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公开(公告)号：KR100481390B1
公开(公告)日：2005-04-11
申请号：KR1020030015966
申请日：2003-03-14
Applicant: 한국과학기술원
IPC: C07K19/00
Abstract: 본 발명은 폴리하이드록시알카노이트 (PHA) 마이크로스피어에 목적단백질 또는 목적리간드가 부착되어 있는 결합체에 있어서, 목적단백질 또는 목적리간드와 PHA 마이크로스피어 사이에 PHA 분해효소의 기질결합 영역이 링커로 삽입되어 있어 목적단백질 또는 목적리간드가 상기 링커를 통해 PHA 마이크로스피어에 연결되어 있는 결합체에 관한 것이다. 본 발명에서 제공되는 PHA 마이크로스피어의 결합체는 목적단백질-반응단백질간의 반응, 목적단백질-반응리간드간의 반응, 목적리간드-반응단백질간의 반응 또는 목적리간드-반응리간드 간의 반응을 효율적으로 검출하는 데 유용하다.
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公开(公告)号：KR1020040081238A
公开(公告)日：2004-09-21
申请号：KR1020030015966
申请日：2003-03-14
Applicant: 한국과학기술원
IPC: C07K19/00
CPC classification number: C07K17/02 , C12N9/16 , C12Y301/01
Abstract: PURPOSE: A conjugate of PHA microsphere and target protein or ligand and a method for detecting reactions are provided, thereby stably and high efficiently detecting a reaction between target protein and reaction protein, a reaction between target protein and reaction ligand, a reaction between target ligand and reaction protein or a reaction between target ligand and reaction ligand, and cheaply producing the PHA microsphere. CONSTITUTION: The conjugate of PHA microsphere and target protein or ligand contains a substrate-binding site of PHA depolymerase as a linker between the PHA microsphere and target protein or ligand, wherein the target protein is fused in the substrate-binding site of PHA depolymerase; the substrate-binding site of PHA depolymerase is fused with the C-terminal, N-terminal or intermediate of the N-C terminals of the target protein; the target protein is an enzyme or an antibody; the target ligand is low molecular weight compound, nucleic acid, peptide, carbohydrate, fatty acid or lipid which reacts with protein or peptide; the PHA is polymer, copolymer or polymer blend containing C3-C12 monomer; and the substrate-binding site of PHA depolymerase is derived from Alcaligenes faecalis. The method for detecting reactions comprises using the conjugate of PHA microsphere and target protein or ligand, wherein the reaction is detected by using fluorescence-labeled antibody.
Abstract translation: 目的：提供PHA微球和靶蛋白或配体的缀合物和检测反应的方法，从而稳定高效地检测靶蛋白与反应蛋白之间的反应，靶蛋白与反应配体之间的反应，靶配体之间的反应 和反应蛋白质或靶配体与反应配体之间的反应，并且廉价地生产PHA微球。 构成：PHA微球和靶蛋白或配体的缀合物含有PHA解聚酶的底物结合位点，作为PHA微球和靶蛋白或配体之间的接头，其中靶蛋白融合在PHA解聚酶的底物结合位点中; PHA解聚酶的底物结合位点与靶蛋白的N末端的C-末端，N-末端或中间体融合; 靶蛋白是酶或抗体; 靶配体是与蛋白质或肽反应的低分子量化合物，核酸，肽，碳水化合物，脂肪酸或脂质; PHA是含有C 3 -C 12单体的聚合物，共聚物或聚合物共混物; PHA解聚酶的底物结合位点来源于粪产碱杆菌。 检测反应的方法包括使用PHA微球和靶蛋白或配体的缀合物，其中通过使用荧光标记的抗体检测反应。
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公开(公告)号：KR1020040069386A
公开(公告)日：2004-08-06
申请号：KR1020030005730
申请日：2003-01-29
Applicant: 한국과학기술원
IPC: G01N33/543
Abstract: PURPOSE: A protein chip using substrate specificity of polyhydroxyalkanonate depolymerase is provided, thereby inhibiting nonspecific protein binding, so that cost for production of the protein chip can be reduced. CONSTITUTION: The protein chip using substrate specificity of polyhydroxyalkanonate depolymerase comprises a substrate, PHA(polyhydroxyalkanonate) fixed on the substrate, a substrate binding domain of PHA depolymerase, and a fusion protein of a target protein, wherein the substrate is glass slide; the PHA is spin coated on the substrate; the PHA has 4 to 12 of carbon number; the binding domain of PHA depolymerase is fused with the N-terminal, C-terminal or the intermediate of N-C of a target protein; and the binding domain of PHA depolymerase is isolated from Alcaligenes faecalis.
Abstract translation: 目的：提供使用聚羟基链烷酸酯解聚酶的底物特异性的蛋白质芯片，从而抑制非特异性蛋白质结合，从而可以降低生产蛋白质芯片的成本。 构成：使用聚羟基链烷酸酯解聚酶的底物特异性的蛋白质芯片包括固定在底物上的底物PHA（聚羟基卡那酸），PHA解聚酶的底物结合结构域和靶蛋白的融合蛋白，其中所述底物是玻璃载片; 将PHA旋涂在基材上; PHA有4到12个碳数; PHA解聚酶的结合域与靶蛋白的N末端，C-末端或中间体融合; 并且从粪产碱杆菌中分离出PHA解聚酶的结合结构域。
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公开(公告)号：KR1020040063058A
公开(公告)日：2004-07-12
申请号：KR1020030000464
申请日：2003-01-04
Applicant: 한국과학기술원
IPC: G01N33/543
CPC classification number: C40B30/04 , C07K1/1077 , C07K14/5759 , C12N9/0006 , G01N33/54353 , G01N33/6803
Abstract: PURPOSE: A protein chip for analyzing interaction between protein and substrate peptide therefor is provided, thereby rapidly and enormously analyzing interaction between protein and substrate peptide therefor by improving interaction between small molecular weight of peptides and large molecular weight of enzyme proteins, and between peptides and reactive antibodies. CONSTITUTION: The protein chip for analyzing interaction between protein and substrate peptide therefor is prepared by fixing a substrate peptide on a substrate such as aldehyde slide via a linker protein such as leptin or malic enzyme, wherein the substrate peptide is fused with the linker protein in a type of peptide monomer, dimmer of monomer-proline-monomer, or proline-linked polymer; and the peptide monomer is Camtide having the amino acid sequence set forth in SEQ ID NO: 1 or Ab1 having the amino acid sequence set forth in SEQ ID NO: 8. The method for analyzing interaction between protein and substrate peptide therefor comprises the steps of: adding a protein such as enzyme or antibody which specifically binds to the substrate peptide fixed on a protein chip into the protein chip; and detecting the interaction between the protein and substrate peptide therefor by using a fluorescence-labeled enzyme.
Abstract translation: 目的：提供用于分析蛋白质与底物肽之间相互作用的蛋白质芯片，从而通过改善肽与小分子量酶蛋白之间的相互作用，以及肽和底物肽之间的相互作用，快速而极大地分析蛋白质与底物肽之间的相互作用 反应性抗体。 构成：用于分析蛋白质和底物肽之间的相互作用的蛋白质芯片通过将底物肽通过接头蛋白如瘦素或苹果酸固定在底物如醛载体上而制备，其中底物肽与接头蛋白质融合 一类肽单体，单体 - 脯氨酸单体的二聚体，或脯氨酸交联的聚合物; 并且肽单体是具有SEQ ID NO：1所示氨基酸序列的Camtide或具有SEQ ID NO：8所示的氨基酸序列的Ab1。分析蛋白质与底物肽之间相互作用的方法包括以下步骤： ：将固定在蛋白质芯片上的底物肽特异性结合到蛋白质芯片中的蛋白质如酶或抗体; 并通过使用荧光标记的酶检测蛋白质和底物肽之间的相互作用。
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公开(公告)号：KR1020210001479A
公开(公告)日：2021-01-06
申请号：KR1020190077640
申请日：2019-06-28
Applicant: 한국과학기술원
Abstract: 본발명의실시예에따른미세유체장치제조용사출성형장치는일면에유체가흐르는유로가형성되고, 타면에상기유로와연통되어상기유로를통해흐르는유체를안내하여액적형태로낙하시키도록구성되는노즐부가형성되는미세액적생성장치등과같이표면에미세패턴이형성되는미세유체장치를제조하기위해사용되는미세유체장치제조용사출성형장치로서, 내측에적어도하나의채널을구비하는스탬프가설치되어, 사출시 사출물의일면에상기유로를형성하는제1 금형; 상기제1 금형에대향배치되고, 내측에사출시 상기사출물의타면에상기노즐부를형성하는사출홈이형성되는제2 금형; 및상기제1 금형의평면상에서상기스탬프의위치를 X축방향및 Y축방향으로조정가능한정렬부;를포함한다. 본발명의실시예에따르면, 스탬프가금형에설치된경우, 금형에충격을가하거나, 스탬프를재설치하지않고, 스탬프의외면을가압하여스탬프를 X축방향및 Y축방향으로미세하게이동시켜스탬프의위치를조정함으로써, 스탬프를정확한설정위치에배치시킬수 있음은물론, 작업시간을단축시킬수 있고, 금형의변형및 파손을예방할수 있다.
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