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    43.
    发明专利
    未知

    公开(公告)号：DE69535181T2

    公开(公告)日：2007-08-23

    申请号：DE69535181

    申请日：1995-12-21

    Applicant: SEMBIOSYS GENETICS INC

    Inventor： MOLONEY MAURICE

    IPC: C12N15/82 ,  A01H5/00 ,  C07K14/545 ,  C07K14/61 ,  C07K14/81 ,  C07K14/815 ,  C12N9/64 ,  C12N9/74 ,  C12N15/15 ,  C12N15/25 ,  C12N15/29 ,  C12N15/57 ,  C12N15/62

    Abstract: The present invention relates to the use of a class of genes called oil body protein genes that have unique features. The discovery of these features allowed the invention of methods for the production of recombinant proteins wherein a protein of interest can be easily separated from other host cell components. The invention is further exemplified by methods for exploitation of the unique characteristics of the oil body proteins and oil body genes for expression of polypeptides of interest in many organisms, particularly plant seeds. Said polypeptides may include but are not limited to: seed storage proteins, enzymes, bioactive peptides, antibodies and the like. The invention can also be modified to recover recombinant polypeptides fused to oleosins from non-plant host cells. Additionally the invention provides a method of using recombinant proteins associated with seed oil bodies released during seed germination for expression of polypeptides that afford protection to seedlings from pathogens. Finally, the persistent association of oil body proteins with the oil body can be further utilized to develop a biological means to create novel immobilized enzymes useful for bioconversion of substrates.

    Methods for the modulation of oleosin expression in plants
    45.
    发明专利
    Methods for the modulation of oleosin expression in plants 未知

    公开(公告)号：AU2005291805A1

    公开(公告)日：2006-04-13

    申请号：AU2005291805

    申请日：2005-10-06

    Applicant: SEMBIOSYS GENETICS INC ,  UTI LP

    Inventor： SILOTO RODRIGO M ,  MOLONEY MAURICE M

    IPC: C12N15/82 ,  A01H5/00 ,  C11B1/00 ,  C12N15/29

    Abstract: Methods to modulate oleosin expression levels in plants are provided. Specifically, methods for preparing seed derived products from seed, in which the composition of seed storage reserves, notably the seed lipid and protein contents, have been altered. In particular the present invention provides methods for preparing seed derived products from seed, in which the seed reserves have been altered by modulation of oleosin gene expression and more particularly the suppression of oleosin gene expression.

    METODOS PARA LA PRODUCCION DE INSULINA
    46.
    发明专利
    METODOS PARA LA PRODUCCION DE INSULINA 未知

    公开(公告)号：AR044803A1

    公开(公告)日：2005-10-05

    申请号：ARP040102114

    申请日：2004-06-17

    Applicant: SEMBIOSYS GENETICS INC

    Inventor： MOLONEY MAURICE ,  BOOTHE JOSEPH ,  KEON RICHARD ,  NYKIFORUK CORY ,  VAN ROOIJEN GIJS

    IPC: A01H1/00 ,  C07K14/62 ,  C12N15/82 ,  C12N15/17 ,  C12N15/90 ,  A01H5/00 ,  A01H5/10

    Abstract: Reivindicación 1: Un método para la expresión de insulina en semillas vegetales, caracterizado porque comprende: a) la provisión de una construcción quimérica de ácido nucleico que comprende, como componentes operativamente ligados en la dirección de transcripción 5' a 3': i) una secuencia de ácido nucleico con la capacidad de controlar la expresión en células de semillas vegetales; y ii) una secuencia de ácido nucleico que codifica para un polipéptido insulínico; b) la introducción de la construcción quimérica de ácido nucleico dentro de una célula vegetal; y c) la multiplicación de la célula vegetal dentro de una planta madura con la capacidad de producir simiente, en donde la simiente expresa insulina. Reivindicación 28: Una planta con la capacidad de producir semillas que comprenden una secuencia quimérica de ácido nucleico caracterizado porque comprende, en la dirección de transcripción 5' a 3': a) una primera secuencia de ácido nucleico con la capacidad de controlar la expresión en una célula de semilla vegetal a la que está operativamente ligada; b) una segura secuencia de ácido nucleico codificadora de un polipéptido insulínico, en donde la semilla contiene insulina. Reivindicación 31: Una semilla vegetal que comprende una secuencia quimérica de ácido nucleico caracterizado porque comprende, en la dirección de transcripción 5' a 3': a) una primera secuencia de ácido nucleico con la capacidad de controlar la expresión en una célula de semilla vegetal a la que está operativamente ligada; b) una segunda secuencia de ácido nucleico que codifica para un polipéptido insulínico. Reivindicación 33: Una secuencia de ácido nucleico codificadora de insulina, ligada a una secuencia de ácido nucleico caracterizado porque comprende un promotor con la capacidad de controlar la expresión en una célula de semilla vegetal.

    Method for recovery of recombinantly produced polypeptides involving the cleavage of fusion proteins
    48.
    发明专利
    Method for recovery of recombinantly produced polypeptides involving the cleavage of fusion proteins 未知

    公开(公告)号：NZ500736A

    公开(公告)日：2005-01-28

    申请号：NZ50073698

    申请日：1998-04-23

    Applicant: SEMBIOSYS GENETICS INC

    Inventor： MOLONEY MAURICE ,  ALCANTARA JOENEL ,  VAN ROOIJEN GIJS

    IPC: C12N15/09 ,  A23L1/305 ,  A61K38/27 ,  A61K38/55 ,  A61P3/02 ,  A61P43/00 ,  C07K14/61 ,  C07K14/815 ,  C07K19/00 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/50 ,  C12N15/62 ,  C12P21/02 ,  C12N15/15 ,  C12N15/57 ,  A61K38/58

    Abstract: A method for the preparation of a recombinant polypeptide comprising a) Introducing into a host cell an expression vector comprising: 1) A nucleic acid sequence capable of regulating transcription into a host cell, operatively linked to 2) A chimeric nucleic acid sequence encoding a fusion protein, the chimeric nucleic acid sequence comprising (a) A nucleic acid sequence encoding a pro-peptide derived from an autocatalytically maturing zymogen, linked in reading frame to (b) a nucleic acid sequence heterologous to the pro-peptide and encoding the recombinant polypeptide, wherein the heterologous nucleic acid sequence is located immediately downstream of the nucleic acid sequence encoding the pro-peptide; operatively linked to 3) A nucleic acid sequence encoding a termination region functional in said host cell b) Growing the host cell to produce said fusion protein; and c) Altering the environment of the fusion protein so that the pro-peptide is cleaved from the fusion protein to release the recombinant polypeptide, but excluding a human host cell in situ. Altering the environment can take place under in vitro or in vivo conditions. The fusion protein produced can be used to prepare pharmaceutical compositions or food compositions. The medicaments prepared are for delivering a therapeutic or nutritional protein to a human or animal.

    49.
    发明专利
    未知

    公开(公告)号：AT277180T

    公开(公告)日：2004-10-15

    申请号：AT92908088

    申请日：1992-04-15

    Applicant: SEMBIOSYS GENETICS INC

    Inventor： MOLONEY MAURICE M

    IPC: C12N15/09 ,  A01H5/00 ,  C07K20060101 ,  C07K14/415 ,  C07K14/61 ,  C07K14/81 ,  C07K19/00 ,  C12N20060101 ,  C12N5/10 ,  C12N9/64 ,  C12N15/29 ,  C12N15/62 ,  C12N15/82 ,  C12P21/02

    Abstract: Methods and compositions for use therein are described for expressing a polypeptide of interest in a seed cell as a fusion protein with an oil body protein. By this means, the fusion protein is targeted to the oil bodies of a seed cell. The oil body is easily separated from other cellular material following lysis of the seed cell, for example by using the partitioning/surface properties of the oil body. The fusion protein may be isolated for example by affinity chromatography using antibodies directed to the oil body protein. Where desired, the polypeptide of interest can be recovered by treatment of the fusion protein with for example a protease capable of recognizing a proteolytic recognition site in the oil body protein proximal to the N-terminus of the polypeptide of interest.

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