公开(公告)号：WO2014186697A3

公开(公告)日：2015-03-05

申请号：PCT/US2014038374

申请日：2014-05-16

Applicant: ADVANCED LIQUID LOGIC INC

Inventor： PUNNAMARAJU SRIKOUNDINYA ,  SANDAHL MELISSA A ,  HARRINGTON JOHN J ,  ECKHARDT ALLEN E ,  HUA ZHISHAN ,  POLLACK MICHAEL G

IPC: C12M1/42

CPC classification number: C12N15/87 ,  C12M33/00 ,  C12M35/02 ,  C12N13/00

Abstract: The invention provides a droplet actuator designed for performing electroporation on cells in droplets. The invention also provides method and systems for performing electroporation on cells in droplets on a droplet actuator.

Abstract translation: 本发明提供了一种设计用于在液滴上对细胞进行电穿孔的液滴致动器。 本发明还提供了用于在液滴致动器上的液滴中对细胞进行电穿孔的方法和系统。

公开(公告)号：WO2015010127A8

公开(公告)日：2015-01-22

申请号：PCT/US2014/047449

申请日：2014-07-21

Applicant: ADVANCED LIQUID LOGIC, INC.

Inventor： SMITH, Gregory ,  LUCK, Randall, L.

IPC: B05B15/00 ,  B05B1/24 ,  G01K13/00 ,  G01K7/00 ,  G01R19/155 ,  G01R27/08 ,  B05B12/00

Abstract: The present invention provides methods for on-actuator temperature measurement and temperature control, including where one or more of the temperature sensors are combined with one or more heaters that are formed of wiring traces and/or providing heaters designed for one-to- one correspondence to the temperature sensors to form temperature sensor-heater pairs. The present invention also provides methods for on-actuator temperature measurement and temperature control in which the temperature sensors comprise a connection comprising a plurality of terminals by which an amount of current can be applied and then a voltage measured, wherein the voltage that is measured across the temperature sensors can be accurately correlated to a temperature.

公开(公告)号：WO2013040562A3

公开(公告)日：2013-06-27

申请号：PCT/US2012055769

申请日：2012-09-17

Applicant: ADVANCED LIQUID LOGIC INC ,  YI UICHONG ,  SRINIVASAN VIJAY ,  WHITE ANDREW

Inventor： YI UICHONG ,  SRINIVASAN VIJAY ,  WHITE ANDREW

IPC: G01N35/10 ,  G01N35/08

CPC classification number: B01L3/0241 ,  B01L3/502715 ,  B01L3/502792 ,  B01L2200/0647 ,  B01L2200/0684 ,  B01L2200/0689 ,  B01L2200/143 ,  B01L2300/024 ,  B01L2300/025 ,  B01L2300/18 ,  B01L2400/0406 ,  B01L2400/043 ,  B01L2400/0457 ,  G01N35/10

Abstract: The present invention relates to apparatuses for delivering one or more volumes of fluids to one or more microfluidics devices. The invention further relates to methods for delivering one or more volumes of fluids to one or more microfluidics devices. Additionally, the invention relates to systems that include apparatuses for delivering one or more volumes of fluids to one or more microfluidics devices.

Abstract translation: 本发明涉及用于将一种或多种体积的流体输送到一个或多个微流体装置的设备。 本发明进一步涉及用于将一种或多种体积的流体递送至一个或多个微流体装置的方法。 另外，本发明涉及包括用于将一种或多种体积的流体输送到一个或多个微流体装置的装置的系统。

公开(公告)号：WO2013090889A1

公开(公告)日：2013-06-20

申请号：PCT/US2012/070055

申请日：2012-12-17

Applicant: ADVANCED LIQUID LOGIC INC ,  ECKHARDT, Allen

Inventor： ECKHARDT, Allen

IPC: G01N33/50 ,  G01N33/53 ,  G01N35/08 ,  G01N1/10

CPC classification number: B01L3/502715 ,  B01L3/502792 ,  B01L2200/027 ,  B01L2200/0668 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2400/0427 ,  G01N1/4077 ,  G01N33/54366 ,  G01N35/10 ,  G01N2035/1053

Abstract: The present invention relates to methods of sample preparation on a droplet actuator. The invention further relates to methods of plasma sample preparation on a droplet actuator by immunocapture. The invention also relates to methods of preparing plasma that contains HIV from whole blood without the need for filtration or centrifugation. Additionally, the invention relates to methods of sample preparation that use hydrophobic chromatography for purifying or removing unwanted materials from the sample. The invention further relates to a droplet actuator and methods of loading solutions of large volume into a droplet actuator under controlled conditions. The invention also relates to methods of processing cells on a droplet actuator.

Abstract translation: 本发明涉及液滴致动器上的样品制备方法。 本发明还涉及通过免疫捕获在液滴致动器上制备血浆样品的方法。 本发明还涉及制备从全血中含有HIV而不需要过滤或离心的血浆的方法。 另外，本发明涉及使用疏水色谱法从样品中净化或去除不想要的材料的样品制备方法。 本发明还涉及液滴致动器以及在受控条件下将大体积的溶液装载到液滴致动器中的方法。 本发明还涉及在液滴致动器上处理细胞的方法。

公开(公告)号：WO2013078216A1

公开(公告)日：2013-05-30

申请号：PCT/US2012/066084

申请日：2012-11-20

Applicant: ADVANCED LIQUID LOGIC INC ,  GRAHAM, Carrie, A. ,  ECKHARDT, Allen, E.

Inventor： GRAHAM, Carrie, A. ,  ECKHARDT, Allen, E.

IPC: C12Q1/26 ,  G01N33/68 ,  G01N33/52

CPC classification number: C12Q1/32 ,  G01N2333/904

Abstract: Aspects of embodiments may include methods for automated enzymatic detection of glucose-6-phosphate dehydrogenase (G6PD) activity. Aspects of embodiments may include methods for enzymatic detection of G6PD activity in droplets in oil. Aspects of embodiments may include a system including a droplet actuator. Aspects of embodiments may include a treatment method.

Abstract translation: 实施方案的各方面可以包括葡萄糖-6-磷酸脱氢酶（G6PD）活性的自动酶检测方法。 实施方案的各方面可以包括用于在油中的液滴中酶检测G6PD活性的方法。 实施例的方面可以包括包括液滴致动器的系统。 实施方案的各方面可以包括治疗方法。

公开(公告)号：WO2013009927A3

公开(公告)日：2013-01-17

申请号：PCT/US2012/046333

申请日：2012-07-11

Applicant: ADVANCED LIQUID LOGIC, INC. ,  WINGER, Theodore

Inventor： WINGER, Theodore

IPC: G01N15/02 ,  G01N27/02 ,  G01N33/573 ,  G01N33/53

Abstract: Droplet actuators and techniques for droplet-based assays are provided. A method of conducting an assay comprises: incubating a droplet in oil, the droplet comprising an umbelliferone substrate, a sample potentially comprising an enzyme which cleaves the umbelliferone substrate, and a zwitterionic surfactant; and detecting a signal emitted from the droplet.

公开(公告)号：WO2012151192A2

公开(公告)日：2012-11-08

申请号：PCT/US2012/035963

申请日：2012-05-01

Applicant: ADVANCED LIQUID LOGIC, INC. ,  BORT, Donovan ,  GRAHAM, Carrie ,  PAMULA, Vamsee ,  POLLACK, Michael ,  SISTA, Ramakrishna ,  SRINIVASAN, Vijay

Inventor： BORT, Donovan ,  GRAHAM, Carrie ,  PAMULA, Vamsee ,  POLLACK, Michael ,  SISTA, Ramakrishna ,  SRINIVASAN, Vijay

CPC classification number: G01N27/44791 ,  B01F1/0027 ,  B01F13/0071 ,  B01F13/0076 ,  B01F2001/0055 ,  B01F2215/0037 ,  B01L3/502761 ,  B01L3/502792 ,  B01L2200/0605 ,  B01L2200/0668 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/089 ,  B01L2400/0406 ,  B01L2400/0418 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0487 ,  B81B1/004

Abstract: A method of mixing a droplet, the method comprising providing a droplet on a surface, forming the droplet into a first "U" shape having a bottom region and two terminal ends, and simultaneously merging the terminal ends and splitting the droplet at the bottom region to form a second "U" shape which is substantially opposite the first "U" shape.

Abstract translation: 一种混合液滴的方法，所述方法包括在表面上提供液滴，将所述液滴形成为具有底部区域和两个末端的第一“U”形状，并同时合并末端并在底部区域分离液滴 以形成与第一“U”形大致相反的第二“U”形。

公开(公告)号：WO2012068055A3

公开(公告)日：2012-08-23

申请号：PCT/US2011060714

申请日：2011-11-15

Applicant: ADVANCED LIQUID LOGIC INC ,  POLLACK MICHAEL ,  STURMER RYAN ,  PAIK PHILIP ,  PAMULA VAMSEE ,  SRINIVASAN VIJAY

Inventor： POLLACK MICHAEL ,  STURMER RYAN ,  PAIK PHILIP ,  PAMULA VAMSEE ,  SRINIVASAN VIJAY

IPC: G01R27/26

CPC classification number: G01N27/221 ,  B01L3/502784 ,  B01L3/502792 ,  B01L2200/14 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/089 ,  B01L2400/0427 ,  B41J2/04541 ,  B41J2/04555 ,  B41J2/0458 ,  G01K7/34

Abstract: The embodiments described herein provide methods of measuring capacitance, detecting a droplet at a position, determining a thickness of an oil film and determining temperature in a droplet actuator. Specifically, the capacitance detection method may be used as a real-time verification tool in order to detect the absence, presence, and/or partial presence of a droplet at an electrode, analyze droplet properties, measure droplet size or volume, optimize the speed of droplet operation and detect air bubbles.

Abstract translation: 本文所述的实施例提供了测量电容，检测位置处的液滴，确定油膜的厚度和确定液滴致动器中的温度的方法。 具体地，可以将电容检测方法用作实时验证工具，以便检测电极处的液滴的不存在，存在和/或部分存在，分析液滴属性，测量液滴尺寸或体积，优化速度 的液滴操作并检测气泡。

公开(公告)号：WO2012012090A3

公开(公告)日：2012-03-29

申请号：PCT/US2011041761

申请日：2011-06-24

Applicant: ADVANCED LIQUID LOGIC INC ,  WANG TIH-HONG ,  BRACKETT GEORGE ,  CLEVENGER DAVID ,  BORT DONOVAN

Inventor： WANG TIH-HONG ,  BRACKETT GEORGE ,  CLEVENGER DAVID ,  BORT DONOVAN

IPC: B41J2/175 ,  B41F31/08

CPC classification number: B01L3/0241 ,  B01L3/502784 ,  B41J2/14024

Abstract: The invention provides droplet actuator assemblies and systems and methods of manufacturing the droplet actuator assemblies. In certain embodiments, two-piece enclosures are used to form a droplet actuator assembly that houses a droplet operations substrate. In certain other embodiments, one-piece enclosures are used to form a droplet actuator assembly that houses a droplet operations substrate. In the plastic injection molding process for forming substrates of the droplet actuator assemblies of the present invention may utilize insert molding (or overmolding) processes for forming a gasket in at least one substrate, thereby avoiding the need for providing and installing a separate gasket component. Further, the droplet actuator assemblies may include features that allow ultrasonic welding processes to be used for bonding substrates together. The manufacturing systems of the present invention for fabricating the droplet actuator assemblies may utilize continuous flow reel-to-reel manufacturing processes.

Abstract translation: 本发明提供了液滴致动器组件以及制造液滴致动器组件的系统和方法。 在某些实施例中，使用两件式外壳来形成容纳液滴操作基板的液滴致动器组件。 在某些其他实施例中，一体式外壳用于形成容纳液滴操作基板的液滴致动器组件。 在用于形成本发明的液滴致动器组件的基材的塑料注射成型工艺中，可利用嵌入成型（或二次成型）方法在至少一个基板上形成垫圈，从而避免提供和安装单独的衬垫部件。 此外，液滴致动器组件可以包括允许超声波焊接工艺用于将衬底粘合在一起的特征。 用于制造液滴致动器组件的本发明的制造系统可以利用连续流动卷对卷制造过程。

公开(公告)号：WO2011084703A3

公开(公告)日：2011-12-08

申请号：PCT/US2010061118

申请日：2010-12-17

Applicant: ADVANCED LIQUID LOGIC INC ,  ECKHARDT ALLEN E ,  GRAHAM CARRIE ,  SISTA RAMAKRISHNA ,  PAMULA VAMSEE K ,  WINGER THEODORE ,  WANG TONG ,  XIONG YALIN ,  WU NING ,  SAHA SANJAY ,  RAOLJI GAJENDRASINH BALVANTSINH ,  DAYAM RAVEENDRA

Inventor： ECKHARDT ALLEN E ,  GRAHAM CARRIE ,  SISTA RAMAKRISHNA ,  PAMULA VAMSEE K ,  WINGER THEODORE ,  WANG TONG ,  XIONG YALIN ,  WU NING ,  SAHA SANJAY ,  RAOLJI GAJENDRASINH BALVANTSINH ,  DAYAM RAVEENDRA

IPC: C12Q1/34 ,  C07D311/06 ,  C07D311/18 ,  C12M1/34 ,  C12Q1/25 ,  G01N33/52

CPC classification number: C12Q1/40 ,  B01L3/502784 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/18 ,  B01L2400/0427 ,  B01L2400/043 ,  C07D311/16 ,  C07D311/18 ,  C07D311/20 ,  C07D405/12 ,  C07D413/12 ,  C07H15/203 ,  C07H17/075 ,  C12Q1/25 ,  C12Q1/34 ,  C12Q1/44 ,  C12Q1/54 ,  C12Y302/01023 ,  G01N21/6452 ,  G01N33/5438 ,  G01N2333/916 ,  G01N2333/926 ,  G01N2333/938 ,  G01N2800/04

Abstract: The invention is directed to droplet actuator devices and assay methods for multiplexed newborn testing for metabolic disorders. The methods include, among other things, droplet-based enzymatic assays and immunoassays for testing for metabolic disorders. The invention includes methods and devices for conducting multiple assays for different metabolic disorders on a single droplet actuator, as well as multiple assays for the same metabolic disorder using samples from different subjects and/or multiple samples from the same subject on a single droplet actuator. In various embodiments, the invention includes methods for conducting enzymatic activity assays and/or immunoassays in a single fresh blood and/or plasma samples and dried blood and/or plasma samples.

Abstract translation: 本发明涉及用于代谢紊乱的多路复用新生儿测试的液滴致动器装置和测定方法。 除其他之外，所述方法包括基于液滴的酶测定和用于测试代谢紊乱的免疫测定。 本发明包括用于在单一液滴致动器上针对不同代谢病症进行多种测定的方法和装置，以及使用来自不同受试者的样品和/或来自单个液滴致动器上的来自相同受试者的多个样品的相同代谢病症的多种测定。 在各种实施方式中，本发明包括用于在单个新鲜血液和/或血浆样品以及干燥的血液和/或血浆样品中进行酶活性测定和/或免疫测定的方法。