公开(公告)号：US20020076729A1

公开(公告)日：2002-06-20

申请号：US09826682

申请日：2001-04-05

Inventor： Tobias Meyer ,  Mary N. Teruel

IPC: G01N033/53 ,  G01N033/567 ,  C12M001/34 ,  G01J003/00

CPC classification number: G01N21/648 ,  G01N21/552 ,  G01N21/6428 ,  G01N21/6456

Abstract: An apparatus for screening for translocation of a first protein of interest in vivo in a plurality of cells comprises (a) a thin unitary total internal reflection member having a surface portion, (b) a plurality cell contacted to said surface portion by the plasma membrane of said cell, said cell containing said first protein of interest, the protein of interest having a fluorescent group conjugated thereto; (c) a light source operatively associated with the total internal reflection member and positioned for directing a source light into the member to produce an evanescent field adjacent the surface portion, with the evanescent field extending into a first portion of the cell adjacent the plasma membrane, the evanescent field being weaker in a second portion of the cell, the fluorescent group emitting light when in the first portion of the cell and emitting less light when in the second portion of the cell; (d) coupling means for coupling the light source to the thin unitary total internal reflection member and illuminate at least 10 square millimeters of the surface portion; and (e) a light detector operatively associated with the total internal reflection member and configured to detect emitted light from the cells, whereby the emission of more or less light from the cell indicates the translocation of the protein between the first and second portions of the cell.

Abstract translation: 用于筛选多个细胞中体内第一种感兴趣蛋白质易位的装置包括（a）具有表面部分的薄单一全内反射构件，（b）通过质膜与所述表面部分接触的多个单元 的所述细胞，所述细胞含有所述第一目的蛋白质，所述目的蛋白质具有与其缀合的荧光基团; （c）与全内反射构件可操作地相关联并定位成用于将源光引导到构件中以产生邻近表面部分的ev逝场的光源，其中渐逝场延伸到邻近质膜的单元的第一部分 在细胞的第二部分中，消逝场较弱，荧光组在细胞的第一部分中发光并且在细胞的第二部分中发光较少; （d）耦合装置，用于将光源耦合到薄的整体式全反射构件并照亮表面部分的至少10平方毫米; 和（e）光检测器，其与全内反射构件可操作地相关联并且被配置为检测来自细胞的发射光，由此来自细胞的更多或更少的光的发射指示蛋白质在第一和第二部分之间的易位 细胞。

公开(公告)号：US20020012119A1

公开(公告)日：2002-01-31

申请号：US09828166

申请日：2001-04-09

Inventor： Sylvie Menezo ,  Franck Delorme

IPC: G01J003/00

CPC classification number: G02B6/12019

Abstract: The invention relates to a device for making a comparison between N nominal wavelength values (nullei) and the wavelength values of optical signals coupled respectively to input ports (GE1) of a phasar whose structure comprises a grating (R) of guides (Gj) According to the invention, the phasar comprises two monitoring output ports (A,B) respectively placed about focal points corresponding to two interference orders of the grating (R) designed to collect the combination of the said signals, and its structure is designed so that the transmission function approximates to a triangular shape in order to widen the monitoring span. The invention applies notably to the wavelength 15 monitoring of a tuneable optical source.

Abstract translation: 本发明涉及一种用于比较N个标称波长值（lambdei）和分别耦合到其结构包括导向器（Gj）的光栅（R））的相柱的输入端口（GE1）的光信号的波长值的装置。 对于本发明，相柱包括分别放置在对应于设计成收集所述信号的组合的光栅（R）的两个干涉阶数的焦点周围的两个监视输出端口（A，B），并且其结构被设计成使得 传输函数近似于三角形，以扩大监测范围。 本发明特别适用于可调谐光源的波长15监视。

公开(公告)号：US20040252300A1

公开(公告)日：2004-12-16

申请号：US10460101

申请日：2003-06-12

Inventor： Richard C. Slater

IPC: G01J003/30 ,  G01J003/00

CPC classification number: G01N21/35 ,  G01J3/42 ,  G01N21/3581 ,  G01N2021/1793

Abstract: A light source produces a pump wave that is used to pump a parametric device. The parametric device is configured at or near its degeneracy point and produces a broadband output. The broadband output is directed to a remote location where chemical agents may be located. The broadband output may be transmitted through or scattered from the remote location and chemical agents located at the remote location may absorb portions of the broadband output. The broadband output may be collected and dispersed to produce channels or sub-bands that are detected by a detector array. The detector array may multiplex the intensities of the sub-bands and produce an absorption spectrum. The absorption spectrum may be compared to a library of known chemical agents and the presence of a chemical agent at the remote location can be determined in or near real time.

Abstract translation: 光源产生用于泵浦参数设备的泵浦波。 参数化设备配置在其退化点处或附近，并产生宽带输出。 宽带输出定向到化学试剂可能位于的远程位置。 宽带输出可以从远程位置传输或散布，位于远程位置的化学剂可以吸收宽带输出的部分。 可以收集和分散宽带输出以产生由检测器阵列检测的通道或子带。 检测器阵列可以复用子带的强度并产生吸收光谱。 可以将吸收光谱与已知化学试剂的文库进行比较，并且可以实时或接近实时地确定在远程位置处的化学试剂的存在。

公开(公告)号：US20040246477A1

公开(公告)日：2004-12-09

申请号：US10800320

申请日：2004-03-11

Inventor： John A. Moon ,  James S. Sirkis ,  Ralph Jones ,  Charles R. Winston ,  David R. Fournier ,  Joseph Pinto ,  Robert N. Brucato ,  James R. Dunphy ,  Christopher J. Chestnut

IPC: G01J003/00 ,  G01J003/42

CPC classification number: G01J3/06 ,  G01J3/02 ,  G01J3/0202 ,  G01J3/0208 ,  G01J3/021 ,  G01J3/0218 ,  G01J3/0256 ,  G01J3/0291 ,  G01J3/18 ,  G01J3/28 ,  G01J3/32 ,  G01J3/36 ,  G01J2003/064 ,  G01J2003/1819 ,  G02B6/2931 ,  G02B6/4215 ,  H04B10/077 ,  H04B10/07955 ,  H04Q2011/0026 ,  H04Q2011/0049 ,  H04Q2011/0083

Abstract: An optical spectrum analyzer (OSA) 10 sequentially or selectively samples (or filters) a spectral band(s) 11 of light from a broadband optical input signal 12 and measures predetermined optical parameters of the optical signal (e.g., spectral profile) of the input light 12. The OSA 10 is a free-space optical device that includes a collimator assembly 15, a diffraction grating 20 and a mirror 22. A launch pigtail emits into free space the input signal through the collimator assembly 15 and onto the diffraction grating 20, which separates or spreads spatially the collimated input light, and reflects the dispersed light onto the mirror 22. A null/4 plate 26 is disposed between the mirror 22 and the diffraction grating 20. The mirror reflects the separated light back through the null/4 plate 26 to the diffraction grating 20, which reflects the light back through the collimating lens 18. The lens 18 focuses spectral bands of light (null1-nullN) at different focal points in space. One of the spectral bands 11 is focused onto a receive pigtail 28, which then propagates to a photodetector 30. A pivoting mechanism 34 pivots the diffraction grating 20 or mirror 22 about a pivot point 36 to sequentially or selectively focus each spectral band 11 to the receive pigtail 28. A position sensor 42 detects the displacement of the diffraction grating 24 or mirror.

Abstract translation: 光谱分析仪（OSA）10顺序地或选择性地从宽带光输入信号12采样（或滤波）光谱带11，并且测量输入的光信号的光学参数（例如，光谱分布） OSA 10是自由空间光学装置，其包括准直器组件15，衍射光栅20和反射镜22.发射尾纤通过准直器组件15将信号发射到自由空间中，并将其输入到衍射光栅20上 ，其在空间上分离或扩展准直的输入光，并将分散的光反射到反射镜22上.λ/ 4板26设置在反射镜22和衍射光栅20之间。反射镜将分离的光反射回λ/ 4板26连接到衍射光栅20，其通过准直透镜18反射光。透镜18将光（λ1-λN）的光谱带聚焦在空间中的不同焦点处。 光谱带11中的一个聚焦在接收引线28上，接收引线28然后传播到光电检测器30.枢转机构34围绕枢转点36枢转衍射光栅20或反射镜22，以顺序地或选择性地将每个光谱带11聚焦到 接收尾纤28.位置传感器42检测衍射光栅24或反射镜的位移。

公开(公告)号：US20040218172A1

公开(公告)日：2004-11-04

申请号：US10764113

申请日：2004-01-24

Inventor： Richard A. DeVerse ,  Frank B. Geshwind ,  Ronald R. Coifman ,  William G. Fateley ,  Andreas C. Coppi

IPC: G01J003/00 ,  G01N021/86

CPC classification number: G01N21/255 ,  G01J3/021 ,  G01J3/2823

Abstract: An adaptive digitally tuned light source is disclosed, in the form of a de-dispersive imaging spectrograph in both the visible and near infrared spectral regions. The devices are capable of illuminating a sample with appropriate energy-weighted spectral bands or spatio-spectral bands that relate only to the constituents of interest. The energy from each of the spectral resolution elements can be digitally modulated to provide a tuned weighted spectral output. A tuned light source device based on the present disclosure can be adapted for use in a conventional imaging microscope system to enable direct measure of spatio-spectral features of interest.

Abstract translation: 以可见光和近红外光谱区域中的去分散成像光谱仪的形式公开了一种自适应数字调谐光源。 这些装置能够用仅与感兴趣的成分相关联的适当的能量加权光谱带或空间光谱带照射样品。 来自每个光谱分辨率元素的能量可以被数字调制以提供调谐的加权光谱输出。 基于本公开的调谐光源装置可以适用于常规成像显微镜系统，以便直接测量感兴趣的空间光谱特征。

公开(公告)号：US20040201835A1

公开(公告)日：2004-10-14

申请号：US10492660

申请日：2004-04-10

Inventor： John Coates ,  Kenneth Mateer ,  Ronald Mellick

IPC: G01J003/00 ,  G01N021/17

CPC classification number: G01N21/53 ,  G01N21/0303 ,  G01N21/31 ,  G01N21/33 ,  G01N21/359 ,  G01N21/552 ,  G01N21/64 ,  G01N21/8507 ,  G01N33/2888 ,  G01N2021/3129 ,  G01N2021/3155

Abstract: A series of optical spectral sensors is based on a combination of solid-state sources (illumination) and detectors housed within an integrated package that includes the interfacing optics and acquisition and processing electronics. The focus is on low cost and the fabrication of the sensor is based on techniques that favor mass production. Materials and components are selected to support low-cost, high volume manufacturing of the sensors. Spectral selectivity is provided by the solid-state source(s) thereby eliminating the need for expensive spectral selection components. The spectral response covers the range from the visible (400 nm) to the mid-infrared (25,000 nm/25.0 nullm), as defined by the avalability of suitable low-cost solid-state source devices. A refractive optical system is employed, primarily in an internal reflection mode, allowing a selection of sample handling tools, including, but not restricted to internal reflectance and transmittance. A secondary channel allowing for light scattering or fluorescence methods is an option. The targeted applications of the sensing devices are for lubricants and functional fluids in the heavy equipment, automotive and transportation industries. A source reference channel is included to provide measurement stability and temperature compensation.

Abstract translation: 一系列光谱传感器基于固体源（照明）和包含接口光学器件和采集和处理电子器件的集成封装内的检测器的组合。 重点是低成本，并且传感器的制造基于有利于批量生产的技术。 选择材料和部件以支持传感器的低成本，高容量制造。 光谱选择性由固态光源提供，从而消除对昂贵光谱选择部件的需要。 光谱响应涵盖从可见（400nm）到中红外（25,000nm / 25.0mum）的范围，如合适的低成本固态光源装置的可用性所定义的。 采用折射光学系统，主要在内部反射模式中，允许选择样品处理工具，包括但不限于内部反射率和透射率。 允许光散射或荧光方法的次要通道是一种选择。 传感装置的目标应用是重型设备，汽车和运输行业中的润滑剂和功能流体。 包括源参考通道以提供测量稳定性和温度补偿。

公开(公告)号：US20040130713A1

公开(公告)日：2004-07-08

申请号：US10275954

申请日：2002-12-17

Inventor： Eon O'Mongain

IPC: G01J003/28 ,  G01J003/00

CPC classification number: G01N33/18 ,  G01N21/31 ,  G01N33/1826 ,  G01N2021/1793

Abstract: A spectrographic analysis method which is photometric and non-contact for determining the presence of a chosen constituent in water is provided based on the fact that the inverse of reflectance is a measure of absorption. A full spectrum measurement of the reflectance of the water is made and an absorption spectrum calculated which is then fitted to a clear spectrum in a wavelength range where the water absorption dominates so as to have scaling and offset. Then the clear water spectrum is subtracted and a matching is repeated for the spectrum of the desired constituent and thus a measure of the concentration of the constituent in the water is obtained.

Abstract translation: 基于反射率的倒数是吸收的量度的事实，提供用于测定水中所选组分的存在的光度和非接触性的光谱分析方法。 对水的反射率进行全谱测量，并计算吸收光谱，然后将其吸收在吸收占主导地位的波长范围内的清晰光谱上，以便具有缩放和偏移。 然后减去透明水谱，并对所需组分的光谱重复匹配，因此获得水中成分浓度的量度。

公开(公告)号：US20030184746A1

公开(公告)日：2003-10-02

申请号：US10107853

申请日：2002-03-26

Inventor： Howard A. Johnsen ,  James R. Ross ,  Sal R. Birtola

IPC: G01J003/00

CPC classification number: G01J5/041 ,  G01J3/02 ,  G01J3/0243 ,  G01J5/06 ,  G01J5/061 ,  G01N21/15 ,  G01N2021/151

Abstract: A shield is disclosed that is particularly useful for protecting exposed optical elements at the end of optical probes used in the analysis of hazardous emissions in and around an industrial environment from the contaminating effects of those emissions. The instant invention provides a hood or cowl in the shape of a right circular cylinder that can be fitted over the end of such optical probes. The hood provides a clear apertures through which the probe can perform unobstructed analysis. The probe optical elements are protected form the external environment by passing a dry gas through the interior of the hood and out through the hood aperture in sufficient quantity and velocity to prevent any significant mixing between the internal and external environments. Additionally, the hood is provided with a cooling jacket to lessen the potential for damaging the probe due to temperature excursions.

Abstract translation: 公开了一种屏蔽，其特别适用于在用于分析工业环境中及其周围的有害排放物的光学探针的末端，以防止这些排放物的污染影响，来保护暴露的光学元件。 本发明提供了可以安装在这种光学探头的端部上的右圆柱形的罩或罩。 护罩提供了一个清晰的孔，探针可以通过该孔进行无阻碍的分析。 探针光学元件通过使干燥气体通过发动机罩的内部并以足够的数量和速度穿过发动机罩孔而被外部环境保护，以防止内部和外部环境之间的任何显着的混合。 此外，罩设有冷却套，以减少由于温度偏移而导致探针损坏的可能性。

公开(公告)号：US20030160954A1

公开(公告)日：2003-08-28

申请号：US10370910

申请日：2003-02-20

Inventor： Yongdong Wang ,  Bernhard H. Radziuk ,  David H. Tracy

IPC: G01J003/00

CPC classification number: G01J3/28 ,  G01J3/42

Abstract: In a spectroscopic process a sample for producing a test spectral line or spectrum of at least one component contained in the sample is stimulated and the transmitted and/or emitted electromagnetic rays are used to create the test spectral line or spectrum. In order to improve such a spectroscopic process to such an extent that variations of certain parameters, which alter the shape and/or occurrence of a spectral line, are compensated, a comparison spectral line or spectrum of a known comparison material is produced under substantially the same parameters as the sample. The comparison spectral line or spectrum is compared with an ideal comparison spectral line or spectrum in order to calculate a transfer function, and the transfer function is applied to the test spectral line or spectrum in order to calculate a corrected test spectral line or spectrum.

Abstract translation: 在分光过程中，刺激用于产生测试谱线或样品中包含的至少一种组分的光谱的样品，并且使用透射和/或发射的电磁波来产生测试光谱线或光谱。 为了改进这种光谱过程，使得补偿了改变光谱线的形状和/或出现的某些参数的变化，已知比较材料的比较光谱线或光谱基本上在 与样品相同的参数。 将比较谱线或频谱与理想的比较谱线或频谱进行比较，以计算传递函数，并将传递函数应用于测试谱线或频谱，以便计算校正的测试谱线或频谱。

公开(公告)号：US20030081204A1

公开(公告)日：2003-05-01

申请号：US10226592

申请日：2002-08-23

Inventor： Paul J. Cronin ,  Peter J. Miller

IPC: G01J003/42 ,  G01J003/00 ,  G01N021/64

CPC classification number: G01N21/645 ,  G01N21/31 ,  G01N2021/6421

Abstract: A method is described that includes measuring, at each of a set of W wavelength bins, a spectral response of at least one region of a sample stained with multiple stains, and determining the concentration of at least one of the stains in the region of the sample based in part on the spectral responses, the wavelength bins being chosen so that a matrix of elements that represent the responses of the stains at the wavelength bin has an inverse for which a mathematical stability is maximum relative to the inverses of other matrices of elements that represent the responses of the stains for other possible sets of wavelength bins.

Abstract translation: 描述了一种方法，其包括在一组W波长区段中的每一个处测量被多个污渍染色的样品的至少一个区域的光谱响应，以及确定在多个污渍区域中的至少一个污渍的浓度 样品部分地基于光谱响应，选择波长区，使得表示波长箱上的污点的响应的元素矩阵具有相对于元素的其它矩阵的逆的数学稳定性最大的逆矩阵 其代表着对于其它可能的波长箱组的污渍的响应。