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    Separating agent for solid-phase extraction
    42.
    发明专利
    Separating agent for solid-phase extraction 有权
    固相萃取分离剂

    公开(公告)号：JP2006068597A

    公开(公告)日：2006-03-16

    申请号：JP2004252612

    申请日：2004-08-31

    Applicant: Shinwa Kako Kk ,  信和化工株式会社

    Inventor： WADA HIROO ,  FUKUZAWA KOSUKE

    IPC: B01J20/285 ,  B01D15/08 ,  B01J20/26 ,  B01J20/281 ,  G01N30/14 ,  G01N30/88

    CPC classification number: G01N1/405 ,  B01J20/26 ,  B01J20/286 ,  B01J20/3242 ,  B01J20/3268 ,  B01J20/3289 ,  B01J20/3293 ,  B01J2220/58 ,  B01J2220/62

    Abstract: PROBLEM TO BE SOLVED: To obtain a separating agent for solid-phase extraction which combines hydrophilicity and hydrophobicity, has a sharp distribution of particle sizes and allows low-cost manufacture with a high yield, and to provide its applications.
    SOLUTION: This separating agent consists of a core particle of a substrate, a hydrophilic polymer layer formed on the surface of the particle and a hydrophobic polymer layer formed on the surface of the hydrophilic polymer layer. A column or cartridge charged with the separating agent and a method for concentrating a target substance, a method for removing impurities, a method for solid-phase extraction of samples related to the environment, medicines or living bodies and a method for pretreating samples containing protein ingredients, with all the methods using the separating agent, are also provided.
    COPYRIGHT: (C)2006,JPO&NCIPI

    Abstract translation: 待解决的问题：为了获得组合亲水性和疏水性的固相萃取分离剂，具有大的颗粒尺寸分布，并允许低成本制造，并且提供其应用。 解决方案：该分离剂由基材的核心颗粒，形成在颗粒表面上的亲水性聚合物层和形成在亲水性聚合物层的表面上的疏水聚合物层组成。 装有分离剂的柱或筒，浓缩目标物质的方法，除去杂质的方法，固相萃取与环境相关的样品的方法，药物或活体的方法以及预处理含有蛋白质的样品的方法 还提供了使用分离剂的所有方法的成分。 版权所有（C）2006，JPO＆NCIPI

    Variant xylitol dehydrogenase enzyme, microorganism producing the same and method for converting xylitol to xylulose using the enzyme or microorganism
    43.
    发明专利
    Variant xylitol dehydrogenase enzyme, microorganism producing the same and method for converting xylitol to xylulose using the enzyme or microorganism 有权
    变性XYLITOL DEHYDROGENASE ENZYME，其生产的微生物和使用酶或微生物将XYLITOL转化为XYLULOSE的方法

    公开(公告)号：JP2006006213A

    公开(公告)日：2006-01-12

    申请号：JP2004188417

    申请日：2004-06-25

    Applicant: Kyoto Univ ,  Shinwa Kako Kk ,  信和化工株式会社 ,  国立大学法人京都大学

    Inventor： MAKINO KEISUKE ,  KOTAKI TSUTOMU ,  WATANABE SEIYA ,  WADA HIROO

    IPC: C12N15/09 ,  C12N1/19 ,  C12N1/21 ,  C12N9/02 ,  C12P7/06 ,  C12P19/02

    CPC classification number: Y02E50/17

    Abstract: PROBLEM TO BE SOLVED: To provide a variant xylytol dehydrogenase (XDH) having improved conversion efficiency of xylitol to xylulose by improving the coenzyme demand of wild XDH to nicotinamide adenine dinucleotide phosphate (NADP) type and improving the heat-resistance. SOLUTION: The coenzyme demand of a wild XDH is changed to NADP demand by substituting the 207th aspartic acid of the amino acid sequence to alanine, 208th isoleucine to arginine, 209th phenylalanine to threonine or tyrosine and 211th asparagine to arginine and the heat-resistance is improved by substituting the 96th serine to cysteine, the 99th serine to cysteine and 102nd tyrosine to cysteine and introducing a structure-stabilized zinc bonding site. COPYRIGHT: (C)2006,JPO&NCIPI

    Abstract translation: 待解决的问题：通过改善野生XDH对烟酰胺腺嘌呤二核苷酸磷酸（NADP）类型的辅酶需求并提高耐热性，提供木糖醇转化效率提高的木糖醇变体木糖醇脱氢酶（XDH）。 解决方案：通过将氨基酸序列的第207位天冬氨酸替换为丙氨酸，将第208位异亮氨酸替换为精氨酸，将第209位苯丙氨酸替换为苏氨酸或酪氨酸，将第211位天冬酰胺替换为精氨酸，将热量替换为NADP需求 将第96丝氨酸置换为半胱氨酸，将第99丝氨酸置换为半胱氨酸，将第102位酪氨酸置换为半胱氨酸并引入结构稳定的锌结合位点，从而改善了抗性。 版权所有（C）2006，JPO＆NCIPI

    Biological substance detector, novel biological substance structure used therefor, its manufacture, and its use
    44.
    发明专利
    Biological substance detector, novel biological substance structure used therefor, its manufacture, and its use 审中-公开

    公开(公告)号：JP2004177236A

    公开(公告)日：2004-06-24

    申请号：JP2002343040

    申请日：2002-11-26

    Applicant: Shinwa Kako Kk ,  信和化工株式会社

    Inventor： WADA HIROO ,  KANEHARA HIDEYUKI

    IPC: G01N33/53 ,  C12M1/00 ,  C12N15/09 ,  C12Q1/68 ,  G01N33/566 ,  G01N37/00

    Abstract: PROBLEM TO BE SOLVED: To provide a manufacturing method for a biological substance chip capable of detecting and determining quantitatively a target biological substance using the biological substance chip without modifying the target biological substance by a labeled compound, and a detection and quantitative determination method for the target biological substance. SOLUTION: The biological substance chip coupled preliminarily with the detected biological substance by hybridization is manufactured for a probe biological substance immobilized on a substrate, the target biological substance is hybridized to the probe biological substance on the biological substance chip, the detected biological substance is eliminated therefrom, and a variation of a signal from the detected biological substance is measured before and after the hybridization of the target biological substance to conduct the detection and the quantitative determination. The target biological substance is detected and determined quantitatively without labeling the target biological substance. COPYRIGHT: (C)2004,JPO

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