公开(公告)号：EP2235736A4

公开(公告)日：2011-09-07

申请号：EP08859342

申请日：2008-12-15

Applicant: BIOVIGILANT SYSTEMS INC

Inventor： BINNIE ERIK H ,  MORRIS GREGORY SCOTT

IPC: H01J40/14

CPC classification number: G01N15/1434 ,  G01N1/2247 ,  G01N1/2273 ,  G01N15/1404 ,  G01N15/1459 ,  G01N21/53 ,  G01N21/6486 ,  G01N2015/0088 ,  G01N2015/025 ,  G01N2015/1486 ,  G01N2015/1493 ,  G01N2201/06113 ,  G01N2201/06193

公开(公告)号：EP2027250A2

公开(公告)日：2009-02-25

申请号：EP07797660.3

申请日：2007-05-22

Applicant: Lumencor, Inc.

Inventor： JAFFE, Claudia B. ,  JAFFE, Steven M. ,  JONES, Michieal L.

IPC: C12M1/34

CPC classification number: G01J3/10 ,  G01N21/253 ,  G01N21/255 ,  G01N21/4788 ,  G01N21/51 ,  G01N21/53 ,  G01N21/6452 ,  G01N2021/6419 ,  G01N2021/6469 ,  G01N2021/6484 ,  G01N2201/06193

Abstract: The invention relates to a light source for irradiating molecules present in a detection volume with one or more selected wavelengths of light and directing the fluorescence, absorbance, transmittance, scattering onto one or more detectors. Molecular interactions with the light allow for the identification and quantitation of participating chemical moieties in reactions utilizing physical or chemical tags, most typically fluorescent and chromophore labels. The invention can also use the light source to separately and simultaneously irradiate a plurality of capillaries or other flow confining structures with one or more selected wavelengths of light and separately and simultaneously detect fluorescence produced within the capillaries or other flow confining structures. In various embodiments, the flow confining structures can allow separation or transportation of molecules and include capillary, micro bore and milli bore flow systems. The capillaries are used to separate molecules that are chemically tagged with appropriate fluorescent or chromophore groups.

公开(公告)号：JP6215126B2

公开(公告)日：2017-10-18

申请号：JP2014087665

申请日：2014-04-21

Applicant: クラウディオ・オリベイラ・エガロン

Inventor： クラウディオ・オリベイラ・エガロン

IPC: G01N21/64 ,  G01N21/78 ,  G01N21/01

CPC classification number: G01N21/251 ,  G01D5/353 ,  G01D5/35345 ,  G01F23/2927 ,  G01N21/17 ,  G01N21/64 ,  G01N21/645 ,  G01N21/648 ,  G01N21/76 ,  G02B6/00 ,  G01D5/35387 ,  G01N2021/635 ,  G01N2021/6484 ,  G01N2021/7736 ,  G01N21/7703 ,  G01N2201/06193 ,  G01N2201/062 ,  G01N2201/088

公开(公告)号：JP2011506977A

公开(公告)日：2011-03-03

申请号：JP2010538231

申请日：2008-12-15

Applicant: バイオビジラント システムズ,インコーポレイテッド

Inventor： ビニー，エリック，エイチ． ,  モリス，グレゴリー，スコット

IPC: G01N15/14 ,  G01N21/64

CPC classification number: G01N15/1434 ,  G01N1/2247 ,  G01N1/2273 ,  G01N15/1404 ,  G01N15/1459 ,  G01N21/53 ,  G01N21/6486 ,  G01N2015/0088 ,  G01N2015/025 ,  G01N2015/1486 ,  G01N2015/1493 ,  G01N2201/06113 ,  G01N2201/06193

Abstract: A method and apparatus for detecting pathogens and particles in a fluid in which particle size and intrinsic fluorescence of a simple particle is determined, comprising a sample cell; a light source on one side of the sample cell for sending a focused beam of light through the sample, whereby portions of the beam of light are scattered at various angles by particles of various sizes present in the sample area; a particle size detector positioned in the light path for detecting a portion of forward scattered light; a pair of fluorescence detectors positioned off axis from the beam of light; and a pair of elliptical mirrors positioned such that an intersection of the incoming particle stream and the light beam are at one foci of each ellipsoid, and one of said pair of fluorescence detectors lies at the other foci.

公开(公告)号：KR1020110005677A

公开(公告)日：2011-01-18

申请号：KR1020107015547

申请日：2008-12-15

Applicant: 아즈빌주식회사

Inventor： 비니에릭에이치 ,  모리스그레고리스캇

IPC: G01N33/52 ,  C12Q1/04 ,  C12M1/34 ,  H01J40/14

CPC classification number: G01N15/1434 ,  G01N1/2247 ,  G01N1/2273 ,  G01N15/1404 ,  G01N15/1459 ,  G01N21/53 ,  G01N21/6486 ,  G01N2015/0088 ,  G01N2015/025 ,  G01N2015/1486 ,  G01N2015/1493 ,  G01N2201/06113 ,  G01N2201/06193 ,  G01N33/52 ,  C12M1/34 ,  C12Q1/04 ,  G01N33/483 ,  H01J40/14

Abstract: 본 발명은 단일 미립자의 미립자 크기와 고유의 형광이 결정되는, 유체 중의 병원균 및 미립자를 검출하기 위한 방법 및 장치에 관한 것으로서, 시료 셀과, 집속된 광 빔을 시료를 통해 전달하도록 시료 셀의 일측에 있는 광원으로서, 광 빔의 일부는 시료 영역에 존재하는 다양한 크기의 미립자에 의해 다양한 각도로 산란되는 것인 광원과, 광 경로에 배치되어, 전방을 향해 산란된 광의 일부를 검출하기 위한 미립자 크기 검출기와, 광 빔으로부터 편축에 배치되는 한쌍의 형광 검출기와, 유입되는 미립자 스트림과 광 빔의 교차점이 각 타원면의 한 초점에 있고 한쌍의 형광 검출기 중 하나가 다른 초점에 있도록 배치되는 한쌍의 타원형 미러를 포함한다.

公开(公告)号：JP2016505263A

公开(公告)日：2016-02-25

申请号：JP2015551819

申请日：2014-01-07

Applicant: ザ ブリガム アンド ウィメンズ ホスピタル インコーポレイテッドThe Brigham and Women’s Hospital, Inc. ,  ザ ブリガム アンド ウィメンズ ホスピタル インコーポレイテッドThe Brigham and Women’s Hospital, Inc. ,  プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ

Inventor： キャサリン ラコウスキ ,  キャサリン ラコウスキ ,  マンキ デン ,  マンキ デン ,  ダニエル ニードルマン ,  ダニエル ニードルマン

IPC: C12Q1/04 ,  G01N21/64

CPC classification number: G01N21/6408 ,  G01N21/6458 ,  G01N21/6486 ,  G01N2201/06193

Abstract: 本発明は、例えば生殖補助医療における卵母細胞および胚の評価において用いられ得る、蛍光寿命イメージング顕微鏡を用いて卵母細胞および/または胚の代謝状態を評価するための新規な非侵襲的インビトロ方法を提供する。

Abstract translation: 另外，本发明，例如，可以在卵母细胞和在辅助生殖技术胚胎的评估中使用的，新的非侵入性的体外方法，用于通过使用荧光寿命成像显微术评估卵母细胞和/或胚胎的代谢状态 提供。

公开(公告)号：JP5695089B2

公开(公告)日：2015-04-01

申请号：JP2012550448

申请日：2011-01-27

Applicant: エフ.ホフマン−ラ ロシュ アーゲー ,  F. HOFFMANN−LA ROCHE AKTIENGESELLSCHAFT

Inventor： ヨアヒム ヘーネス ,  フレデリック ウェオウスキ ,  ラインホルト ミシュラー ,  ヘルベルト ハルティッヒ

IPC: G01N33/52 ,  G01N21/64 ,  G01N33/66

CPC classification number: G01N21/3151 ,  G01N21/8483 ,  G01N2021/3155 ,  G01N2201/06193 ,  G01N2201/0696

公开(公告)号：US20240328946A1

公开(公告)日：2024-10-03

申请号：US18267586

申请日：2022-10-13

Applicant: Xiamen University

Inventor： Donghui LI ,  Yan ZHANG ,  Ping HUANG ,  Yabin DENG

IPC: G01N21/64

CPC classification number: G01N21/643 ,  G01N2201/06193 ,  G01N2201/127

Abstract: The present disclosure provides a fluorescence analysis method for lithium ion determination using free-base phthalocyanine (FBPc) as a molecular probe, and relates to the technical field of fluorescent probes. The method includes the following steps: adding an alkaline organic medium separately into a plurality of reaction vessels, and adding a phthalocyanine organic solution having a same volume as that of the alkaline organic medium; adding lithium ion organic solutions with increasing concentrations in sequence; diluting an obtained reaction system, allowing to stand to conduct a reaction, scanning a fluorescence spectrum of the reaction system, and determining a relative fluorescence intensity at a fluorescence peak. A determination principle is that in organic media, especially an alkaline organic medium, lithium ions can react with the FBPc to emit strong red fluorescence, and generation of the fluorescence has the remarkable characteristics of ultra-sensitivity and high specificity.

公开(公告)号：US20170322151A1

公开(公告)日：2017-11-09

申请号：US15520293

申请日：2015-10-05

Applicant: VYSOKE UCENI TECHNICKE V BRNE

Inventor： Pavel KOLMAN ,  Radim CHMELIK

IPC: G01N21/45 ,  G01N21/64 ,  G02B21/00

CPC classification number: G01N21/453 ,  G01N21/45 ,  G01N21/64 ,  G01N21/6456 ,  G01N21/6458 ,  G01N2201/06193 ,  G02B21/00 ,  G02B21/14 ,  G03H1/0443 ,  G03H2001/0467 ,  G03H2222/14 ,  G03H2222/24 ,  G03H2223/23

Abstract: An interferometric system and a method of measurement of refractive index spatial distribution for use in digital holographic microscopy to observe samples in reflected as well as transmitted radiation or to observe luminescent samples comprises a first branch and a second branch with a plurality of optical elements. The first branch comprises a diffraction grating located in a plane optically conjugated with the object plane in order to create an achromatic hologram with spatial carrier frequency in the output image plane.

公开(公告)号：US20160161410A1

公开(公告)日：2016-06-09

申请号：US15040545

申请日：2016-02-10

Applicant: Howard Hughes Medical Institute

Inventor： Philipp Johannes Keller

IPC: G01N21/64 ,  G02B21/16 ,  G02B27/10 ,  G02B21/00 ,  G02B21/36 ,  G01N33/483 ,  G02B21/06

CPC classification number: G01N21/6486 ,  A61B5/0071 ,  A61B2503/42 ,  G01N21/64 ,  G01N33/4833 ,  G01N2021/1736 ,  G01N2201/06193 ,  G01N2201/064 ,  G02B21/0004 ,  G02B21/0048 ,  G02B21/06 ,  G02B21/16 ,  G02B21/361 ,  G02B21/362 ,  G02B21/367 ,  G02B27/1013

Abstract: A live biological specimen is imaged by generating a plurality of light sheets; directing the plurality of light sheets along an illumination axis through the biological specimen such that the light sheets spatially and temporally overlap within the biological specimen along an image plane, and optically interact with the biological specimen within the image plane; and recording, at each of a plurality of views, images of the fluorescence emitted along a detection axis from the biological specimen due to the optical interaction between the light sheets and the biological specimen. The temporal overlap is within a time shift that is less than a resolution time that corresponds to a spatial resolution limit of the microscope.

Abstract translation: 通过生成多个光片对活体生物样本进行成像; 沿着照明轴线引导多个光片通过生物样本，使得光片沿着图像平面在空间和时间上在生物样本内重叠，并与图像平面内的生物样本光学相互作用; 并且在多个视图的每一个处，由于光片和生物样本之间的光学相互作用，沿着检测轴从生物样本记录荧光的图像。 时间重叠在时间偏移之内，其小于对应于显微镜的空间分辨率极限的分辨率时间。