中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

92 records (took 0.029 seconds)

    Method and apparatus for fluorescence measurements
    43.
    发明公开
    Method and apparatus for fluorescence measurements 失效
    Verfahren und VorrichtungfürFluoreszenzmessungen。

    公开(公告)号：EP0421156A2

    公开(公告)日：1991-04-10

    申请号：EP90117473.0

    申请日：1990-09-11

    Applicant: Packard Instrument Company, Inc.

    Inventor： Osten, Donald E.

    IPC: G01N21/64

    CPC classification number: G01N21/645 ,  G01N2021/516 ,  G01N2021/6469

    Abstract: An improved method and apparatus is provided for performing high sensitivity fluorescence measurements. A liquid sample (12) contained within a cuvette (13) is exposed to exciting radiation in such a way that the radiation is introduced directly to the sample (12) and is restricted from reaching the container walls. The sample container (33) is in the form of a cylindrical cuvette provided with reflector means on the bottom so that any unabsorbed exciting radiation reaching the cuvette bottom is reflected back through the sample solution. Fluorescent radiation emitted by the sample (12) is collected by an ellipsoidal reflector (64) which substantially surrounds the sample container (33) and focuses radiation incident thereupon onto a small area from which it is efficiently sensed by a photodetector (66). The optical arrangement realizes significantly improved signal-to-noise and signal-to-background ratios.

    Abstract translation: 提供了一种用于执行高灵敏度荧光测量的改进的方法和装置。 容纳在比色皿（13）内的液体样品（12）暴露于激发的辐射，使得辐射直接引入样品（12）并被限制到达容器壁。 样品容器（33）是圆柱形比色皿的形式，在底部设置有反射器装置，使得到达比色皿底部的任何未吸收的激发辐射通过样品溶液被反射回来。 由样品（12）发射的荧光辐射由基本上围绕样品容器（33）的椭球反射器（64）收集，并将入射到其上的辐射聚焦到由光电检测器（66）有效感测的小区域上。 光学装置实现了显着改善的信噪比和信号对背景比。

    Apparatus and method for generating hydrogen and oxygen by electrolytic dissociation of water
    44.
    发明公开
    Apparatus and method for generating hydrogen and oxygen by electrolytic dissociation of water 失效
    装置和用于通过水的电解分解产生氢气和氧气的方法。

    公开(公告)号：EP0417647A1

    公开(公告)日：1991-03-20

    申请号：EP90117191.8

    申请日：1990-09-06

    Applicant: Packard Instrument Company, Inc.

    Inventor： Young, Stuart A. ,  Zweifel, Ronald A. ,  Caldwell, Daniel L.

    IPC: C25B15/00 ,  C25B1/10

    CPC classification number: C25B15/00 ,  C25B1/10 ,  Y02E60/366

    Abstract: Apparatus for generating hydrogen by the electrolytic dissociation of water comprises an electrolytic cell (C) having a cathode (13) and an anode (12) separated by an electrolyte (14), an electrical power supply connected to the cell for applying a voltage across the cathode and anode, a water reservoir (1),(2) connected to the cell for supplying water to the anode side of the electrolytic cell, a hydrogen-water separator (21) connected to the cell for receiving hydrogen and water from the cathode side of the cell and separating the hydrogen from the water, and a water return line (25) connecting the hydrogen-water separator to the water reservoir for returning water from the separator to the water reservoir whereby the water is recycled to the anode side of the cell. A float valve in the upper region of the hydrogen-­water separator closes the hydrogen outlet (30) in response to an increase in the water level in the separator to the level of the hydrogen outlet, so as to prevent water from entering the hydrogen outlet in the event of a malfunction. A pressure relief valve (210) in the hydrogen-water separator discharges hydrogen from the separator to the atmosphere in response to an increase in the gas pressure in the separator beyond a predetermined level. A sensor (3) in the water reservoir produces an electrical signal in response to a drop in the water level in the reservoir to a predetermined level, or in response to a predetermined change in the electrical conductivity of the water, and control circuitry responds to the electrical signal for interrupting the supply of electrical power to the cell.

    Abstract translation: 装置用于通过具有阴极（13）的电解槽（C）的水包含的电解分解产生氢，并通过在连接到电池供电的电解质（14），用于在施加电压分离的阳极（12） 连接到单元用于供应水到电解池的阳极侧上的阴极和阳极，一个贮水器（1），（2），连接到所述电池的氢 - 水分离器（21），用于从所述接收氢和水 所述细胞，并从水中分离的氢，和连接氢 - 水分离器将水贮存器，用于从分离器返回水到水贮存由此水被再循环到阳极侧的水返回线路（25）的阴极侧 细胞。 在氢 - 水分离器的上部区域中的浮阀关闭响应于在分离器到氢出口的水平的水位上升的氢气出口（30），以防止水进入所述氢气出口 在故障的情况下。 压力释放阀（210）中的氢 - 水分离器排出的氢从分离到大气中响应于在超过预定水平分离器中的气体压力增加。 电信号的在储水器中的传感器（3）产生响应于在储存到预定水平的水位的下降，或者响应于在水的导电率的预定变化，和控制电路来响应 该电信号用于中断给电池的电功率的供给。

    Liquid scintillation measurement system with solid crystal guard shield
    46.
    发明公开
    Liquid scintillation measurement system with solid crystal guard shield 失效
    Flüssigkeits-Szintillationsmessgerätmit Festkristallabschirmung。

    公开(公告)号：EP0253484A1

    公开(公告)日：1988-01-20

    申请号：EP87304873.0

    申请日：1987-06-02

    Applicant: Packard Instrument Company, Inc.

    Inventor： Valenta, Robert J.

    IPC: G01T1/204 ,  G01T1/202

    CPC classification number: G01T1/202 ,  G01T1/204

    Abstract: A liquid scintillation measurement system is described which provides increased accuracy of measure­ment for low-level liquid scintillation-counting utilizes the combination of a "burst-counting" technique aimed at detecting photon pulse bursts which characteristically follow coincident pulses produced by scintillation resulting from background radioactivity with an active guard shield formed around the liquid scintillation sample in order to enhance the pulse bursts normally following background events and hence more accurately identify scintillations produced by actual sample radioactivity. The active guard shield comprises a high scintillation capacity solid crystal material (104) which is easily excited by external radioactivity and is formed as an integrally structured unit with the optical event detection means (12,14) of the measurement system. The shield may also be provided in the form of a liquid scintillation vial (120) (fig. 3) composed of a high scintillation solid crystal material (124) for containing the liquid sample, with the vial being coated on its internal surface with an electron impermissive layer (126) to prevent the sample radiation from exciting the solid scintillator material. Alternatively, the active guard shielding effect may be obtained by using a liquid scintillation cocktail (Fig. 4) in which the liquid sample (134) is mixed with coated beads (136) of the solid scintillation material (138).

    Abstract translation: 描述了液体闪烁测量系统，其提供了用于低水平液体闪烁计数的增加的测量精度，其利用“突发计数”技术的组合，其旨在检测光子脉冲脉冲串，其特征在于遵循由背景放射性产生的闪烁产生的重合脉冲 具有形成在液体闪烁样品周围的主动防护屏蔽，以便通过背景事件增强脉冲突发，从而更精确地识别由实际样品放射性产生的闪烁。 主动防护罩包括高闪烁能力的固体晶体材料（104），其易于被外部放射性激发，并且与测量系统的光学事件检测装置（12,14）形成为整体结构的单元。 屏蔽还可以由用于容纳液体样品的高闪烁固体晶体材料（124）组成的液体闪烁管（120）（图3）的形式提供，小瓶在其内表面上涂覆有 电子不发射层（126），以防止样品辐射激发固体闪烁体材料。 或者，可以通过使用其中液体样品（134）与固体闪烁材料（138）的涂覆珠粒（136）混合的液体闪烁混合物（图4）来获得主动防护屏蔽效果。

    Vented vial holder for reducing static electricity
    47.
    发明公开
    Vented vial holder for reducing static electricity 失效
    VentilierterGlasflaschenbehälterzur Minderung statischerElektrizität。

    公开(公告)号：EP0213690A2

    公开(公告)日：1987-03-11

    申请号：EP86303854.3

    申请日：1986-05-21

    Applicant: Packard Instrument Company, Inc.

    Inventor： O'Day, Benedict Raymond ,  De Filippis, John Stanley

    IPC: G01N1/00 ,  G01N1/28 ,  G01N23/10 ,  G01T1/20

    CPC classification number: G01T1/204 ,  G01T1/2047 ,  G01T7/02

    Abstract: A holder (10) for use with scintillation counting for securing a small sample vial (50) in a vial trans­port carrier designed for larger vials is disclosed. The holder includes specific features for allowing elimination of static electricity charges on the sample vial by a static eliminator. The holder includes openings (32,34,36) allowing the flow of ions to contact the sample vial (50) for the purpose of eliminating static charges on the surface of the vial.

    Abstract translation: 公开了一种用于闪烁计数的保持器（10），用于将小样品瓶（50）固定在为较大瓶子设计的小瓶输送载体中。 支架包括具有特殊功能，可通过静电消除器消除样品瓶上的静电电荷。 保持器包括允许离子流接触样品瓶（50）的开口（32,34,36），以消除小瓶表面上的静电荷。

    METHOD AND COMPOSITIONS FOR DETECTING LUCIFERASE IN BIOLOGICAL SAMPLES
    48.
    发明授权
    METHOD AND COMPOSITIONS FOR DETECTING LUCIFERASE IN BIOLOGICAL SAMPLES 有权
    方法和组合物荧光素酶检测生物样品

    公开(公告)号：EP1060263B1

    公开(公告)日：2007-05-23

    申请号：EP99903144.6

    申请日：1999-01-18

    Applicant: Packard Instrument Company, Inc.

    Inventor： ROELANT, Christiaan

    IPC: C12Q1/66 ,  C12N9/99 ,  G01N21/76

    CPC classification number: C12Q1/66

    Abstract: The presence of renilla luciferase alone or both renilla luciferase and firefly luciferase is detected by adding reagent mixture(s) to a biological sample and producing glow luminescence having a duration of at least an hour. In producing luminescence from renilla luciferase alone, a reagent is added comprising coelenterazine, and dithiothreitol (DTT) and EDTA, or functional equivalents of DTT and EDTA. Luminescence from samples containing both firefly luciferase and renilla luciferase is produced by first adding a reagent comprising firefly luciferin, ATP, co-factors necessary for firefly luciferase activity (e.g., Ca and Mg ), dithiothreitol (DTT) or functional equivalents thereof, and AMP. Following measurement of the firefly luciferase, coelenterazine and EDTA, or functional equivalents of EDTA, are added, and the luminescence produced by renilla luciferase is measured.

Patent Agency Ranking