公开(公告)号：KR1020030034420A

公开(公告)日：2003-05-09

申请号：KR1020010065351

申请日：2001-10-23

Applicant: 주식회사 엘컴사이언스 ,  주식회사머쉬텍

Inventor： 김영중 ,  성재모 ,  성상현 ,  이미경 ,  김승현

IPC: A61K36/07 ,  A61P25/28

Abstract: PURPOSE: A pharmaceutical composition containing, as an active ingredient, an extract of mycelium of Phellinus linteus and a fraction thereof is provided. The composition has nerve cell protecting activity and has no toxicity and is thus effectively used as a therapeutic or treating agent for neurodegenerative diseases such as cerebral apoplexy, dementia, etc. CONSTITUTION: The pharmaceutical composition contains a methanol extract of mycelium of Phellinus linteus, or an EtOAc fraction or a n-hexane fraction thereof having nerve cell protecting activity. It is formulated into a pharmaceutically acceptable formulation with a pharmaceutically acceptable excipient. For an example, 5kg of mycelium of Phellinus linteus is added with 4ml MeOH, followed by sonication, filtration and concentration under reduced pressure to produce 365g of an extract. Thereafter, the extract is suspended in distilled water, followed by fractionation with n-hexane, EtOAc, n-BuOH and concentration under reduced pressure to produce a n-hexane fraction(49g), an EtOAc fraction(34g), a n-BuOH fraction(100g) and a H2O fraction(182g).

Abstract translation: 目的：提供含有桑黄菌丝体提取物及其一部分作为活性成分的药物组合物。 该组合物具有神经细胞保护活性并且没有毒性，因此有效地用作脑中风，痴呆等神经变性疾病的治疗或治疗剂。构成：药物组合物含有桑黄菌丝体的甲醇提取物，或 具有神经细胞保护活性的EtOAc级分或其正己烷级分。 将其与药学上可接受的赋形剂配制成药学上可接受的制剂。 例如，将5kg的桑黄菌丝体加入4ml MeOH，然后超声处理，过滤并减压浓缩，得到365g提取物。 然后将提取物悬浮在蒸馏水中，然后用正己烷，EtOAc，n-BuOH分级并减压浓缩，得到正己烷部分（49g），EtOAc级分（34g），n-BuOH 级分（100g）和H 2 O级分（182g）。

公开(公告)号：KR1020030008247A

公开(公告)日：2003-01-25

申请号：KR1020010042944

申请日：2001-07-16

Applicant: 주식회사머쉬텍 ,  성재모

Inventor： 성재모 ,  김태웅 ,  김호경

IPC: A23F3/00

Abstract: PURPOSE: A process for preparing heath tea containing Cordyceps militaris by freeze-drying mycelia and fruit bodies of Cordyceps militaris and grinding them is provided. Whereby, the tea product is excellent in its taste and flavor and color. CONSTITUTION: Liquid mushroom spawn of Cordyceps militaris is inoculated into and cultured in a medium obtained by sterilizing unpolished rice and pupa at 121deg.C for 30min under high pressure to produce mycelia and fruit bodies of Cordyceps militaris, which are freeze-dried, ground and mixed with one of a medium composition for cultivating Paecilomyces Japonica, Cordyceps bassiana, Cordyceps pruinosa and Phellinus linteus.

Abstract translation: 目的：通过冷冻干燥冬虫夏草的菌丝体和果实来研磨含有冬虫夏草的健康茶，并进行研磨。 因此，茶产品的味道和风味和颜色优良。 构成：将冬虫夏草的液体蘑菇产卵在121℃高温灭菌30分钟，接种培养基中培养30分钟，制成冬虫夏草的菌丝体和果实，将其冻干，粉碎， 与培养拟青霉，冬虫夏草，冬虫夏草和番茄的培养基组合物混合。

公开(公告)号：KR100362901B1

公开(公告)日：2002-12-11

申请号：KR1020000035752

申请日：2000-06-27

Applicant: 주식회사머쉬텍 ,  성재모

Inventor： 성재모

IPC: A01G1/04

Abstract: 본 발명은 풍뎅이동충하초 자실체의 대량생산방법에 관한 것으로, 본 발명은 풍뎅이동충하초 균주를 탄소원, 질소원 및 물을 함유하는 배양액에서 배양하여 잡균의 오염없이 활력이 우수한 액체종균을 제조하는 뛰어난 효과가 있고 상기 제조한 액체종균을 곡립과 누에번데기 가루를 함유하는 배지에서 배지의 표면에 자실체가 완전히 덮히도록 생육시켜 풍뎅이동충하초를 대량으로 생산하는 뛰어난 효과가 있다.

公开(公告)号：KR100362900B1

公开(公告)日：2002-12-11

申请号：KR1020000035751

申请日：2000-06-27

Applicant: 주식회사머쉬텍 ,  성재모

Inventor： 성재모

IPC: A01G1/04

Abstract: 본 발명은 붉은자루동충하초 자실체의 대량생산방법에 관한 것으로, 본 발명은 붉은자루동충하초 균주를 탄소원, 질소원 및 물을 함유하는 배양액에서 배양하여 잡균의 오염없이 활력이 우수한 액체종균을 제조하는 뛰어난 효과가 있고 상기 제조한 액체종균을 곡립과 누에번데기 작은 조각을 함유하는 배지에서 배지의 표면에 자실체가 완전히 덮히도록 생육시켜 붉은자루동충하초를 대량으로 생산하는 뛰어난 효과가 있다.

公开(公告)号：KR1020020014227A

公开(公告)日：2002-02-25

申请号：KR1020000047398

申请日：2000-08-17

Applicant: 주식회사머쉬텍 ,  성재모

Inventor： 성재모

IPC: A01G1/04

CPC classification number: C12N1/14

Abstract: PURPOSE: Provided are a method and an apparatus for seed culture of Cordyceps sp. strains using a liquid medium consisting of dextrose, yeast extract, peptone and sterilized solution for the mass production of its fruit body. CONSTITUTION: The fruit body of Cordyceps sp. strains is mass-produced by culturing Cordyceps sp. strains in a solid medium consisting of 10-80gm of dextrin, 2-20gm yeast extract, 2-2gm of peptone, 10-20gm of agar and 1000mL of distilled water in an incubator of 22-30 deg.C for 4-8 days; manufacturing an inoculant medium consisting of 10-80gm of dextrin, 2-20gm of yeast extract, 2-20gm of peptone and 1000mL of distilled water; inserting the inoculant medium into an inoculation apparatus; inoculating the cultured Cordyceps sp. strains thereto then culturing inoculants at 22-30 deg.C for 4-8days; manufacturing a liquid medium for the seed culture consisting of 10-80gm of dextrose, 2-20gm of yeast extract, 2-20gm of peptone, 0.1-1.0%(w/v) of endoplasmic reticulum and 1000mL of distilled water; inoculating the cultured inoculants to the liquid medium then culturing the medium at 22-28 deg.C for 5-12 days with flowing sterilized air with 0.1-1.0 vvm; inoculating the cultured seeds to a medium, which is manufactured by mixing and sterilizing brown rice and a cocoon in a 1000mL semi transparent plastic container, then culturing them for 10-15 days; then culturing them for 15-18 days more with maintaining the temperature of 20 deg.C, the humidity of 80-90%, the brightness of 500-1000 Lux.

Abstract translation: 目的：提供冬虫夏草种子培养的方法和装置。 菌株使用由葡萄糖，酵母提取物，蛋白胨和灭菌溶液组成的液体培养基，用于大量生产其果实体。 构成：冬虫夏草的果实体 菌株通过培养冬虫夏草大量生产。 菌株在由10-80g糊精，2-20gm酵母提取物，2-2gm蛋白胨，10-20gm琼脂和1000mL蒸馏水组成的固体培养基中，在22-30℃的培养箱中培养4-8天 ; 制造由10-80g糊精，2-20gm酵母提取物，2-20gm蛋白胨和1000mL蒸馏水组成的孕育剂培养基; 将接种培养基插入接种装置中; 接种培养的冬虫夏草 然后在22-30℃培养接种物4-8天; 制造用于种子培养的液体培养基，其由10-80g葡萄糖，2-20gm酵母提取物，2-20gm蛋白胨，0.1-1.0％（w / v）内质网和1000mL蒸馏水组成; 将培养的接种物接种到液体培养基中，然后在22-28℃培养培养基5-12天，用0.1-1.0vvm的流动灭菌空气; 将培养的种子接种到通过在1000mL半透明塑料容器中混合灭菌糙米和茧制成的培养基，然后培养10-15天; 然后再培养15-18天，保持温度20℃，湿度80-90％，亮度500-1000Lux。

公开(公告)号：KR3002869160000S

公开(公告)日：2001-12-06

申请号：KR3020010011368

申请日：2001-04-30

Applicant: 주식회사머쉬텍 ,  성재모

Designer： 성재모

公开(公告)号：KR100266082B1

公开(公告)日：2000-10-02

申请号：KR1019980014788

申请日：1998-04-24

Applicant: 원종광 ,  성재모 ,  주식회사머쉬텍

Inventor： 성재모

IPC: A01G1/04

Abstract: PURPOSE: A method for cultivating a Cordyceps fruit body is provided to select for massively cultivating excellent fungi from Cordyceps fruit bodies at low costs, to prevent the invasion of various bacteria and to cultivate liquid-typed fungi. CONSTITUTION: Cordyceps fungi are cultured in a flat plate and expanded for preparing liquid-typed fungi. A grain culture medium is mixed with pupas in a ratio of 16:1, and put in a culture medium cultivating container with water for sterilizing the culture medium at 121deg.C for 30 minutes. The liquid-typed fungi are inoculated on the surface of the culture medium evenly. The temperature is kept at 24deg.C in having taken root of the fungi, and at 20deg.C in forming fruit bodies.

Abstract translation: 目的：提供一种栽培冬虫夏草水果体的方法，以低成本大量培养冬虫夏草果实的优良真菌，防止各种细菌的侵入和培养液体真菌。 构成：冬虫夏草真菌在平板上培养，并扩展用于制备液体真菌。 将颗粒培养基与蛹混合16:1，并在培养基培养容器中加入水，121℃灭菌培养基30分钟。 将液体型真菌均匀地接种于培养基表面。 在真菌根部保持24℃，在20℃下形成果实。

公开(公告)号：KR1019990085245A

公开(公告)日：1999-12-06

申请号：KR1019980017527

申请日：1998-05-15

Applicant: 주식회사머쉬텍 ,  원종광 ,  성재모

Inventor： 성재모

IPC: A01G1/04 ,  C12N1/14

Abstract: 본 발명은 보리를 필수성분으로 하여 상황버섯 균이 배지의 밑 부분까지 완전히 배양될 수 있는 배지를 조성하고, 이런 배지에서 배양된 상황버섯 균사체를 일정한 모양으로 성형하여 상품화할 수 있도록 하는 상황버섯 재배방법을 제공하는데 그 목적이 있다.
본 발명에 따르면, 상황버섯 균사체의 원균을 평판 배양시키고 확대 배양시켜 상황버섯종균을 준비하는 단계와; 상황버섯 균사체 배양배지용 곡류를 물과 함께 배지배양용기에 넣고, 이를 고온고압 살균솥에 넣어 멸균시켜서 상황버섯 균사체의 배양배지를 준비하는 단계 및; 상기 배양배지에 상기 종균을 접종시키는 단계를 거쳐 배양 완료된 상황버섯 균사체가 함유된 배지를 1∼2cm의 두께로 절단하여 2차 배양하거나 0.5∼1cm의 크기로 분쇄한 후 성형틀에 넣고 2차 배양하는 것을 특징으로 하는 상황버섯 재배방법이 제공된다.