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公开(公告)号：KR101437529B1
公开(公告)日：2014-09-05
申请号：KR1020120132009
申请日：2012-11-20
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 흑염소 반추위 미생물 유래의 섬유소분해효소 cel-KG48 유전자 및 이의 용도에 관한 것으로, 보다 구체적으로 흑염소 반추위 미생물로부터 선별한 신규한 섬유소분해효소 유전자인 cel-KG48 유전자 및 이의 단백질 산물을 제공하고, 이를 이용하여 사료첨가제, 세제 조성물 및 바이오연료를 제조하는 방법에 관한 것이다.
본 발명에 따르면, 흑염소 반추위 미생물로부터 신규한 섬유소분해효소 유전자인 cel-KG48 유전자를 선별할 수 있으며, 이를 통해 섬유소분해효소 cel-KG48 유전자 및 단백질 산물을 제공함으로써 저가의 사료 첨가제 개발에 이용할 수 있고, 섬유 유연제 및 세제 개발뿐만 아니라 풍부한 자원인 섬유소를 분해하여 바이오연료의 생산에도 적극적으로 이용할 수 있다.-
42.发明公开
公开(公告)号：KR1020140064577A
公开(公告)日：2014-05-28
申请号：KR1020120132031
申请日：2012-11-20
Applicant: 대한민국(농촌진흥청장)
Abstract: The present invention relates to a fibrinogenase cel-KG51 gene derived from rumen microorganisms of a black goat and uses thereof, and more specifically, to a cel-KG51 gene which is a novel fibrinogenase selected from rumen microorganisms of a black goat and a method of providing protein products thereof and manufacturing feed additives, a detergent composition, and biofuel by using the same. According to the present invention, a cel-KG51 gene which is a novel fibrinogenase gene derived from the rumen microorganisms of a black goat can be selected, and by providing the fibrinogenase cel-KG51 gene and protein products therethrough, they can be used for developing low-priced feed additives, softener, and detergent and actively used for production of biofuel by decomposing fibrins which are rich sources.
Abstract translation: 本发明涉及一种来自黑山羊瘤胃微生物的纤维蛋白原酶cel-KG51基因及其用途，更具体地说，涉及一种选自黑山羊瘤胃微生物的新型纤维蛋白原酶的cel-KG51基因， 通过使用它们提供蛋白质产物和制造饲料添加剂，洗涤剂组合物和生物燃料。 根据本发明，可以选择作为来自黑山羊的瘤胃微生物的新型纤维蛋白原酶基因的cel-KG51基因，通过提供纤维蛋白原酶cel-KG51基因和蛋白质产物，可以用于显影 低价饲料添加剂，软化剂和洗涤剂，并通过分解富集的纤维素积极地用于生产生物燃料。
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43.发明公开
公开(公告)号：KR1020140064566A
公开(公告)日：2014-05-28
申请号：KR1020120132011
申请日：2012-11-20
Applicant: 대한민국(농촌진흥청장)
Abstract: The present invention relates to a fibrinogenase cel-KG55 gene derived from rumen microorganisms of a black goat and uses thereof, and more specifically, to a cel-KG55 gene which is a novel fibrinogenase gene selected from rumen microorganisms of a black goat and a method of providing protein products thereof and manufacturing feed additives, a detergent composition, and biofuel by using the same. According to the present invention, a cel-KG55 gene which is a novel fibrinogenase gene derived from the rumen microorganisms of a black goat can be selected, and by providing the fibrinogenase cel-KG55 gene and protein products therethrough, they can be used for developing low-priced feed additives, softener, and detergent and actively used for production of biofuel by decomposing fibrins which are rich sources.
Abstract translation: 本发明涉及一种来自黑山羊瘤胃微生物的纤维蛋白原酶cel-KG55基因及其用途，更具体地说，涉及一种选自黑山羊瘤胃微生物的新型纤维蛋白原酶基因cel-KG55基因 提供其蛋白质产物和制造饲料添加剂，洗涤剂组合物和使用它们的生物燃料。 根据本发明，可以选择作为来自黑山羊的瘤胃微生物的新型纤维蛋白原酶基因的cel-KG55基因，通过提供纤维蛋白原酶cel-KG55基因和蛋白质产物，可以用于开发 低价饲料添加剂，软化剂和洗涤剂，并通过分解富集的纤维素积极地用于生产生物燃料。
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44.发明公开
公开(公告)号：KR1020140064561A
公开(公告)日：2014-05-28
申请号：KR1020120132001
申请日：2012-11-20
Applicant: 대한민국(농촌진흥청장)
Abstract: The present invention relates to a fibrinogenase cel-KG37 gene derived from rumen microorganisms of a black goat and uses thereof, and more specifically, to a cel-KG37 gene which is a novel fibrinogenase gene selected from rumen microorganisms of a black goat and a method of providing protein products thereof and manufacturing feed additives, a detergent composition, and biofuel by using the same. According to the present invention, a cel-KG37 gene which is a novel fibrinogenase gene derived from the rumen microorganisms of a black goat can be selected, and by providing the fibrinogenase cel-KG37 gene and protein products therethrough, they can be used for developing low-priced feed additives, softener, and detergent and actively used for production of biofuel by decomposing fibrins which are rich sources.
Abstract translation: 本发明涉及一种来自黑山羊瘤胃微生物的纤维蛋白原酶cel-KG37基因及其用途，更具体地说，涉及一种选自黑山羊瘤胃微生物的新型纤维蛋白原酶基因cel-KG37基因 提供其蛋白质产物和制造饲料添加剂，洗涤剂组合物和使用它们的生物燃料。 根据本发明，可以选择作为来自黑山羊的瘤胃微生物的新型纤维蛋白原酶基因的cel-KG37基因，通过提供纤维蛋白原酶cel-KG37基因和蛋白质产物，可以用于显影 低价饲料添加剂，软化剂和洗涤剂，并通过分解富集的纤维素积极地用于生产生物燃料。
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45.发明公开
公开(公告)号：KR1020140064535A
公开(公告)日：2014-05-28
申请号：KR1020120131957
申请日：2012-11-20
Applicant: 대한민국(농촌진흥청장)
Abstract: The present invention relates to a fibrinogenase cel-KG11 gene derived from rumen microorganisms of a black goat and uses thereof, and more specifically, to a cel-KG11 gene which is a novel fibrinogenase selected from rumen microorganisms of a black goat and a method of providing protein products thereof and manufacturing feed additives, a detergent composition, and biofuel by using the same. According to the present invention, by selecting a cel-KG11 gene which is a novel fibrinogenase gene derived from the rumen microorganisms of a black goat and providing a fibrinogenase cel-KG11 gene and protein products therethrough, it can be used for developing low-priced feed additives, softener, and detergent and actively used for production of biofuel by decomposing fibrins which are rich sources.
Abstract translation: 本发明涉及来自黑山羊瘤胃微生物的纤维蛋白原酶cel-KG11基因及其用途，更具体地说，涉及一种选自黑山羊瘤胃微生物的新型纤维蛋白原酶的cel-KG11基因， 通过使用它们提供蛋白质产物和制造饲料添加剂，洗涤剂组合物和生物燃料。 根据本发明，通过选择作为来自黑山羊的瘤胃微生物的新型纤维蛋白原酶基因的cel-KG11基因，通过其提供纤维蛋白原酶cel-KG11基因和蛋白质产物，可以用于开发低价位 饲料添加剂，软化剂和洗涤剂，并通过分解富集的纤维素积极地用于生产生物燃料。
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Patent Agency Ranking
公开(公告)号：KR1020130068003A
公开(公告)日：2013-06-25
申请号：KR1020110135109
申请日：2011-12-15
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N9/2437 , A23K10/14 , C07K16/40 , C11D3/38645 , C12N15/67 , C12P1/00 , Y02E50/10
Abstract: PURPOSE: A Cel3-KG22 product, cellulase, is provided to be used in a feed additive and a detergent composition by finding a cellulase gene from a ruminant stomach microorganism of a black goat, to develop biofuel. CONSTITUTION: A cellulase protein derived from a ruminant stomach microorganism has an amino acid sequence of sequence number 2. A gene encodes the cellulase protein. A recombinant vector contains the gene. The host cell is transformed by the recombinant vector. A method for producing a large amount of cellulase from the host cell comprises a step of transducing the recombinant vector to the host cell and overexpressing cellulase.
Abstract translation: 目的：通过从黑山羊的反刍动物胃微生物中发现纤维素酶基因来开发生物燃料，提供了Cel3-KG22产品纤维素酶用于饲料添加剂和洗涤剂组合物。 构成：源自反刍动物胃微生物的纤维素酶蛋白具有序列号2的氨基酸序列。基因编码纤维素酶蛋白。 重组载体含有该基因。 通过重组载体转化宿主细胞。 从宿主细胞产生大量纤维素酶的方法包括将重组载体转导到宿主细胞并过度表达纤维素酶的步骤。
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公开(公告)号：KR1020130068001A
公开(公告)日：2013-06-25
申请号：KR1020110135107
申请日：2011-12-15
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N9/2437 , A23K10/14 , C11D3/38645 , C12N15/67 , C12P1/00 , Y02E50/10
Abstract: PURPOSE: A protein of cel-KG19, cellulase, is provided to be used as a feed additive and to produce a detergent and biofuel. CONSTITUTION: A cellulase protein derived from a ruminant stomach microorganism of a black goat has an amino acid sequence of sequence number 2. A gene encodes the cellulase protein. A recombinant vector contains the gene. A host cell is transformed by the recombinant vector. A method for producing a large amount of cellulase in the host cell comprises a step of tranducing the recombinant vector into the host cell and overexpressing cellulase.
Abstract translation: 目的：提供cel-KG19蛋白质纤维素酶，用作饲料添加剂，生产洗涤剂和生物燃料。 构成：来自黑山羊的反刍动物胃微生物的纤维素酶蛋白具有序列号2的氨基酸序列。基因编码纤维素酶蛋白。 重组载体含有该基因。 宿主细胞被重组载体转化。 用于在宿主细胞中产生大量纤维素酶的方法包括将重组载体转染到宿主细胞中并过度表达纤维素酶的步骤。
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公开(公告)号：KR102019998B1
公开(公告)日：2019-09-10
申请号：KR1020180070473
申请日：2018-06-19
Applicant: 대한민국(농촌진흥청장)
IPC: C12Q1/6888 , C12Q1/6837 , G01N33/68
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公开(公告)号：KR101479712B1
公开(公告)日：2015-01-08
申请号：KR1020110135110
申请日：2011-12-15
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 흑염소 반추위 미생물 유래의 엔도글루카나아제 (endo-glucanase) 단백질, 상기 엔도글루카나아제 단백질을 코딩하는 유전자, 상기 유전자를 포함하는 재조합 벡터, 상기 재조합 벡터로 형질전환된 숙주세포, 상기 엔도글루카나아제 단백질 대한 항체, 상기의 재조합 벡터를 숙주세포에 도입하여 엔도글루카나아제를 과발현하는 단계를 포함하는 숙주세포에서 엔도글루카나아제를 대량생산하는 방법, 상기 방법에 의해 제조된 재조합 엔도글루카나아제 단백질, 상기 엔도글루카나아제 단백질 또는 상기 재조합 엔도글루카나아제 단백질을 유효성분으로 포함하는 글루칸 분해 증진용 사료 첨가제 또는 세제 조성물 및 바이오매스 물질에 상기 엔도글루카나아제 단백질 또는 상기 재조합 엔도글루카나아제 단백질을 첨가하여 가수분해시키는 단계를 포함하는 바이오연료 (Biofuel)의 제조 방법을 제공한다.
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公开(公告)号：KR101479704B1
公开(公告)日：2015-01-08
申请号：KR1020110135105
申请日：2011-12-15
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 흑염소 반추위 미생물 유래의 엔도글루카나아제 (endo-glucanase) 단백질, 상기 엔도글루카나아제 단백질을 코딩하는 유전자, 상기 유전자를 포함하는 재조합 벡터, 상기 재조합 벡터로 형질전환된 숙주세포, 상기 엔도글루카나아제 단백질 대한 항체, 상기의 재조합 벡터를 숙주세포에 도입하여 엔도글루카나아제를 과발현하는 단계를 포함하는 숙주세포에서 엔도글루카나아제를 대량생산하는 방법, 상기 방법에 의해 제조된 재조합 엔도글루카나아제 단백질, 상기 엔도글루카나아제 단백질 또는 상기 재조합 엔도글루카나아제 단백질을 유효성분으로 포함하는 글루칸 분해 증진용 사료 첨가제 또는 세제 조성물 및 바이오매스 물질에 상기 엔도글루카나아제 단백질 또는 상기 재조합 엔도글루카나아제 단백질을 첨가하여 가수분해시키는 단계를 포함하는 바이오연료 (Biofuel)의 제조 방법을 제공한다.
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