ser-Thr-Leu-X1-X2-X3-Leu( wherein, X1 is Pro, Thr or Gln; X2 is Pro, Ala or Ile; and X3 is Ser or Gly ). (II) Ser-Gln-Ser-X4-Pro( wherein, X4 is Glu or Arg ). (III) Leu-X5-Gln-Pro-Leu( wherein, X5 is Ser, Leu or Val ). (IV) His-Leu-X6-X7-Ala-X8-X9-Ser( wherein, X6 is Pro or Arg; X7 is Thr or Lys; X8 is His or Leu; and X9 is Ala or Ser ). A carrier for refining immunoglobulin is such as to use the peptide, and a method for refining immunoglobulin involves using the peptide.
COPYRIGHT: (C)2004,JPO&NCIPI

公开(公告)号：JP2001139600A

公开(公告)日：2001-05-22

申请号：JP32511799

申请日：1999-11-16

Applicant: TOSOH CORP

Inventor： IDE TERUHIKO ,  TSUCHIYA SHIGEO ,  YASUKAWA KIYOSHI ,  ISHIGURO NORIHIKO

IPC: C12P21/02 ,  C07K1/18 ,  C07K14/54 ,  C07K14/715 ,  C07K19/00

Abstract: PROBLEM TO BE SOLVED: To provide a method for purifying a fused protein of IL-6R and IL-6 in industrial scale and high purity without causing denaturation and deactivation. SOLUTION: This method for purifying a fused protein of IL-6R with IL-6 comprises bringing a solution containing the fused protein of IL-6R with IL-6 into contact with absorbent particles floating in a fluid bed, feeding the eluent and eluting and recovering absorbed fraction to the adsorbent particles.