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公开(公告)号：KR1020160077646A
公开(公告)日：2016-07-04
申请号：KR1020140187792
申请日：2014-12-24
Applicant: 한국에너지기술연구원
Abstract: 본발명은재생형식물전지에관한것으로서, 더욱상세하게는태양광발전이가능할때는태양광을이용하여전기를생산하고, 태양광을이용하여전기를생산함과동시에생산된수소와산소를저장하며, 태양광이충분하지못하거나야간에는저장된수소/산소를연료전지에공급하여전기를생산할수 있는재생형식물전지를제공한다.
Abstract translation: 本发明涉及一种再生植物细胞，更具体地说，涉及一种被配置为通过使用太阳光发电的再生植物细胞，当可以进行光伏发电时，存储同时由光伏发电产生的氧和氢， 将氧气和氢气储存在燃料电池中，夜间和阳光不足的时候，以产生电力。 根据本发明，再生植物细胞包括：光生电化学电池（PBEC）; 氢气储存单元; 氧气存储单元; 燃料电池单元; 储水单元; 和控制单元。
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公开(公告)号：KR1020150067458A
公开(公告)日：2015-06-18
申请号：KR1020130152832
申请日：2013-12-10
Applicant: 한국에너지기술연구원
CPC classification number: Y02E50/343 , Y02W10/37 , C12P5/02
Abstract: 본원은온도, 단계별기질주입, 미량금속첨가및 SBR 운전방식의조절을통한메탄생성방법을개시한다. 본원에따른방법은희석하지않은음식물쓰레기와같은높은유기물함량의기질을사용한운전이가능하여, 유기성폐기물로부터메탄을효과적으로바이오메탄을생산할수 있다.
Abstract translation: 本文公开了通过每温度或每级注入基材，添加痕量金属和控制SBR操作方法来生产甲烷的方法。 根据本发明的方法可以通过使用具有高有机含量的底物，例如未稀释的食物废物来进行，从而有效地从有机废物产生生物甲烷。
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公开(公告)号：KR101299115B1
公开(公告)日：2013-08-21
申请号：KR1020130029728
申请日：2013-03-20
Applicant: 한국에너지기술연구원
Abstract: PURPOSE: A high speed production method of hydrogen using a granulated thermophilic strain mixture is provided to enlarge a stirring effect through inner circulation and to constantly maintain a pH value in a reactor, thereby continuously reducing hydraulic retention time (HRT) and to maximize hydrogen production speed. CONSTITUTION: A high speed production method of hydrogen using a granulated thermophilic strain mixture comprises the steps of: heating sludge of a digester, sewage sludge, or soil at high temperature and preparing a strain mixture including hydrogen-producing bacteria; culturing the strain mixture in a continuous stirred tank reactor (CSTR); adding the mixture strain into an expanded granular sludge bed digestion (EGSB) and granulating the strain mixture; adding a substrate to the EGSB; adding nutrients and a buffer solution to the EGSB; and performing fermentation in the EGSB and obtaining hydrogen. The nutrients increase the size of granules of the strain mixture. The buffer solution maintains the EGSB at pH 5-6.
Abstract translation: 目的：提供使用粒状嗜热菌应变混合物的高速生产氢气的方法，以通过内部循环扩大搅拌效果，并在反应器中不断保持pH值，从而持续降低水力停留时间（HRT）并最大化氢气产生 速度。 构成：使用造粒的嗜热菌株混合物的高速生产氢的方法包括以下步骤：在高温下加热蒸煮器，污水污泥或土壤的污泥，并制备包括产氢菌的菌株混合物; 在连续搅拌釜反应器（CSTR）中培养菌株混合物; 将混合菌株加入到膨胀的颗粒污泥床消化（EGSB）中并将菌株混合物制粒; 向EGSB添加底物; 向EGSB添加营养物质和缓冲溶液; 并在EGSB中进行发酵并获得氢。 营养物质增加了菌株混合物的颗粒大小。 缓冲溶液将EGSB保持在pH 5-6。
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公开(公告)号：KR101271005B1
公开(公告)日：2013-06-04
申请号：KR1020120059602
申请日：2012-06-04
Applicant: 한국에너지기술연구원
Abstract: PURPOSE: A method for producing lactic acid using a continuous anaerobic reactor containing granular lactic acid bacteria sludge is provided to reduce the volume of a reaction tank by short HRT(Hydraulic Residence Time), to enhance a production rate, and to continuously produce the lactic acid without additional culture or change of microorganisms. CONSTITUTION: A method for continuously producing lactic acid comprises: a step of culturing anaerobic digester sludge in a reactor and obtaining lactic acid bacteria containing Bacillus sp. and Lactobacillus sp.; a step of adding the lactic acid bacteria to the continuous anaerobic digester sludge and granulating; a step of adding a substrate to the continuous anaerobic digester sludge; and a step of fermenting and obtaining lactic acid. The lactic acid is obtained at 50-60 deg. C and at pH 4.5-5.
Abstract translation: 目的：提供一种使用含有颗粒状乳酸菌污泥的连续厌氧反应器生产乳酸的方法，通过短的HRT（液压停留时间）来减少反应罐的体积，提高生产率，并连续生产乳酸 酸，无需额外的培养或微生物的变化。 构成：连续生产乳酸的方法包括：在反应器中培养厌氧消化池污泥并获得含有芽孢杆菌属的乳酸菌的步骤。 和乳杆菌属。 向连续厌氧消化池污泥中加入乳酸菌并制粒的步骤; 向连续厌氧消化池污泥添加底物的步骤; 以及发酵和获得乳酸的步骤。 乳酸在50-60℃得到。 并在pH 4.5-5。
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公开(公告)号：KR1020120126237A
公开(公告)日：2012-11-21
申请号：KR1020110043884
申请日：2011-05-11
Applicant: 한국에너지기술연구원
Abstract: PURPOSE: A method for biologically preparing hydrogen using organic waste is provided to treat food waste in an eco-friendly method. CONSTITUTION: A method for biologically preparing hydrogen using organic waste comprises: a step of pulverizing the organic waste; a step of fermenting the waste under an anaerobic dark fermentation condition to prepare a first fermentation containing lactic acid; a step of isolating the first fermentation into a solid phase and a liquid phase; a step of putting the liquid phase into a photoreactor; a step of seeding hydrogen-producing bacteria to the liquid phase materials; and a step of fermenting the liquid phase materials under an anaerobic light fermentation condition. The organic waste is food waste. The hydrogen producing bacteria are Rhodopseudomonas sphaeroides KD131. [Reference numerals] (AA) Food waste 20-50 g carbo.COD/L pH=5.5; (BB) Lactic acid fermentation(glucose conversion efficiency: 80%); (CC) Photosynthesis fermentation(Rhodobacter sphaeroides KD131)
Abstract translation: 目的：提供一种利用有机废物生物制氢的方法，以环保方式处理食物废物。 构成：使用有机废物生物制氢的方法包括：粉碎有机废物的步骤; 在厌氧黑发酵条件下发酵废物以制备含有乳酸的第一发酵的步骤; 将第一发酵分离成固相和液相的步骤; 将液相放入光反应器的步骤; 将产氢细菌接种到液相材料的步骤; 以及在厌氧发光条件下发酵液相材料的步骤。 有机废物是食物废物。 产氢菌是红假单胞菌（Rhodopseudomonas sphaeroides）KD131。 （标号）（AA）食品废弃物20-50g carbo.COD / L pH = 5.5; （BB）乳酸发酵（葡萄糖转化效率：80％）; （CC）光合作用发酵（Rhodobacter sphaeroides KD131）
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Patent Agency Ranking
公开(公告)号：KR1020120086182A
公开(公告)日：2012-08-02
申请号：KR1020110007482
申请日：2011-01-25
Applicant: 한국에너지기술연구원
Abstract: PURPOSE: A method for producing hydrogen using condition-optimized Rhodobacter sphaeroides KD131 is provided to maximize hydrogen productivity. CONSTITUTION: A method for producing hydrogen using Rhodobacter sphaeroides KD131 comprises a step of culturing Rhodobacter sphaeroides KD131 using succinate as a carbon source and (NH_2)_4SO_4 or glutamate as a nitrogen source. The initial concentration and pH of the bacteria is 0.45 g dcw/l-0.67 g dcw/l and pH 6-7.
Abstract translation: 目的：提供使用条件优化的球形红细菌KD131来生产氢的方法，以最大化氢生产率。 构成：使用球形红细菌KD131生产氢的方法包括使用琥珀酸盐作为碳源和（NH 2）4 SO 4或谷氨酸作为氮源培养球状红细菌KD131的步骤。 细菌的初始浓度和pH为0.45g dcw / l-0.67g dcw / l和pH6-7。
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公开(公告)号：KR101007000B1
公开(公告)日：2011-01-12
申请号：KR1020080064176
申请日：2008-07-03
Applicant: 한국에너지기술연구원
Abstract: 본 발명은, 막의 오염을 최소화할 수 있도록 막모듈의 구성을 제안하고, 생물배양기의 혐기 발효조에서 유입된 배양액 유입관로와 혐기성 수소생성균을 포함하는 보류액 환류관로를 통해 연결되어진 외부스트림 흡입형 분리막 모듈이 부착된 구성을 제안하여 발효기 내 혐기 미생물 농축을 위한 막 모듈이 적용된 최적 수소생산 생물배양장치에 관한 것이다.
이를 위해 본 발명은, 수소를 발생시키기 위한 혐기세균이 저장된 혐기 발효조와; 상기 혐기세균이 수소를 생산하도록 하기 위한 기질을 혐기 발효조로 공급하는 기질 공급조와; 상기 기질 공급조에서 공급된 기질에 혐기세균이 결합하여 발생된 수소를 전달 받아 이를 포집하는 수소가스 포집장치와; 상기 수소가스 포집장치를 통해 혐기세균의 발효액을 전달받아 이를 농축하며 농축과정을 제어하기 위한 외부스트림 흡입형 막 모듈로 적용된 분리막 모듈과; 상기 분리막 모듈을 통과한 투과액을 전달받아 이를 여과하여 저수하는 투과액 수집조와; 상기 투과액 수집조에서 여과된 투과액을 다시 분리막 모듈로 공급하여 분리막 오염물질을 세척하는 역세공정라인;을 포함하여 구성된다.
혐기발효, 생물배양기, 농축막공정, 수소생산, 흡입형 분리막모듈-
48.发明公开
公开(公告)号：KR1020100004153A
公开(公告)日：2010-01-13
申请号：KR1020080064177
申请日：2008-07-03
Applicant: 한국에너지기술연구원
CPC classification number: C12P3/00
Abstract: PURPOSE: A Rhodobacter sphaeroides-derived mutant strain which generates hydrogen even without light is provided. CONSTITUTION: A photosynthetic bacteria mutant strain which is able to generate hydrogen is produced by adding pyrubic acid lyase and formic acid lyase to Rhodobacter sphaeroides(KCTC 12085). A complex of the pyruvic acid lyase and formic acid lyase is isolated from Rhodospirillum rubrum.
Abstract translation: 目的：提供即使没有光也产生氢的罗红假单胞菌衍生的突变菌株。 构成：通过向球形红细菌（KCTC 12085）中加入吡哆酸裂解酶和甲酸裂解酶来产生能够产生氢的光合细菌突变菌株。 丙酮酸裂解酶和甲酸裂合酶的复合物从红皮红丝菌中分离出来。
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公开(公告)号：KR1020080110165A
公开(公告)日：2008-12-18
申请号：KR1020070058562
申请日：2007-06-14
Applicant: 한국에너지기술연구원
CPC classification number: Y02E60/527 , H01M8/16 , H01M4/9016 , H01M4/96
Abstract: A microbial biofuel cell is provided to improve the battery capacity by forming nanowires (or nanorods) having high conductivity to a negative electrode. A microbial biofuel cell comprises a negative electrode(110) formed with a plurality of nanowires(112) delivering the electrons reduced by the reducing power generated by the energy metabolism of microorganism; a positive electrode(120) formed with a transition metal(122) capable of being oxidized with the oxygen; a reaction bath(130) to contact one side of the positive electrode with the air; an electrolyte(140) accommodated inside the reaction bath; and a microbial catalyst(150) accommodated inside the reaction bath.
Abstract translation: 提供微生物生物燃料电池以通过形成对负极具有高导电性的纳米线（或纳米棒）来改善电池容量。 微生物生物燃料电池包括形成有多个纳米线（112）的负极（110），所述多个纳米线（112）递送由微生物的能量代谢产生的还原能量减少的电子; 形成有能够被氧氧化的过渡金属（122）的正电极（120） 将正极的一侧与空气接触的反应槽（130） 容纳在反应槽内的电解质（140）; 和容纳在反应槽内的微生物催化剂（150）。
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公开(公告)号：KR100794384B1
公开(公告)日：2008-01-15
申请号：KR1020060073104
申请日：2006-08-02
Applicant: 한국에너지기술연구원
IPC: C12N9/02
Abstract: A method for producing and purifying hydrogenase is provided to produce the highly concentrated hydrogenase by culturing Thiocapsa roseopersicina in an acetate-added culture medium and purify the hydrogenase with stable activity from a culture material of the Thiocapsa roseopersicina. A method for producing and purifying hydrogenase comprises the steps of: (a) treating fungi bodies obtained by centrifuging a culture solution obtained by culturing Thiocapsa roseopersicina with ultrasonic wave to lyse the fungi bodies; (b) precipitating the fungi body lysate using 60% ammonium sulfate to obtain an inactive hydrogenase fraction; (c) heat-treating the fraction at a temperature of 55-65 deg.C for 15-25 minutes; (d) subjecting the fraction to an anion exchange chromatography using KCl as a solvent; (e) subjecting the fraction to a hydrophobic interaction chromatography using a 2M ammonium sulfate-added potassium phosphate buffer solution(50mM, pH 7.0); (f) subjecting the fraction to an anion exchange chromatography using 20mM Tris-HCl buffer solution as a solvent; (g) subjecting the fraction to a gel filtration chromatography using a phosphate buffer(50 mMpH 7.0) including 150 mM KCl; and (h) subjecting the fraction to a gel electrophoresis using 10% polyacrylamide gel.
Abstract translation: 提供氢化酶的制备和纯化方法，通过在乙酸盐加入的培养基中培养玫瑰聚糖硫杆菌来生产高度浓缩的氢化酶，并且从硫柳塞氏菌的培养物中纯化具有稳定活性的氢化酶。 一种生产和纯化氢化酶的方法，包括以下步骤：（a）处理通过离心通过用超声波培养玫瑰新西兰薄荷蜡获得的培养液而获得的真菌，以裂解真菌体; （b）使用60％硫酸铵沉淀真菌体裂解液以获得无活性氢化酶级分; （c）在55-65℃的温度下热处理15-25分钟; （d）使用KCl作为溶剂进行阴离子交换层析; （e）使用2M硫酸铵加入的磷酸钾缓冲溶液（50mM，pH7.0）对所述级分进行疏水相互作用层析; （f）使用20mM Tris-HCl缓冲溶液作为溶剂对该级分进行阴离子交换层析; （g）使用包含150mM KCl的磷酸盐缓冲液（50mMpH 7.0）对该级分进行凝胶过滤层析; 和（h）使用10％聚丙烯酰胺凝胶对级分进行凝胶电泳。
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