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公开(公告)号：KR101437577B1
公开(公告)日：2014-09-05
申请号：KR1020120131968
申请日：2012-11-20
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 흑염소 반추위 미생물 유래의 섬유소분해효소 cel-KG27 유전자 및 이의 용도에 관한 것으로, 보다 구체적으로 흑염소 반추위 미생물로부터 선별한 신규한 섬유소분해효소 유전자인 cel-KG27 유전자 및 이의 단백질 산물을 제공하고, 이를 이용하여 사료첨가제, 세제 조성물 및 바이오연료를 제조하는 방법에 관한 것이다.
본 발명에 따르면, 흑염소 반추위 미생물로부터 신규한 섬유소분해효소 유전자인 cel-KG27 유전자를 선별할 수 있으며, 이를 통해 섬유소분해효소 cel-KG27 유전자 및 단백질 산물을 제공함으로써 저가의 사료 첨가제 개발에 이용할 수 있고, 섬유 유연제 및 세제 개발뿐만 아니라 풍부한 자원인 섬유소를 분해하여 바이오연료의 생산에도 적극적으로 이용할 수 있다.-
42.发明公开
公开(公告)号：KR1020140064567A
公开(公告)日：2014-05-28
申请号：KR1020120132012
申请日：2012-11-20
Applicant: 대한민국(농촌진흥청장)
Abstract: The present invention relates to a fibrinogenase cel-KG21 gene derived from rumen microorganisms of a black goat and uses thereof, and more specifically, to a cel-KG21 gene which is a novel fibrinogenase gene selected from rumen microorganisms of a black goat and a method of providing protein products thereof and manufacturing feed additives, a detergent composition, and biofuel by using the same. According to the present invention, a cel-KG21 gene which is a novel fibrinogenase gene derived from the rumen microorganisms of a black goat can be selected, and by providing the fibrinogenase cel-KG21 gene and protein products therethrough, they can be used for developing low-priced feed additives, softener, and detergent and actively used for production of biofuel by decomposing fibrins which are rich sources.
Abstract translation: 本发明涉及一种来源于黑山羊瘤胃微生物的纤维蛋白原酶cel-KG21基因及其用途，更具体地说，涉及一种选自黑山羊瘤胃微生物的新型纤维蛋白原酶基因的cel-KG21基因 提供其蛋白质产物和制造饲料添加剂，洗涤剂组合物和使用它们的生物燃料。 根据本发明，可以选择作为来自黑山羊的瘤胃微生物的新型纤维蛋白原酶基因的cel-KG21基因，通过提供纤维蛋白原酶-GC21基因和蛋白质产物，可以将其用于显影 低价饲料添加剂，软化剂和洗涤剂，并通过分解富集的纤维素积极地用于生产生物燃料。
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43.发明公开
公开(公告)号：KR1020140064564A
公开(公告)日：2014-05-28
申请号：KR1020120132007
申请日：2012-11-20
Applicant: 대한민국(농촌진흥청장)
Abstract: The present invention relates to a fibrinogenase cel-KG44 gene derived from rumen microorganisms of a black goat and uses thereof, and more specifically, to a cel-KG44 gene which is a novel fibrinogenase gene selected from rumen microorganisms of a black goat and a method of providing protein products thereof and manufacturing feed additives, a detergent composition, and biofuel by using the same. According to the present invention, a cel-KG44 gene which is a novel fibrinogenase gene derived from the rumen microorganisms of a black goat can be selected, and by providing the fibrinogenase cel-KG44 gene and protein products therethrough, they can be used for developing low-priced feed additives, softener, and detergent and actively used for production of biofuel by decomposing fibrins which are rich sources.
Abstract translation: 本发明涉及来自黑山羊瘤胃微生物的纤维蛋白原酶cel-KG44基因及其用途，更具体地说，涉及一种选自黑山羊瘤胃微生物的新型纤维蛋白原酶基因cel-KG44基因 提供其蛋白质产物和制造饲料添加剂，洗涤剂组合物和使用它们的生物燃料。 根据本发明，可以选择作为来自黑山羊的瘤胃微生物的新型纤维蛋白原酶基因的cel-KG44基因，通过提供纤维蛋白原酶cel-KG44基因和蛋白质产物，可以用于显影 低价饲料添加剂，软化剂和洗涤剂，并通过分解富集的纤维素积极地用于生产生物燃料。
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公开(公告)号：KR1020130068010A
公开(公告)日：2013-06-25
申请号：KR1020110135116
申请日：2011-12-15
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N9/2437 , A23K10/14 , C11D3/38645 , C12N15/67 , C12P1/00 , Y02E50/10
Abstract: PURPOSE: A product of cel-KG52, cellulase, is provided to be used as a feed additive, to produce a detergent and biofuel, and to be used in industrial fields. CONSTITUTION: A cellulase protein derived from a ruminant stomach microorganism of a black goat has an amino acid sequence of sequence number 2. A gene encodes the cellulase protein. A recombinant vector contains the gene. A host cell is transformed by the recombinant vector. A method for producing a large amount of cellulase from the host cell comprises a step of transducing the recombinant vector to the host cell, and overexpressing cellulase.
Abstract translation: 目的：提供cel-KG52，纤维素酶的产品，用作饲料添加剂，生产洗涤剂和生物燃料，并用于工业领域。 构成：来自黑山羊的反刍动物胃微生物的纤维素酶蛋白具有序列号2的氨基酸序列。基因编码纤维素酶蛋白。 重组载体含有该基因。 宿主细胞被重组载体转化。 从宿主细胞产生大量纤维素酶的方法包括将重组载体转导到宿主细胞和过表达纤维素酶的步骤。
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公开(公告)号：KR1020130068009A
公开(公告)日：2013-06-25
申请号：KR1020110135115
申请日：2011-12-15
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N9/2437 , A23K10/14 , C11D3/38645 , C12N15/67 , C12P1/00 , Y02E50/10
Abstract: PURPOSE: A product of cel8-KG47 gene, endoglucanase, which is acquired by searching cellulose decomposition enzyme from a ruminant stomach microorganism of a black goat is provided to be used for a feed additive and a detergent composition, and to develop biofuel with high efficiency. CONSTITUTION: An endoglucanase protein derived from a ruminant stomach microorganism of a black goat has an amino acid sequence of sequence number 2. A gene encodes the endoglucanase protein. A recombinant vector contains the gene. A host cell is transformed by the recombinant vector. A method for producing a large amount of endoglucanase from the host cell comprises: a step of transducing the recombinant vector to the host cell; and a step of overexpressing endoglucanase.
Abstract translation: 目的：提供一种通过从黑山羊的反刍动物胃微生物中搜索纤维素分解酶获得的cel8-KG47基因，内切葡聚糖酶的产物，用于饲料添加剂和洗涤剂组合物，并以高效率开发生物燃料 。 构成：来自黑山羊的反刍动物胃微生物的内切葡聚糖酶蛋白具有序列号2的氨基酸序列。基因编码内切葡聚糖酶蛋白质。 重组载体含有该基因。 宿主细胞被重组载体转化。 从宿主细胞产生大量内切葡聚糖酶的方法包括：将重组载体转导至宿主细胞的步骤; 以及过表达内切葡聚糖酶的步骤。
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Patent Agency Ranking
公开(公告)号：KR1020130068008A
公开(公告)日：2013-06-25
申请号：KR1020110135114
申请日：2011-12-15
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N9/248 , A23K10/14 , C11D3/38636 , C12N15/67 , C12P7/649
Abstract: PURPOSE: A product of cel10-KG42 gene, endoxylanase, which is obtained by searching cellulose decomposing enzymes from a ruminant stomach microorganism of a black goat is provided to be used in a feed additive and a detergent composition, and to develop biofuel with high efficiency. CONSTITUTION: An endoxylanase protein derived from a ruminant stomach microorganism of a black goat has an amino acid sequence of sequence number 2. The gene encodes endoxylanase protein. A recombinant vector contains the gene. A host cell is transformed by the recombinant vector. A method for producing a large amount of endoxylanase from the host cell comprises a step of transducing the recombinant vector to the host cell and overexpressing endoxylanse.
Abstract translation: 目的：提供一种通过从黑山羊的反刍动物胃微生物中搜索纤维素分解酶而获得的cel10-KG42基因的内切木聚糖酶，用于饲料添加剂和洗涤剂组合物，并以高效率开发生物燃料 。 构成：来自黑山羊的反刍动物胃微生物的内切木聚糖酶蛋白具有序列号2的氨基酸序列。该基因编码内切木聚糖酶蛋白。 重组载体含有该基因。 宿主细胞被重组载体转化。 从宿主细胞产生大量内切木聚糖酶的方法包括将重组载体转导至宿主细胞并过量表达内生木聚糖的步骤。
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公开(公告)号：KR1020130068002A
公开(公告)日：2013-06-25
申请号：KR1020110135108
申请日：2011-12-15
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N9/2437 , A23K10/14 , C11D3/38645 , C12N15/67 , C12P1/00 , Y02E50/10
Abstract: PURPOSE: A product of cel-KG20, cellulase, is provided to be used as a feed additive, to produce a detergent and a biofuel, and to be industrially used. CONSTITUTION: A cellulase protein derived from a ruminant stomach microorganism of a black goat contains a microorganism sequence of sequence number 2. A gene encodes the cellulase protein. A recombinant vector contains the gene. A host cell is transformed by the recombinant vector. A method for producing a large amount of cellulase from the host cell comprises a step of transducing the recombinant vector to the host cell and overexpressing cellulase.
Abstract translation: 目的：提供cel-KG20，纤维素酶的产品，用作饲料添加剂，生产洗涤剂和生物燃料，并在工业上使用。 构成：衍生自黑山羊的反刍动物胃微生物的纤维素酶蛋白含有序列号2的微生物序列。基因编码纤维素酶蛋白。 重组载体含有该基因。 宿主细胞被重组载体转化。 从宿主细胞产生大量纤维素酶的方法包括将重组载体转导到宿主细胞并过度表达纤维素酶的步骤。
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公开(公告)号：KR1020150057201A
公开(公告)日：2015-05-28
申请号：KR1020130140225
申请日：2013-11-18
Applicant: 대한민국(농촌진흥청장)
Abstract: 본발명은흑염소반추위미생물유래의섬유소분해효소 cel10-KG88 유전자및 이의용도에관한것으로, 보다구체적으로흑염소반추위미생물로부터선별한신규한섬유소분해효소유전자인 cel10-KG88 유전자및 이의단백질산물을제공하고, 이를이용하여사료첨가제, 세제조성물및 바이오연료를제조하는방법에관한것이다. 본발명에따르면, 흑염소반추위미생물로부터신규한섬유소분해효소유전자인 cel10-KG88 유전자를선별할수 있으며, 이를통해섬유소분해효소 cel10-KG88 유전자및 단백질산물을제공함으로써저가의사료첨가제개발에이용할수 있고, 섬유유연제및 세제개발뿐만아니라풍부한자원인섬유소를분해하여바이오연료의생산에도적극적으로이용할수 있다.
Abstract translation: 本发明涉及来自黑山羊的瘤胃微生物的纤维素cel10-KG88基因及其用途，更具体地说，涉及通过使用cel10-KG88制造饲料添加剂，洗涤剂组合物和生物燃料的方法 基因，其是选自黑山羊的瘤胃微生物的新型纤维素基因及其蛋白质产物。 根据本发明，可以从黑山羊的瘤胃微生物中分离出作为新型纤维素基因的cel10-KG88基因，由此提供纤维素cel10-KG88基因和蛋白质产物，用于开发廉价的饲料 添加剂，不仅可以开发柔软剂和洗涤剂，还可以通过分解纤维素来生产生物燃料，这是一种丰富的资源。
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公开(公告)号：KR1020150057195A
公开(公告)日：2015-05-28
申请号：KR1020130140214
申请日：2013-11-18
Applicant: 대한민국(농촌진흥청장)
Abstract: 본발명은흑염소반추위미생물유래의섬유소분해효소 cel10-CBM4-KG91 유전자및 이의용도에관한것으로, 보다구체적으로흑염소반추위미생물로부터선별한신규한섬유소분해효소유전자인 cel10-CBM4-KG91 유전자및 이의단백질산물을제공하고, 이를이용하여사료첨가제, 세제조성물및 바이오연료를제조하는방법에관한것이다. 본발명에따르면, 흑염소반추위미생물로부터신규한섬유소분해효소유전자인 cel10-CBM4-KG91 유전자를선별할수 있으며, 이를통해섬유소분해효소 cel10-CBM4-KG91 유전자및 단백질산물을제공함으로써저가의사료첨가제개발에이용할수 있고, 섬유유연제및 세제개발뿐만아니라풍부한자원인섬유소를분해하여바이오연료의생산에도적극적으로이용할수 있다.
Abstract translation: 本发明涉及来自黑山羊瘤胃微生物的纤维素cel10-CBM4-KG91基因及其用途，更具体地说，涉及通过使用cel10制备饲料添加剂，洗涤剂组合物和生物燃料的方法 -CBM4-KG91基因，其是选自黑山羊的瘤胃微生物的新型纤维素基因及其蛋白质产物。 根据本发明，可以从黑山羊的瘤胃微生物中分离出作为新型纤维素基因的cel10-CBM4-KG91基因，因此提供了纤维素cel10-CBM4-KG91基因和蛋白质产物用于开发 一种廉价的饲料添加剂，不仅可以开发软化剂和洗涤剂，还可以通过分解纤维素来生产生物燃料，这是一种丰富的资源。
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公开(公告)号：KR101483584B1
公开(公告)日：2015-01-19
申请号：KR1020110135107
申请日：2011-12-15
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 흑염소 반추위 미생물 유래의 셀룰라아제 (cellulase) 단백질, 상기 셀룰라아제 단백질을 코딩하는 유전자, 상기 유전자를 포함하는 재조합 벡터, 상기 재조합 벡터로 형질전환된 숙주세포, 상기 셀룰라아제 단백질에 대한 항체, 상기 재조합 벡터를 숙주세포에 도입하여 셀룰라아제를 과발현하는 단계를 포함하는 숙주세포에서 셀룰라아제를 대량생산하는 방법, 상기 방법에 의해 제조된 재조합 셀룰라아제 단백질, 상기 셀룰라아제 단백질 또는 상기 재조합 셀룰라아제 단백질을 유효성분으로 포함하는 셀룰로오스 분해 증진용 사료 첨가제 또는 세제 조성물 및 바이오매스 물질에 상기 셀룰라아제 단백질 또는 상기 재조합 셀룰라아제 단백질을 첨가하여 가수분해시키는 단계를 포함하는 바이오연료(Biofuel)의 제조방법을 제공한다.
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