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公开(公告)号：KR1020140019615A
公开(公告)日：2014-02-17
申请号：KR1020120085960
申请日：2012-08-06
Applicant: 인하대학교 산학협력단
Abstract: The present invention relates to a solid acid catalyst and a method for preparing biodiesel using the same. The method for preparing biodiesel includes a step of preparing biochar and a step of adding acid to the prepared biochar and sintering at 250-600 deg. C for 1-5 seconds. [Reference numerals] (AA) Dunalliella skim cell; (BB) Dunalliella bio-charcoal; (CC) Acid solid catalyst
Abstract translation: 本发明涉及一种固体酸催化剂及使用其制备生物柴油的方法。 制备生物柴油的方法包括制备生物炭的步骤和向制备的生物炭中加入酸并在250-600度烧结的步骤。 C 1-5秒。 （参考数字）（AA）Dunalliella脱脂细胞; （BB）Dunalliella生物炭; （CC）酸性固体催化剂
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公开(公告)号：KR101316535B1
公开(公告)日：2013-10-15
申请号：KR1020110091817
申请日：2011-09-09
Applicant: 인하대학교 산학협력단
CPC classification number: Y02E50/13 , Y02P30/20 , Y02T50/678
Abstract: 본 발명은 미세조류로부터 다양한 종류의 바이오에너지를 순차적으로 생산하는 생산방법에 관한 것으로서, 배양 후 회수한 미세조류 균체를 건조하는 건조단계; 상기 건조된 균체를 파쇄하지 않고 추출용매로 추출하여, 고상-액상으로 분리하는 추출단계; 및 분리된 액상으로부터 긴사슬 탄화수소를 회수하는 회수단계를 포함하는 미세조류 균체로부터 긴사슬 탄화수소의 생산방법을 제공한다.
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公开(公告)号：KR1020130028823A
公开(公告)日：2013-03-20
申请号：KR1020110091818
申请日：2011-09-09
Applicant: 인하대학교 산학협력단
Abstract: PURPOSE: A method of manufacturing biodiesel is provided to manufacture biodiesel using cultured animal cell waste without a separate waste treatment process. CONSTITUTION: A method of manufacturing biodiesel from cultured animal cell salvage comprises a first extracting step of adding a polar organic solvent to the collected wet cultured animal cell salvage and stirring to extract lipid; a crude lipid yielding step of layer separating the supernatant and cell pellets and concentrating the supernatant to manufacture concentrate; a second extracting step of adding acid/alcohol solution to yielded crude lipid, refluxing and extracting with a non-polar organic solvent; and a separating step of collecting the non-polar solvent layer and concentrating under the reduced pressure to separating biodiesel. The animal cell is the CHO (Chinese hamster ovary) cell, the BHK (baby hamster kidney) cell, the HEK (human embryonic kidney) cell, and the NS 0 (myeloma cell line) cell. The animal cell is the CHO cell. The polar organic solvent is methanol, ethanol, isopropyl alcohol, acetone or methanol/chloroform solvent mixture. The polar organic solvent is applied at 1-10 times of volume to the volume of the cultured animal cell salvage. The mixing ratio of the methanol/chloroform mixed solution is 1:4 to 4:1. The acid/alcohol solution in the second extracting step is sulfuric acid/methanol solution, sulfuric acid/ethanol solution, anhydrous hydrochloride/methanol solution, anhydrous hydrochloride/ethanol solution, acetyl chloride/methanol, and acetyl chloride/ethanol solution. The acid/alcohol solution in the second extracting step has 1-5 % (w/v) of concentration. The acid/alcohol solution in the second extracting step is applied to 0.2-20 times of volume (v/w) to the crude lipid.
Abstract translation: 目的：提供生产生物柴油的方法，使用培养的动物细胞废物制造生物柴油，而不需要单独的废物处理过程。 构成：从培养的动物细胞回收制造生物柴油的方法包括向所收集的湿培养动物细胞回收中加入极性有机溶剂并搅拌以提取脂质的第一提取步骤; 粗制脂质产生步骤，分层上清液和细胞沉淀并浓缩上清液以制备浓缩物; 第二提取步骤，加入酸/醇溶液以产生粗脂质，用非极性有机溶剂回流和萃取; 以及收集非极性溶剂层并在减压下浓缩以分离生物柴油的分离步骤。 动物细胞是CHO（中国仓鼠卵巢）细胞，BHK（幼仓鼠肾）细胞，HEK（人胚胎肾）细胞和NS0（骨髓瘤细胞系）细胞）。 动物细胞是CHO细胞。 极性有机溶剂为甲醇，乙醇，异丙醇，丙酮或甲醇/氯仿溶剂混合物。 将极性有机溶剂以体积的1-10倍施加到培养的动物细胞补救的体积上。 甲醇/氯仿混合溶液的混合比为1:4至4:1。 第二提取步骤中的酸/醇溶液是硫酸/甲醇溶液，硫酸/乙醇溶液，无水盐酸盐/甲醇溶液，无水盐酸/乙醇溶液，乙酰氯/甲醇和乙酰氯/乙醇溶液。 第二提取步骤中的酸/醇溶液的浓度为1-5％（w / v）。 将第二提取步骤中的酸/醇溶液施加到粗脂质体积的0.2-20倍体积（v / w）。
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公开(公告)号：KR1020120014370A
公开(公告)日：2012-02-17
申请号：KR1020100076389
申请日：2010-08-09
Applicant: 인하대학교 산학협력단
Abstract: PURPOSE: A photobioreactor with a culture container and a wing is provided to shake the culture container and to mix a culture medium. CONSTITUTION: A photobioreactor(10) has a culture container(1) and wings(2) attached to the culture container. The wings mix a culture medium by shaking the culture container through the force of wind or water. The wings are extended to each opposite direction and cross each other. The culture container is made of a transparent material.
Abstract translation: 目的：提供具有培养容器和翼的光生物反应器以摇动培养容器并混合培养基。 构成：光生物反应器（10）具有附着在培养容器上的培养容器（1）和翼（2）。 翅膀通过风或水的力量摇动培养容器来混合培养基。 翼向每个相反方向延伸并交叉。 培养容器由透明材料制成。
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Patent Agency Ranking
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公开(公告)号：KR1020170112334A
公开(公告)日：2017-10-12
申请号：KR1020160039252
申请日：2016-03-31
Applicant: 인하대학교 산학협력단
Abstract: 본발명은바닥면및 측벽에의해배양대상광합성미생물을포함하는미세조류배양액과환경수를구획하고상부가개방된, 전부또는일부가불투광성소재로구성된배양용기; 및상기배양용기를고정함과동시에수면위로부양하기위한부양수단을포함하는, 미세조류수상배양을위한광생물반응기를제공한다.
Abstract translation: 本发明涉及一种包含微藻培养液的培养容器，所述微藻培养液含有由底面和侧壁培养的光合微生物并且其上部开放， 本发明还提供了用于微藻培养的光生物反应器。本发明还提供了用于微藻培养的光生物反应器。
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公开(公告)号：KR1020170098014A
公开(公告)日：2017-08-29
申请号：KR1020160019860
申请日：2016-02-19
Applicant: 인하대학교 산학협력단
Abstract: 본발명은배양대상내염성광합성미생물이포함된자연해수에염화나트륨또는천일염을첨가하거나상기자연해수를증발시켜고염도배지를제조하는고염도배지제조단계; 및상기광합성미생물을상기고염도배지에서배양하는고염도배지배양단계를포함하는, 오염방지및 지질생산성을증가시키는광합성미생물의배양방법을제공한다.
Abstract translation: 本发明是在高盐dobaeji制成通过加入氯化钠或太阳能盐，或天然海水中含有培养的靶耐盐光合微生物工序的天然海水蒸发产生高盐dobaeji; 以及在高盐石蜡中培养光合微生物的步骤，其中光合微生物在高盐寄生物中培养。
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公开(公告)号：KR1020150120843A
公开(公告)日：2015-10-28
申请号：KR1020140182993
申请日：2014-12-18
Applicant: 인하대학교 산학협력단
CPC classification number: C12N1/12 , C12M21/02 , C12M23/18 , C12M29/04 , C12M23/44 , C12M23/50 , Y02E50/13
Abstract: 본발명은 (a) 배양액은통과하되미세조류는통과하지않는배양용기를포함하는광생물반응기를환경수에침지하는단계; 및 (b) 상기배양용기의내부에환경수를추가적으로공급하는단계를포함하는것을특징으로하는미세조류의배양방법에관한것으로, 본발명을통해경제적이며효율적인미세조류의대량배양이가능할것으로기대된다.
Abstract translation: 本发明涉及一种培养微藻的方法，包括以下步骤：（a）将包含培养液通过但微藻不经过环境水的培养容器的光生物反应器浸渍; 和（b）另外向培养容器中供应环境水。 通过本发明预期微藻的经济和有效的大量培养。
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