公开(公告)号：KR1020110018122A

公开(公告)日：2011-02-23

申请号：KR1020090075754

申请日：2009-08-17

Applicant: 서울대학교산학협력단 ,  부산대학교 산학협력단

Inventor： 서진호 ,  박용철 ,  곽수량 ,  박성훈 ,  스브라마니안모한라지 ,  첸라두라이라스나싱

IPC: C12P7/04 ,  C12P1/04 ,  C12P7/20

CPC classification number: C12P7/18 ,  C12N1/20 ,  C12N15/70 ,  C12P7/42 ,  C12Y101/01001 ,  C12Y101/01077 ,  C12Y102/01003 ,  C12Y402/0103

Abstract: PURPOSE: A method for simultaneously preparing 3-hydroxypropionic acid and 1,3-propandiol from glycerol using recombinant E.coli is provided. CONSTITUTION: A method for simultaneously preparing 3-hydroxypropionic acid and 1,3-propandiol is performed by culturing recombinant E.coli in a medium containing enough glycerol and glucose. The recombinant E.coli is obtained by transforming to expresses an enzyme which converts 3-hydroxypropion aldehyde to 3-hydroxypropionic acid.

Abstract translation: 目的：提供使用重组大肠杆菌从甘油同时制备3-羟基丙酸和1,3-丙二醇的方法。 构成：通过在含有足够的甘油和葡萄糖的培养基中培养重组大肠杆菌来进行同时制备3-羟基丙酸和1,3-丙二醇的方法。 通过转化来获得重组大肠杆菌，以表达将3-羟基丙醛转化为3-羟基丙酸的酶。

公开(公告)号：KR1020100088750A

公开(公告)日：2010-08-11

申请号：KR1020090007837

申请日：2009-02-02

Applicant: 서울대학교산학협력단

Inventor： 서진호 ,  이원흥

IPC: C12N15/52 ,  C12N15/63 ,  C12N15/70 ,  C12P19/00

Abstract: PURPOSE: A method for preparing GDP-fucose using recombinant E.coli is provided to overexpress an enzyme mediating GDP-fucose synthesis and to enhance yield of GDP-fucose. CONSTITUTION: A GDP-fucose is produced by culturing a recombinant E.coli. The recombinant E.coli overexpresses GDP-D-mannose-4,6-dehydratase, GDP-4-keto-6-deoxymannose 3,5-epimerase-4-reductase phosphomannomutase, mannose-1-phosphate guanyltransferase, and glucose-6-phosphate-dehydrogenase. The overexpression is controlled by induction promoter.

Abstract translation: 目的：提供使用重组大肠杆菌制备GDP-岩藻糖的方法，以过表达介导GDP-岩藻糖合成的酶，并提高GDP-岩藻糖的产量。 构成：通过培养重组大肠杆菌产生GDP-岩藻糖。 重组大肠杆菌过表达GDP-D-甘露糖-4,6-脱水酶，GDP-4-酮-6-脱氧甘露糖3.5-差向异构酶-4-还原酶磷酸化变位酶，甘露糖-1-磷酸鸟嘌呤转移酶和葡萄糖-6- 磷酸脱氢酶。 过表达受诱导启动子控制。