公开(公告)号：KR100875437B1

公开(公告)日：2008-12-22

申请号：KR1020060001479

申请日：2006-01-05

Applicant: 재단법인서울대학교산학협력재단

Inventor： 강경선

IPC: C12N15/63 ,  A61K48/00

Abstract: 본 발명은
NPC1 유전자가 신경줄기세포의 자체재생 및 분화에 관여한다는 새로운 기능규명에 근거한 것으로, 신경줄기세포의 자체재생 및 분화에 관여하는
NPC1 유전자, 상기
NPC1
유전자를 함유하는 유전자 전달체를 유효성분으로 포함하는 퇴행성 신경계 질환, 심근경색 등과 같은 줄기세포 재생 또는 분화 불량에 의한 질환 치료용 유전자치료제 및 상기
NPC1
유전자를 함유하는 신경줄기세포를 유효성분으로 포함하는 퇴행성 신경계 질환, 심근경색 등과 같은 줄기세포 재생 또는 분화 불량에 의한 질환 치료용 세포치료제에 관한 것이다.
NPC1 유전자, 니이만 픽 병, 세포치료제

Abstract translation: 本发明

公开(公告)号：KR1020080101140A

公开(公告)日：2008-11-21

申请号：KR1020070047472

申请日：2007-05-16

Applicant: 주식회사 알바이오 ,  재단법인서울대학교산학협력재단

Inventor： 라정찬 ,  강경선 ,  신일섭 ,  서주연

IPC: A61K35/51 ,  A61K35/28

CPC classification number: A61K35/51 ,  A61K35/28

Abstract: A pharmaceutical composition is provided to replace damaged liver cells using mesenchymal stem cell, to recover function of the liver and to prevent and treat a liver fibrosis or a liver sclerosis by reducing collagen fiber deposited in the liver. A composition for preventing and treating a liver fibrosis or a liver sclerosis includes mesenchymal stem cell originated from an umbilical cord blood or a fatty tissue. The umbilical cord blood or the fatty tissue is separated from human. A pharmaceutical composition for preventing and treating the liver fibrosis or the liver sclerosis includes the mesenchymal stem cell originated from the umbilical cord blood or the fatty tissue. The umbilical cord blood or the fatty tissue is separated from human.

Abstract translation: 提供药物组合物以使用间充质干细胞代替损伤的肝细胞，通过减少沉积在肝脏中的胶原纤维来恢复肝脏的功能并预防和治疗肝纤维化或肝硬化。 用于预防和治疗肝纤维化或肝硬化的组合物包括起源于脐带血或脂肪组织的间充质干细胞。 脐带血或脂肪组织与人分离。 用于预防和治疗肝纤维化或肝硬化的药物组合物包括起源于脐带血或脂肪组织的间充质干细胞。 脐带血或脂肪组织与人分离。

公开(公告)号：KR100818216B1

公开(公告)日：2008-04-01

申请号：KR1020060099052

申请日：2006-10-11

Applicant: 재단법인서울대학교산학협력재단

Inventor： 강경선

IPC: C12N5/071 ,  C12N5/00

Abstract: A method for identifying an adult stem cell is provided to express Oct4, which is a marker to a multipotent stem cell, as well as Cripto-1, which is a marker for indifferentiation state, in the adult stem cell, thereby being useful for identifying more closely genuine adult stem cell. A method for identifying and isolating adult stem cells or tumor stem cells derived from human adipose tissue comprises the steps of: (a) immunostaining a group of adult stem cell or tumor stem cell candidates derived from the adipose tissue using an antibody binding to Cripto-1 and Oct4; (b) identifying the stem cell candidates group showing positive characteristic both on the Cripto-1 and Oct4 as the adult stem cells or tumor stem cells derived from the adipose tissue using a flow cytometer; and (c) isolating the identified stem cells.

Abstract translation: 提供了一种用于鉴定成体干细胞的方法，以在成体干细胞中表达作为多能干细胞的标记的Oct4以及作为分化状态的标记的Cripto-1，从而可用于鉴定 更真实的成体干细胞。 用于鉴定和分离源自人类脂肪组织的成体干细胞或肿瘤干细胞的方法包括以下步骤：（a）使用结合Cripto-1的抗体免疫一组源自脂肪组织的成体干细胞或肿瘤干细胞候选物， 1和Oct4; （b）使用流式细胞仪鉴定在Cripto-1和Oct4上显示阳性特征的干细胞候选组成为来源于脂肪组织的成体干细胞或肿瘤干细胞; 和（c）分离鉴定的干细胞。

公开(公告)号：KR100818215B1

公开(公告)日：2008-04-01

申请号：KR1020060099049

申请日：2006-10-11

Applicant: 재단법인서울대학교산학협력재단

Inventor： 강경선

IPC: C12N5/071 ,  C12N5/02

CPC classification number: C12N5/0636 ,  C12N5/0647 ,  C12N2501/125 ,  C12N2501/145 ,  C12N2501/26 ,  C12N2506/11

Abstract: A method for preparing a T-cell progenitor from a CD34 positive stem cell is provided to control the differentiation direction of a hematopoietic precursor in an STF(stem cell factor, Thrombopoietin, Flk-2/Flt3 ligand) medium, proliferate the T-cell progenitor in large scale and be useful for cell therapy relating to AIDS, cancer and auto-immune diseases. A method for preparing a T-cell progenitor from a CD34 positive stem cell comprises the steps of: (a) isolating CD34 positive stem cells derived from blood selected from the group consisting of human peripheral blood, bone marrow and cord blood; and (b) culturing and differentiating the isolated CD34 positive stem cells in a medium containing at least one cytokine selected from the group consisting of thrombopoietin(TPO), stem cell factor(SCF) and flk-2/flt3 ligand(FL) and not including thymic organ to obtain the T-cell progenitor showing negative immunological characteristic on CD2 and positive immunological characteristic on CD4, wherein the medium is an STF(stem cell factor, Thrombopoietin, Flk-2/Flt3 ligand) medium.

Abstract translation: 提供了一种从CD34阳性干细胞制备T细胞祖细胞的方法，以控制STF（干细胞因子，血小板生成素，Flk-2 / Flt3配体）培养基中造血前体的分化方向，增殖T细胞 大量的祖细胞，可用于与艾滋病，癌症和自身免疫疾病有关的细胞治疗。 从CD34阳性干细胞制备T细胞祖细胞的方法包括以下步骤：（a）分离源自选自人外周血，骨髓和脐带血的血液的CD34阳性干细胞; （b）在含有至少一种选自血小板生成素（TPO），干细胞因子（SCF）和flk-2 / flt3配体（FL）的细胞因子的培养基中培养和分化分离的CD34阳性干细胞，而不是 包括胸腺器官以获得在CD2上显示出阴性免疫学特征的CD4细胞祖细胞和CD4上的阳性免疫特征，其中培养基是STF（干细胞因子，血小板生成素，Flk-2 / Flt3配体）培养基。

公开(公告)号：KR100818214B1

公开(公告)日：2008-04-01

申请号：KR1020060095990

申请日：2006-09-29

Applicant: 재단법인서울대학교산학협력재단

Inventor： 강경선

IPC: A61K35/50 ,  C12N5/073

CPC classification number: A61K35/50 ,  C12N5/0605 ,  C12N2502/025

Abstract: A multipotent stem cell derived from decidua or amnion isolated from human term placenta is provided to express both Oct4 and Cripto-1, be differentiated into mesenchymal stem cells, epithelial stem cells and neurons, thereby being applicable as a cell therapeutic agent due to excellent proliferation rate and being useful as a cell therapeutic agent of brain diseases such as Alzheimer's disease, Parkinson's disease, brain and spinal cord injury, and stroke. A method for preparing an adult stem cell comprises a step of culturing a pellet derived from amnion or decidua of human term placenta in a medium containing NAC(N-acetyl-L-cysteine) and then recovering the culture material, wherein the adult stem cell shows positive immunological characteristic on both Oct4 and Cripto-1 and has the ability of being differentiated into cells derived from endoderm, mesoderm and ectoderm and the adult stem cell derived from the amnion shows positive immunological characteristics on CD44, CD49b, CD73, CD90, CD24 and CD29 and negative immunological characteristics on CD4, CD33, CD34, CD45, CD62p, CD133, CD135 and HLA-DR. Further, the medium containing NAC(N-acetyl-L-cysteine) is a Keratinocyte-SFM(Serum-Free Medium) containing additionally an FBS(Fetal Bovine Serum), an ascorbic acid, a calcium, an rEGF(epidermal growth factor), a BPE(1,2-Bis (4-Pyridyl)Ethane), an insulin and a hydrocortisone.

Abstract translation: 提供衍生自从人类胎盘分离的蜕膜或羊膜的多能干细胞以表达Oct4和Cripto-1，被分化为间充质干细胞，上皮干细胞和神经元，因此由于优异的增殖而适用于细胞治疗剂 并且可用作脑疾病如阿尔茨海默病，帕金森病，脑和脊髓损伤以及中风的细胞治疗剂。 用于制备成体干细胞的方法包括在含有NAC（N-乙酰基-L-半胱氨酸）的培养基中培养源自人类胎盘的羊膜或蜕膜的颗粒的步骤，然后回收培养物质，其中成体干细胞 在Oct4和Cripto-1上显示出阳性免疫学特征，并且具有分化为源自内胚层，中胚层和外胚层的细胞的能力，并且源自羊膜的成体干细胞在CD44，CD49b，CD73，CD90，CD24上显示出阳性免疫学特征 和CD4，CD33，CD34，CD45，CD62p，CD133，CD135和HLA-DR上的CD29和阴性免疫学特征。 此外，含有NAC（N-乙酰基-L-半胱氨酸）的培养基是另外含有FBS（胎牛血清），抗坏血酸，钙，rEGF（表皮生长因子）的角质细胞-SFM（无血清培养基） ，BPE（1,2-双（4-吡啶基）乙烷），胰岛素和氢化可的松。

公开(公告)号：KR100792184B1

公开(公告)日：2008-01-07

申请号：KR1020060042850

申请日：2006-05-12

Applicant: 재단법인서울대학교산학협력재단

Inventor： 강경선

IPC: C12N5/07 ,  C12N5/00

CPC classification number: A61K35/51 ,  A61K35/34 ,  A61K35/50 ,  C12N5/0607

Abstract: 본 발명은 개의 제대혈 및 개 태아의 심장 유래 다분화능 성체 줄기세포, 그 제조방법 및 이를 함유하는 세포 치료제에 관한 것으로, 더욱 자세하게는, 개의 제대혈 및 개 태아의 심장 유래 혈액에서 분리한 유핵세포를 FBS를 함유하는 배지에 배양하여 분리한 다분화능 성체 줄기세포, 그 제조방법 및 이를 함유하는 세포 치료제에 관한 것이다.
본 발명에 따른 성체 줄기세포는 개의 제대혈, 태반 및 개 태아의 심장에서 유래한 것으로서, 사람의 간엽세포 성격과 매우 유사한 성격을 가지고 있으면서도, 사람 제대혈 유래 간엽세포보다 초기 단계에 왕성한 세포 성장을 하므로 개의 난치병, 불치병 치료에 유용하게 사용될 수 있고, 골형성 세포 및 신경세포로 분화하는 능력을 가지고 있어, 근골격계 질환 및 뇌 신경계 질병 등의 치료에 유용하다.
제대혈, 태반, 성체 줄기세포, 다분화능

公开(公告)号：KR1020070109615A

公开(公告)日：2007-11-15

申请号：KR1020060042850

申请日：2006-05-12

Applicant: 재단법인서울대학교산학협력재단

Inventor： 강경선

IPC: C12N5/07 ,  C12N5/00

CPC classification number: A61K35/51 ,  A61K35/34 ,  A61K35/50 ,  C12N5/0607

Abstract: An adult stem cell derived from canine cord blood, placenta and canine fetus heart is provided to be usefully used for treating incurable diseases of canine and be effective for treating musculoskeletal system disorders or neurological disorders, wherein the adult stem cell shows similar characteristic to mesenchymal cells of human, vigorously grows in an early stage and is differentiated into osteogenic cells and neurons. A method for preparing an adult stem cell comprises a step of culturing nucleated cells isolated from canine cord blood, placenta and canine fetus heart in DMEM containing 1-30% of FBS, wherein the adult stem cell shows at least one positive immunological characteristic regarding MHC CL, Thy.1 and CD44(BD) and negative immunological characteristic regarding CD34 at the same time, is attached to plastic to grow, has morphological characteristics of spindle-shape and is able to be differentiated into cells derived from endoderm, ectoderm, and mesoderm. A cell therapeutic agent for treating musculoskeletal system disorders or neurological disorders comprises the adult stem cell as an effective ingredient.

Abstract translation: 提供衍生自犬脐带血，胎盘和犬胎儿心脏的成体干细胞用于治疗犬的不治之症，并且对于治疗肌肉骨骼系统疾病或神经障碍有效，其中成体干细胞显示与间充质细胞相似的特征 的人类，在早期大量生长，并分化成成骨细胞和神经元。 一种用于制备成体干细胞的方法包括在含有1-30％FBS的DMEM中培养从犬脐带血，胎盘和犬胎儿心脏分离的有核细胞的步骤，其中成体干细胞显示至少一种关于MHC的阳性免疫学特征 CL，Thy.1和CD44（BD）和CD34的免疫学特性同时与塑料附着生长，具有纺锤形状的形态特征，能够分化成源自内胚层，外胚层和 中胚层。 用于治疗肌肉骨骼系统疾病或神经障碍的细胞治疗剂包括成体干细胞作为有效成分。

公开(公告)号：KR1020070011417A

公开(公告)日：2007-01-24

申请号：KR1020067022611

申请日：2004-04-13

Applicant: (주)지노첵 ,  재단법인서울대학교산학협력재단 ,  주식회사 이즈텍 ,  (주)신원사이언스

Inventor： 황승용 ,  정진욱 ,  오문주 ,  김승준 ,  연종필 ,  김준섭 ,  김용현 ,  이창현 ,  윤현규 ,  염혜정 ,  강경선 ,  이영순 ,  박준석 ,  황재웅 ,  김양석 ,  이완선 ,  전기선 ,  엄찬휘 ,  강종수 ,  이경재 ,  송복수 ,  전금진 ,  여찬동

IPC: C12Q1/68

CPC classification number: C12Q1/6883 ,  C12Q2600/158 ,  C12Q1/6837

Abstract: A DNA chip and a kit for screening substances showing activity as a peroxisome proliferator are provided to be able to rapidly and accurately screen the peroxisome proliferator inducing abnormal cell reaction in vivo, thereby being widely applied to safety test of various materials. The DNA chip for screening of peroxisome proliferator comprises: a probe consisting of 121 to 587 nucleotides, capable of binding specifically to a gene whose expression is induced by a peroxisome proliferators; a linker including oligo (dT)15, -(CH2)6- and amine group in sequence, whose 3'-terminal of oligo (dT)15 is linked to 5'-terminal of the probe; and a solid substrate, whose surface is coated with aldehyde group, connecting with amine group of the linker via Schiff's base reaction. In the DNA chip, the probe is at least one gene selected from SEQ ID Nos: 1 to 12. The kit for screening of peroxisome proliferator comprises the DNA chip and a fluorescence-labeled deoxynucleotide to be used for labeling cDNA synthesized from RNA isolated from a sample. The method for screening peroxisome proliferator using the kit comprises the steps of: (a) administering a peroxisome proliferator candidate chemical to an animal; (b) isolating RNA from the animal; (c) synthesizing fluorescence-labeled cDNA from the isolated RNA using fluorescence-labeled deoxynucleotide in the kit; (d) applying the synthesized cDNA to a DNA chip for screening peroxisome proliferator in the kit to allow hybridization; and (e) after washing the hybridized DNA chip, examining the hybridized probe in the DNA chip by using a scanner.

Abstract translation: 提供了用于筛选显示出作为过氧化物酶体增殖物活性的物质的DNA芯片和试剂盒，以能够快速且准确地筛选在体内诱导异常细胞反应的过氧化物酶体增殖物，从而广泛应用于各种材料的安全性试验。 用于筛选过氧化物酶体增殖物的DNA芯片包括：由121至587个核苷酸组成的探针，能够特异性结合其表达由过氧化物酶体增殖物诱导的基因; 包含寡核苷酸（dT）15， - （CH2）6-和胺基的接头，其寡核苷酸（dT）15的3'-末端连接到探针的5'-末端; 和表面用醛基涂覆的固体基质，通过Schiff碱反应与连接体的胺基连接。 在DNA芯片中，探针是选自SEQ ID NO：1〜12的至少一种基因。用于筛选过氧化物酶体增殖物的试剂盒包含DNA芯片和荧光标记的脱氧核苷酸，用于标记从分离自 一个样品。 使用试剂盒筛选过氧化物酶体增殖物的方法包括以下步骤：（a）向动物施用过氧化物酶体增殖物候选化学品; （b）从动物中分离RNA; （c）使用试剂盒中荧光标记的脱氧核苷酸从分离的RNA合成荧光标记的cDNA; （d）将合成的cDNA应用于DNA芯片，用于筛选试剂盒中的过氧化物酶体增殖物以进行杂交; 和（e）在洗涤杂交的DNA芯片之后，通过使用扫描仪检查DNA芯片中的杂交探针。

公开(公告)号：KR1020060093269A

公开(公告)日：2006-08-24

申请号：KR1020060001479

申请日：2006-01-05

Applicant: 재단법인서울대학교산학협력재단

Inventor： 강경선

IPC: C12N15/63 ,  A61K48/00

Abstract: 본 발명은
NPC1 유전자가 신경줄기세포의 자체재생 및 분화에 관여한다는 새로운 기능규명에 근거한 것으로, 신경줄기세포의 자체재생 및 분화에 관여하는
NPC1 유전자, 상기
NPC1
유전자를 함유하는 유전자 전달체를 유효성분으로 포함하는 퇴행성 신경계 질환, 심근경색 등과 같은 줄기세포 재생 또는 분화 불량에 의한 질환 치료용 유전자치료제 및 상기
NPC1
유전자를 함유하는 신경줄기세포를 유효성분으로 포함하는 퇴행성 신경계 질환, 심근경색 등과 같은 줄기세포 재생 또는 분화 불량에 의한 질환 치료용 세포치료제에 관한 것이다.
NPC1 유전자, 니이만 픽 병, 세포치료제

公开(公告)号：KR1020080048268A

公开(公告)日：2008-06-02

申请号：KR1020060118443

申请日：2006-11-28

Applicant: 재단법인서울대학교산학협력재단

Inventor： 강경선

IPC: G01N33/574 ,  G01N33/53

Abstract: A method for screening an anti-cancer material is provided to screen simply the anti-cancer material based on the finding of a human breast tumor stem cell not-expressing connexin 43, expressing Oct-4, an estrogen receptor and Cripto-1 and increasing the expression of estrogen, thereby screening a chemo-preventive agent and a therapeutic agent very rapidly at low cost without using a rodent model or an in vitro model and being usefully used for studying mechanism of a human breast cancer carcinogenesis. A method for screening an anti-cancer material comprises the steps of: (a) culturing an adult stem cell or a tumor stem cell derived from a human tissue to prepare a stem cell sphere; (b) treating the sphere with an anti-cancer candidate material; (c) identifying a stem cell marker such as Oct-4, Cripto-1, ABCG2, an estrogen receptor and connexin using an antibody or a gene coupled to the stem cell marker; and (d) selecting a material where the expression of the Oct-4, Cripto-1, ABCG2, and estrogen receptor is decreased or the expression of the connexin is increased, compared with a case of not-being treated with an anti-cancer candidate material as an anti-cancer material, wherein the human tissue derived adult stem cell is an adult stem cell derived from the human tissue selected from the group consisting of breast, bone marrow, umbilical cord blood, blood, liver, skin, gastrointestinal tract, placenta and uterus and the tumor stem cell is a tumor stem cell derived from a tumor selected from the group consisting of breast cancer, brain tumor, gastrointestinal tract cancer, lung cancer, liver cancer, uterine carcinoma, large intestine cancer and myelogenous leukemia.

Abstract translation: 提供筛选抗癌材料的方法，以基于发现不表达Oct-4的人乳腺肿瘤干细胞，表达Oct-4，雌激素受体和Cripto-1的增加的抗癌材料 雌激素的表达，从而以低成本非常快速地筛选化学预防剂和治疗剂，而不使用啮齿动物模型或体外模型，并且有效地用于研究人乳腺癌致癌机制。 筛选抗癌材料的方法包括以下步骤：（a）培养来自人体组织的成体干细胞或肿瘤干细胞以制备干细胞球; （b）用抗癌候选物质处理球体; （c）使用抗体或与干细胞标记物偶联的基因鉴定干细胞标记如Oct-4，Cripto-1，ABCG2，雌激素受体和连接蛋白; 和（d）与未用抗癌治疗的情况相比，选择Oct-4，Cripto-1，ABCG2和雌激素受体的表达降低或连接蛋白的表达增加的材料 候选材料作为抗癌材料，其中所述人体组织来源的成体干细胞是源于选自乳腺，骨髓，脐带血，血液，肝脏，皮肤，胃肠道的人体组织的成体干细胞 胎盘和子宫，肿瘤干细胞是衍生自选自乳腺癌，脑肿瘤，胃肠道癌，肺癌，肝癌，子宫癌，大肠癌和骨髓性白血病的肿瘤的肿瘤干细胞。