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公开(公告)号：KR1020180002949A
公开(公告)日：2018-01-09
申请号：KR1020160081877
申请日：2016-06-29
Applicant: 강릉원주대학교산학협력단
Inventor： 최석정
CPC classification number: C12N15/1013 , B01L3/5023 , C12Q1/68 , C12Q1/6806 , G01N33/56966 , G01N35/0098
Abstract: 본발명은시료공간(sample chamber), 용해공간(lysis chamber), 용리공간(elution chamber) 및상기시료공간, 용해공간, 용리공간을연결하는채널로구성된정지액체상랩온어칩을이용하여, 상기시료공간에세포를포함하는시료와세포포획입자(cell capture particle, CCP)를첨가하여세포-CCP 복합체를형성하는단계; 상기세포-CCP 복합체를용해공간으로이동시키고, 상기세포를용해시켜서핵산을상기세포로부터방출하는단계; 상기생성된핵산을핵산포획입자(nucleic acid capture particle, NCP) 로포획하여핵산-NCP 복합체를형성하는단계; 및상기핵산-NCP 복합체를용리공간으로이동시켜핵산을용리시키는단계; 를포함하는정지액체상랩온어칩을이용한핵산시료의제조방법에관한것이다.
Abstract translation: 本发明涉及一种使用由样品室，裂解室，洗脱室和连接样品空间的通道组成的固定液相片状包裹片来分析样品的方法和设备， 将含有细胞和细胞捕获颗粒（CCP）的样品添加到空间以形成细胞-CCP复合物; 将细胞-CCP复合物转移至溶解空间，并溶解细胞以从细胞释放核酸; 用核酸捕获颗粒（NCP）捕获得到的核酸以形成核酸-NCP复合物; 并将核酸-NCP复合物转移至洗脱空间以洗脱核酸; 本发明涉及一种使用固定液相包裹芯片生产核酸样品的方法。
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公开(公告)号：KR101811134B1
公开(公告)日：2017-12-22
申请号：KR1020160081866
申请日：2016-06-29
Applicant: 강릉원주대학교산학협력단
Inventor： 최석정
IPC: G01N33/569 , G01N35/08 , G01N33/543 , G01N33/58 , B01L3/00
CPC classification number: G01N33/56911 , B01L3/502753 , B01L2200/10 , B01L2300/0681 , B01L2300/0861 , G01N33/54326 , G01N33/582 , G01N35/08
Abstract: 본발명은배양공간, 세척공간, 검출공간및 상기배양공간, 세척공간, 검출공간을연결하는채널로이루어진정지액체상랩온어칩을이용하여, 상기배양공간에시료를넣고박테리아를배양하는단계; 상기배양공간에서포획입자-박테리아-표지입자복합체를형성하는단계; 상기포획입자-박테리아-표지입자복합체를상기배양공간의하부에서포획하는단계; 상기포획입자-박테리아-표지입자복합체를배양공간으로부터검출공간으로이동시키는단계; 및상기검출공간에서상기표지입자의신호를측정하여박테리아를검출하는단계;를포함하는것을특징으로하는정지액체상랩온어칩을이용한박테리아검출방법에관한것이다.
Abstract translation: 本发明的使用的培养空间，清洁的空间，检测空间，并停止由上实验室一个片内通道连接所述培养空间，清洁的空间，检测空间的液相，然后将样品放入孵化空间培养细菌; 在培养空间中形成捕获颗粒 - 细菌标记的颗粒复合物; 在培养空间的底部捕获捕获的颗粒 - 细菌标记的颗粒复合物; 将捕获的颗粒 - 细菌 - 标记的颗粒复合物从培养空间转移到检测空间; 并检测通过测量在检测空间标记颗粒的信号的细菌;涉及使用所述液体仍上实验室一个芯片包括一个细菌检测方法：一。
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公开(公告)号：KR1020160101775A
公开(公告)日：2016-08-26
申请号：KR1020150024104
申请日：2015-02-17
Applicant: 강릉원주대학교산학협력단
Inventor： 최석정
IPC: B01L3/00 , B01L9/00 , G01N27/30 , G01N27/327
CPC classification number: B01L3/5027 , B01L9/527 , B01L2200/10 , G01N27/3271 , G01N27/3277
Abstract: 본발명은입자(particle)를포함하는반응물(reactant)과분석물질(analyte)을포함하는시료(sample)의혼합용액이담겨지는시료공간; 상기시료공간의하부와연결설치되고, 내부에검출용액(detection solution)이담겨지는검출공간; 상기시료공간과상기검출공간을연결하는채널; 및상기채널에위치하여, 상기혼합용액및 검출용액의혼합을방지하며, 외부압력에의해서상기채널을개방하는공기밸브;를포함하는수직형정지액체상랩온어칩에관한것이다.
Abstract translation: 本发明涉及一种垂直固定液相实验室，包括：样品空间，其中存储含有颗粒的反应物和含有分析物的样品的混合溶液; 检测空间，其安装成与所述样本空间的下部连接，其中所述检测溶液存储在所述检测空间中; 将样本空间与检测空间连接的通道; 以及布置在通道上的空气阀，防止混合溶液和检测溶液彼此混合，并且通过外部压力打开通道。 根据本发明，由于在样本空间和检测空间之间包括空气阀，因此可以分别防止样本空间和检测空间中包含的溶液彼此混合。
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公开(公告)号：KR1020160079958A
公开(公告)日：2016-07-07
申请号：KR1020140190365
申请日：2014-12-26
Applicant: 강릉원주대학교산학협력단
Inventor： 최석정
IPC: B01L3/00
CPC classification number: B01L3/5027 , B01L3/50273
Abstract: 본발명은자성입자를포함하는정지액체상랩온어칩분석장치에있어서, 상기랩온어칩을수용하는데크플레이트; 상기데크플레이트를받쳐주는적어도 4개의기둥; 및상기기둥과상기데크플레이트사이에위치하여, 상기데크플레이트에진동을가하는데크진동모터;를포함하며, 상기기둥의소정부분은플렉서블한재질의데크지주를포함하는것을특징으로하는정지액체상랩온어칩분석장치에관한것이다.
Abstract translation: 本发明涉及一种用于分析片上实验室中的固定液相的装置。 用于分析在片上实验室中含有磁性颗粒的固定液体的装置包括：容纳片上实验室的甲板; 支撑甲板的至少四列; 以及位于柱和甲板之间的甲板振动马达，并且振动甲板板。 柱的规定部分包括由柔性材料制成的甲板支撑件。 本发明的装置能够均匀地振动整个甲板板，从而实现有效的搅拌。
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公开(公告)号：KR1020140067418A
公开(公告)日：2014-06-05
申请号：KR1020120134618
申请日：2012-11-26
Applicant: 강릉원주대학교산학협력단
Inventor： 최석정
IPC: C07D207/12 , G01N33/553
Abstract: The present invention relates to a method for introducing N-hydroxysuccinimide group to a material surface and, more specifically, to a method for introducing N-hydroxysuccinimide group to a material surface which mixes a compound having the N-hydroxysuccinimide group with a lipid and introduces the same to the material surface. The method according to the present invention is simple and economical, thereby efficiency and reproducibility of receptor fixation can be enhanced. With such effects, an introduction of NHS group to the surface of a biosensor, a gas sensor, a chromatographic medium, a nanomaterial for drug delivery, a nanomaterial for diagnosing, and the like is made advantageous.
Abstract translation: 本发明涉及将N-羟基琥珀酰亚胺基团引入材料表面的方法，更具体地说，涉及将N-羟基琥珀酰亚胺基团引入材料表面的方法，该方法将具有N-羟基琥珀酰亚胺基团的化合物与脂质混合并引入 与材料表面相同。 根据本发明的方法简单经济，从而可以提高受体固定的效率和再现性。 具有这样的效果，NHS基团在生物传感器，气体传感器，色谱介质，药物输送用纳米材料，诊断用纳米材料等的表面上的引入是有利的。
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Patent Agency Ranking
公开(公告)号：KR1020130098647A
公开(公告)日：2013-09-05
申请号：KR1020120020333
申请日：2012-02-28
Applicant: 강릉원주대학교산학협력단
Inventor： 최석정
CPC classification number: G01N33/58
Abstract: PURPOSE: A functionalization method of solid surface is provided to form a stable lipid layer compared with a conventional method. CONSTITUTION: A functionalization method of solid surface using lipid comprises the steps of mixing lipid and a lipophilic anchor compound with a hydrophobic chain and a functional group which forms covalent bond with the solid surface. The method comprises the step of inducing polymerization of polymerization lipid by crosslinking after the polymerization lipid is introduced on the solid surface. The method comprises the steps of: mixing the lipophilic anchor compound with the polymerization lipid and introducing the solid surface; and polymerizing the polymerization lipid through crosslinking between the polymerization lipids.
Abstract translation: 目的：提供固体表面的功能化方法，与常规方法相比形成稳定的脂质层。 构成：使用脂质的固体表面的官能化方法包括将脂质和亲脂性锚化合物与疏水性链和与固体表面形成共价键的官能团混合的步骤。 该方法包括在聚合脂质引入固体表面之后通过交联诱导聚合脂质聚合的步骤。 该方法包括以下步骤：将亲脂性锚定化合物与聚合脂质混合并引入固体表面; 并通过聚合脂质之间的交联聚合聚合脂质。
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公开(公告)号：KR101157990B1
公开(公告)日：2012-06-25
申请号：KR1020100119993
申请日：2010-11-29
Applicant: 강릉원주대학교산학협력단 , 주식회사 아모그린텍
IPC: G01N33/569 , C12Q1/24
Abstract: PURPOSE: A method for detecting an analyte is provided to detect various materials with a sensor chip and to detect specific material from a sample. CONSTITUTION: A method for detecting an analyte comprises: a step of inducing conjugation of a receptor and analyte(8a) in a sample to form an analyte-conjugated bacteria-receptor complex(9); a step of filtering the bacteria-receptor complex and isolating bacteria-receptor complex using a microfilter; and a step of detecting bacteria-receptor complex using a bio-sensor chip.
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公开(公告)号：KR1020120000392A
公开(公告)日：2012-01-02
申请号：KR1020100060738
申请日：2010-06-25
Applicant: 강릉원주대학교산학협력단
Abstract: PURPOSE: A peptide which binds to an antibody is provided to be used for immunoassay and antibody purification and to reduce costs. CONSTITUTION: A peptide which binds to an antibody has KHRFNKD, PIISRAT, SKPLHKM, AAPSPRN, or PLSPSQE. A method for preparing the peptide comprises: a step of searching for a phage-peptide library with seven random amino acids; a step of adding the phage-peptide library to a culture dish on which an antibody is conjugated and repetitively biopanning; and a step of detecting base sequences of a peptide with high frequency.
Abstract translation: 目的：提供与抗体结合的肽，用于免疫测定和抗体纯化，降低成本。 构成：与抗体结合的肽具有KHRFNKD，PIISRAT，SKPLHKM，AAPSPRN或PLSPSQE。 制备肽的方法包括：用七个随机氨基酸搜索噬菌体肽文库的步骤; 将噬菌体肽文库加入到其上结合抗体并重复生物淘选的培养皿中的步骤; 以及检测高频肽的碱基序列的步骤。
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公开(公告)号：KR100965244B1
公开(公告)日：2010-06-25
申请号：KR1020070138373
申请日：2007-12-27
Applicant: 강릉원주대학교산학협력단
Abstract: 본 발명은 리포솜을 이용한 바이오센서 또는 가스센서의 제조방법에 관한 것으로, 자기조립단분자층에 수용체 고정용 작용기를 포함하는 지질단일층을 결합시킴으로써, 본 발명의 제조방법을 이용시 기존방법에 비해 간편하고 시간이 단축될 뿐만 아니라 수용체 고정에 있어서 효율 및 재현성이 높은 효과가 있다. 또한 여러 시약을 사용할 필요가 없고 간단하여 화학비전공자들도 쉽게 제조할 수 있으므로 매우 뛰어난 발명이라 할 것이다.
바이오센서, 가스센서, 리포솜, 자기조립단분자층, 지질층-
公开(公告)号：KR101643477B1
公开(公告)日：2016-07-28
申请号：KR1020140190365
申请日：2014-12-26
Applicant: 강릉원주대학교산학협력단
Inventor： 최석정
IPC: B01L3/00
Abstract: 본발명은자성입자를포함하는정지액체상랩온어칩분석장치에있어서, 상기랩온어칩을수용하는데크플레이트; 상기데크플레이트를받쳐주는적어도 4개의기둥; 및상기기둥과상기데크플레이트사이에위치하여, 상기데크플레이트에진동을가하는데크진동모터;를포함하며, 상기기둥의소정부분은플렉서블한재질의데크지주를포함하는것을특징으로하는정지액체상랩온어칩분석장치에관한것이다.
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