公开(公告)号：US12005433B2

公开(公告)日：2024-06-11

申请号：US17327977

申请日：2021-05-24

Applicant: Douglas Scientific, LLC

Inventor： Richard J. Schoeneck ,  Paul H. Wagner

IPC: B01L3/02 ,  B08B3/12 ,  B08B9/023 ,  B08B9/032 ,  G01N35/10

CPC classification number: B01L3/0217 ,  B01L3/0234 ,  B08B3/12 ,  B08B9/023 ,  B08B9/0323 ,  G01N35/1004 ,  G01N35/1065 ,  B01L2200/0689 ,  B01L2200/141 ,  B01L2300/0829 ,  B01L2300/0861

Abstract: A pipettor includes a wash chamber between an upper plate, a lower plate, an upper seal, and a lower seal. The upper and lower seal retain each other and are located between the upper and lower plates. The pipettor includes a gasket located below the lower plate, a pipette tip retained by the gasket, and a piston with a tapered tip that passes through the plates, seals, and gasket into the pipette tip. The upper and lower seal isolate the piston from the wash chamber. The pipettor includes a channel defined by the piston, extending into the pipette tip, and an actuator that advances and retracts the piston. When the actuator retracts the piston such that the piston loses sealing contact with the lower seal and the wash chamber is supplied with wash fluid and pressurized, wash fluid passes through the groove in the upper seal and into the channel.

公开(公告)号：US11548005B2

公开(公告)日：2023-01-10

申请号：US16242884

申请日：2019-01-08

Applicant: DOUGLAS SCIENTIFIC, LLC

Inventor： Bruce Malcolm Peterson

IPC: B01L3/00 ,  B29C51/00 ,  B29C65/18 ,  B29C65/00 ,  B29L31/00

Abstract: A microplate (10) includes a carrier (12) having a plate (20) and an annular perimeter wall (30) to define a recess (34). An array of holes (26) extends through the plate (20). A tape piece (16), die cut from a flexible tape (60) includes an array of wells (54) each extending through and having an opening (56) extending into the well (54). The array of wells (54) has a number and locations corresponding to the array of holes (26). The openings (56) have sizes corresponding to the holes (26). An upper surface (50) of the tape piece (16) is abutted with and bonded to the bottom face (24) of the plate (20) with the openings (56) corresponding to the array of holes (26). The slideable receipt of an annular outer periphery (58) of the tape piece (16) insures that the array of wells (54) are aligned to correspond to the array of holes (26) as die cutting of tape piece (16) insures that the array of wells (54) are at consistent positions relative to the annular outer periphery (58).

公开(公告)号：US10456789B2

公开(公告)日：2019-10-29

申请号：US14888005

申请日：2014-05-01

Applicant: Douglas Scientific, LLC

Inventor： Chad Steven Smith ,  Jacob Hendrickx ,  Hans A. Mische

IPC: B08B9/032 ,  B01L3/02 ,  G01N35/10 ,  B01L99/00

Abstract: A method of washing an array of pipette tips (94) with residual biological material includes lowering the array of pipette tips (94) into a wash basin (20) that includes an array of flush ports (22). A vacuum is generated such that the residual biological material in the array of pipette tips (94) is evacuated through the array of flush ports (22). A wash cycle is performed that includes raising the array of pipette tips (94) from the wash basin (20), filling the wash basin (20) with a primary wash fluid, lowering the array of pipette tips (94) into the wash basin (20), miming a pipette tip cleansing cycle, and generating a vacuum such that the primary wash fluid in the array of pipette tips (94) and the wash basin (20) is evacuated through the array of flush ports (22).

公开(公告)号：US20190184401A1

公开(公告)日：2019-06-20

申请号：US16219737

申请日：2018-12-13

Applicant: Douglas Scientific, LLC

Inventor： Robert Hjelseth ,  Cory Hodgson ,  Andrew Bristow ,  Mark Baumgartner ,  David Sellnow ,  Cassie Keppel ,  John Steckelberg ,  Paul Newman

IPC: B01L3/00 ,  C12N15/10 ,  C12M1/00

CPC classification number: B01L3/5635 ,  B01L3/50 ,  B01L3/502 ,  B01L3/508 ,  B01L3/561 ,  B01L2200/026 ,  B01L2200/0631 ,  B01L2300/021 ,  B01L2300/042 ,  B01L2300/044 ,  B01L2300/0681 ,  B01L2300/0832 ,  B01L2400/0457 ,  C12M45/06 ,  C12N15/1017

Abstract: Embodiments of the invention provide a filter kit including filters for processing a biological sample. Some embodiments include a filter cap in a tube kit with a first tube containing a buffer solution and a second tube containing a lyophilized master mix. Some embodiments include a method of processing a sample using the kit including mixing a biological sample in a first tube with the buffer solution, positioning the filter cap in the first tube, positioning a second tube on the filter cap, flipping the first tube, the filter cap, and the second cap to filter the biological sample and buffer solution mixture with the filter cap as it flows from the first tube to the second tube. Some embodiments include structure enabling transfer of materials through inline flow between the tubes. Some further embodiments include integrated structure for sample pulverization with integrated buffer and lyophilized master mix.

公开(公告)号：US20180209877A1

公开(公告)日：2018-07-26

申请号：US15576212

申请日：2016-05-26

Applicant: DOUGLAS SCIENTIFIC, LLC

Inventor： JACOB P. HENDRICKX ,  CHAD S. SMITH ,  HANS A. MISCHE ,  MICHAEL BOTZET

IPC: G01N1/28 ,  G01N1/38 ,  G01N1/40 ,  B02C18/10 ,  B02C18/16 ,  B02C23/16 ,  B01D15/00

CPC classification number: G01N1/286 ,  B01D15/00 ,  B02C18/10 ,  B02C18/16 ,  B02C23/16 ,  B02C2023/165 ,  G01N1/38 ,  G01N1/4077 ,  G01N2001/2866 ,  G01N2001/4088

Abstract: A seed grinder includes a housing that is configured to contain seeds and a buffer solution, a grinding mechanism that is positionable in the housing to grind the seeds in the housing, and a dispensing mechanism attached to the housing and configured to dispense the ground seeds and the buffer solution from the housing into a sample holder.

公开(公告)号：US20180202932A1

公开(公告)日：2018-07-19

申请号：US15561901

申请日：2016-03-25

Applicant: DOUGLAS SCIENTIFIC, LLC

Inventor： HANS MISCHE ,  GRANT PEXSA ,  MARK BAUMGARTNER ,  CORY HODGSON ,  ANDREW BRISTOW ,  JOHN STECKELBERG ,  JARED PATTERSON

IPC: G01N21/64 ,  G01N21/25 ,  B01L7/00 ,  G01N33/50 ,  B01L3/00

CPC classification number: G01N21/6428 ,  B01L3/527 ,  B01L7/52 ,  B01L2300/0609 ,  B01L2300/0893 ,  G01N21/253 ,  G01N21/645 ,  G01N33/50 ,  G01N2021/6439 ,  G01N2021/6482 ,  G01N2201/024 ,  G01N2201/062 ,  G01N2201/0691

Abstract: A modular testing device includes a base unit and an expansion unit that communicates with the base unit. The expansion unit includes a housing, a receptacle in which a sample holder containing a biological sample and reagent mixture can be placed, and an optical assembly positioned in the housing. The optical assembly is configured to amplify and detect a signal from the biological sample and reagent mixture. Data that is collected in the optical assembly is communicated to the base unit.

公开(公告)号：US20160332155A1

公开(公告)日：2016-11-17

申请号：US15223737

申请日：2016-07-29

Applicant: Douglas Scientific, LLC

Inventor： Richard J. Schoeneck ,  Paul H. Wagner

IPC: B01L3/02 ,  B08B3/12 ,  B08B9/023 ,  G01N35/10 ,  B08B9/032

CPC classification number: B01L3/0217 ,  B01L3/0234 ,  B01L2200/0689 ,  B01L2200/141 ,  B01L2300/0829 ,  B01L2300/0861 ,  B08B3/12 ,  B08B9/023 ,  B08B9/0323 ,  G01N35/1004 ,  G01N35/1065

Abstract: A pipettor includes a wash chamber between an upper plate, a lower plate, an upper seal, and a lower seal. The upper and lower seal retain each other and are located between the upper and lower plates. The pipettor includes a gasket located below the lower plate, a pipette tip retained by the gasket, and a piston with a tapered tip that passes through the plates, seals, and gasket into the pipette tip. The upper and lower seal isolate the piston from the wash chamber. The pipettor includes a channel defined by the piston, extending into the pipette tip, and an actuator that advances and retracts the piston. When the actuator retracts the piston such that the piston loses sealing contact with the lower seal and the wash chamber is supplied with wash fluid and pressurized, wash fluid passes through the groove in the upper seal and into the channel.

公开(公告)号：US20160265040A1

公开(公告)日：2016-09-15

申请号：US15027797

申请日：2014-10-07

Applicant: DOUGLAS SCIENTIFIC, LLC

Inventor： Mark B. Baumgartner ,  Cory D. Hodgson ,  Andrew L. Bristow ,  Paul A. Syverson ,  Grant L. Pexsa ,  Christopher T. Davis ,  Rich Schoeneck ,  Duane Brown ,  Darren Cook

IPC: C12Q1/68 ,  B01L7/00 ,  B01L3/00 ,  G01N21/64 ,  B01L9/06

CPC classification number: C12Q1/6848 ,  B01L3/50851 ,  B01L7/00 ,  B01L9/06 ,  B01L2200/10 ,  B01L2200/141 ,  B01L2300/021 ,  B01L2300/024 ,  B01L2300/025 ,  B01L2300/041 ,  B01L2300/0654 ,  G01N21/0332 ,  G01N21/253 ,  G01N21/6428 ,  G01N21/645 ,  G01N2021/0325 ,  G01N2021/6441 ,  G01N2021/6482 ,  G01N2201/0221 ,  G01N2201/0231

Abstract: A portable testing device includes a housing with an integrated touchscreen display and a receptacle in which a sample holder containing a biological sample and reagent mixture can be placed. The portable testing device further includes an optical assembly positioned in the housing, an electronic assembly that is configured to receive data from the optical assembly and transmit it for display on the touchscreen display, and a power supply in the housing to power the portable testing device. The optical assembly includes an excitation filter that extends across the entire optical assembly and an emission filter that extends across the entire optical assembly.

Abstract translation: 便携式测试装置包括具有集成的触摸屏显示器的壳体和容纳有容纳生物样品和试剂混合物的样品架的容器。 便携式测试装置还包括定位在壳体中的光学组件，电子组件，其被配置为从光学组件接收数据并将其发送以在触摸屏显示器上显示，以及壳体中的电源以为便携式测试装置 。 光学组件包括延伸穿过整个光学组件的激发滤光器和延伸穿过整个光学组件的发射滤光器。

公开(公告)号：US20160016161A1

公开(公告)日：2016-01-21

申请号：US14776505

申请日：2014-03-14

Applicant: DOUGLAS SCIENTIFIC, LLC

Inventor： Richard J. Schoeneck ,  Paul H. Wagner

IPC: B01L3/02

CPC classification number: B01L3/0217 ,  B01L3/0234 ,  B01L2200/0689 ,  B01L2200/141 ,  B01L2300/0829 ,  B01L2300/0861 ,  B08B3/12 ,  B08B9/023 ,  B08B9/0323 ,  G01N35/1004 ,  G01N35/1065

Abstract: A pipettor includes a wash chamber between an upper plate, a lower plate, an upper seal, and a lower seal. The upper and lower seal retain each other and are located between the upper and lower plates. The pipettor includes a gasket located below the lower plate, a pipette tip retained by the gasket, and a piston with a tapered tip that passes through the plates, seals, and gasket into the pipette tip. The upper and lower seal isolate the piston from the wash chamber. The pipettor includes a channel defined by the piston, extending into the pipette tip, and an actuator that advances and retracts the piston. When the actuator retracts the piston such that the piston loses sealing contact with the lower seal and the wash chamber is supplied with wash fluid and pressurized, wash fluid passes through the groove in the upper seal and into the channel.

Abstract translation: 移液器包括在上板，下板，上密封和下密封之间的洗涤室。 上下密封彼此保持并位于上板和下板之间。 移液器包括位于下板下方的垫圈，由垫圈保持的移液管末端，以及具有锥形尖端的活塞，其通过板，密封件和垫圈进入移液管尖端。 上下密封将活塞与洗涤室隔离开。 移液器包括由活塞限定的通道，延伸到移液管末端，以及促进和缩回活塞的致动器。 当致动器缩回活塞，使得活塞与下部密封件密封接触，并且洗涤室被供应洗涤流体并加压，洗涤流体通过上部密封件中的凹槽并进入通道。

公开(公告)号：US20150322489A1

公开(公告)日：2015-11-12

申请号：US14762325

申请日：2014-01-23

Applicant: DOUGLAS SCIENTIFIC, LLC

Inventor： Hans A. Mische

IPC: C12Q1/68 ,  G01N1/40

CPC classification number: C12Q1/6806 ,  B01L3/0255 ,  C07H1/06 ,  C07H1/08 ,  C07H21/00 ,  G01N1/405

Abstract: An apparatus for isolating nucleic acids includes an elongated body. The elongated body includes a silica surface positioned and configured to bind the nucleic acids when the elongated body is dipped into a biological material sample.

Abstract translation: 用于分离核酸的装置包括细长体。 细长体包括二氧化硅表面，其被定位并构造成当细长体浸入生物材料样品中时结合核酸。